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- PDB-8fru: 60S subunit of the Giardia lamblia 80S ribosome -

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Basic information

Entry
Database: PDB / ID: 8fru
Title60S subunit of the Giardia lamblia 80S ribosome
Components
  • (60S ribosomal protein ...) x 40
  • 28S rRNA
  • 5.8S rRNA
  • 5S rRNA
KeywordsRIBOSOME / Giardia lamblia / Ribosome structure / Translation / parasite
Function / homology
Function and homology information


maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / maturation of LSU-rRNA / ribosomal large subunit biogenesis / modification-dependent protein catabolic process / large ribosomal subunit / protein tag activity / ribosome biogenesis / 5S rRNA binding / large ribosomal subunit rRNA binding / cytosolic large ribosomal subunit ...maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / maturation of LSU-rRNA / ribosomal large subunit biogenesis / modification-dependent protein catabolic process / large ribosomal subunit / protein tag activity / ribosome biogenesis / 5S rRNA binding / large ribosomal subunit rRNA binding / cytosolic large ribosomal subunit / cytoplasmic translation / rRNA binding / negative regulation of translation / ribosome / protein ubiquitination / structural constituent of ribosome / ribonucleoprotein complex / translation / mRNA binding / ubiquitin protein ligase binding / nucleolus / RNA binding / metal ion binding
Similarity search - Function
Ribosomal protein L40e, archaeal / Ribosomal protein L29e / Ribosomal L29e protein family / Ribosomal protein L13e, conserved site / Ribosomal protein L13e signature. / Ribosomal protein L22e / Ribosomal protein L22e superfamily / Ribosomal L22e protein family / Ribosomal protein L38e / Ribosomal protein L38e superfamily ...Ribosomal protein L40e, archaeal / Ribosomal protein L29e / Ribosomal L29e protein family / Ribosomal protein L13e, conserved site / Ribosomal protein L13e signature. / Ribosomal protein L22e / Ribosomal protein L22e superfamily / Ribosomal L22e protein family / Ribosomal protein L38e / Ribosomal protein L38e superfamily / Ribosomal L38e protein family / Ribosomal protein L10e, conserved site / Ribosomal protein L10e signature. / Ribosomal protein L10e / Ribosomal protein L13e / Ribosomal protein L13e / 60S ribosomal protein L18a/ L20, eukaryotes / Ribosomal protein L44e / Ribosomal protein L44 / Ribosomal protein L5 eukaryotic, C-terminal / Ribosomal L18 C-terminal region / 50S ribosomal protein L18Ae/60S ribosomal protein L20 and L18a / Ribosomal L40e family / Ribosomal protein 50S-L18Ae/60S-L20/60S-L18A / Ribosomal proteins 50S-L18Ae/60S-L20/60S-L18A / Ribosomal_L40e / Ribosomal protein L40e / Ribosomal protein L40e superfamily / Ribosomal protein L23/L25, N-terminal / Ribosomal protein L23, N-terminal domain / Ribosomal protein 60S L18 and 50S L18e / Eukaryotic Ribosomal Protein L27, KOW domain / Ribosomal protein L27e / Ribosomal protein L27e superfamily / Ribosomal L27e protein family / 60S ribosomal protein L19 / Ribosomal protein L34Ae / Ribosomal protein L34e / 60S ribosomal protein L35 / Ribosomal protein L35Ae, conserved site / Ribosomal protein L35Ae signature. / Ribosomal protein L18/L18-A/B/e, conserved site / Ribosomal protein L18e signature. / Ribosomal protein L30/YlxQ / Ribosomal protein L7A/L8 / Ribosomal protein L13, eukaryotic/archaeal / Ribosomal protein L18e / 60S ribosomal protein L4, C-terminal domain / 60S ribosomal protein L4 C-terminal domain / Ribosomal_L19e / Ribosomal protein L19/L19e / Ribosomal protein L37ae / Ribosomal protein L19/L19e, domain 1 / Ribosomal protein L31e, conserved site / Ribosomal protein L19/L19e superfamily / Ribosomal protein L19e / Ribosomal L37ae protein family / Ribosomal protein L31e signature. / Ribosomal protein L36e / Ribosomal protein L36e domain superfamily / Ribosomal protein L36e / Ribosomal protein L14e domain / Ribosomal protein L14 / Ribosomal protein L5 eukaryotic/L18 archaeal / Ribosomal large subunit proteins 60S L5, and 50S L18 / Ribosomal protein L35A / Ribosomal protein L35Ae / Ribosomal protein L35A superfamily / Ribosomal protein L32e, conserved site / Ribosomal protein L32e signature. / Ribosomal protein L14 / Ribosomal protein L14, KOW motif / Ribosomal protein L4/L1e, eukaryotic/archaeal, conserved site / Ribosomal protein L1e signature. / Ribosomal protein L31e / Ribosomal protein L15e, conserved site / Ribosomal protein L31e domain superfamily / Ribosomal protein L31e / Ribosomal protein L15e signature. / Ribosomal_L31e / Ribosomal protein L21e / Ribosomal protein L21e, conserved site / Ribosomal protein L21 superfamily / Ribosomal protein L21e / Ribosomal protein L21e signature. / Ribosomal protein L4, eukaryotic and archaeal type / Ribosomal protein L22/L17, eukaryotic/archaeal / Ribosomal protein L3, domain 3, archaeal type superfamily / Ribosomal protein L3, archaeal/eukaryotic type / Ribosomal protein L37e, conserved site / Ribosomal protein L37e signature. / Ribosomal protein L24e-related / Ribosomal protein L24e/L24 superfamily / Ribosomal protein L24e / Ribosomal protein L37e / Ribosomal protein L37e / Ribosomal_L15e / Ribosomal protein L15e / Ribosomal protein L15e core domain superfamily / Ribosomal L15
Similarity search - Domain/homology
: / SPERMIDINE / : / : / : / RNA / RNA (> 10) / RNA (> 100) / RNA (> 1000) / LSU ribosomal protein L29P/L35 ...: / SPERMIDINE / : / : / : / RNA / RNA (> 10) / RNA (> 100) / RNA (> 1000) / LSU ribosomal protein L29P/L35 / Ribosomal protein L27 / Ribosomal protein L21 / Ribosomal L38e / Ribosomal protein L14 / Ribosomal protein L18 / Ribosomal protein L30 / 60S ribosomal protein L13 / Ribosomal protein L4 / Ribosomal protein L15 / Ribosomal protein L17 / Ribosomal protein L19 / Ribosomal protein L11 / Ribosomal protein L35a / 60S ribosomal protein L7a / Ribosomal protein L10 / Ribosomal protein L32 / Large ribosomal subunit protein eL34 / Ribosomal protein L31B / Ribosomal protein L23A / Ribosomal protein L37a / Ribosomal protein L6 / Ribosomal protein L44 / Ribosomal protein L24A / Ribosomal protein L7 / Ribosomal protein L23 / Ribosomal protein L13a / Large ribosomal subunit protein eL22 / 50S ribosomal protein L40e / Ribosomal protein L36-1 / Ribosomal protein L27a / Ribosomal protein L2 / Ribosomal protein L5 / 60S ribosomal protein L29 / LSU ribosomal protein L18AE / LSU ribosomal protein L24P / LSU ribosomal protein L3P / Ribosomal protein L37
Similarity search - Component
Biological speciesGiardia intestinalis assemblage A (eukaryote)
MethodELECTRON MICROSCOPY / single particle reconstruction / cryo EM / Resolution: 2.49 Å
AuthorsEiler, D.R. / Wimberly, B.T. / Bilodeau, D.Y. / Rissland, O.S. / Kieft, J.S.
Funding support United States, 3items
OrganizationGrant numberCountry
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)R35GM118070 United States
National Institutes of Health/National Institute Of Allergy and Infectious Diseases (NIH/NIAID)1R21AI149210 United States
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)R35GM128680 United States
CitationJournal: Structure / Year: 2024
Title: The Giardia lamblia ribosome structure reveals divergence in several biological pathways and the mode of emetine function.
Authors: Daniel R Eiler / Brian T Wimberly / Danielle Y Bilodeau / J Matthew Taliaferro / Philip Reigan / Olivia S Rissland / Jeffrey S Kieft /
Abstract: Giardia lamblia is a deeply branching protist and a human pathogen. Its unusual biology presents the opportunity to explore conserved and fundamental molecular mechanisms. We determined the structure ...Giardia lamblia is a deeply branching protist and a human pathogen. Its unusual biology presents the opportunity to explore conserved and fundamental molecular mechanisms. We determined the structure of the G. lamblia 80S ribosome bound to tRNA, mRNA, and the antibiotic emetine by cryo-electron microscopy, to an overall resolution of 2.49 Å. The structure reveals rapidly evolving protein and nucleotide regions, differences in the peptide exit tunnel, and likely altered ribosome quality control pathways. Examination of translation initiation factor binding sites suggests these interactions are conserved despite a divergent initiation mechanism. Highlighting the potential of G. lamblia to resolve conserved biological principles; our structure reveals the interactions of the translation inhibitor emetine with the ribosome and mRNA, thus providing insight into the mechanism of action for this widely used antibiotic. Our work defines key questions in G. lamblia and motivates future experiments to explore the diversity of eukaryotic gene regulation.
History
DepositionJan 8, 2023Deposition site: RCSB / Processing site: RCSB
Revision 1.0Jan 31, 2024Provider: repository / Type: Initial release
Revision 1.1Apr 17, 2024Group: Database references / Category: citation / Item: _citation.journal_volume / _citation.page_first

