+Open data
-Basic information
Entry | Database: PDB / ID: 7umn | |||||||||
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Title | H1 Solomon Islands 2006 hemagglutinin in complex with Ab36 | |||||||||
Components |
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Keywords | VIRAL PROTEIN / influenza / antibody / vaccine | |||||||||
Function / homology | Function and homology information viral budding from plasma membrane / clathrin-dependent endocytosis of virus by host cell / host cell surface receptor binding / apical plasma membrane / fusion of virus membrane with host plasma membrane / fusion of virus membrane with host endosome membrane / viral envelope / virion attachment to host cell / host cell plasma membrane / virion membrane Similarity search - Function | |||||||||
Biological species | Influenza A virus Mus musculus (house mouse) | |||||||||
Method | ELECTRON MICROSCOPY / single particle reconstruction / cryo EM / Resolution: 3.6 Å | |||||||||
Authors | Windsor, I.W. / Caradonna, T.M. / Schmidt, A.G. | |||||||||
Funding support | United States, 2items
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Citation | Journal: To Be Published Title: H1 Solomon Islands 2006 hemagglutinin in complex with Ab36 Authors: Carradona, T. | |||||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 7umn.cif.gz | 293.3 KB | Display | PDBx/mmCIF format |
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PDB format | pdb7umn.ent.gz | 230.7 KB | Display | PDB format |
PDBx/mmJSON format | 7umn.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 7umn_validation.pdf.gz | 992.3 KB | Display | wwPDB validaton report |
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Full document | 7umn_full_validation.pdf.gz | 1016.8 KB | Display | |
Data in XML | 7umn_validation.xml.gz | 49.4 KB | Display | |
Data in CIF | 7umn_validation.cif.gz | 73.5 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/um/7umn ftp://data.pdbj.org/pub/pdb/validation_reports/um/7umn | HTTPS FTP |
-Related structure data
Related structure data | 26606MC M: map data used to model this data C: citing same article (ref.) |
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Similar structure data | Similarity search - Function & homologyF&H Search |
-Links
-Assembly
Deposited unit |
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1 |
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-Components
#1: Protein | Mass: 58608.316 Da / Num. of mol.: 3 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Influenza A virus (A/Solomon Islands/3/2006(H1N1)) Strain: A/Solomon Islands/3/2006(H1N1) / Gene: HA / Production host: Homo sapiens (human) / References: UniProt: A7Y8I1 #2: Antibody | Mass: 25284.295 Da / Num. of mol.: 3 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Mus musculus (house mouse) / Production host: Homo sapiens (human) #3: Antibody | Mass: 23375.693 Da / Num. of mol.: 3 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Mus musculus (house mouse) / Production host: Homo sapiens (human) |
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-Experimental details
-Experiment
Experiment | Method: ELECTRON MICROSCOPY |
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EM experiment | Aggregation state: PARTICLE / 3D reconstruction method: single particle reconstruction |
-Sample preparation
Component |
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Source (natural) |
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Source (recombinant) |
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Buffer solution | pH: 7.5 | ||||||||||||||||||||||||
Specimen | Embedding applied: NO / Shadowing applied: NO / Staining applied: NO / Vitrification applied: YES | ||||||||||||||||||||||||
Vitrification | Cryogen name: ETHANE |
-Electron microscopy imaging
Experimental equipment | Model: Titan Krios / Image courtesy: FEI Company |
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Microscopy | Model: FEI TITAN KRIOS |
Electron gun | Electron source: FIELD EMISSION GUN / Accelerating voltage: 300 kV / Illumination mode: FLOOD BEAM |
Electron lens | Mode: BRIGHT FIELD |
Image recording | Electron dose: 1.07 e/Å2 / Film or detector model: GATAN K3 BIOQUANTUM (6k x 4k) |
-Processing
Software | Name: PHENIX / Version: 1.18.2_3874: / Classification: refinement | ||||||||||||||||||||||||
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EM software |
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CTF correction | Type: NONE | ||||||||||||||||||||||||
3D reconstruction | Resolution: 3.6 Å / Resolution method: FSC 0.143 CUT-OFF / Num. of particles: 124426 / Symmetry type: POINT | ||||||||||||||||||||||||
Refine LS restraints |
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