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Yorodumi- PDB-7w7g: Structure of Mammalian NALCN-FAM155A-UNC79-UNC80 quanternary complex -
+Open data
-Basic information
Entry | Database: PDB / ID: 7w7g | |||||||||||||||
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Title | Structure of Mammalian NALCN-FAM155A-UNC79-UNC80 quanternary complex | |||||||||||||||
Components |
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Keywords | MEMBRANE PROTEIN / Sodium leak channel / NALCN / channel / FAM155 / UNC79 / UNC80 | |||||||||||||||
Function / homology | Function and homology information monoatomic cation homeostasis / positive regulation of synaptic transmission, cholinergic / leak channel activity / regulation of resting membrane potential / cation channel complex / voltage-gated monoatomic ion channel activity / Stimuli-sensing channels / regulation of monoatomic ion transmembrane transport / voltage-gated sodium channel activity / calcium ion import across plasma membrane ...monoatomic cation homeostasis / positive regulation of synaptic transmission, cholinergic / leak channel activity / regulation of resting membrane potential / cation channel complex / voltage-gated monoatomic ion channel activity / Stimuli-sensing channels / regulation of monoatomic ion transmembrane transport / voltage-gated sodium channel activity / calcium ion import across plasma membrane / sodium ion transmembrane transport / monoatomic cation channel activity / potassium ion transmembrane transport / positive regulation of synaptic transmission, GABAergic / calcium ion transmembrane transport / axon / plasma membrane Similarity search - Function | |||||||||||||||
Biological species | Mus musculus (house mouse) Rattus norvegicus (Norway rat) | |||||||||||||||
Method | ELECTRON MICROSCOPY / single particle reconstruction / cryo EM / Resolution: 3.2 Å | |||||||||||||||
Authors | Chen, L. / Kang, Y. | |||||||||||||||
Funding support | China, 4items
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Citation | Journal: Nat Commun / Year: 2022 Title: Structure and mechanism of NALCN-FAM155A-UNC79-UNC80 channel complex. Authors: Yunlu Kang / Lei Chen / Abstract: NALCN channel mediates sodium leak currents and is important for maintaining proper resting membrane potential. NALCN and FAM155A form the core complex of the channel, the activity of which ...NALCN channel mediates sodium leak currents and is important for maintaining proper resting membrane potential. NALCN and FAM155A form the core complex of the channel, the activity of which essentially depends on the presence of both UNC79 and UNC80, two auxiliary proteins. NALCN, FAM155A, UNC79, and UNC80 co-assemble into a large hetero-tetrameric channel complex. Genetic mutations of NALCN channel components lead to neurodevelopmental diseases. However, the structure and mechanism of the intact channel complex remain elusive. Here, we present the cryo-EM structure of the mammalian NALCN-FAM155A-UNC79-UNC80 quaternary complex. The structure shows that UNC79-UNC80 form a large piler-shaped heterodimer which was tethered to the intracellular side of the NALCN channel through tripartite interactions with the cytoplasmic loops of NALCN. Two interactions are essential for proper cell surface localization of NALCN. The other interaction relieves the self-inhibition of NALCN by pulling the auto-inhibitory CTD Interacting Helix (CIH) out of its binding site. Our work defines the structural mechanism of NALCN modulation by UNC79 and UNC80. | |||||||||||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 7w7g.cif.gz | 898.7 KB | Display | PDBx/mmCIF format |
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PDB format | pdb7w7g.ent.gz | 697.3 KB | Display | PDB format |
PDBx/mmJSON format | 7w7g.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 7w7g_validation.pdf.gz | 977.9 KB | Display | wwPDB validaton report |
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Full document | 7w7g_full_validation.pdf.gz | 1 MB | Display | |
Data in XML | 7w7g_validation.xml.gz | 115.2 KB | Display | |
Data in CIF | 7w7g_validation.cif.gz | 177.7 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/w7/7w7g ftp://data.pdbj.org/pub/pdb/validation_reports/w7/7w7g | HTTPS FTP |
-Related structure data
Related structure data | 32344MC M: map data used to model this data C: citing same article (ref.) |
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Similar structure data | Similarity search - Function & homologyF&H Search |
-Links
-Assembly
Deposited unit |
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1 |
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-Components
-Protein , 4 types, 4 molecules ABCD
#1: Protein | Mass: 297597.562 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Mus musculus (house mouse) / Gene: Unc79 / Production host: Homo sapiens (human) / References: UniProt: E5CYJ9 |
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#2: Protein | Mass: 371044.062 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Mus musculus (house mouse) / Production host: Homo sapiens (human) / References: UniProt: B8XCJ6 |
#3: Protein | Mass: 200682.031 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Rattus norvegicus (Norway rat) / Gene: Nalcn, Nca, Rb21, Vgcnl1 / Production host: Homo sapiens (human) / References: UniProt: Q6Q760 |
#4: Protein | Mass: 52795.801 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Mus musculus (house mouse) / Gene: Fam155a / Production host: Homo sapiens (human) / References: UniProt: Q8CCS2 |
-Non-polymers / Sugars , 2 types, 5 molecules
#5: Chemical | #6: Sugar | |
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-Details
Has ligand of interest | Y |
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-Experimental details
-Experiment
Experiment | Method: ELECTRON MICROSCOPY |
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EM experiment | Aggregation state: PARTICLE / 3D reconstruction method: single particle reconstruction |
-Sample preparation
Component | Name: NALCN-FAM155A-UNC79-UNC80 complex / Type: COMPLEX / Entity ID: #1-#4 / Source: RECOMBINANT |
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Source (natural) | Organism: Mus musculus (house mouse) |
Source (recombinant) | Organism: Homo sapiens (human) |
Buffer solution | pH: 7.5 |
Specimen | Embedding applied: NO / Shadowing applied: NO / Staining applied: NO / Vitrification applied: YES |
Vitrification | Cryogen name: ETHANE |
-Electron microscopy imaging
Experimental equipment | Model: Titan Krios / Image courtesy: FEI Company |
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Microscopy | Model: FEI TITAN KRIOS |
Electron gun | Electron source: FIELD EMISSION GUN / Accelerating voltage: 300 kV / Illumination mode: FLOOD BEAM |
Electron lens | Mode: BRIGHT FIELD / Nominal defocus max: 1800 nm / Nominal defocus min: 1500 nm |
Image recording | Electron dose: 50 e/Å2 / Film or detector model: GATAN K2 QUANTUM (4k x 4k) |
-Processing
CTF correction | Type: NONE |
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3D reconstruction | Resolution: 3.2 Å / Resolution method: FSC 0.143 CUT-OFF / Num. of particles: 275170 / Symmetry type: POINT |