ジャーナル: Proc Natl Acad Sci U S A / 年: 2023 タイトル: Yeast PIC-Mediator structure with RNA polymerase II C-terminal domain. 著者: Sandra Schilbach / Haibo Wang / Christian Dienemann / Patrick Cramer / 要旨: For transcription initiation, RNA polymerase II (Pol II) forms a preinitiation complex (PIC) that associates with the general coactivator Mediator. Whereas atomic models of the human PIC-Mediator ...For transcription initiation, RNA polymerase II (Pol II) forms a preinitiation complex (PIC) that associates with the general coactivator Mediator. Whereas atomic models of the human PIC-Mediator structure have been reported, structures for its yeast counterpart remain incomplete. Here, we present an atomic model for the yeast PIC with core Mediator, including the Mediator middle module that was previously poorly resolved and including subunit Med1 that was previously lacking. We observe three peptide regions containing eleven of the 26 heptapeptide repeats of the flexible C-terminal repeat domain (CTD) of Pol II. Two of these CTD regions bind between the Mediator head and middle modules and form defined CTD-Mediator interactions. CTD peptide 1 binds between the Med6 shoulder and Med31 knob domains, whereas CTD peptide 2 forms additional contacts with Med4. The third CTD region (peptide 3) binds in the Mediator cradle and associates with the Mediator hook. Comparisons with the human PIC-Mediator structure show that the central region in peptide 1 is similar and forms conserved contacts with Mediator, whereas peptides 2 and 3 exhibit distinct structures and Mediator interactions.
0: General transcription and DNA repair factor IIH helicase subunit XPD 1: General transcription and DNA repair factor IIH subunit TFB1 2: General transcription and DNA repair factor IIH subunit TFB2 3: RNA polymerase II transcription factor B subunit 3 4: General transcription and DNA repair factor IIH subunit TFB4 5: General transcription and DNA repair factor IIH subunit TFB5 6: General transcription and DNA repair factor IIH subunit SSL1 7: General transcription and DNA repair factor IIH helicase subunit XPB A: DNA-directed RNA polymerase II subunit RPB1 B: DNA-directed RNA polymerase II subunit RPB2 C: DNA-directed RNA polymerase II subunit RPB3 D: DNA-directed RNA polymerase II subunit RPB4 E: DNA-directed RNA polymerases I, II, and III subunit RPABC1 F: DNA-directed RNA polymerases I, II, and III subunit RPABC2 G: DNA-directed RNA polymerase II subunit RPB7 H: DNA-directed RNA polymerases I, II, and III subunit RPABC3 I: DNA-directed RNA polymerase II subunit RPB9 J: DNA-directed RNA polymerases I, II, and III subunit RPABC5 K: DNA-directed RNA polymerase II subunit RPB11 L: DNA-directed RNA polymerases I, II, and III subunit RPABC4 M: Transcription initiation factor IIB N: NONTEMPLATE DNA (209-MER) O: TATA-binding protein Q: Transcription initiation factor IIF subunit alpha R: Transcription initiation factor IIF subunit beta T: TEMPLATE DNA (209-MER) U: Transcription initiation factor IIA large subunit V: Transcription initiation factor IIA subunit 2 W: Transcription initiation factor IIE subunit alpha X: Transcription initiation factor IIE subunit beta a: Mediator of RNA polymerase II transcription subunit 6 b: Mediator of RNA polymerase II transcription subunit 8 c: Mediator of RNA polymerase II transcription subunit 11 d: Mediator of RNA polymerase II transcription subunit 17 e: Mediator of RNA polymerase II transcription subunit 18 f: Mediator of RNA polymerase II transcription subunit 20 g: Mediator of RNA polymerase II transcription subunit 22 h: Mediator of RNA polymerase II transcription subunit 4 i: Mediator of RNA polymerase II transcription subunit 7 j: Mediator of RNA polymerase II transcription subunit 9 k: Mediator of RNA polymerase II transcription subunit 10 l: Mediator of RNA polymerase II transcription subunit 14 m: Mediator of RNA polymerase II transcription subunit 19 n: Mediator of RNA polymerase II transcription subunit 21 o: Mediator of RNA polymerase II transcription subunit 31 p: Mediator of RNA polymerase II transcription subunit 1 ヘテロ分子
DNA-directed RNA polymerase II subunit ... , 7種, 7分子 ABCDGIK
#9: タンパク質
DNA-directedRNApolymeraseIIsubunitRPB1 / RNA polymerase II subunit B1 / DNA-directed RNA polymerase III largest subunit / RNA polymerase II ...RNA polymerase II subunit B1 / DNA-directed RNA polymerase III largest subunit / RNA polymerase II subunit B220
DNA-directed RNA polymerases I, II, and III subunit ... , 5種, 5分子 EFHJL
#13: タンパク質
DNA-directedRNApolymerasesI, II, andIIIsubunitRPABC1 / RNA polymerases I / II / and III subunit ABC1 / ABC27 / DNA-directed RNA polymerases I / and III 27 ...RNA polymerases I / II / and III subunit ABC1 / ABC27 / DNA-directed RNA polymerases I / and III 27 kDa polypeptide
DNA-directedRNApolymerasesI, II, andIIIsubunitRPABC2 / RNA polymerases I / II / and III subunit ABC2 / ABC23 / DNA-directed RNA polymerases I / and III 23 ...RNA polymerases I / II / and III subunit ABC2 / ABC23 / DNA-directed RNA polymerases I / and III 23 kDa polypeptide
DNA-directedRNApolymerasesI, II, andIIIsubunitRPABC3 / RNA polymerases I / II / and III subunit ABC3 / ABC14.4 / ABC14.5 / DNA-directed RNA polymerases I ...RNA polymerases I / II / and III subunit ABC3 / ABC14.4 / ABC14.5 / DNA-directed RNA polymerases I / and III 14.5 kDa polypeptide
DNA-directedRNApolymerasesI, II, andIIIsubunitRPABC5 / RNA polymerases I / II / and III subunit ABC5 / ABC10-beta / ABC8 / DNA-directed RNA polymerases I ...RNA polymerases I / II / and III subunit ABC5 / ABC10-beta / ABC8 / DNA-directed RNA polymerases I / and III 8.3 kDa polypeptide