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Open data
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Basic information
Entry | Database: PDB / ID: 8e4g | ||||||
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Title | Remodeling of the bacteriophage T7 during initial infection | ||||||
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![]() | VIRUS / T7 / tail machine / adsorption / genome ejection | ||||||
Function / homology | ![]() host cell periplasmic space / virus tail, tube / viral portal complex / symbiont genome ejection through host cell envelope, short tail mechanism / virus tail, fiber / viral DNA genome packaging / adhesion receptor-mediated virion attachment to host cell / virion component / symbiont entry into host cell / virion attachment to host cell ...host cell periplasmic space / virus tail, tube / viral portal complex / symbiont genome ejection through host cell envelope, short tail mechanism / virus tail, fiber / viral DNA genome packaging / adhesion receptor-mediated virion attachment to host cell / virion component / symbiont entry into host cell / virion attachment to host cell / host cell plasma membrane / identical protein binding / membrane Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ELECTRON MICROSCOPY / single particle reconstruction / cryo EM / Resolution: 3.2 Å | ||||||
![]() | Wang, C. / Liu, J. / Molineux, I.J. | ||||||
Funding support | ![]()
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![]() | ![]() Title: Virion remodeling of bacteriophage T7 during infection initiation Authors: Wang, C. / Park, T. / Liu, J. / Molineux, I.J. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 897.9 KB | Display | ![]() |
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PDB format | ![]() | 753 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 1.1 MB | Display | ![]() |
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Full document | ![]() | 1.1 MB | Display | |
Data in XML | ![]() | 80.6 KB | Display | |
Data in CIF | ![]() | 120.7 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 27595MC M: map data used to model this data C: citing same article ( |
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Similar structure data | Similarity search - Function & homology ![]() |
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Links
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Assembly
Deposited unit | ![]()
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Components
-Protein , 2 types, 5 molecules ruOYg
#1: Protein | Mass: 55414.727 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) ![]() ![]() #3: Protein | Mass: 18604.920 Da / Num. of mol.: 3 / Source method: isolated from a natural source / Source: (natural) ![]() ![]() |
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-Tail tubular protein ... , 2 types, 3 molecules 05P
#2: Protein | Mass: 22307.650 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) ![]() ![]() #4: Protein | | Mass: 89563.664 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) ![]() ![]() |
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-Internal virion protein ... , 2 types, 2 molecules ax
#5: Protein | Mass: 14016.699 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) ![]() ![]() |
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#6: Protein | Mass: 5659.422 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) ![]() ![]() |
-Experimental details
-Experiment
Experiment | Method: ELECTRON MICROSCOPY |
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EM experiment | Aggregation state: PARTICLE / 3D reconstruction method: single particle reconstruction |
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Sample preparation
Component | Name: Escherichia phage T7 / Type: VIRUS / Entity ID: all / Source: NATURAL |
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Source (natural) | Organism: ![]() ![]() |
Details of virus | Empty: NO / Enveloped: NO / Isolate: OTHER / Type: VIRION |
Buffer solution | pH: 7.5 |
Specimen | Embedding applied: NO / Shadowing applied: NO / Staining applied: NO / Vitrification applied: YES |
Vitrification | Cryogen name: ETHANE |
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Electron microscopy imaging
Experimental equipment | ![]() Model: Titan Krios / Image courtesy: FEI Company |
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Microscopy | Model: FEI TITAN KRIOS |
Electron gun | Electron source: ![]() |
Electron lens | Mode: BRIGHT FIELD / Nominal defocus max: 1200 nm / Nominal defocus min: 600 nm |
Image recording | Electron dose: 30 e/Å2 / Film or detector model: GATAN K3 (6k x 4k) |
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Processing
CTF correction | Type: PHASE FLIPPING AND AMPLITUDE CORRECTION | ||||||||||||||||||||||||
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3D reconstruction | Resolution: 3.2 Å / Resolution method: FSC 0.143 CUT-OFF / Num. of particles: 29784 / Symmetry type: POINT | ||||||||||||||||||||||||
Refine LS restraints |
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