+Open data
-Basic information
Entry | Database: PDB / ID: 7wm1 | ||||||
---|---|---|---|---|---|---|---|
Title | Cryo-EM structure of AKT1-AtKC1 | ||||||
Components |
| ||||||
Keywords | MEMBRANE PROTEIN / VGIC / Plant Channel | ||||||
Function / homology | Function and homology information root hair elongation / regulation of stomatal closure / response to nematode / response to water deprivation / inward rectifier potassium channel activity / monoatomic ion channel complex / voltage-gated potassium channel activity / potassium ion import across plasma membrane / potassium ion transmembrane transport / response to salt stress ...root hair elongation / regulation of stomatal closure / response to nematode / response to water deprivation / inward rectifier potassium channel activity / monoatomic ion channel complex / voltage-gated potassium channel activity / potassium ion import across plasma membrane / potassium ion transmembrane transport / response to salt stress / potassium ion transport / endoplasmic reticulum / identical protein binding / plasma membrane Similarity search - Function | ||||||
Biological species | Arabidopsis thaliana (thale cress) | ||||||
Method | ELECTRON MICROSCOPY / single particle reconstruction / cryo EM / Resolution: 2.8 Å | ||||||
Authors | Dongliang, L. / Zijie, Z. / Yannan, Q. / Yuyue, T. / Huaizong, S. | ||||||
Funding support | China, 1items
| ||||||
Citation | Journal: To Be Published Title: Structural basis for the regulation mechanism of a hyper polarization-activated K+ channel AKT1 by AtKC1 Authors: Dongliang, L. / Zijie, Z. / Yannan, Q. / Yuyue, T. / Huaizong, S. | ||||||
History |
|
-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
---|
-Downloads & links
-Download
PDBx/mmCIF format | 7wm1.cif.gz | 364.4 KB | Display | PDBx/mmCIF format |
---|---|---|---|---|
PDB format | pdb7wm1.ent.gz | 281 KB | Display | PDB format |
PDBx/mmJSON format | 7wm1.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/wm/7wm1 ftp://data.pdbj.org/pub/pdb/validation_reports/wm/7wm1 | HTTPS FTP |
---|
-Related structure data
Related structure data | 32597MC M: map data used to model this data C: citing same article (ref.) |
---|---|
Similar structure data | Similarity search - Function & homologyF&H Search |
-Links
-Assembly
Deposited unit |
|
---|---|
1 |
|
-Components
#1: Protein | Mass: 99812.266 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Arabidopsis thaliana (thale cress) / Gene: AKT1, At2g26650, F18A8.2 / Production host: Homo sapiens (human) / References: UniProt: Q38998 #2: Protein | Mass: 78435.859 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Arabidopsis thaliana (thale cress) / Gene: KAT3, AKT4, KC1, At4g32650, F4D11.150 / Production host: Homo sapiens (human) / References: UniProt: P92960 #3: Chemical | ChemComp-POV / ( #4: Chemical | Has ligand of interest | N | |
---|
-Experimental details
-Experiment
Experiment | Method: ELECTRON MICROSCOPY |
---|---|
EM experiment | Aggregation state: PARTICLE / 3D reconstruction method: single particle reconstruction |
-Sample preparation
Component | Name: Complex of AKT1 and AtKC1 / Type: COMPLEX / Entity ID: #1-#2 / Source: RECOMBINANT |
---|---|
Source (natural) | Organism: Arabidopsis thaliana (thale cress) |
Source (recombinant) | Organism: Homo sapiens (human) |
Buffer solution | pH: 8 |
Specimen | Embedding applied: NO / Shadowing applied: NO / Staining applied: NO / Vitrification applied: YES |
Vitrification | Cryogen name: ETHANE |
-Electron microscopy imaging
Experimental equipment | Model: Titan Krios / Image courtesy: FEI Company |
---|---|
Microscopy | Model: FEI TITAN KRIOS |
Electron gun | Electron source: FIELD EMISSION GUN / Accelerating voltage: 300 kV / Illumination mode: FLOOD BEAM |
Electron lens | Mode: BRIGHT FIELD / Nominal defocus max: 1800 nm / Nominal defocus min: 1200 nm |
Image recording | Electron dose: 50 e/Å2 / Film or detector model: GATAN K3 (6k x 4k) |
-Processing
Software | Name: PHENIX / Version: 1.17.1_3660: / Classification: refinement | ||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
CTF correction | Type: PHASE FLIPPING AND AMPLITUDE CORRECTION | ||||||||||||||||||||||||
3D reconstruction | Resolution: 2.8 Å / Resolution method: FSC 0.143 CUT-OFF / Num. of particles: 289326 / Symmetry type: POINT | ||||||||||||||||||||||||
Refine LS restraints |
|