+Open data
-Basic information
Entry | Database: PDB / ID: 8ikp | ||||||
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Title | Cryo-EM structure of hnRAC1-8I fibril | ||||||
Components | GLY-PHE-GLY-GLY-ASN-ASP-ASN-PHI-GLY | ||||||
Keywords | PROTEIN FIBRIL / irreversible peptide fibril | ||||||
Biological species | Homo sapiens (human) | ||||||
Method | ELECTRON MICROSCOPY / helical reconstruction / cryo EM / Resolution: 2.98 Å | ||||||
Authors | Li, D.N. / Ma, Y.Y. / Li, D. / Dai, B. / Liu, C. | ||||||
Funding support | 1items
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Citation | Journal: To Be Published Title: Cryo-EM structure of hnRAC1-8I fibril Authors: Li, D.N. / Ma, Y.Y. / Li, D. / Dai, B. / Liu, C. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 8ikp.cif.gz | 50.2 KB | Display | PDBx/mmCIF format |
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PDB format | pdb8ikp.ent.gz | 42.3 KB | Display | PDB format |
PDBx/mmJSON format | 8ikp.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 8ikp_validation.pdf.gz | 1.4 MB | Display | wwPDB validaton report |
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Full document | 8ikp_full_validation.pdf.gz | 1.4 MB | Display | |
Data in XML | 8ikp_validation.xml.gz | 28.2 KB | Display | |
Data in CIF | 8ikp_validation.cif.gz | 39.7 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/ik/8ikp ftp://data.pdbj.org/pub/pdb/validation_reports/ik/8ikp | HTTPS FTP |
-Related structure data
-Links
-Assembly
Deposited unit |
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1 |
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-Components
#1: Protein/peptide | Mass: 1009.760 Da / Num. of mol.: 32 / Source method: obtained synthetically / Source: (synth.) Homo sapiens (human) Has ligand of interest | Y | |
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-Experimental details
-Experiment
Experiment | Method: ELECTRON MICROSCOPY |
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EM experiment | Aggregation state: FILAMENT / 3D reconstruction method: helical reconstruction |
-Sample preparation
Component | Name: Cryo-EM structure of hnRAC1-8I fibril / Type: ORGANELLE OR CELLULAR COMPONENT / Entity ID: all / Source: RECOMBINANT |
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Source (natural) | Organism: Homo sapiens (human) |
Source (recombinant) | Organism: Homo sapiens (human) |
Buffer solution | pH: 7 |
Specimen | Embedding applied: NO / Shadowing applied: NO / Staining applied: NO / Vitrification applied: YES |
Vitrification | Cryogen name: ETHANE |
-Electron microscopy imaging
Experimental equipment | Model: Titan Krios / Image courtesy: FEI Company |
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Microscopy | Model: FEI TITAN KRIOS |
Electron gun | Electron source: FIELD EMISSION GUN / Accelerating voltage: 300 kV / Illumination mode: FLOOD BEAM |
Electron lens | Mode: BRIGHT FIELD / Nominal defocus max: 2000 nm / Nominal defocus min: 1000 nm |
Image recording | Electron dose: 55 e/Å2 / Film or detector model: GATAN K3 BIOQUANTUM (6k x 4k) |
-Processing
CTF correction | Type: NONE | ||||||||||||||||||||||||
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Helical symmerty | Angular rotation/subunit: -1.06 ° / Axial rise/subunit: 4.82 Å / Axial symmetry: C1 | ||||||||||||||||||||||||
3D reconstruction | Resolution: 2.98 Å / Resolution method: FSC 0.143 CUT-OFF / Num. of particles: 10265 / Symmetry type: HELICAL | ||||||||||||||||||||||||
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