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
B: 60S ribosomal protein uL3
a: 60S ribosomal protein uL15
C: 60S ribosomal protein uL4
I: 60S ribosomal protein uL16
p: 60S ribosomal protein eL43
D: 60S ribosomal protein uL18
H: 60S ribosomal protein uL6
G: 60S ribosomal protein eL8
J: 60S ribosomal protein uL5
O: 60S ribosomal protein uL13
L: 60S ribosomal protein eL13
M: 60S ribosomal protein eL14
N: 60S ribosomal protein eL15
Q: 60S ribosomal protein eL18
S: 60S ribosomal protein eL20
R: 60S ribosomal protein eL19
T: 60S ribosomal protein eL21
V: 60S ribosomal protein uL14
P: 60S ribosomal protein uL22
X: 60S ribosomal protein uL23
Y: 60S ribosomal protein uL24
W: 60S ribosomal protein eL24
Z: 60S ribosomal protein eL27
b: 60S ribosomal protein eL29
h: 60S ribosomal protein uL29
F: 60S ribosomal protein uL30
d: 60S ribosomal protein eL31
e: 60S ribosomal protein eL32
g: 60S ribosomal protein eL34
f: 60S ribosomal protein eL33
i: 60S ribosomal protein eL36
k: 60S ribosomal protein eL38
o: 60S ribosomal protein eL42
U: 60S ribosomal protein eL22
c: 60S ribosomal protein eL30
j: 60S ribosomal protein eL37
l: 60S ribosomal protein eL39
m: 60S ribosomal protein eL40
A: 60S ribosomal protein uL2
n: 60S ribosomal protein eL41
3: 5S rRNA
4: 5.8S rRNA
1: 28S rRNA
hetero molecules


Theoretical massNumber of molelcules
Total (without water)1,687,717235
Polymers1,682,49143
Non-polymers5,226192
Water56,2433122
1


  • Idetical with deposited unit
  • defined by author
  • Evidence: mass spectrometry
TypeNameSymmetry operationNumber
identity operation1_5551

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Components

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60S ribosomal protein ... , 40 types, 40 molecules BaCIpDHGJOLMNQSRTVPXYWZbhFdegf...

#1: Protein 60S ribosomal protein uL3 / LSU ribosomal protein L3P


Mass: 42688.520 Da / Num. of mol.: 1 / Source method: isolated from a natural source
Source: (natural) Giardia intestinalis assemblage A (eukaryote)
References: UniProt: V6TF11
#2: Protein 60S ribosomal protein uL15


Mass: 17166.957 Da / Num. of mol.: 1 / Source method: isolated from a natural source
Source: (natural) Giardia intestinalis assemblage A (eukaryote)
References: UniProt: A8BZ78
#3: Protein 60S ribosomal protein uL4


Mass: 35048.707 Da / Num. of mol.: 1 / Source method: isolated from a natural source
Source: (natural) Giardia intestinalis assemblage A (eukaryote)
References: UniProt: A8B7H8
#4: Protein 60S ribosomal protein uL16


Mass: 24283.172 Da / Num. of mol.: 1 / Source method: isolated from a natural source
Source: (natural) Giardia intestinalis assemblage A (eukaryote)
References: UniProt: A8BM86
#5: Protein 60S ribosomal protein eL43


