+Open data
-Basic information
Entry | Database: PDB / ID: 7xlm | ||||||
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Title | Human diastrophic dysplasia sulfate transporter SLC26A2 | ||||||
Components | Sulfate transporter | ||||||
Keywords | TRANSPORT PROTEIN / Transporter / sulfate | ||||||
Function / homology | Function and homology information Defective SLC26A2 causes chondrodysplasias / sulfate transmembrane transport / Multifunctional anion exchangers / Transport and synthesis of PAPS / sulfate transport / sulfate transmembrane transporter activity / chondrocyte proliferation / secondary active sulfate transmembrane transporter activity / oxalate transmembrane transporter activity / solute:inorganic anion antiporter activity ...Defective SLC26A2 causes chondrodysplasias / sulfate transmembrane transport / Multifunctional anion exchangers / Transport and synthesis of PAPS / sulfate transport / sulfate transmembrane transporter activity / chondrocyte proliferation / secondary active sulfate transmembrane transporter activity / oxalate transmembrane transporter activity / solute:inorganic anion antiporter activity / bicarbonate transmembrane transporter activity / chloride transmembrane transporter activity / microvillus membrane / chondrocyte differentiation / ossification / apical plasma membrane / extracellular exosome / membrane / plasma membrane Similarity search - Function | ||||||
Biological species | Homo sapiens (human) | ||||||
Method | ELECTRON MICROSCOPY / single particle reconstruction / cryo EM / Resolution: 3.73 Å | ||||||
Authors | Chi, X.M. / Zhou, Q. | ||||||
Funding support | China, 1items
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Citation | Journal: Dian Zi Xian Wei Xue Bao / Year: 2023 Title: Structure of human diastrophic dysplasia sulfate transporter SLC26A2 Authors: Chi, X.M. / Li, X.R. / Huang, B.D. / Zhou, Q. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 7xlm.cif.gz | 217.9 KB | Display | PDBx/mmCIF format |
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PDB format | pdb7xlm.ent.gz | 170.8 KB | Display | PDB format |
PDBx/mmJSON format | 7xlm.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 7xlm_validation.pdf.gz | 1.1 MB | Display | wwPDB validaton report |
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Full document | 7xlm_full_validation.pdf.gz | 1.1 MB | Display | |
Data in XML | 7xlm_validation.xml.gz | 40.5 KB | Display | |
Data in CIF | 7xlm_validation.cif.gz | 60.6 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/xl/7xlm ftp://data.pdbj.org/pub/pdb/validation_reports/xl/7xlm | HTTPS FTP |
-Related structure data
Related structure data | 33284MC M: map data used to model this data C: citing same article (ref.) |
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Similar structure data | Similarity search - Function & homologyF&H Search |
-Links
-Assembly
Deposited unit |
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1 |
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-Components
#1: Protein | Mass: 88273.945 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Gene: SLC26A2, DTD, DTDST / Production host: Homo sapiens (human) / References: UniProt: P50443 #2: Chemical | Has ligand of interest | Y | |
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-Experimental details
-Experiment
Experiment | Method: ELECTRON MICROSCOPY |
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EM experiment | Aggregation state: PARTICLE / 3D reconstruction method: single particle reconstruction |
-Sample preparation
Component | Name: human diastrophic dysplasia sulfate transporter SLC26A2 Type: COMPLEX / Entity ID: #1 / Source: RECOMBINANT |
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Molecular weight | Value: 0.13 MDa / Experimental value: NO |
Source (natural) | Organism: Homo sapiens (human) |
Source (recombinant) | Organism: Homo sapiens (human) |
Buffer solution | pH: 7.4 |
Specimen | Embedding applied: NO / Shadowing applied: NO / Staining applied: NO / Vitrification applied: YES |
Specimen support | Grid material: GOLD / Grid type: Quantifoil R1.2/1.3 |
Vitrification | Cryogen name: ETHANE |
-Electron microscopy imaging
Experimental equipment | Model: Titan Krios / Image courtesy: FEI Company |
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Microscopy | Model: FEI TITAN KRIOS |
Electron gun | Electron source: FIELD EMISSION GUN / Accelerating voltage: 300 kV / Illumination mode: SPOT SCAN |
Electron lens | Mode: BRIGHT FIELD / Nominal defocus max: 2300 nm / Nominal defocus min: 1500 nm |
Image recording | Electron dose: 50 e/Å2 / Film or detector model: GATAN K3 BIOQUANTUM (6k x 4k) |
-Processing
Software | Name: PHENIX / Version: 1.14_3260: / Classification: refinement |
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CTF correction | Type: PHASE FLIPPING AND AMPLITUDE CORRECTION |
3D reconstruction | Resolution: 3.73 Å / Resolution method: FSC 0.143 CUT-OFF / Num. of particles: 395589 / Symmetry type: POINT |