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Open data
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Basic information
Entry | Database: PDB / ID: 7xs7 | ||||||
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Title | structure of a membrane-integrated glycosyltransferase | ||||||
![]() | Chitin synthase 1 | ||||||
![]() | MEMBRANE PROTEIN / glycosyltransferase / TRANSFERASE | ||||||
Function / homology | ![]() chitosome / chitin synthase / chitin synthase activity / : / septum digestion after cytokinesis / cell septum / cell periphery / cell wall organization / plasma membrane Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ELECTRON MICROSCOPY / single particle reconstruction / cryo EM / Resolution: 3.2 Å | ||||||
![]() | Wu, Y.N. / Zhang, M. / Yang, Y.Z. / Ding, X.Y. / Liu, X.T. / Zhang, M.J. / Yu, H.J. | ||||||
Funding support | ![]()
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![]() | ![]() Title: structure of a membrane-integrated glycosyltransferase with inhibitor Authors: Wu, Y.N. / Zhang, M. / Yang, Y.Z. / Ding, X.Y. / Liu, X.T. / Zhang, M.J. / Yu, H.J. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 292.2 KB | Display | ![]() |
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PDB format | ![]() | 227.6 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Related structure data | ![]() 33423MC ![]() 7xs6C M: map data used to model this data C: citing same article ( |
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Similar structure data | Similarity search - Function & homology ![]() |
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Links
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Assembly
Deposited unit | ![]()
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1 |
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Components
#1: Protein | Mass: 130001.141 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() Strain: ATCC 204508 / S288c / Gene: CHS1, YNL192W, N1404 / Production host: ![]() #2: Chemical | ChemComp-C14 / #3: Chemical | ChemComp-D12 / #4: Chemical | ChemComp-HP6 / #5: Chemical | Has ligand of interest | N | |
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-Experimental details
-Experiment
Experiment | Method: ELECTRON MICROSCOPY |
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EM experiment | Aggregation state: PARTICLE / 3D reconstruction method: single particle reconstruction |
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Sample preparation
Component | Name: membrane-integrated glycosyltransferase / Type: COMPLEX / Entity ID: #1 / Source: RECOMBINANT |
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Source (natural) | Organism: ![]() ![]() |
Source (recombinant) | Organism: ![]() |
Buffer solution | pH: 7.4 |
Specimen | Embedding applied: NO / Shadowing applied: NO / Staining applied: NO / Vitrification applied: YES |
Vitrification | Cryogen name: ETHANE |
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Electron microscopy imaging
Experimental equipment | ![]() Model: Titan Krios / Image courtesy: FEI Company |
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Microscopy | Model: FEI TITAN KRIOS |
Electron gun | Electron source: ![]() |
Electron lens | Mode: BRIGHT FIELD / Nominal defocus max: 3000 nm / Nominal defocus min: 1000 nm |
Image recording | Electron dose: 50 e/Å2 / Film or detector model: GATAN K3 (6k x 4k) |
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Processing
Software | Name: PHENIX / Version: 1.19.1_4122: / Classification: refinement | ||||||||||||||||||||||||
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CTF correction | Type: PHASE FLIPPING ONLY | ||||||||||||||||||||||||
3D reconstruction | Resolution: 3.2 Å / Resolution method: FSC 0.143 CUT-OFF / Num. of particles: 93641 / Symmetry type: POINT | ||||||||||||||||||||||||
Refine LS restraints |
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