PDB-7r5o: 1.58 A STRUCTURE OF HUMAN APOFERRITIN OBTAINED FROM TITAN KRIOS 2...

基本情報

• 登録情報: データベース: PDB / ID: 7r5o
• タイトル: 1.58 A STRUCTURE OF HUMAN APOFERRITIN OBTAINED FROM TITAN KRIOS 2 AT eBIC, DLS UNDER COMMISSIONING SESSION CM26464-2
• 要素: Ferritin heavy chain, N-terminally processed
• キーワード: METAL BINDING PROTEIN / Protein standard

機能・相同性

分子機能:

iron ion sequestering activity / ferritin complex / Scavenging by Class A Receptors / Golgi Associated Vesicle Biogenesis / ferroxidase / autolysosome / negative regulation of ferroptosis / ferroxidase activity / negative regulation of fibroblast proliferation / ferric iron binding / autophagosome / iron ion transport / ferrous iron binding / Iron uptake and transport / tertiary granule lumen / ficolin-1-rich granule lumen / intracellular iron ion homeostasis / immune response / iron ion binding / negative regulation of cell population proliferation / Neutrophil degranulation / extracellular exosome / extracellular region / identical protein binding / nucleus / cytoplasm / cytosol

ドメイン・相同性:

Ferritin iron-binding regions signature 1. / Ferritin iron-binding regions signature 2. / Ferritin, conserved site / Ferritin / Ferritin-like diiron domain / Ferritin-like diiron domain profile. / Ferritin/DPS protein domain / Ferritin-like domain / Ferritin-like / Ferritin-like superfamily

構成要素:

Ferritin heavy chain

• 生物種: Homo sapiens (ヒト)
• 手法: 電子顕微鏡法 / 単粒子再構成法 / クライオ電子顕微鏡法 / 解像度: 1.58 Å
• データ登録者: Clare, D.K.

資金援助

英国, 1件

組織	認可番号	国
Not funded	cm26464-2	英国

• 引用: ジャーナル: Faraday Discuss / 年: 2022; タイトル: Application of super-resolution and correlative double sampling in cryo-electron microscopy.; 著者: Yuewen Sheng / Peter J Harrison / Vinod Vogirala / Zhengyi Yang / Claire Strain-Damerell / Thomas Frosio / Benjamin A Himes / C Alistair Siebert / Peijun Zhang / Daniel K Clare / ; 要旨: Developments in cryo-EM have allowed atomic or near-atomic resolution structure determination to become routine in single particle analysis (SPA). However, near-atomic resolution structures determined using cryo-electron tomography and sub-tomogram averaging (cryo-ET STA) are much less routine. In this paper, we show that collecting cryo-ET STA data using the same conditions as SPA, with both correlated double sampling (CDS) and the super-resolution mode, allowed apoferritin to be reconstructed out to the physical Nyquist frequency of the images. Even with just two tilt series, STA yields an apoferritin map at 2.9 Å resolution. These results highlight the exciting potential of cryo-ET STA in the future of protein structure determination. While processing SPA data recorded in super-resolution mode may yield structures surpassing the physical Nyquist limit, processing cryo-ET STA data in the super-resolution mode gave no additional resolution benefit. We further show that collecting SPA data in the super-resolution mode, with CDS activated, reduces the estimated -factor, leading to a reduction in the number of particles required to reach a target resolution without compromising the data size on disk and the area imaged in SerialEM. However, collecting SPA data in CDS does reduce throughput, given that a similar resolution structure, with a slightly larger -factor, is achievable with optimised parameters for speed in EPU (without CDS).

履歴

登録	2022年2月11日	登録サイト: PDBE / 処理サイト: PDBE
改定 1.0	2022年6月22日	Provider: repository / タイプ: Initial release
改定 1.1	2023年7月5日	Group: Database references / Refinement description カテゴリ: citation / citation_author / pdbx_initial_refinement_model Item: _citation.country / _citation.journal_abbrev / _citation.journal_id_ASTM / _citation.journal_id_CSD / _citation.journal_id_ISSN / _citation.journal_volume / _citation.page_first / _citation.page_last / _citation.pdbx_database_id_DOI / _citation.pdbx_database_id_PubMed / _citation.title / _citation.year

集合体

登録構造単位

A: Ferritin heavy chain, N-terminally processed

B: Ferritin heavy chain, N-terminally processed

C: Ferritin heavy chain, N-terminally processed

D: Ferritin heavy chain, N-terminally processed

E: Ferritin heavy chain, N-terminally processed

F: Ferritin heavy chain, N-terminally processed

G: Ferritin heavy chain, N-terminally processed

H: Ferritin heavy chain, N-terminally processed

I: Ferritin heavy chain, N-terminally processed

J: Ferritin heavy chain, N-terminally processed

K: Ferritin heavy chain, N-terminally processed

L: Ferritin heavy chain, N-terminally processed

M: Ferritin heavy chain, N-terminally processed

N: Ferritin heavy chain, N-terminally processed

O: Ferritin heavy chain, N-terminally processed

P: Ferritin heavy chain, N-terminally processed

Q: Ferritin heavy chain, N-terminally processed

R: Ferritin heavy chain, N-terminally processed

S: Ferritin heavy chain, N-terminally processed

T: Ferritin heavy chain, N-terminally processed

U: Ferritin heavy chain, N-terminally processed

V: Ferritin heavy chain, N-terminally processed

W: Ferritin heavy chain, N-terminally processed

X: Ferritin heavy chain, N-terminally processed

ヘテロ分子

概要:

• 486 kDa, 24 ポリマー

構成要素の詳細:

	分子量 (理論値)	分子数
合計 (水以外)	485,797	48
ポリマ－	485,246	24
非ポリマー	552	24
水	60,963	3384

1

概要:

• 登録構造と同一
• 登録者が定義した集合体
• 根拠: 電子顕微鏡法

対称操作:

タイプ	名称	対称操作	数
identity operation	1_555		1

非結晶学的対称性 (NCS)

NCSドメイン:

