National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)
GM122510
United States
Citation
Journal: Nat Commun / Year: 2023 Title: Archaeal DNA-import apparatus is homologous to bacterial conjugation machinery. Authors: Leticia C Beltran / Virginija Cvirkaite-Krupovic / Jessalyn Miller / Fengbin Wang / Mark A B Kreutzberger / Jonasz B Patkowski / Tiago R D Costa / Stefan Schouten / Ilya Levental / Vincent P ...Authors: Leticia C Beltran / Virginija Cvirkaite-Krupovic / Jessalyn Miller / Fengbin Wang / Mark A B Kreutzberger / Jonasz B Patkowski / Tiago R D Costa / Stefan Schouten / Ilya Levental / Vincent P Conticello / Edward H Egelman / Mart Krupovic / Abstract: Conjugation is a major mechanism of horizontal gene transfer promoting the spread of antibiotic resistance among human pathogens. It involves establishing a junction between a donor and a recipient ...Conjugation is a major mechanism of horizontal gene transfer promoting the spread of antibiotic resistance among human pathogens. It involves establishing a junction between a donor and a recipient cell via an extracellular appendage known as the mating pilus. In bacteria, the conjugation machinery is encoded by plasmids or transposons and typically mediates the transfer of cognate mobile genetic elements. Much less is known about conjugation in archaea. Here, we determine atomic structures by cryo-electron microscopy of three conjugative pili, two from hyperthermophilic archaea (Aeropyrum pernix and Pyrobaculum calidifontis) and one encoded by the Ti plasmid of the bacterium Agrobacterium tumefaciens, and show that the archaeal pili are homologous to bacterial mating pili. However, the archaeal conjugation machinery, known as Ced, has been 'domesticated', that is, the genes for the conjugation machinery are encoded on the chromosome rather than on mobile genetic elements, and mediates the transfer of cellular DNA.
History
Deposition
Jun 22, 2022
Deposition site: RCSB / Processing site: RCSB
Revision 1.0
Mar 22, 2023
Provider: repository / Type: Initial release
Revision 1.1
Jun 12, 2024
Group: Data collection / Category: chem_comp_atom / chem_comp_bond
B: Pilin protein A: Pilin protein C: Pilin protein D: Pilin protein E: Pilin protein F: Pilin protein G: Pilin protein H: Pilin protein I: Pilin protein J: Pilin protein K: Pilin protein L: Pilin protein M: Pilin protein N: Pilin protein O: Pilin protein P: Pilin protein Q: Pilin protein R: Pilin protein S: Pilin protein T: Pilin protein U: Pilin protein V: Pilin protein W: Pilin protein X: Pilin protein Y: Pilin protein Z: Pilin protein 0: Pilin protein 1: Pilin protein 2: Pilin protein 3: Pilin protein hetero molecules
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