+Open data
-Basic information
Entry | Database: PDB / ID: 8hvt | ||||||
---|---|---|---|---|---|---|---|
Title | Structure of the human CLC-7/Ostm1 complex reveals a novel state | ||||||
Components |
| ||||||
Keywords | TRANSPORT PROTEIN / chloride transporter / CLC-7 / Ostm1 | ||||||
Function / homology | Function and homology information transepithelial chloride transport / chloride channel complex / osteoclast differentiation / Stimuli-sensing channels / lysosomal membrane / cytosol Similarity search - Function | ||||||
Biological species | Homo sapiens (human) | ||||||
Method | ELECTRON MICROSCOPY / single particle reconstruction / cryo EM / Resolution: 3.6 Å | ||||||
Authors | Zhang, Z.X. / Chen, L. / He, J. / She, J. | ||||||
Funding support | China, 1items
| ||||||
Citation | Journal: JUSTC / Year: 2023 Title: Structure of the human CLC-7/Ostm1 complex reveals a novel state Authors: Zhang, Z.X. / Chen, L. / He, J. / She, J. | ||||||
History |
|
-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
---|
-Downloads & links
-Download
PDBx/mmCIF format | 8hvt.cif.gz | 258.5 KB | Display | PDBx/mmCIF format |
---|---|---|---|---|
PDB format | pdb8hvt.ent.gz | 198.9 KB | Display | PDB format |
PDBx/mmJSON format | 8hvt.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 8hvt_validation.pdf.gz | 1.5 MB | Display | wwPDB validaton report |
---|---|---|---|---|
Full document | 8hvt_full_validation.pdf.gz | 1.5 MB | Display | |
Data in XML | 8hvt_validation.xml.gz | 45.6 KB | Display | |
Data in CIF | 8hvt_validation.cif.gz | 68.4 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/hv/8hvt ftp://data.pdbj.org/pub/pdb/validation_reports/hv/8hvt | HTTPS FTP |
-Related structure data
Related structure data | 35048MC M: map data used to model this data C: citing same article (ref.) |
---|---|
Similar structure data | Similarity search - Function & homologyF&H Search |
-Links
-Assembly
Deposited unit |
|
---|---|
1 |
|
-Components
#1: Protein | Mass: 88773.875 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Gene: CLCN7 / Production host: Homo sapiens (human) / References: UniProt: P51798 #2: Protein | Mass: 37290.570 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Gene: OSTM1, GL, HSPC019, UNQ6098/PRO21201 / Production host: Homo sapiens (human) / References: UniProt: Q86WC4 #3: Polysaccharide | #4: Sugar | ChemComp-NAG / Has ligand of interest | Y | |
---|
-Experimental details
-Experiment
Experiment | Method: ELECTRON MICROSCOPY |
---|---|
EM experiment | Aggregation state: PARTICLE / 3D reconstruction method: single particle reconstruction |
-Sample preparation
Component | Name: CLC-7/Ostm1 / Type: COMPLEX / Entity ID: #1-#2 / Source: RECOMBINANT |
---|---|
Source (natural) | Organism: Homo sapiens (human) |
Source (recombinant) | Organism: Homo sapiens (human) |
Buffer solution | pH: 8 |
Specimen | Conc.: 4.2 mg/ml / Embedding applied: NO / Shadowing applied: NO / Staining applied: NO / Vitrification applied: YES |
Vitrification | Cryogen name: ETHANE |
-Electron microscopy imaging
Experimental equipment | Model: Titan Krios / Image courtesy: FEI Company |
---|---|
Microscopy | Model: FEI TITAN KRIOS |
Electron gun | Electron source: FIELD EMISSION GUN / Accelerating voltage: 300 kV / Illumination mode: FLOOD BEAM |
Electron lens | Mode: BRIGHT FIELD / Nominal defocus max: 2800 nm / Nominal defocus min: 1200 nm |
Image recording | Electron dose: 64 e/Å2 / Film or detector model: GATAN K3 (6k x 4k) |
-Processing
Software | Name: PHENIX / Version: 1.18.2_3874: / Classification: refinement | ||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
CTF correction | Type: NONE | ||||||||||||||||||||||||
3D reconstruction | Resolution: 3.6 Å / Resolution method: FSC 0.143 CUT-OFF / Num. of particles: 199000 / Symmetry type: POINT | ||||||||||||||||||||||||
Refine LS restraints |
|