ID	Ens-ID	詳細
1	1	A
2	1	B
1	2	A
2	2	C
1	3	A
2	3	D
1	4	A
2	4	E
1	5	A
2	5	F
1	6	A
2	6	G
1	7	A
2	7	H
1	8	A
2	8	I
1	9	A
2	9	J
1	10	A
2	10	K
1	11	A
2	11	L
1	12	A
2	12	M
1	13	A
2	13	N
1	14	A
2	14	O
1	15	A
2	15	P
1	16	A
2	16	Q
1	17	A
2	17	R
1	18	A
2	18	S
1	19	A
2	19	T
1	20	A
2	20	U
1	21	A
2	21	V
1	22	A
2	22	W
1	23	A
2	23	X
1	24	B
2	24	C
1	25	B
2	25	D
1	26	B
2	26	E
1	27	B
2	27	F
1	28	B
2	28	G
1	29	B
2	29	H
1	30	B
2	30	I
1	31	B
2	31	J
1	32	B
2	32	K
1	33	B
2	33	L
1	34	B
2	34	M
1	35	B
2	35	N
1	36	B
2	36	O
1	37	B
2	37	P
1	38	B
2	38	Q
1	39	B
2	39	R
1	40	B
2	40	S
1	41	B
2	41	T
1	42	B
2	42	U
1	43	B
2	43	V
1	44	B
2	44	W
1	45	B
2	45	X
1	46	C
2	46	D
1	47	C
2	47	E
1	48	C
2	48	F
1	49	C
2	49	G
1	50	C
2	50	H
1	51	C
2	51	I
1	52	C
2	52	J
1	53	C
2	53	K
1	54	C
2	54	L
1	55	C
2	55	M
1	56	C
2	56	N
1	57	C
2	57	O
1	58	C
2	58	P
1	59	C
2	59	Q
1	60	C
2	60	R
1	61	C
2	61	S
1	62	C
2	62	T
1	63	C
2	63	U
1	64	C
2	64	V
1	65	C
2	65	W
1	66	C
2	66	X
1	67	D
2	67	E
1	68	D
2	68	F
1	69	D
2	69	G
1	70	D
2	70	H
1	71	D
2	71	I
1	72	D
2	72	J
1	73	D
2	73	K
1	74	D
2	74	L
1	75	D
2	75	M
1	76	D
2	76	N
1	77	D
2	77	O
1	78	D
2	78	P
1	79	D
2	79	Q
1	80	D
2	80	R
1	81	D
2	81	S
1	82	D
2	82	T
1	83	D
2	83	U
1	84	D
2	84	V
1	85	D
2	85	W
1	86	D
2	86	X
1	87	E
2	87	F
1	88	E
2	88	G
1	89	E
2	89	H
1	90	E
2	90	I
1	91	E
2	91	J
1	92	E
2	92	K
1	93	E
2	93	L
1	94	E
2	94	M
1	95	E
2	95	N
1	96	E
2	96	O
1	97	E
2	97	P
1	98	E
2	98	Q
1	99	E
2	99	R
1	100	E
2	100	S
1	101	E
2	101	T
1	102	E
2	102	U
1	103	E
2	103	V
1	104	E
2	104	W
1	105	E
2	105	X
1	106	F
2	106	G
1	107	F
2	107	H
1	108	F
2	108	I
1	109	F
2	109	J
1	110	F
2	110	K
1	111	F
2	111	L
1	112	F
2	112	M
1	113	F
2	113	N
1	114	F
2	114	O
1	115	F
2	115	P
1	116	F
2	116	Q
1	117	F
2	117	R
1	118	F
2	118	S
1	119	F
2	119	T
1	120	F
2	120	U
1	121	F
2	121	V
1	122	F
2	122	W
1	123	F
2	123	X
1	124	G
2	124	H
1	125	G
2	125	I
1	126	G
2	126	J
1	127	G
2	127	K
1	128	G
2	128	L
1	129	G
2	129	M
1	130	G
2	130	N
1	131	G
2	131	O
1	132	G
2	132	P
1	133	G
2	133	Q
1	134	G
2	134	R
1	135	G
2	135	S
1	136	G
2	136	T
1	137	G
2	137	U
1	138	G
2	138	V
1	139	G
2	139	W
1	140	G
2	140	X
1	141	H
2	141	I
1	142	H
2	142	J
1	143	H
2	143	K
1	144	H
2	144	L
1	145	H
2	145	M
1	146	H
2	146	N
1	147	H
2	147	O
1	148	H
2	148	P
1	149	H
2	149	Q
1	150	H
2	150	R
1	151	H
2	151	S
1	152	H
2	152	T
1	153	H
2	153	U
1	154	H
2	154	V
1	155	H
2	155	W
1	156	H
2	156	X
1	157	I
2	157	J
1	158	I
2	158	K
1	159	I
2	159	L
1	160	I
2	160	M
1	161	I
2	161	N
1	162	I
2	162	O
1	163	I
2	163	P
1	164	I
2	164	Q
1	165	I
2	165	R
1	166	I
2	166	S
1	167	I
2	167	T
1	168	I
2	168	U
1	169	I
2	169	V
1	170	I
2	170	W
1	171	I
2	171	X
1	172	J
2	172	K
1	173	J
2	173	L
1	174	J
2	174	M
1	175	J
2	175	N
1	176	J
2	176	O
1	177	J
2	177	P
1	178	J
2	178	Q
1	179	J
2	179	R
1	180	J
2	180	S
1	181	J
2	181	T
1	182	J
2	182	U
1	183	J
2	183	V
1	184	J
2	184	W
1	185	J
2	185	X
1	186	K
2	186	L
1	187	K
2	187	M
1	188	K
2	188	N
1	189	K
2	189	O
1	190	K
2	190	P
1	191	K
2	191	Q
1	192	K
2	192	R
1	193	K
2	193	S
1	194	K
2	194	T
1	195	K
2	195	U
1	196	K
2	196	V
1	197	K
2	197	W
1	198	K
2	198	X
1	199	L
2	199	M
1	200	L
2	200	N
1	201	L
2	201	O
1	202	L
2	202	P
1	203	L
2	203	Q
1	204	L
2	204	R
1	205	L
2	205	S
1	206	L
2	206	T
1	207	L
2	207	U
1	208	L
2	208	V
1	209	L
2	209	W
1	210	L
2	210	X
1	211	M
2	211	N
1	212	M
2	212	O
1	213	M
2	213	P
1	214	M
2	214	Q
1	215	M
2	215	R
1	216	M
2	216	S
1	217	M
2	217	T
1	218	M
2	218	U
1	219	M
2	219	V
1	220	M
2	220	W
1	221	M
2	221	X
1	222	N
2	222	O
1	223	N
2	223	P
1	224	N
2	224	Q
1	225	N
2	225	R
1	226	N
2	226	S
1	227	N
2	227	T
1	228	N
2	228	U
1	229	N
2	229	V
1	230	N
2	230	W
1	231	N
2	231	X
1	232	O
2	232	P
1	233	O
2	233	Q
1	234	O
2	234	R
1	235	O
2	235	S
1	236	O
2	236	T
1	237	O
2	237	U
1	238	O
2	238	V
1	239	O
2	239	W
1	240	O
2	240	X
1	241	P
2	241	Q
1	242	P
2	242	R
1	243	P
2	243	S
1	244	P
2	244	T
1	245	P
2	245	U
1	246	P
2	246	V
1	247	P
2	247	W
1	248	P
2	248	X
1	249	Q
2	249	R
1	250	Q
2	250	S
1	251	Q
2	251	T
1	252	Q
2	252	U
1	253	Q
2	253	V
1	254	Q
2	254	W
1	255	Q
2	255	X
1	256	R
2	256	S
1	257	R
2	257	T
1	258	R
2	258	U
1	259	R
2	259	V
1	260	R
2	260	W
1	261	R
2	261	X
1	262	S
2	262	T
1	263	S
2	263	U
1	264	S
2	264	V
1	265	S
2	265	W
1	266	S
2	266	X
1	267	T
2	267	U
1	268	T
2	268	V
1	269	T
2	269	W
1	270	T
2	270	X
1	271	U
2	271	V
1	272	U
2	272	W
1	273	U
2	273	X
1	274	V
2	274	W
1	275	V
2	275	X
1	276	W
2	276	X

