+データを開く
-基本情報
登録情報 | データベース: PDB / ID: 7z5j | ||||||
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タイトル | The molybdenum storage protein loaded with tungstate | ||||||
要素 | (Molybdenum storage protein subunit ...) x 2 | ||||||
キーワード | METAL BINDING PROTEIN / Metal storage protein / polytungstate / Keggin ion / EM | ||||||
機能・相同性 | 機能・相同性情報 | ||||||
生物種 | Azotobacter vinelandii DJ (窒素固定) | ||||||
手法 | 電子顕微鏡法 / 単粒子再構成法 / クライオ電子顕微鏡法 / 解像度: 2.58 Å | ||||||
データ登録者 | Ermler, U. / Aziz, I. / Kaltwasser, S. / Kayastha, K. / Vonck, J. | ||||||
資金援助 | ドイツ, 1件
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引用 | ジャーナル: J Inorg Biochem / 年: 2022 タイトル: The molybdenum storage protein forms and deposits distinct polynuclear tungsten oxygen aggregates. 著者: Iram Aziz / Susann Kaltwasser / Kanwal Kayastha / Radhika Khera / Janet Vonck / Ulrich Ermler / 要旨: Some N-fixing bacteria store Mo to maintain the formation of the vital FeMo-cofactor dependent nitrogenase under Mo depleting conditions. The Mo storage protein (MoSto), developed for this purpose, ...Some N-fixing bacteria store Mo to maintain the formation of the vital FeMo-cofactor dependent nitrogenase under Mo depleting conditions. The Mo storage protein (MoSto), developed for this purpose, has the unique capability to compactly deposit molybdate as polyoxometalate (POM) clusters in a (αβ) hexameric cage; the same occurs with the physicochemically related tungstate. To explore the structural diversity of W-based POM clusters, MoSto loaded under different conditions with tungstate and two site-specifically modified MoSto variants were structurally characterized by X-ray crystallography or single-particle cryo-EM. The MoSto cage contains five major locations for POM clusters occupied among others by heptanuclear, Keggin ion and even Dawson-like species also found in bulk solvent under defined conditions. We found both lacunary derivatives of these archetypical POM clusters with missing WO units at positions exposed to bulk solvent and expanded derivatives with additional WO units next to protecting polypeptide segments or other POM clusters. The cryo-EM map, unexpectedly, reveals a POM cluster in the cage center anchored to the wall by a WO linker. Interestingly, distinct POM cluster structures can originate from identical, highly occupied core fragments of three to seven WO units that partly correspond to those found in MoSto loaded with molybdate. These core fragments are firmly bound to the complementary protein template in contrast to the more variable, less occupied residual parts of the visible POM clusters. Due to their higher stability, W-based POM clusters are, on average, larger and more diverse than their Mo-based counterparts. | ||||||
履歴 |
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-構造の表示
構造ビューア | 分子: MolmilJmol/JSmol |
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-ダウンロードとリンク
-ダウンロード
PDBx/mmCIF形式 | 7z5j.cif.gz | 749.4 KB | 表示 | PDBx/mmCIF形式 |
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PDB形式 | pdb7z5j.ent.gz | 580 KB | 表示 | PDB形式 |
PDBx/mmJSON形式 | 7z5j.json.gz | ツリー表示 | PDBx/mmJSON形式 | |
その他 | その他のダウンロード |
-検証レポート
文書・要旨 | 7z5j_validation.pdf.gz | 2.9 MB | 表示 | wwPDB検証レポート |
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文書・詳細版 | 7z5j_full_validation.pdf.gz | 3 MB | 表示 | |
XML形式データ | 7z5j_validation.xml.gz | 105.1 KB | 表示 | |
CIF形式データ | 7z5j_validation.cif.gz | 150.8 KB | 表示 | |
アーカイブディレクトリ | https://data.pdbj.org/pub/pdb/validation_reports/z5/7z5j ftp://data.pdbj.org/pub/pdb/validation_reports/z5/7z5j | HTTPS FTP |
-関連構造データ
-リンク
-集合体
登録構造単位 |
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-要素
-Molybdenum storage protein subunit ... , 2種, 12分子 BDFHJLACEIGK
#1: タンパク質 | 分子量: 28247.582 Da / 分子数: 6 / 由来タイプ: 組換発現 由来: (組換発現) Azotobacter vinelandii DJ (窒素固定) 遺伝子: mosB, Avin_43210 発現宿主: Escherichia coli 'BL21-Gold(DE3)pLysS AG' (大腸菌) 参照: UniProt: P84253 #2: タンパク質 | 分子量: 29245.582 Da / 分子数: 6 / 由来タイプ: 組換発現 由来: (組換発現) Azotobacter vinelandii DJ (窒素固定) 株: DJ / ATCC BAA-1303 / 遺伝子: mosA, Avin_43200 発現宿主: Escherichia coli 'BL21-Gold(DE3)pLysS AG' (大腸菌) 参照: UniProt: P84308 |
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-非ポリマー , 9種, 54分子
#3: 化合物 | ChemComp-MOO / #4: 化合物 | ChemComp-ATP / #5: 化合物 | ChemComp-MG / #6: 化合物 | ChemComp-IWL / #7: 化合物 | ChemComp-IV9 / |
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