Mass: 10508.366 Da / Num. of mol.: 1 / Source method: isolated from a natural source
Source: (natural) Giardia intestinalis assemblage A (eukaryote)
References: UniProt: A8BN26
#6: Protein 60S ribosomal protein uL18


Mass: 33961.375 Da / Num. of mol.: 1 / Source method: isolated from a natural source
Source: (natural) Giardia intestinalis assemblage A (eukaryote)
References: UniProt: E2RU47
#7: Protein 60S ribosomal protein uL6


Mass: 20889.379 Da / Num. of mol.: 1 / Source method: isolated from a natural source
Source: (natural) Giardia intestinalis assemblage A (eukaryote)
References: UniProt: A8BNT5
#8: Protein 60S ribosomal protein eL8


Mass: 25427.725 Da / Num. of mol.: 1 / Source method: isolated from a natural source
Source: (natural) Giardia intestinalis assemblage A (eukaryote)
References: UniProt: A8BLL7
#9: Protein 60S ribosomal protein uL5


Mass: 19767.684 Da / Num. of mol.: 1 / Source method: isolated from a natural source
Source: (natural) Giardia intestinalis assemblage A (eukaryote)
References: UniProt: A8BI95
#10: Protein 60S ribosomal protein uL13


Mass: 22921.914 Da / Num. of mol.: 1 / Source method: isolated from a natural source
Source: (natural) Giardia intestinalis assemblage A (eukaryote)
References: UniProt: A8BU75
#11: Protein 60S ribosomal protein eL13


Mass: 26640.346 Da / Num. of mol.: 1 / Source method: isolated from a natural source
Source: (natural) Giardia intestinalis assemblage A (eukaryote)
References: UniProt: A8B6M8
#12: Protein 60S ribosomal protein eL14


Mass: 14785.472 Da / Num. of mol.: 1 / Source method: isolated from a natural source
Source: (natural) Giardia intestinalis assemblage A (eukaryote)
References: UniProt: A8B4F8
#13: Protein 60S ribosomal protein eL15


Mass: 24353.730 Da / Num. of mol.: 1 / Source method: isolated from a natural source
Source: (natural) Giardia intestinalis assemblage A (eukaryote)
References: UniProt: A8B8Z6
#14: Protein 60S ribosomal protein eL18


Mass: 20207.768 Da / Num. of mol.: 1 / Source method: isolated from a natural source
Source: (natural) Giardia intestinalis assemblage A (eukaryote)
References: UniProt: A8B4T7
#15: Protein 60S ribosomal protein eL20


Mass: 24389.225 Da / Num. of mol.: 1 / Source method: isolated from a natural source
Source: (natural) Giardia intestinalis assemblage A (eukaryote)
References: UniProt: V6TB90
#16: Protein 60S ribosomal protein eL19


Mass: 23199.238 Da / Num. of mol.: 1 / Source method: isolated from a natural source
Source: (natural) Giardia intestinalis assemblage A (eukaryote)
References: UniProt: A8BH61
#17: Protein 60S ribosomal protein eL21


Mass: 18296.393 Da / Num. of mol.: 1 / Source method: isolated from a natural source
Source: (natural) Giardia intestinalis assemblage A (eukaryote)
References: UniProt: A8B2Q4
#18: Protein 60S ribosomal protein uL14


Mass: 15450.040 Da / Num. of mol.: 1 / Source method: isolated from a natural source
Source: (natural) Giardia intestinalis assemblage A (eukaryote)
References: UniProt: A8BS37
#19: Protein 60S ribosomal protein uL22


Mass: 18722.785 Da / Num. of mol.: 1 / Source method: isolated from a natural source
Source: (natural) Giardia intestinalis assemblage A (eukaryote)
References: UniProt: A8BE76
#20: Protein 60S ribosomal protein uL23


Mass: 16053.919 Da / Num. of mol.: 1 / Source method: isolated from a natural source
Source: (natural) Giardia intestinalis assemblage A (eukaryote)
References: UniProt: A8BMP6
#21: Protein 60S ribosomal protein uL24


Mass: 15621.398 Da / Num. of mol.: 1 / Source method: isolated from a natural source
Source: (natural) Giardia intestinalis assemblage A (eukaryote)
References: UniProt: V6TD19
#22: Protein 60S ribosomal protein eL24