NCSドメイン領域:

Dom-ID	Component-ID	Ens-ID	Refine code	Auth asym-ID	Auth seq-ID
1	0	1	0	A	4 - 176
2	0	1	0	B	4 - 176
1	0	2	0	A	4 - 176
2	0	2	0	C	4 - 176
1	0	3	0	A	4 - 176
2	0	3	0	D	4 - 176
1	0	4	0	A	4 - 176
2	0	4	0	E	4 - 176
1	0	5	0	A	4 - 176
2	0	5	0	F	4 - 176
1	0	6	0	A	4 - 176
2	0	6	0	G	4 - 176
1	0	7	0	A	4 - 176
2	0	7	0	H	4 - 176
1	0	8	0	A	4 - 176
2	0	8	0	I	4 - 176
1	0	9	0	A	4 - 176
2	0	9	0	J	4 - 176
1	0	10	0	A	4 - 176
2	0	10	0	K	4 - 176
1	0	11	0	A	4 - 176
2	0	11	0	L	4 - 176
1	0	12	0	A	4 - 176
2	0	12	0	M	4 - 176
1	0	13	0	A	4 - 176
2	0	13	0	N	4 - 176
1	0	14	0	A	4 - 176
2	0	14	0	O	4 - 176
1	0	15	0	A	4 - 176
2	0	15	0	P	4 - 176
1	0	16	0	A	4 - 176
2	0	16	0	Q	4 - 176
1	0	17	0	A	4 - 176
2	0	17	0	R	4 - 176
1	0	18	0	A	4 - 176
2	0	18	0	S	4 - 176
1	0	19	0	A	4 - 176
2	0	19	0	T	4 - 176
1	0	20	0	A	4 - 176
2	0	20	0	U	4 - 176
1	0	21	0	A	4 - 176
2	0	21	0	V	4 - 176
1	0	22	0	A	4 - 176
2	0	22	0	W	4 - 176
1	0	23	0	A	4 - 176
2	0	23	0	X	4 - 176
1	0	24	0	B	4 - 176
2	0	24	0	C	4 - 176
1	0	25	0	B	4 - 176
2	0	25	0	D	4 - 176
1	0	26	0	B	4 - 176
2	0	26	0	E	4 - 176
1	0	27	0	B	4 - 176
2	0	27	0	F	4 - 176
1	0	28	0	B	4 - 176
2	0	28	0	G	4 - 176
1	0	29	0	B	4 - 176
2	0	29	0	H	4 - 176
1	0	30	0	B	4 - 176
2	0	30	0	I	4 - 176
1	0	31	0	B	4 - 176
2	0	31	0	J	4 - 176
1	0	32	0	B	4 - 176
2	0	32	0	K	4 - 176
1	0	33	0	B	4 - 176
2	0	33	0	L	4 - 176
1	0	34	0	B	4 - 176
2	0	34	0	M	4 - 176
1	0	35	0	B	4 - 176
2	0	35	0	N	4 - 176
1	0	36	0	B	4 - 176
2	0	36	0	O	4 - 176
1	0	37	0	B	4 - 176
2	0	37	0	P	4 - 176
1	0	38	0	B	4 - 176
2	0	38	0	Q	4 - 176
1	0	39	0	B	4 - 176
2	0	39	0	R	4 - 176
1	0	40	0	B	4 - 176
2	0	40	0	S	4 - 176
1	0	41	0	B	4 - 176
2	0	41	0	T	4 - 176
1	0	42	0	B	4 - 176
2	0	42	0	U	4 - 176
1	0	43	0	B	4 - 176
2	0	43	0	V	4 - 176
1	0	44	0	B	4 - 176
2	0	44	0	W	4 - 176
1	0	45	0	B	4 - 176
2	0	45	0	X	4 - 176
1	0	46	0	C	4 - 176
2	0	46	0	D	4 - 176
1	0	47	0	C	4 - 176
2	0	47	0	E	4 - 176
1	0	48	0	C	4 - 176
2	0	48	0	F	4 - 176
1	0	49	0	C	4 - 176
2	0	49	0	G	4 - 176
1	0	50	0	C	4 - 176
2	0	50	0	H	4 - 176
1	0	51	0	C	4 - 176
2	0	51	0	I	4 - 176
1	0	52	0	C	4 - 176
2	0	52	0	J	4 - 176
1	0	53	0	C	4 - 176
2	0	53	0	K	4 - 176
1	0	54	0	C	4 - 176
2	0	54	0	L	4 - 176
1	0	55	0	C	4 - 176
2	0	55	0	M	4 - 176
1	0	56	0	C	4 - 176
2	0	56	0	N	4 - 176
1	0	57	0	C	4 - 176
2	0	57	0	O	4 - 176
1	0	58	0	C	4 - 176
2	0	58	0	P	4 - 176
1	0	59	0	C	4 - 176
2	0	59	0	Q	4 - 176
1	0	60	0	C	4 - 176
2	0	60	0	R	4 - 176
1	0	61	0	C	4 - 176
2	0	61	0	S	4 - 176
1	0	62	0	C	4 - 176
2	0	62	0	T	4 - 176
1	0	63	0	C	4 - 176
2	0	63	0	U	4 - 176
1	0	64	0	C	4 - 176
2	0	64	0	V	4 - 176
1	0	65	0	C	4 - 176
2	0	65	0	W	4 - 176
1	0	66	0	C	4 - 176
2	0	66	0	X	4 - 176
1	0	67	0	D	4 - 176
2	0	67	0	E	4 - 176
1	0	68	0	D	4 - 176
2	0	68	0	F	4 - 176
1	0	69	0	D	4 - 176
2	0	69	0	G	4 - 176
1	0	70	0	D	4 - 176
2	0	70	0	H	4 - 176
1	0	71	0	D	4 - 176
2	0	71	0	I	4 - 176
1	0	72	0	D	4 - 176
2	0	72	0	J	4 - 176
1	0	73	0	D	4 - 176
2	0	73	0	K	4 - 176
1	0	74	0	D	4 - 176
2	0	74	0	L	4 - 176
1	0	75	0	D	4 - 176
2	0	75	0	M	4 - 176
1	0	76	0	D	4 - 176
2	0	76	0	N	4 - 176
1	0	77	0	D	4 - 176
2	0	77	0	O	4 - 176
1	0	78	0	D	4 - 176
2	0	78	0	P	4 - 176
1	0	79	0	D	4 - 176
2	0	79	0	Q	4 - 176
1	0	80	0	D	4 - 176
2	0	80	0	R	4 - 176
1	0	81	0	D	4 - 176
2	0	81	0	S	4 - 176
1	0	82	0	D	4 - 176
2	0	82	0	T	4 - 176
1	0	83	0	D	4 - 176
2	0	83	0	U	4 - 176
1	0	84	0	D	4 - 176
2	0	84	0	V	4 - 176
1	0	85	0	D	4 - 176
2	0	85	0	W	4 - 176
1	0	86	0	D	4 - 176
2	0	86	0	X	4 - 176
1	0	87	0	E	4 - 176
2	0	87	0	F	4 - 176
1	0	88	0	E	4 - 176
2	0	88	0	G	4 - 176
1	0	89	0	E	4 - 176
2	0	89	0	H	4 - 176
1	0	90	0	E	4 - 176
2	0	90	0	I	4 - 176
1	0	91	0	E	4 - 176
2	0	91	0	J	4 - 176
1	0	92	0	E	4 - 176
2	0	92	0	K	4 - 176
1	0	93	0	E	4 - 176
2	0	93	0	L	4 - 176
1	0	94	0	E	4 - 176
2	0	94	0	M	4 - 176
