[English] 日本語
Yorodumi- PDB-7wch: CryoEM structure of the SARS-CoV-2 S6P(B.1.617.2) in complex with... -
+Open data
-Basic information
Entry | Database: PDB / ID: 7wch | ||||||
---|---|---|---|---|---|---|---|
Title | CryoEM structure of the SARS-CoV-2 S6P(B.1.617.2) in complex with SWA9 Fab | ||||||
Components |
| ||||||
Keywords | VIRAL PROTEIN / Complex / antibody | ||||||
Function / homology | : Function and homology information | ||||||
Biological species | Severe acute respiratory syndrome coronavirus 2 Homo sapiens (human) | ||||||
Method | ELECTRON MICROSCOPY / single particle reconstruction / cryo EM / Resolution: 3.19 Å | ||||||
Authors | Du, S. / Xiao, J.Y. | ||||||
Funding support | 1items
| ||||||
Citation | Journal: To Be Published Title: CryoEM structure of the SARS-CoV-2 S6P(B.1.617.2) in complex with SWA9 Fab Authors: Du, S. / Xiao, J.Y. | ||||||
History |
|
-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
---|
-Downloads & links
-Download
PDBx/mmCIF format | 7wch.cif.gz | 647.4 KB | Display | PDBx/mmCIF format |
---|---|---|---|---|
PDB format | pdb7wch.ent.gz | 520.9 KB | Display | PDB format |
PDBx/mmJSON format | 7wch.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 7wch_validation.pdf.gz | 908.8 KB | Display | wwPDB validaton report |
---|---|---|---|---|
Full document | 7wch_full_validation.pdf.gz | 962 KB | Display | |
Data in XML | 7wch_validation.xml.gz | 98.1 KB | Display | |
Data in CIF | 7wch_validation.cif.gz | 148.4 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/wc/7wch ftp://data.pdbj.org/pub/pdb/validation_reports/wc/7wch | HTTPS FTP |
-Related structure data
Related structure data | 32422MC M: map data used to model this data C: citing same article (ref.) |
---|---|
Similar structure data | Similarity search - Function & homologyF&H Search |
-Links
-Assembly
Deposited unit |
|
---|---|
1 |
|
-Components
#1: Protein | Mass: 142238.312 Da / Num. of mol.: 3 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Severe acute respiratory syndrome coronavirus 2 Production host: Homo sapiens (human) / References: UniProt: A0A8B1VBI1 #2: Antibody | Mass: 24304.322 Da / Num. of mol.: 3 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Production host: Homo sapiens (human) #3: Antibody | Mass: 12182.615 Da / Num. of mol.: 3 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Production host: Homo sapiens (human) Has ligand of interest | N | |
---|
-Experimental details
-Experiment
Experiment | Method: ELECTRON MICROSCOPY |
---|---|
EM experiment | Aggregation state: PARTICLE / 3D reconstruction method: single particle reconstruction |
-Sample preparation
Component | Name: S6P(B.1.617.2) in complex with SWA9 / Type: COMPLEX / Entity ID: all / Source: RECOMBINANT |
---|---|
Source (natural) | Organism: Homo sapiens (human) |
Source (recombinant) | Organism: Homo sapiens (human) |
Buffer solution | pH: 7.2 |
Specimen | Embedding applied: NO / Shadowing applied: NO / Staining applied: NO / Vitrification applied: YES |
Vitrification | Cryogen name: ETHANE |
-Electron microscopy imaging
Experimental equipment | Model: Titan Krios / Image courtesy: FEI Company |
---|---|
Microscopy | Model: FEI TITAN KRIOS |
Electron gun | Electron source: FIELD EMISSION GUN / Accelerating voltage: 300 kV / Illumination mode: FLOOD BEAM |
Electron lens | Mode: DARK FIELD / Nominal defocus max: 1500 nm / Nominal defocus min: 1000 nm |
Image recording | Electron dose: 50 e/Å2 / Film or detector model: GATAN K3 (6k x 4k) |
-Processing
CTF correction | Type: PHASE FLIPPING AND AMPLITUDE CORRECTION |
---|---|
3D reconstruction | Resolution: 3.19 Å / Resolution method: FSC 0.143 CUT-OFF / Num. of particles: 324843 / Symmetry type: POINT |