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Open data
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Basic information
Entry | Database: PDB / ID: 7yo5 | ||||||
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Title | Cryo-EM structure of RCK1 mutated human Slo1 apo | ||||||
![]() | Calcium-activated potassium channel subunit alpha-1 | ||||||
![]() | TRANSPORT PROTEIN | ||||||
Function / homology | ![]() monoatomic ion-gated channel activity / ligand-gated monoatomic cation channel activity / potassium channel activity / membrane Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ELECTRON MICROSCOPY / single particle reconstruction / cryo EM / Resolution: 3.9 Å | ||||||
![]() | Yamanouchi, D. / Nureki, O. | ||||||
Funding support | ![]()
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![]() | ![]() Title: Structural basis for dual allosteric gating modulation of Slo1-LRRC channel complex Authors: Yamanouchi, D. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 620.6 KB | Display | ![]() |
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PDB format | ![]() | 501.6 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 1.5 MB | Display | ![]() |
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Full document | ![]() | 1.6 MB | Display | |
Data in XML | ![]() | 103.2 KB | Display | |
Data in CIF | ![]() | 153.4 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 33982MC ![]() 7ynzC ![]() 7yo0C ![]() 7yo1C ![]() 7yo2C ![]() 7yo3C ![]() 7yo4C M: map data used to model this data C: citing same article ( |
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Similar structure data | Similarity search - Function & homology ![]() |
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Links
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Assembly
Deposited unit | ![]()
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1 |
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Components
#1: Protein | Mass: 119370.484 Da / Num. of mol.: 4 / Mutation: D362A, D367A, K577S Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() #2: Chemical | ChemComp-CA / Has ligand of interest | Y | |
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-Experimental details
-Experiment
Experiment | Method: ELECTRON MICROSCOPY |
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EM experiment | Aggregation state: PARTICLE / 3D reconstruction method: single particle reconstruction |
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Sample preparation
Component | Name: human Slo1-LRRC26 complex / Type: COMPLEX / Entity ID: #1 / Source: RECOMBINANT |
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Molecular weight | Value: 616 kDa/nm / Experimental value: NO |
Source (natural) | Organism: ![]() |
Source (recombinant) | Organism: ![]() |
Buffer solution | pH: 8 |
Specimen | Conc.: 2 mg/ml / Embedding applied: NO / Shadowing applied: NO / Staining applied: NO / Vitrification applied: YES |
Vitrification | Cryogen name: ETHANE |
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Electron microscopy imaging
Experimental equipment | ![]() Model: Titan Krios / Image courtesy: FEI Company |
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Microscopy | Model: FEI TITAN KRIOS |
Electron gun | Electron source: ![]() |
Electron lens | Mode: BRIGHT FIELD / Nominal defocus max: 1600 nm / Nominal defocus min: 800 nm |
Image recording | Electron dose: 48 e/Å2 / Film or detector model: GATAN K3 (6k x 4k) |
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Processing
CTF correction | Type: NONE | ||||||||||||||||||||||||
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3D reconstruction | Resolution: 3.9 Å / Resolution method: FSC 0.143 CUT-OFF / Num. of particles: 149593 / Symmetry type: POINT | ||||||||||||||||||||||||
Refine LS restraints |
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