1	0	95	0	E	4 - 176
2	0	95	0	N	4 - 176
1	0	96	0	E	4 - 176
2	0	96	0	O	4 - 176
1	0	97	0	E	4 - 176
2	0	97	0	P	4 - 176
1	0	98	0	E	4 - 176
2	0	98	0	Q	4 - 176
1	0	99	0	E	4 - 176
2	0	99	0	R	4 - 176
1	0	100	0	E	4 - 176
2	0	100	0	S	4 - 176
1	0	101	0	E	4 - 176
2	0	101	0	T	4 - 176
1	0	102	0	E	4 - 176
2	0	102	0	U	4 - 176
1	0	103	0	E	4 - 176
2	0	103	0	V	4 - 176
1	0	104	0	E	4 - 176
2	0	104	0	W	4 - 176
1	0	105	0	E	4 - 176
2	0	105	0	X	4 - 176
1	0	106	0	F	4 - 176
2	0	106	0	G	4 - 176
1	0	107	0	F	4 - 176
2	0	107	0	H	4 - 176
1	0	108	0	F	4 - 176
2	0	108	0	I	4 - 176
1	0	109	0	F	4 - 176
2	0	109	0	J	4 - 176
1	0	110	0	F	4 - 176
2	0	110	0	K	4 - 176
1	0	111	0	F	4 - 176
2	0	111	0	L	4 - 176
1	0	112	0	F	4 - 176
2	0	112	0	M	4 - 176
1	0	113	0	F	4 - 176
2	0	113	0	N	4 - 176
1	0	114	0	F	4 - 176
2	0	114	0	O	4 - 176
1	0	115	0	F	4 - 176
2	0	115	0	P	4 - 176
1	0	116	0	F	4 - 176
2	0	116	0	Q	4 - 176
1	0	117	0	F	4 - 176
2	0	117	0	R	4 - 176
1	0	118	0	F	4 - 176
2	0	118	0	S	4 - 176
1	0	119	0	F	4 - 176
2	0	119	0	T	4 - 176
1	0	120	0	F	4 - 176
2	0	120	0	U	4 - 176
1	0	121	0	F	4 - 176
2	0	121	0	V	4 - 176
1	0	122	0	F	4 - 176
2	0	122	0	W	4 - 176
1	0	123	0	F	4 - 176
2	0	123	0	X	4 - 176
1	0	124	0	G	4 - 176
2	0	124	0	H	4 - 176
1	0	125	0	G	4 - 176
2	0	125	0	I	4 - 176
1	0	126	0	G	4 - 176
2	0	126	0	J	4 - 176
1	0	127	0	G	4 - 176
2	0	127	0	K	4 - 176
1	0	128	0	G	4 - 176
2	0	128	0	L	4 - 176
1	0	129	0	G	4 - 176
2	0	129	0	M	4 - 176
1	0	130	0	G	4 - 176
2	0	130	0	N	4 - 176
1	0	131	0	G	4 - 176
2	0	131	0	O	4 - 176
1	0	132	0	G	4 - 176
2	0	132	0	P	4 - 176
1	0	133	0	G	4 - 176
2	0	133	0	Q	4 - 176
1	0	134	0	G	4 - 176
2	0	134	0	R	4 - 176
1	0	135	0	G	4 - 176
2	0	135	0	S	4 - 176
1	0	136	0	G	4 - 176
2	0	136	0	T	4 - 176
1	0	137	0	G	4 - 176
2	0	137	0	U	4 - 176
1	0	138	0	G	4 - 176
2	0	138	0	V	4 - 176
1	0	139	0	G	4 - 176
2	0	139	0	W	4 - 176
1	0	140	0	G	4 - 176
2	0	140	0	X	4 - 176
1	0	141	0	H	4 - 176
2	0	141	0	I	4 - 176
1	0	142	0	H	4 - 176
2	0	142	0	J	4 - 176
1	0	143	0	H	4 - 176
2	0	143	0	K	4 - 176
1	0	144	0	H	4 - 176
2	0	144	0	L	4 - 176
1	0	145	0	H	4 - 176
2	0	145	0	M	4 - 176
1	0	146	0	H	4 - 176
2	0	146	0	N	4 - 176
1	0	147	0	H	4 - 176
2	0	147	0	O	4 - 176
1	0	148	0	H	4 - 176
2	0	148	0	P	4 - 176
1	0	149	0	H	4 - 176
2	0	149	0	Q	4 - 176
1	0	150	0	H	4 - 176
2	0	150	0	R	4 - 176
1	0	151	0	H	4 - 176
2	0	151	0	S	4 - 176
1	0	152	0	H	4 - 176
2	0	152	0	T	4 - 176
1	0	153	0	H	4 - 176
2	0	153	0	U	4 - 176
1	0	154	0	H	4 - 176
2	0	154	0	V	4 - 176
1	0	155	0	H	4 - 176
2	0	155	0	W	4 - 176
1	0	156	0	H	4 - 176
2	0	156	0	X	4 - 176
1	0	157	0	I	4 - 176
2	0	157	0	J	4 - 176
1	0	158	0	I	4 - 176
2	0	158	0	K	4 - 176
1	0	159	0	I	4 - 176
2	0	159	0	L	4 - 176
1	0	160	0	I	4 - 176
2	0	160	0	M	4 - 176
1	0	161	0	I	4 - 176
2	0	161	0	N	4 - 176
1	0	162	0	I	4 - 176
2	0	162	0	O	4 - 176
1	0	163	0	I	4 - 176
2	0	163	0	P	4 - 176
1	0	164	0	I	4 - 176
2	0	164	0	Q	4 - 176
1	0	165	0	I	4 - 176
2	0	165	0	R	4 - 176
1	0	166	0	I	4 - 176
2	0	166	0	S	4 - 176
1	0	167	0	I	4 - 176
2	0	167	0	T	4 - 176
1	0	168	0	I	4 - 176
2	0	168	0	U	4 - 176
1	0	169	0	I	4 - 176
2	0	169	0	V	4 - 176
1	0	170	0	I	4 - 176
2	0	170	0	W	4 - 176
1	0	171	0	I	4 - 176
2	0	171	0	X	4 - 176
1	0	172	0	J	4 - 176
2	0	172	0	K	4 - 176
1	0	173	0	J	4 - 176
2	0	173	0	L	4 - 176
1	0	174	0	J	4 - 176
2	0	174	0	M	4 - 176
1	0	175	0	J	4 - 176
2	0	175	0	N	4 - 176
1	0	176	0	J	4 - 176
2	0	176	0	O	4 - 176
1	0	177	0	J	4 - 176
2	0	177	0	P	4 - 176
1	0	178	0	J	4 - 176
2	0	178	0	Q	4 - 176
1	0	179	0	J	4 - 176
2	0	179	0	R	4 - 176
1	0	180	0	J	4 - 176
2	0	180	0	S	4 - 176
1	0	181	0	J	4 - 176
2	0	181	0	T	4 - 176
1	0	182	0	J	4 - 176
2	0	182	0	U	4 - 176
1	0	183	0	J	4 - 176
2	0	183	0	V	4 - 176
1	0	184	0	J	4 - 176
2	0	184	0	W	4 - 176
1	0	185	0	J	4 - 176
2	0	185	0	X	4 - 176