Mass: 12005.191 Da / Num. of mol.: 1 / Source method: isolated from a natural source
Source: (natural) Giardia intestinalis assemblage A (eukaryote)
References: UniProt: A8BRH7
#23: Protein 60S ribosomal protein eL27


Mass: 15374.895 Da / Num. of mol.: 1 / Source method: isolated from a natural source
Source: (natural) Giardia intestinalis assemblage A (eukaryote)
References: UniProt: A8B1T3
#24: Protein 60S ribosomal protein eL29


Mass: 7363.574 Da / Num. of mol.: 1 / Source method: isolated from a natural source
Source: (natural) Giardia intestinalis assemblage A (eukaryote)
References: UniProt: V6TAA6
#25: Protein 60S ribosomal protein uL29


Mass: 14316.314 Da / Num. of mol.: 1 / Source method: isolated from a natural source
Source: (natural) Giardia intestinalis assemblage A (eukaryote)
References: UniProt: A0A132NRN1
#26: Protein 60S ribosomal protein uL30


Mass: 27103.406 Da / Num. of mol.: 1 / Source method: isolated from a natural source
Source: (natural) Giardia intestinalis assemblage A (eukaryote)
References: UniProt: A8BRY6
#27: Protein 60S ribosomal protein eL31


Mass: 12184.064 Da / Num. of mol.: 1 / Source method: isolated from a natural source
Source: (natural) Giardia intestinalis assemblage A (eukaryote)
References: UniProt: A8BMP3
#28: Protein 60S ribosomal protein eL32


Mass: 15985.983 Da / Num. of mol.: 1 / Source method: isolated from a natural source
Source: (natural) Giardia intestinalis assemblage A (eukaryote)
References: UniProt: A8BMD9
#29: Protein 60S ribosomal protein eL34


Mass: 13528.753 Da / Num. of mol.: 1 / Source method: isolated from a natural source
Source: (natural) Giardia intestinalis assemblage A (eukaryote)
References: UniProt: A8BMF7
#30: Protein 60S ribosomal protein eL33


Mass: 13913.122 Da / Num. of mol.: 1 / Source method: isolated from a natural source
Source: (natural) Giardia intestinalis assemblage A (eukaryote)
References: UniProt: A8BKK5
#31: Protein 60S ribosomal protein eL36


Mass: 10586.708 Da / Num. of mol.: 1 / Source method: isolated from a natural source
Source: (natural) Giardia intestinalis assemblage A (eukaryote)
References: UniProt: A8BYA6
#32: Protein 60S ribosomal protein eL38


Mass: 8605.145 Da / Num. of mol.: 1 / Source method: isolated from a natural source
Source: (natural) Giardia intestinalis assemblage A (eukaryote)
References: UniProt: A8B481
#33: Protein 60S ribosomal protein eL42


Mass: 12311.678 Da / Num. of mol.: 1 / Source method: isolated from a natural source
Source: (natural) Giardia intestinalis assemblage A (eukaryote)
References: UniProt: A8BQU8
#34: Protein 60S ribosomal protein eL22


Mass: 19942.402 Da / Num. of mol.: 1 / Source method: isolated from a natural source
Source: (natural) Giardia intestinalis assemblage A (eukaryote)
References: UniProt: A8BW84
#35: Protein 60S ribosomal protein eL30


Mass: 11698.598 Da / Num. of mol.: 1 / Source method: isolated from a natural source
Source: (natural) Giardia intestinalis assemblage A (eukaryote)
References: UniProt: A8B5N7
#36: Protein 60S ribosomal protein eL37


Mass: 11473.345 Da / Num. of mol.: 1 / Source method: isolated from a natural source
Source: (natural) Giardia intestinalis assemblage A (eukaryote)
References: UniProt: V6TG99
#37: Protein 60S ribosomal protein eL39


Mass: 6527.003 Da / Num. of mol.: 1 / Source method: isolated from a natural source
Source: (natural) Giardia intestinalis assemblage A (eukaryote)
#38: Protein 60S ribosomal protein eL40


Mass: 14343.675 Da / Num. of mol.: 1 / Source method: isolated from a natural source
Source: (natural) Giardia intestinalis assemblage A (eukaryote)
References: UniProt: A8BXE1
#39: Protein 60S ribosomal protein uL2


