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-基本情報
登録情報 | データベース: PDB / ID: 7sfk | ||||||
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タイトル | ChRmine in MSP1E3D1 lipid nanodisc | ||||||
要素 | ChRmine | ||||||
キーワード | MEMBRANE PROTEIN / Channelrhodopsin / ion channel | ||||||
機能・相同性 | 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine / RETINAL 機能・相同性情報 | ||||||
生物種 | Rhodomonas lens (真核生物) | ||||||
手法 | 電子顕微鏡法 / 単粒子再構成法 / クライオ電子顕微鏡法 / 解像度: 2.74 Å | ||||||
データ登録者 | Tucker, K. / Brohawn, S. | ||||||
資金援助 | 米国, 1件
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引用 | ジャーナル: Nat Commun / 年: 2022 タイトル: Cryo-EM structures of the channelrhodopsin ChRmine in lipid nanodiscs. 著者: Kyle Tucker / Savitha Sridharan / Hillel Adesnik / Stephen G Brohawn / 要旨: Microbial channelrhodopsins are light-gated ion channels widely used for optogenetic manipulation of neuronal activity. ChRmine is a bacteriorhodopsin-like cation channelrhodopsin (BCCR) more closely ...Microbial channelrhodopsins are light-gated ion channels widely used for optogenetic manipulation of neuronal activity. ChRmine is a bacteriorhodopsin-like cation channelrhodopsin (BCCR) more closely related to ion pump rhodopsins than other channelrhodopsins. ChRmine displays unique properties favorable for optogenetics including high light sensitivity, a broad, red-shifted activation spectrum, cation selectivity, and large photocurrents, while its slow closing kinetics impedes some applications. The structural basis for ChRmine function, or that of any other BCCR, is unknown. Here, we present cryo-EM structures of ChRmine in lipid nanodiscs in apo (opsin) and retinal-bound (rhodopsin) forms. The structures reveal an unprecedented trimeric architecture with a lipid filled central pore. Large electronegative cavities on either side of the membrane facilitate high conductance and selectivity for cations over protons. The retinal binding pocket structure suggests channel properties could be tuned with mutations and we identify ChRmine variants with ten-fold decreased and two-fold increased closing rates. A T119A mutant shows favorable properties relative to wild-type and previously reported ChRmine variants for optogenetics. These results provide insight into structural features that generate an ultra-potent microbial opsin and provide a platform for rational engineering of channelrhodopsins with improved properties that could expand the scale, depth, and precision of optogenetic experiments. | ||||||
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-構造の表示
ムービー |
ムービービューア |
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構造ビューア | 分子: MolmilJmol/JSmol |
-ダウンロードとリンク
-ダウンロード
PDBx/mmCIF形式 | 7sfk.cif.gz | 174.4 KB | 表示 | PDBx/mmCIF形式 |
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PDB形式 | pdb7sfk.ent.gz | 136.2 KB | 表示 | PDB形式 |
PDBx/mmJSON形式 | 7sfk.json.gz | ツリー表示 | PDBx/mmJSON形式 | |
その他 | その他のダウンロード |
-検証レポート
文書・要旨 | 7sfk_validation.pdf.gz | 1.3 MB | 表示 | wwPDB検証レポート |
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文書・詳細版 | 7sfk_full_validation.pdf.gz | 1.3 MB | 表示 | |
XML形式データ | 7sfk_validation.xml.gz | 33.7 KB | 表示 | |
CIF形式データ | 7sfk_validation.cif.gz | 43.4 KB | 表示 | |
アーカイブディレクトリ | https://data.pdbj.org/pub/pdb/validation_reports/sf/7sfk ftp://data.pdbj.org/pub/pdb/validation_reports/sf/7sfk | HTTPS FTP |
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