1	0	186	0	K	4 - 176
2	0	186	0	L	4 - 176
1	0	187	0	K	4 - 176
2	0	187	0	M	4 - 176
1	0	188	0	K	4 - 176
2	0	188	0	N	4 - 176
1	0	189	0	K	4 - 176
2	0	189	0	O	4 - 176
1	0	190	0	K	4 - 176
2	0	190	0	P	4 - 176
1	0	191	0	K	4 - 176
2	0	191	0	Q	4 - 176
1	0	192	0	K	4 - 176
2	0	192	0	R	4 - 176
1	0	193	0	K	4 - 176
2	0	193	0	S	4 - 176
1	0	194	0	K	4 - 176
2	0	194	0	T	4 - 176
1	0	195	0	K	4 - 176
2	0	195	0	U	4 - 176
1	0	196	0	K	4 - 176
2	0	196	0	V	4 - 176
1	0	197	0	K	4 - 176
2	0	197	0	W	4 - 176
1	0	198	0	K	4 - 176
2	0	198	0	X	4 - 176
1	0	199	0	L	4 - 176
2	0	199	0	M	4 - 176
1	0	200	0	L	4 - 176
2	0	200	0	N	4 - 176
1	0	201	0	L	4 - 176
2	0	201	0	O	4 - 176
1	0	202	0	L	4 - 176
2	0	202	0	P	4 - 176
1	0	203	0	L	4 - 176
2	0	203	0	Q	4 - 176
1	0	204	0	L	4 - 176
2	0	204	0	R	4 - 176
1	0	205	0	L	4 - 176
2	0	205	0	S	4 - 176
1	0	206	0	L	4 - 176
2	0	206	0	T	4 - 176
1	0	207	0	L	4 - 176
2	0	207	0	U	4 - 176
1	0	208	0	L	4 - 176
2	0	208	0	V	4 - 176
1	0	209	0	L	4 - 176
2	0	209	0	W	4 - 176
1	0	210	0	L	4 - 176
2	0	210	0	X	4 - 176
1	0	211	0	M	4 - 176
2	0	211	0	N	4 - 176
1	0	212	0	M	4 - 176
2	0	212	0	O	4 - 176
1	0	213	0	M	4 - 176
2	0	213	0	P	4 - 176
1	0	214	0	M	4 - 176
2	0	214	0	Q	4 - 176
1	0	215	0	M	4 - 176
2	0	215	0	R	4 - 176
1	0	216	0	M	4 - 176
2	0	216	0	S	4 - 176
1	0	217	0	M	4 - 176
2	0	217	0	T	4 - 176
1	0	218	0	M	4 - 176
2	0	218	0	U	4 - 176
1	0	219	0	M	4 - 176
2	0	219	0	V	4 - 176
1	0	220	0	M	4 - 176
2	0	220	0	W	4 - 176
1	0	221	0	M	4 - 176
2	0	221	0	X	4 - 176
1	0	222	0	N	4 - 176
2	0	222	0	O	4 - 176
1	0	223	0	N	4 - 176
2	0	223	0	P	4 - 176
1	0	224	0	N	4 - 176
2	0	224	0	Q	4 - 176
1	0	225	0	N	4 - 176
2	0	225	0	R	4 - 176
1	0	226	0	N	4 - 176
2	0	226	0	S	4 - 176
1	0	227	0	N	4 - 176
2	0	227	0	T	4 - 176
1	0	228	0	N	4 - 176
2	0	228	0	U	4 - 176
1	0	229	0	N	4 - 176
2	0	229	0	V	4 - 176
1	0	230	0	N	4 - 176
2	0	230	0	W	4 - 176
1	0	231	0	N	4 - 176
2	0	231	0	X	4 - 176
1	0	232	0	O	4 - 176
2	0	232	0	P	4 - 176
1	0	233	0	O	4 - 176
2	0	233	0	Q	4 - 176
1	0	234	0	O	4 - 176
2	0	234	0	R	4 - 176
1	0	235	0	O	4 - 176
2	0	235	0	S	4 - 176
1	0	236	0	O	4 - 176
2	0	236	0	T	4 - 176
1	0	237	0	O	4 - 176
2	0	237	0	U	4 - 176
1	0	238	0	O	4 - 176
2	0	238	0	V	4 - 176
1	0	239	0	O	4 - 176
2	0	239	0	W	4 - 176
1	0	240	0	O	4 - 176
2	0	240	0	X	4 - 176
1	0	241	0	P	4 - 176
2	0	241	0	Q	4 - 176
1	0	242	0	P	4 - 176
2	0	242	0	R	4 - 176
1	0	243	0	P	4 - 176
2	0	243	0	S	4 - 176
1	0	244	0	P	4 - 176
2	0	244	0	T	4 - 176
1	0	245	0	P	4 - 176
2	0	245	0	U	4 - 176
1	0	246	0	P	4 - 176
2	0	246	0	V	4 - 176
1	0	247	0	P	4 - 176
2	0	247	0	W	4 - 176
1	0	248	0	P	4 - 176
2	0	248	0	X	4 - 176
1	0	249	0	Q	4 - 176
2	0	249	0	R	4 - 176
1	0	250	0	Q	4 - 176
2	0	250	0	S	4 - 176
1	0	251	0	Q	4 - 176
2	0	251	0	T	4 - 176
1	0	252	0	Q	4 - 176
2	0	252	0	U	4 - 176
1	0	253	0	Q	4 - 176
2	0	253	0	V	4 - 176
1	0	254	0	Q	4 - 176
2	0	254	0	W	4 - 176
1	0	255	0	Q	4 - 176
2	0	255	0	X	4 - 176
1	0	256	0	R	4 - 176
2	0	256	0	S	4 - 176
1	0	257	0	R	4 - 176
2	0	257	0	T	4 - 176
1	0	258	0	R	4 - 176
2	0	258	0	U	4 - 176
1	0	259	0	R	4 - 176
2	0	259	0	V	4 - 176
1	0	260	0	R	4 - 176
2	0	260	0	W	4 - 176
1	0	261	0	R	4 - 176
2	0	261	0	X	4 - 176
1	0	262	0	S	4 - 176
2	0	262	0	T	4 - 176
1	0	263	0	S	4 - 176
2	0	263	0	U	4 - 176
1	0	264	0	S	4 - 176
2	0	264	0	V	4 - 176
1	0	265	0	S	4 - 176
2	0	265	0	W	4 - 176
1	0	266	0	S	4 - 176
2	0	266	0	X	4 - 176
1	0	267	0	T	4 - 176
2	0	267	0	U	4 - 176
1	0	268	0	T	4 - 176
2	0	268	0	V	4 - 176
1	0	269	0	T	4 - 176
2	0	269	0	W	4 - 176
1	0	270	0	T	4 - 176
2	0	270	0	X	4 - 176
1	0	271	0	U	4 - 176
2	0	271	0	V	4 - 176
1	0	272	0	U	4 - 176
2	0	272	0	W	4 - 176
1	0	273	0	U	4 - 176
2	0	273	0	X	4 - 176
1	0	274	0	V	4 - 176
2	0	274	0	W	4 - 176
1	0	275	0	V	4 - 176
2	0	275	0	X	4 - 176
1	0	276	0	W	4 - 176
2	0	276	0	X	4 - 176

