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Yorodumi- PDB-8izm: Structure of the Mumps Virus L Protein (state2) Bound by Phosphop... -
+Open data
-Basic information
Entry | Database: PDB / ID: 8izm | ||||||
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Title | Structure of the Mumps Virus L Protein (state2) Bound by Phosphoprotein Tetramer | ||||||
Components |
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Keywords | VIRAL PROTEIN / Mumps virus polymerase complex / RNA-dependent RNA synthesis / Large protein / phosphoprotein. | ||||||
Function / homology | Function and homology information GDP polyribonucleotidyltransferase / Hydrolases; Acting on acid anhydrides; In phosphorus-containing anhydrides / Transferases; Transferring one-carbon groups; Methyltransferases / virion component / mRNA 5'-cap (guanine-N7-)-methyltransferase activity / host cell cytoplasm / hydrolase activity / RNA-directed RNA polymerase / RNA-dependent RNA polymerase activity / ATP binding / cytoplasm Similarity search - Function | ||||||
Biological species | Mumps virus strain Jeryl Lynn | ||||||
Method | ELECTRON MICROSCOPY / single particle reconstruction / cryo EM / Resolution: 3.01 Å | ||||||
Authors | Li, T.H. / Shen, Q.T. | ||||||
Funding support | China, 1items
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Citation | Journal: To Be Published Title: Structural insights into mumps virus polymerase complex for coordinating replication and transcription Authors: Li, T.L. / Shen, Q.T. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 8izm.cif.gz | 341 KB | Display | PDBx/mmCIF format |
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PDB format | pdb8izm.ent.gz | 250.8 KB | Display | PDB format |
PDBx/mmJSON format | 8izm.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 8izm_validation.pdf.gz | 1.4 MB | Display | wwPDB validaton report |
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Full document | 8izm_full_validation.pdf.gz | 1.4 MB | Display | |
Data in XML | 8izm_validation.xml.gz | 67.4 KB | Display | |
Data in CIF | 8izm_validation.cif.gz | 98.8 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/iz/8izm ftp://data.pdbj.org/pub/pdb/validation_reports/iz/8izm | HTTPS FTP |
-Related structure data
Related structure data | 35865MC M: map data used to model this data C: citing same article (ref.) |
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Similar structure data | Similarity search - Function & homologyF&H Search |
-Links
-Assembly
Deposited unit |
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1 |
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-Components
#1: Protein | Mass: 41651.066 Da / Num. of mol.: 4 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Mumps virus strain Jeryl Lynn / Strain: Jeryl-Lynn / Cell line (production host): Sf9 / Production host: Spodoptera frugiperda (fall armyworm) / References: UniProt: Q9J4L6 #2: Protein | | Mass: 256833.094 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Mumps virus strain Jeryl Lynn / Strain: Jeryl-Lynn / Cell line (production host): Sf9 / Production host: Spodoptera frugiperda (fall armyworm) References: UniProt: Q9J4L0, RNA-directed RNA polymerase, Hydrolases; Acting on acid anhydrides; In phosphorus-containing anhydrides, GDP polyribonucleotidyltransferase, Transferases; Transferring ...References: UniProt: Q9J4L0, RNA-directed RNA polymerase, Hydrolases; Acting on acid anhydrides; In phosphorus-containing anhydrides, GDP polyribonucleotidyltransferase, Transferases; Transferring one-carbon groups; Methyltransferases #3: Chemical | Has ligand of interest | N | |
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-Experimental details
-Experiment
Experiment | Method: ELECTRON MICROSCOPY |
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EM experiment | Aggregation state: PARTICLE / 3D reconstruction method: single particle reconstruction |
-Sample preparation
Component | Name: The MuV polymerase complex of RNA-directed RNA polymerase L with tetrameric phosphoproteins Type: COMPLEX Details: The MuV polymerase complex expressed in Sf9 cells and purified by affinity chromatography and size-exlusive chromatography sequentially. Entity ID: #1-#2 / Source: RECOMBINANT | |||||||||||||||||||||||||
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Source (natural) | Organism: Mumps orthorubulavirus / Strain: Jeryl-Lynn | |||||||||||||||||||||||||
Source (recombinant) | Organism: Spodoptera frugiperda (fall armyworm) / Cell: Sf9 | |||||||||||||||||||||||||
Buffer solution | pH: 8 | |||||||||||||||||||||||||
Buffer component |
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Specimen | Conc.: 1 mg/ml / Embedding applied: NO / Shadowing applied: NO / Staining applied: NO / Vitrification applied: YES | |||||||||||||||||||||||||
Vitrification | Instrument: FEI VITROBOT MARK IV / Cryogen name: ETHANE / Humidity: 100 % / Chamber temperature: 277 K |
-Electron microscopy imaging
Experimental equipment | Model: Titan Krios / Image courtesy: FEI Company |
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Microscopy | Model: FEI TITAN KRIOS |
Electron gun | Electron source: FIELD EMISSION GUN / Accelerating voltage: 300 kV / Illumination mode: SPOT SCAN |
Electron lens | Mode: BRIGHT FIELD / Nominal defocus max: 2500 nm / Nominal defocus min: 1500 nm / Cs: 2.7 mm / Alignment procedure: COMA FREE |
Specimen holder | Cryogen: NITROGEN |
Image recording | Average exposure time: 2.75 sec. / Electron dose: 50 e/Å2 / Film or detector model: GATAN K3 BIOQUANTUM (6k x 4k) |
-Processing
CTF correction | Type: PHASE FLIPPING AND AMPLITUDE CORRECTION |
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3D reconstruction | Resolution: 3.01 Å / Resolution method: FSC 0.143 CUT-OFF / Num. of particles: 477568 / Symmetry type: POINT |