Mass: 27090.543 Da / Num. of mol.: 1 / Source method: isolated from a natural source
Source: (natural) Giardia intestinalis assemblage A (eukaryote)
References: UniProt: E2RTN4
#40: Protein/peptide 60S ribosomal protein eL41


Mass: 2145.636 Da / Num. of mol.: 1 / Source method: isolated from a natural source
Source: (natural) Giardia intestinalis assemblage A (eukaryote)

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RNA chain , 3 types, 3 molecules 341

#41: RNA chain 5S rRNA


Mass: 38672.023 Da / Num. of mol.: 1 / Source method: isolated from a natural source
Source: (natural) Giardia intestinalis assemblage A (eukaryote)
References: GenBank: 1940868085
#42: RNA chain 5.8S rRNA


Mass: 45586.270 Da / Num. of mol.: 1 / Source method: isolated from a natural source
Source: (natural) Giardia intestinalis assemblage A (eukaryote)
References: GenBank: 583967408
#43: RNA chain 28S rRNA


Mass: 871348.812 Da / Num. of mol.: 1 / Source method: isolated from a natural source
Source: (natural) Giardia intestinalis assemblage A (eukaryote)
References: GenBank: 2333213660

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Non-polymers , 6 types, 3314 molecules

#44: Chemical...
ChemComp-K / POTASSIUM ION


Mass: 39.098 Da / Num. of mol.: 27 / Source method: obtained synthetically / Formula: K
#45: Chemical...
ChemComp-MG / MAGNESIUM ION


Mass: 24.305 Da / Num. of mol.: 129 / Source method: obtained synthetically / Formula: Mg
#46: Chemical ChemComp-ZN / ZINC ION


Mass: 65.409 Da / Num. of mol.: 2 / Source method: obtained synthetically / Formula: Zn
#47: Chemical...
ChemComp-NA / SODIUM ION


Mass: 22.990 Da / Num. of mol.: 33 / Source method: obtained synthetically / Formula: Na
#48: Chemical ChemComp-SPD / SPERMIDINE / N-(2-AMINO-PROPYL)-1,4-DIAMINOBUTANE / PA(34)


Mass: 145.246 Da / Num. of mol.: 1 / Source method: obtained synthetically / Formula: C7H19N3
#49: Water ChemComp-HOH / water


Mass: 18.015 Da / Num. of mol.: 3122 / Source method: isolated from a natural source / Formula: H2O

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Details

Has ligand of interestN

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Experimental details

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Experiment

ExperimentMethod: ELECTRON MICROSCOPY
EM experimentAggregation state: PARTICLE / 3D reconstruction method: single particle reconstruction

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Sample preparation

ComponentName: 60S ribosomal subunit of the 80S Giardia intestinalis assemblage A ribosome
Type: RIBOSOME / Entity ID: #1-#43 / Source: NATURAL
Source (natural)Organism: Giardia intestinalis assemblage A (eukaryote)
Buffer solutionpH: 7.4
SpecimenEmbedding applied: NO / Shadowing applied: NO / Staining applied: NO / Vitrification applied: YES
VitrificationCryogen name: ETHANE

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Electron microscopy imaging

Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company
MicroscopyModel: TFS KRIOS
Electron gunElectron source: FIELD EMISSION GUN / Accelerating voltage: 300 kV / Illumination mode: FLOOD BEAM
Electron lensMode: BRIGHT FIELD / Nominal defocus max: 1900 nm / Nominal defocus min: 500 nm
Image recordingElectron dose: 72.26 e/Å2 / Film or detector model: GATAN K3 (6k x 4k)

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Processing

CTF correctionType: PHASE FLIPPING AND AMPLITUDE CORRECTION
3D reconstructionResolution: 2.49 Å / Resolution method: FSC 0.143 CUT-OFF / Num. of particles: 289618 / Symmetry type: POINT
Refine LS restraints
Refine-IDTypeDev idealNumber
ELECTRON MICROSCOPYf_bond_d0.006115149
ELECTRON MICROSCOPYf_angle_d0.663169232
ELECTRON MICROSCOPYf_dihedral_angle_d13.13240325
ELECTRON MICROSCOPYf_chiral_restr0.04320970
ELECTRON MICROSCOPYf_plane_restr0.00611046

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