Electron dose: 30 e/Å2 / Detector mode: COUNTING / Film or detector model: GATAN K3 (6k x 4k) / Num. of real images: 8640
-
Processing
EM software
ID
Name
Version
Category
Details
4
CTFFIND
4.1
CTFcorrection
Forinitialestimation
5
RELION
3.1.0
CTFcorrection
CTFRefinement
8
Coot
0.9.8.1
modelfitting
10
PHENIX
1.20.1
modelrefinement
11
ISOLDE
1.3
modelrefinement
12
RELION
3.1.0
initialEulerassignment
13
RELION
3.1.0
finalEulerassignment
14
RELION
3.1.0
classification
15
RELION
3.1.0
3Dreconstruction
CTF correction
Details: Standard CTF correction inside RELION's reconstruction. Type: PHASE FLIPPING AND AMPLITUDE CORRECTION
Symmetry
Point symmetry: I (icosahedral)
3D reconstruction
Resolution: 2.39 Å / Resolution method: FSC 0.143 CUT-OFF / Num. of particles: 61957 / Symmetry type: POINT
Atomic model building
Protocol: AB INITIO MODEL Details: Amino acid sequence built into the map for a single major capsid protein and refined with Phenix. Model then used for rest of asymmetric unit and refined with Phenix. Final step involved using Isolde.
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