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- PDB-7y36: Cryo-EM structure of the Teriparatide-bound human PTH1R-Gs complex -
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Open data
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Basic information
Entry | Database: PDB / ID: 7y36 | ||||||
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Title | Cryo-EM structure of the Teriparatide-bound human PTH1R-Gs complex | ||||||
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Function / homology | ![]() type 1 parathyroid hormone receptor binding / ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() Similarity search - Function | ||||||
Biological species | ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() synthetic construct (others) | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Zhai, X. / Mao, C. / Shen, Q. / Zang, S. / Shen, D. / Zhang, H. / Chen, Z. / Wang, G. / Zhang, C. / Zhang, Y. / Liu, Z. | ||||||
Funding support | 1items
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![]() | ![]() Title: Cryo-EM structure of the Teriparatide-bound human PTH1R-Gs complex Authors: Zhai, X. / Mao, C. / Shen, Q. / Zang, S. / Shen, D. / Zhang, H. / Chen, Z. / Wang, G. / Zhang, C. / Zhang, Y. / Liu, Z. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 224.2 KB | Display | ![]() |
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PDB format | ![]() | 172.3 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Related structure data | ![]() 33590MC M: map data used to model this data C: citing same article ( |
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Similar structure data | Similarity search - Function & homology ![]() |
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Links
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Assembly
Deposited unit | ![]()
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1 |
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Components
-Protein , 2 types, 2 molecules RA
#1: Protein | Mass: 81730.609 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() ![]() |
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#3: Protein | Mass: 44257.051 Da / Num. of mol.: 1 / Fragment: G226A,E268A,N271K,K274D,R280K,T284D,I285T / Mutation: G226A,E268A,N271K,K274D,R280K,T284D,I285T Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() ![]() |
-Guanine nucleotide-binding protein ... , 2 types, 2 molecules BG
#4: Protein | Mass: 41441.320 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() ![]() ![]() |
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#5: Protein | Mass: 7729.947 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() ![]() ![]() |
-Protein/peptide / Antibody / Non-polymers , 3 types, 3 molecules PN![](data/chem/img/OH.gif)
![](data/chem/img/OH.gif)
#2: Protein/peptide | ![]() Mass: 4125.778 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) ![]() ![]() |
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#6: Antibody | ![]() Mass: 13711.284 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) synthetic construct (others) / Production host: ![]() ![]() ![]() |
#7: Chemical | ChemComp-OH / ![]() |
-Details
Has ligand of interest | Y |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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EM experiment | Aggregation state: PARTICLE / 3D reconstruction method: ![]() |
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Sample preparation
Component | Name: Cryo-EM structure of the Teriparatide-bound human PTH1R-Gs complex Type: COMPLEX / Entity ID: #1-#6 / Source: MULTIPLE SOURCES |
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Source (natural) | Organism: ![]() ![]() |
Source (recombinant) | Organism: ![]() ![]() ![]() |
Buffer solution | pH: 7.4 |
Specimen | Embedding applied: NO / Shadowing applied: NO / Staining applied![]() ![]() |
Specimen support | Grid type: Quantifoil R1.2/1.3 |
Vitrification![]() | Cryogen name: ETHANE / Humidity: 100 % / Chamber temperature: 277 K |
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Electron microscopy imaging
Experimental equipment | ![]() Model: Titan Krios / Image courtesy: FEI Company |
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Microscopy | Model: FEI TITAN KRIOS |
Electron gun | Electron source![]() ![]() |
Electron lens | Mode: BRIGHT FIELD![]() |
Specimen holder | Cryogen: NITROGEN / Specimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER |
Image recording | Electron dose: 64 e/Å2 / Film or detector model: GATAN K2 SUMMIT (4k x 4k) / Num. of real images: 6947 |
Image scans | Movie frames/image: 40 / Used frames/image: 1-40 |
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Processing
CTF correction![]() | Type: PHASE FLIPPING AND AMPLITUDE CORRECTION | ||||||||||||||||||||||||
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Particle selection | Num. of particles selected: 3607078 | ||||||||||||||||||||||||
3D reconstruction![]() | Resolution: 2.8 Å / Resolution method: FSC 0.143 CUT-OFF / Num. of particles: 922971 / Symmetry type: POINT | ||||||||||||||||||||||||
Refine LS restraints |
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