+Open data
-Basic information
Entry | Database: PDB / ID: 8dmi | ||||||||||||||||||||||||||||||
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Title | Lymphocytic choriomeningitis virus glycoprotein | ||||||||||||||||||||||||||||||
Components |
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Keywords | VIRAL PROTEIN / Viral glycoprotein / arenavirus / LCMV / GP | ||||||||||||||||||||||||||||||
Function / homology | Function and homology information corrinoid adenosyltransferase activity / host cell Golgi membrane / receptor-mediated endocytosis of virus by host cell / host cell endoplasmic reticulum membrane / fusion of virus membrane with host endosome membrane / viral envelope / virion attachment to host cell / host cell plasma membrane / virion membrane / ATP binding ...corrinoid adenosyltransferase activity / host cell Golgi membrane / receptor-mediated endocytosis of virus by host cell / host cell endoplasmic reticulum membrane / fusion of virus membrane with host endosome membrane / viral envelope / virion attachment to host cell / host cell plasma membrane / virion membrane / ATP binding / membrane / metal ion binding Similarity search - Function | ||||||||||||||||||||||||||||||
Biological species | Lymphocytic choriomeningitis virus | ||||||||||||||||||||||||||||||
Method | ELECTRON MICROSCOPY / single particle reconstruction / cryo EM / Resolution: 3.26 Å | ||||||||||||||||||||||||||||||
Authors | Moon-Walker, A. / Hastie, K.M. / Zyla, D.S. / Saphire, E.O. | ||||||||||||||||||||||||||||||
Funding support | United States, Switzerland, 9items
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Citation | Journal: Cell Chem Biol / Year: 2023 Title: Structural basis for antibody-mediated neutralization of lymphocytic choriomeningitis virus. Authors: Alex Moon-Walker / Zeli Zhang / Dawid S Zyla / Tierra K Buck / Haoyang Li / Ruben Diaz Avalos / Sharon L Schendel / Kathryn M Hastie / Shane Crotty / Erica Ollmann Saphire / Abstract: The mammarenavirus lymphocytic choriomeningitis virus (LCMV) is a globally distributed zoonotic pathogen that can be lethal in immunocompromised patients and can cause severe birth defects if ...The mammarenavirus lymphocytic choriomeningitis virus (LCMV) is a globally distributed zoonotic pathogen that can be lethal in immunocompromised patients and can cause severe birth defects if acquired during pregnancy. The structure of the trimeric surface glycoprotein, essential for entry, vaccine design, and antibody neutralization, remains unknown. Here, we present the cryoelectron microscopy (cryo-EM) structure of the LCMV surface glycoprotein (GP) in its trimeric pre-fusion assembly both alone and in complex with a rationally engineered monoclonal neutralizing antibody termed 18.5C-M28 (M28). Additionally, we show that passive administration of M28, either as a prophylactic or therapeutic, protects mice from LCMV clone 13 (LCMV) challenge. Our study illuminates not only the overall structural organization of LCMV GP and the mechanism for its inhibition by M28 but also presents a promising therapeutic candidate to prevent severe or fatal disease in individuals who are at risk of infection by a virus that poses a threat worldwide. | ||||||||||||||||||||||||||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 8dmi.cif.gz | 271.7 KB | Display | PDBx/mmCIF format |
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PDB format | pdb8dmi.ent.gz | 177.5 KB | Display | PDB format |
PDBx/mmJSON format | 8dmi.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 8dmi_validation.pdf.gz | 2.2 MB | Display | wwPDB validaton report |
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Full document | 8dmi_full_validation.pdf.gz | 2.2 MB | Display | |
Data in XML | 8dmi_validation.xml.gz | 38.9 KB | Display | |
Data in CIF | 8dmi_validation.cif.gz | 52.5 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/dm/8dmi ftp://data.pdbj.org/pub/pdb/validation_reports/dm/8dmi | HTTPS FTP |
-Related structure data
Related structure data | 27539MC 8dmhC M: map data used to model this data C: citing same article (ref.) |
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Similar structure data | Similarity search - Function & homologyF&H Search |
-Links
-Assembly
Deposited unit |
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Noncrystallographic symmetry (NCS) | NCS domain:
NCS domain segments:
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