+Open data
-Basic information
Entry | Database: PDB / ID: 7y7k | ||||||
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Title | SARS-CoV-2 RBD in complex with 1F Fab | ||||||
Components |
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Keywords | VIRAL PROTEIN/IMMUNE SYSTEM / COVID-19 / spike glycoprotein / virus / VIRAL PROTEIN / VIRAL PROTEIN-IMMUNE SYSTEM complex | ||||||
Function / homology | Function and homology information Maturation of spike protein / viral translation / Translation of Structural Proteins / Virion Assembly and Release / host cell surface / host extracellular space / suppression by virus of host tetherin activity / Induction of Cell-Cell Fusion / structural constituent of virion / entry receptor-mediated virion attachment to host cell ...Maturation of spike protein / viral translation / Translation of Structural Proteins / Virion Assembly and Release / host cell surface / host extracellular space / suppression by virus of host tetherin activity / Induction of Cell-Cell Fusion / structural constituent of virion / entry receptor-mediated virion attachment to host cell / host cell endoplasmic reticulum-Golgi intermediate compartment membrane / receptor-mediated endocytosis of virus by host cell / membrane fusion / Attachment and Entry / positive regulation of viral entry into host cell / receptor-mediated virion attachment to host cell / receptor ligand activity / symbiont-mediated suppression of host innate immune response / host cell surface receptor binding / fusion of virus membrane with host plasma membrane / fusion of virus membrane with host endosome membrane / viral envelope / virion attachment to host cell / SARS-CoV-2 activates/modulates innate and adaptive immune responses / host cell plasma membrane / virion membrane / identical protein binding / membrane / plasma membrane Similarity search - Function | ||||||
Biological species | Severe acute respiratory syndrome coronavirus 2 Homo sapiens (human) | ||||||
Method | ELECTRON MICROSCOPY / single particle reconstruction / cryo EM / Resolution: 4.4 Å | ||||||
Authors | Zhao, S. / Liu, F. / Yang, X. / Zhong, G. | ||||||
Funding support | China, 1items
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Citation | Journal: Protein Cell / Year: 2023 Title: A core epitope targeting antibody of SARS-CoV-2. Authors: Simeng Zhao / Fengjiang Liu / Shizhen Qiu / Qiaoshuai Lan / Yiran Wu / Wei Xu / Junzi Ke / Jie Yang / Xiaoyan Liu / Kun Wang / Hangtian Guo / Shuai Xia / Fangfang Zhang / Jiabei Wang / ...Authors: Simeng Zhao / Fengjiang Liu / Shizhen Qiu / Qiaoshuai Lan / Yiran Wu / Wei Xu / Junzi Ke / Jie Yang / Xiaoyan Liu / Kun Wang / Hangtian Guo / Shuai Xia / Fangfang Zhang / Jiabei Wang / Xiaowen Hu / Lu Lu / Shibo Jiang / Suwen Zhao / Lianxin Liu / Youhua Xie / Xiuna Yang / Haopeng Wang / Guisheng Zhong / | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 7y7k.cif.gz | 78.5 KB | Display | PDBx/mmCIF format |
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PDB format | pdb7y7k.ent.gz | 58.3 KB | Display | PDB format |
PDBx/mmJSON format | 7y7k.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 7y7k_validation.pdf.gz | 1.2 MB | Display | wwPDB validaton report |
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Full document | 7y7k_full_validation.pdf.gz | 1.3 MB | Display | |
Data in XML | 7y7k_validation.xml.gz | 30.4 KB | Display | |
Data in CIF | 7y7k_validation.cif.gz | 41.5 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/y7/7y7k ftp://data.pdbj.org/pub/pdb/validation_reports/y7/7y7k | HTTPS FTP |
-Related structure data
Related structure data | 33668MC 7y7jC M: map data used to model this data C: citing same article (ref.) |
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Similar structure data | Similarity search - Function & homologyF&H Search |
-Links
-Assembly
Deposited unit |
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1 |
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-Components
#1: Protein | Mass: 20207.561 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Severe acute respiratory syndrome coronavirus 2 Gene: S, 2 / Production host: Homo sapiens (human) / References: UniProt: P0DTC2 |
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#2: Antibody | Mass: 14521.017 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Production host: Homo sapiens (human) |
#3: Antibody | Mass: 11719.901 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Production host: Homo sapiens (human) |
-Experimental details
-Experiment
Experiment | Method: ELECTRON MICROSCOPY |
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EM experiment | Aggregation state: PARTICLE / 3D reconstruction method: single particle reconstruction |
-Sample preparation
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Source (natural) |
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Source (recombinant) |
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Buffer solution | pH: 7.4 | ||||||||||||||||||||||||
Specimen | Embedding applied: NO / Shadowing applied: NO / Staining applied: NO / Vitrification applied: YES | ||||||||||||||||||||||||
Vitrification | Cryogen name: ETHANE |
-Electron microscopy imaging
Experimental equipment | Model: Titan Krios / Image courtesy: FEI Company |
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Microscopy | Model: FEI TITAN KRIOS |
Electron gun | Electron source: FIELD EMISSION GUN / Accelerating voltage: 300 kV / Illumination mode: SPOT SCAN |
Electron lens | Mode: BRIGHT FIELD / Nominal defocus max: 2400 nm / Nominal defocus min: 1000 nm |
Image recording | Electron dose: 60 e/Å2 / Film or detector model: GATAN K3 (6k x 4k) |
-Processing
Software | Name: PHENIX / Version: 1.16_3549: / Classification: refinement | ||||||||||||||||||||||||
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CTF correction | Type: PHASE FLIPPING AND AMPLITUDE CORRECTION | ||||||||||||||||||||||||
3D reconstruction | Resolution: 4.4 Å / Resolution method: FSC 0.143 CUT-OFF / Num. of particles: 61103 / Symmetry type: POINT | ||||||||||||||||||||||||
Refinement | Highest resolution: 4.4 Å | ||||||||||||||||||||||||
Refine LS restraints |
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