NCSアンサンブル:

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要素

#1: タンパク質

A B C D E F G H I J K L M N O P Q R S ...
Ferritin heavy chain, N-terminally processed

詳細:

分子量: 20218.570 Da / 分子数: 24 / 由来タイプ: 組換発現 / 詳細: Bacterial expression / 由来: (組換発現) Homo sapiens (ヒト) / 遺伝子: FTH1, FTH, FTHL6, OK/SW-cl.84, PIG15 / 発現宿主: Escherichia coli (大腸菌) / 参照: UniProt: P02794

#2: 化合物

A-500 B-500 C-500 D-500 E-500 F-500 G-500 H-500 I-500 J-500 K-500 L-500 M-500 N-500 O-500 P-500 Q-500 R-500 S-500 T-500 ...
ChemComp-NA / SODIUM ION

詳細:

分子量: 22.990 Da / 分子数: 24 / 由来タイプ: 合成 / 式: Na

#3: 水

ChemComp-HOH / water

詳細:

分子量: 18.015 Da / 分子数: 3384 / 由来タイプ: 天然 / 式: H₂O

• 研究の焦点であるリガンドがあるか: N

実験情報

実験

• 実験: 手法: 電子顕微鏡法
• EM実験: 試料の集合状態: PARTICLE / ３次元再構成法: 単粒子再構成法

試料調製

• 構成要素: 名称: human Apoferritin / タイプ: COMPLEX / 詳細: Heavy chain / Entity ID: #1 / 由来: RECOMBINANT
• 分子量: 値: 0.5 MDa / 実験値: NO
• 由来(天然): 生物種: Homo sapiens (ヒト)
• 由来(組換発現): 生物種: Escherichia coli (大腸菌) / 株: D3A
• 緩衝液: pH: 7.5 / 詳細: 50 mM Tris-HCl, pH 7.5, 100 mM NaCl, 1 mM TCEP
• 試料: 濃度: 0.01 mg/ml / 包埋: NO / シャドウイング: NO / 染色: NO / 凍結: YES; 詳細: The apoferritin sample was applied to in-house graphene-coated Quantifoil r2/2 EM grid
• 試料支持: グリッドの材料: COPPER / グリッドのサイズ: 300 divisions/in. / グリッドのタイプ: Quantifoil R2/2
• 急速凍結: 装置: FEI VITROBOT MARK IV / 凍結剤: ETHANE / 湿度: 100 % / 凍結前の試料温度: 277 K / 詳細: blot time of 2.5 seconds

電子顕微鏡撮影

• 実験機器: モデル: Titan Krios / 画像提供: FEI Company
• 顕微鏡: モデル: FEI TITAN KRIOS
• 電子銃: 電子線源: FIELD EMISSION GUN / 加速電圧: 300 kV / 照射モード: FLOOD BEAM
• 電子レンズ: モード: BRIGHT FIELD / 倍率（公称値）: 130000 X / 最大 デフォーカス（公称値）: 2000 nm / 最小 デフォーカス（公称値）: 500 nm / Cs: 2.7 mm / C2レンズ絞り径: 50 µm / アライメント法: COMA FREE
• 試料ホルダ: 凍結剤: NITROGEN; 試料ホルダーモデル: FEI TITAN KRIOS AUTOGRID HOLDER
• 撮影: 平均露光時間: 2.5 sec. / 電子線照射量: 34 e/Ų; フィルム・検出器のモデル: GATAN K3 BIOQUANTUM (6k x 4k); 撮影したグリッド数: 1 / 実像数: 2295
• 電子光学装置: エネルギーフィルター名称: GIF Quantum LS / エネルギーフィルタースリット幅: 20 eV
• 画像スキャン: サンプリングサイズ: 5 µm / 横: 5760 / 縦: 4092

解析

• ソフトウェア: 名称: REFMAC / バージョン: 5.8.0267 / 分類: 精密化

EMソフトウェア

ID	名称	バージョン	カテゴリ
4	CTFFIND		CTF補正
7	UCSF Chimera		モデルフィッティング
9	RELION	3.1	初期オイラー角割当
10	RELION	3.1	最終オイラー角割当
11	RELION	3.1	分類
12	RELION	3.1	３次元再構成
13	REFMAC		モデル精密化

• CTF補正: 詳細: Done in Relion / タイプ: PHASE FLIPPING AND AMPLITUDE CORRECTION
• 対称性: 点対称性: O (正8面体型対称)
• 3次元再構成: 解像度: 1.58 Å / 解像度の算出法: FSC 0.143 CUT-OFF / 粒子像の数: 250000 / アルゴリズム: BACK PROJECTION / クラス平均像の数: 1 / 対称性のタイプ: POINT
• 原子モデル構築: プロトコル: FLEXIBLE FIT / 空間: RECIPROCAL / Target criteria: Correlation coefficient

原子モデル構築

PDB-ID: 6Z6U

6z6u

精密化

解像度: 1.6→132.51 Å / Cor.coef. F_o:F_c: 0.853 / SU B: 2.047 / SU ML: 0.061 / ESU R: 0.075
立体化学のターゲット値: MAXIMUM LIKELIHOOD WITH PHASES

