+Open data
-Basic information
Entry | Database: PDB / ID: 8iet | ||||||
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Title | Human SLC26A3 in the apo state | ||||||
Components | Chloride anion exchanger | ||||||
Keywords | MEMBRANE PROTEIN / Transporter | ||||||
Function / homology | Function and homology information Defective SLC26A3 causes congenital secretory chloride diarrhea 1 (DIAR1) / Multifunctional anion exchangers / sulfate transmembrane transporter activity / oxalate transmembrane transporter activity / secondary active sulfate transmembrane transporter activity / intracellular pH elevation / chloride:bicarbonate antiporter activity / solute:inorganic anion antiporter activity / membrane hyperpolarization / monoatomic anion transport ...Defective SLC26A3 causes congenital secretory chloride diarrhea 1 (DIAR1) / Multifunctional anion exchangers / sulfate transmembrane transporter activity / oxalate transmembrane transporter activity / secondary active sulfate transmembrane transporter activity / intracellular pH elevation / chloride:bicarbonate antiporter activity / solute:inorganic anion antiporter activity / membrane hyperpolarization / monoatomic anion transport / bicarbonate transmembrane transporter activity / chloride transmembrane transporter activity / sperm capacitation / monoatomic ion transport / cellular response to cAMP / sperm midpiece / brush border membrane / apical plasma membrane / membrane / plasma membrane Similarity search - Function | ||||||
Biological species | Homo sapiens (human) | ||||||
Method | ELECTRON MICROSCOPY / single particle reconstruction / cryo EM / Resolution: 3.5 Å | ||||||
Authors | Li, X.R. / Chi, X.M. / Zhang, Y.Y. / Zhou, Q. | ||||||
Funding support | China, 1items
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Citation | Journal: To Be Published Title: Human SLC26A3 in the apo state Authors: Li, X.R. / Chi, X.M. / Zhang, Y.Y. / Zhou, Q. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 8iet.cif.gz | 230.8 KB | Display | PDBx/mmCIF format |
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PDB format | pdb8iet.ent.gz | 184.7 KB | Display | PDB format |
PDBx/mmJSON format | 8iet.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 8iet_validation.pdf.gz | 1.2 MB | Display | wwPDB validaton report |
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Full document | 8iet_full_validation.pdf.gz | 1.2 MB | Display | |
Data in XML | 8iet_validation.xml.gz | 40.8 KB | Display | |
Data in CIF | 8iet_validation.cif.gz | 61.7 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/ie/8iet ftp://data.pdbj.org/pub/pdb/validation_reports/ie/8iet | HTTPS FTP |
-Related structure data
Related structure data | 35393MC M: map data used to model this data C: citing same article (ref.) |
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Similar structure data | Similarity search - Function & homologyF&H Search |
-Links
-Assembly
Deposited unit |
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1 |
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-Components
#1: Protein | Mass: 84591.523 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Gene: SLC26A3, DRA / Production host: Homo sapiens (human) / References: UniProt: P40879 |
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-Experimental details
-Experiment
Experiment | Method: ELECTRON MICROSCOPY |
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EM experiment | Aggregation state: PARTICLE / 3D reconstruction method: single particle reconstruction |
-Sample preparation
Component | Name: Human SLC26A3 in the apo state / Type: CELL / Entity ID: all / Source: NATURAL |
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Source (natural) | Organism: Homo sapiens (human) |
Source (recombinant) | Organism: Homo sapiens (human) |
Buffer solution | pH: 7.4 |
Specimen | Embedding applied: NO / Shadowing applied: NO / Staining applied: NO / Vitrification applied: YES |
Vitrification | Cryogen name: ETHANE |
-Electron microscopy imaging
Experimental equipment | Model: Titan Krios / Image courtesy: FEI Company |
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Microscopy | Model: FEI TITAN KRIOS |
Electron gun | Electron source: FIELD EMISSION GUN / Accelerating voltage: 300 kV / Illumination mode: FLOOD BEAM |
Electron lens | Mode: BRIGHT FIELD / Nominal defocus max: 2200 nm / Nominal defocus min: 1200 nm |
Image recording | Electron dose: 50 e/Å2 / Film or detector model: GATAN K3 (6k x 4k) |
-Processing
EM software | Name: RELION / Category: 3D reconstruction |
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CTF correction | Type: PHASE FLIPPING AND AMPLITUDE CORRECTION |
3D reconstruction | Resolution: 3.5 Å / Resolution method: FSC 0.143 CUT-OFF / Num. of particles: 142727 / Symmetry type: POINT |