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Open data
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Basic information
Entry | Database: PDB / ID: 8jpr | ||||||
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Title | Cryo-EM structure of Y553C human ClC-6 | ||||||
![]() | H(+)/Cl(-) exchange transporter 6 | ||||||
![]() | MEMBRANE PROTEIN | ||||||
Function / homology | ![]() voltage-gated chloride channel activity / cell volume homeostasis / antiporter activity / chloride transport / chloride transmembrane transporter activity / response to mechanical stimulus / monoatomic ion transmembrane transport / Stimuli-sensing channels / Signaling by BRAF and RAF1 fusions / late endosome membrane ...voltage-gated chloride channel activity / cell volume homeostasis / antiporter activity / chloride transport / chloride transmembrane transporter activity / response to mechanical stimulus / monoatomic ion transmembrane transport / Stimuli-sensing channels / Signaling by BRAF and RAF1 fusions / late endosome membrane / endosome membrane / lysosomal membrane / intracellular membrane-bounded organelle / signal transduction / ATP binding / membrane Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ELECTRON MICROSCOPY / single particle reconstruction / cryo EM / Resolution: 3.4 Å | ||||||
![]() | Zhang, S.S. | ||||||
Funding support | ![]()
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![]() | ![]() Title: Molecular basis of ClC-6 function and its impairment in human disease Authors: Zhang, S.S. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 260.8 KB | Display | ![]() |
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PDB format | ![]() | 206.6 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Related structure data | ![]() 36487MC ![]() 8jpjC ![]() 8jpoC M: map data used to model this data C: citing same article ( |
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Similar structure data | Similarity search - Function & homology ![]() |
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Links
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Assembly
Deposited unit | ![]()
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1 |
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Components
#1: Protein | Mass: 97323.398 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() #2: Chemical | ChemComp-CL / #3: Chemical | #4: Chemical | Has ligand of interest | N | |
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-Experimental details
-Experiment
Experiment | Method: ELECTRON MICROSCOPY |
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EM experiment | Aggregation state: PARTICLE / 3D reconstruction method: single particle reconstruction |
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Sample preparation
Component | Name: endolysosomal transporter / Type: ORGANELLE OR CELLULAR COMPONENT / Entity ID: #1 / Source: RECOMBINANT |
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Molecular weight | Experimental value: NO |
Source (natural) | Organism: ![]() |
Source (recombinant) | Organism: ![]() |
Buffer solution | pH: 7.2 |
Specimen | Embedding applied: NO / Shadowing applied: NO / Staining applied: NO / Vitrification applied: YES |
Vitrification | Cryogen name: ETHANE |
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Electron microscopy imaging
Experimental equipment | ![]() Model: Titan Krios / Image courtesy: FEI Company |
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Microscopy | Model: FEI TITAN KRIOS |
Electron gun | Electron source: ![]() |
Electron lens | Mode: BRIGHT FIELD / Nominal defocus max: 2500 nm / Nominal defocus min: 1200 nm |
Image recording | Electron dose: 50 e/Å2 / Film or detector model: GATAN K3 BIOQUANTUM (6k x 4k) |
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Processing
CTF correction | Type: PHASE FLIPPING AND AMPLITUDE CORRECTION | ||||||||||||||||||||||||
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3D reconstruction | Resolution: 3.4 Å / Resolution method: FSC 0.143 CUT-OFF / Num. of particles: 134000 / Symmetry type: POINT | ||||||||||||||||||||||||
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