	Rfactor	反射数	%反射
Rwork	0.24461	-	-
obs	0.24461	1189862	100 %

• 溶媒の処理: 溶媒モデル: PARAMETERS FOR MASK CACLULATION

原子変位パラメータ

B_iso mean: 22.382 Ų

	Baniso -1	Baniso -2	Baniso -3
1-	0 Å2	0 Å2	-0 Å2
2-	-	0 Å2	0 Å2
3-	-	-	-0 Å2

• 精密化ステップ: サイクル: 1 / 合計: 38520

拘束条件

Refine-ID	タイプ	Dev ideal	Dev ideal target	数
ELECTRON MICROSCOPY	r_bond_refined_d	0.02	0.012	35976
ELECTRON MICROSCOPY	r_bond_other_d
ELECTRON MICROSCOPY	r_angle_refined_deg	2.481	1.633	48648
ELECTRON MICROSCOPY	r_angle_other_deg
ELECTRON MICROSCOPY	r_dihedral_angle_1_deg	4.689	5	4464
ELECTRON MICROSCOPY	r_dihedral_angle_2_deg	37.186	23.83	2256
ELECTRON MICROSCOPY	r_dihedral_angle_3_deg	11.147	15	6744
ELECTRON MICROSCOPY	r_dihedral_angle_4_deg	20.567	15	192
ELECTRON MICROSCOPY	r_chiral_restr	0.193	0.2	4416
ELECTRON MICROSCOPY	r_gen_planes_refined	0.014	0.02	27936
ELECTRON MICROSCOPY	r_gen_planes_other
ELECTRON MICROSCOPY	r_nbd_refined
ELECTRON MICROSCOPY	r_nbd_other
ELECTRON MICROSCOPY	r_nbtor_refined
ELECTRON MICROSCOPY	r_nbtor_other
ELECTRON MICROSCOPY	r_xyhbond_nbd_refined
ELECTRON MICROSCOPY	r_xyhbond_nbd_other
ELECTRON MICROSCOPY	r_metal_ion_refined
ELECTRON MICROSCOPY	r_metal_ion_other
ELECTRON MICROSCOPY	r_symmetry_vdw_refined
ELECTRON MICROSCOPY	r_symmetry_vdw_other
ELECTRON MICROSCOPY	r_symmetry_hbond_refined
ELECTRON MICROSCOPY	r_symmetry_hbond_other
ELECTRON MICROSCOPY	r_symmetry_metal_ion_refined
ELECTRON MICROSCOPY	r_symmetry_metal_ion_other
ELECTRON MICROSCOPY	r_mcbond_it	2.696	1.559	16992
ELECTRON MICROSCOPY	r_mcbond_other
ELECTRON MICROSCOPY	r_mcangle_it	4.306	2.331	21312
ELECTRON MICROSCOPY	r_mcangle_other
ELECTRON MICROSCOPY	r_scbond_it	6.979	2.425	18984
ELECTRON MICROSCOPY	r_scbond_other
ELECTRON MICROSCOPY	r_scangle_it
ELECTRON MICROSCOPY	r_scangle_other
ELECTRON MICROSCOPY	r_long_range_B_refined	13.209	36.108	185873
ELECTRON MICROSCOPY	r_long_range_B_other
ELECTRON MICROSCOPY	r_rigid_bond_restr
ELECTRON MICROSCOPY	r_sphericity_free
ELECTRON MICROSCOPY	r_sphericity_bonded

Refine LS restraints NCS

Refine-ID: ELECTRON MICROSCOPY / タイプ: interatomic distance / Rms dev position: 0.03 Å / Weight position: 0.05

Ens-ID	Dom-ID	Auth asym-ID	数
1	1	A	12674
1	2	B	12674
2	1	A	12674
2	2	C	12674
3	1	A	12674
3	2	D	12674
4	1	A	12674
4	2	E	12674
5	1	A	12670
5	2	F	12670
6	1	A	12672
6	2	G	12672
7	1	A	12672
7	2	H	12672
8	1	A	12644
8	2	I	12644
9	1	A	12644
9	2	J	12644
10	1	A	12644
10	2	K	12644
11	1	A	12644
11	2	L	12644
12	1	A	12644
12	2	M	12644
13	1	A	12644
13	2	N	12644
14	1	A	12644
14	2	O	12644
15	1	A	12644
15	2	P	12644
16	1	A	12672
16	2	Q	12672
17	1	A	12672
17	2	R	12672
18	1	A	12672
18	2	S	12672
19	1	A	12672
19	2	T	12672
20	1	A	12644
20	2	U	12644
21	1	A	12644
21	2	V	12644
22	1	A	12644
22	2	W	12644
23	1	A	12644
23	2	X	12644
24	1	B	12674
24	2	C	12674
25	1	B	12674
25	2	D	12674
26	1	B	12674
26	2	E	12674
27	1	B	12670
27	2	F	12670
28	1	B	12672
28	2	G	12672
29	1	B	12672
29	2	H	12672
30	1	B	12644
30	2	I	12644
31	1	B	12644
31	2	J	12644
32	1	B	12644
32	2	K	12644
33	1	B	12644
33	2	L	12644
34	1	B	12644
34	2	M	12644
35	1	B	12644
35	2	N	12644
36	1	B	12644
36	2	O	12644
37	1	B	12644
37	2	P	12644
38	1	B	12672
38	2	Q	12672
39	1	B	12672
39	2	R	12672
40	1	B	12672
40	2	S	12672
41	1	B	12672
41	2	T	12672
42	1	B	12644
42	2	U	12644
43	1	B	12644
43	2	V	12644
44	1	B	12644
44	2	W	12644
45	1	B	12644
45	2	X	12644
46	1	C	12674
46	2	D	12674
47	1	C	12674
47	2	E	12674
48	1	C	12670
48	2	F	12670
49	1	C	12672
49	2	G	12672
50	1	C	12672
50	2	H	12672
51	1	C	12646
51	2	I	12646
52	1	C	12644
52	2	J	12644
53	1	C	12646
53	2	K	12646
54	1	C	12644
54	2	L	12644
55	1	C	12644
55	2	M	12644
56	1	C	12644
56	2	N	12644
57	1	C	12644
57	2	O	12644
58	1	C	12644
58	2	P	12644
59	1	C	12672
59	2	Q	12672
60	1	C	12672
60	2	R	12672
61	1	C	12672
61	2	S	12672
62	1	C	12672
62	2	T	12672
63	1	C	12644
63	2	U	12644
64	1	C	12644
64	2	V	12644
65	1	C	12644
65	2	W	12644
66	1	C	12644
66	2	X	12644
67	1	D	12674
67	2	E	12674
68	1	D	12670
68	2	F	12670
69	1	D	12672
69	2	G	12672
70	1	D	12672
70	2	H	12672
71	1	D	12644
71	2	I	12644
72	1	D	12644
72	2	J	12644
73	1	D	12644
73	2	K	12644
74	1	D	12644
74	2	L	12644
75	1	D	12644
75	2	M	12644
76	1	D	12644
76	2	N	12644
77	1	D	12644
77	2	O	12644
78	1	D	12644
78	2	P	12644
79	1	D	12672
79	2	Q	12672
80	1	D	12672
80	2	R	12672
81	1	D	12672
81	2	S	12672
82	1	D	12672
82	2	T	12672
83	1	D	12644
83	2	U	12644
84	1	D	12644
84	2	V	12644
85	1	D	12644
85	2	W	12644
86	1	D	12644
86	2	X	12644
87	1	E	12670
87	2	F	12670
88	1	E	12672
88	2	G	12672
89	1	E	12672
89	2	H	12672
90	1	E	12644
90	2	I	12644
91	1	E	12644
91	2	J	12644
92	1	E	12644
92	2	K	12644
93	1	E	12644
93	2	L	12644
94	1	E	12644
94	2	M	12644
95	1	E	12644
95	2	N	12644
96	1	E	12644
96	2	O	12644
97	1	E	12644
97	2	P	12644
98	1	E	12672
98	2	Q	12672
99	1	E	12672
99	2	R	12672
100	1	E	12672
100	2	S	12672
101	1	E	12672
101	2	T	12672
102	1	E	12644
102	2	U	12644
103	1	E	12644
103	2	V	12644
104	1	E	12644
104	2	W	12644
105	1	E	12644
105	2	X	12644
106	1	F	12670
106	2	G	12670
107	1	F	12670
107	2	H	12670
108	1	F	12642
108	2	I	12642
109	1	F	12642
109	2	J	12642
110	1	F	12642
110	2	K	12642
111	1	F	12642
111	2	L	12642
112	1	F	12642
112	2	M	12642
113	1	F	12642
113	2	N	12642
114	1	F	12642
114	2	O	12642
115	1	F	12642
115	2	P	12642
116	1	F	12670
116	2	Q	12670
117	1	F	12670
117	2	R	12670
118	1	F	12670
118	2	S	12670
119	1	F	12670
119	2	T	12670
120	1	F	12642
120	2	U	12642
121	1	F	12642
121	2	V	12642
122	1	F	12642
122	2	W	12642
123	1	F	12642
123	2	X	12642
124	1	G	12672
124	2	H	12672
125	1	G	12644
125	2	I	12644
126	1	G	12644
126	2	J	12644
127	1	G	12644
127	2	K	12644
128	1	G	12644
128	2	L	12644
129	1	G	12644
129	2	M	12644
130	1	G	12644
130	2	N	12644
131	1	G	12644
131	2	O	12644
132	1	G	12644
132	2	P	12644
133	1	G	12672
133	2	Q	12672
134	1	G	12672
134	2	R	12672
135	1	G	12672
135	2	S	12672
136	1	G	12672
136	2	T	12672
137	1	G	12644
137	2	U	12644
138	1	G	12644
138	2	V	12644
139	1	G	12644
139	2	W	12644
140	1	G	12644
140	2	X	12644
141	1	H	12644
141	2	I	12644
142	1	H	12644
142	2	J	12644
143	1	H	12644
143	2	K	12644
144	1	H	12644
144	2	L	12644
145	1	H	12644
145	2	M	12644
146	1	H	12644
146	2	N	12644
147	1	H	12644
147	2	O	12644
148	1	H	12644
148	2	P	12644
149	1	H	12672
149	2	Q	12672
150	1	H	12672
150	2	R	12672
151	1	H	12672
151	2	S	12672
152	1	H	12672
152	2	T	12672
153	1	H	12644
153	2	U	12644
154	1	H	12644
154	2	V	12644
155	1	H	12644
155	2	W	12644
156	1	H	12644
156	2	X	12644
157	1	I	12644
157	2	J	12644
158	1	I	12646
158	2	K	12646
159	1	I	12644
159	2	L	12644
160	1	I	12644
160	2	M	12644
161	1	I	12644
161	2	N	12644
162	1	I	12644
162	2	O	12644
163	1	I	12644
163	2	P	12644
164	1	I	12644
164	2	Q	12644
165	1	I	12644
165	2	R	12644
166	1	I	12644
166	2	S	12644
167	1	I	12644
167	2	T	12644
168	1	I	12644
168	2	U	12644
169	1	I	12644
169	2	V	12644
170	1	I	12644
170	2	W	12644
171	1	I	12644
171	2	X	12644
172	1	J	12644
172	2	K	12644
173	1	J	12644
173	2	L	12644
174	1	J	12644
174	2	M	12644
175	1	J	12644
175	2	N	12644
176	1	J	12644
176	2	O	12644
177	1	J	12644
177	2	P	12644
178	1	J	12644
178	2	Q	12644
179	1	J	12644
179	2	R	12644
180	1	J	12644
180	2	S	12644
181	1	J	12644
181	2	T	12644
182	1	J	12644
182	2	U	12644
183	1	J	12644
183	2	V	12644
184	1	J	12644
184	2	W	12644
185	1	J	12644
185	2	X	12644
186	1	K	12644
186	2	L	12644
187	1	K	12644
187	2	M	12644
188	1	K	12644
188	2	N	12644
189	1	K	12644
189	2	O	12644
190	1	K	12644
190	2	P	12644
191	1	K	12644
191	2	Q	12644
192	1	K	12644
192	2	R	12644
193	1	K	12644
193	2	S	12644
194	1	K	12644
194	2	T	12644
195	1	K	12644
195	2	U	12644
196	1	K	12644
196	2	V	12644
197	1	K	12644
197	2	W	12644
198	1	K	12644
198	2	X	12644
199	1	L	12644
199	2	M	12644
200	1	L	12644
200	2	N	12644
201	1	L	12644
201	2	O	12644
202	1	L	12644
202	2	P	12644
203	1	L	12644
203	2	Q	12644
204	1	L	12644
204	2	R	12644
205	1	L	12644
205	2	S	12644
206	1	L	12644
206	2	T	12644
207	1	L	12644
207	2	U	12644
208	1	L	12644
208	2	V	12644
209	1	L	12644
209	2	W	12644
210	1	L	12644
210	2	X	12644
211	1	M	12644
211	2	N	12644
212	1	M	12644
212	2	O	12644
213	1	M	12644
213	2	P	12644
214	1	M	12644
214	2	Q	12644
215	1	M	12644
215	2	R	12644
216	1	M	12644
216	2	S	12644
217	1	M	12644
217	2	T	12644
218	1	M	12644
218	2	U	12644
219	1	M	12644
219	2	V	12644
220	1	M	12644
220	2	W	12644
221	1	M	12644
221	2	X	12644
222	1	N	12644
222	2	O	12644
223	1	N	12644
223	2	P	12644
224	1	N	12644
224	2	Q	12644
225	1	N	12644
225	2	R	12644
226	1	N	12644
226	2	S	12644
227	1	N	12644
227	2	T	12644
228	1	N	12644
228	2	U	12644
229	1	N	12644
229	2	V	12644
230	1	N	12644
230	2	W	12644
231	1	N	12644
231	2	X	12644
232	1	O	12644
232	2	P	12644
233	1	O	12644
233	2	Q	12644
234	1	O	12644
234	2	R	12644
235	1	O	12644
235	2	S	12644
236	1	O	12644
236	2	T	12644
237	1	O	12644
237	2	U	12644
238	1	O	12644
238	2	V	12644
239	1	O	12644
239	2	W	12644
240	1	O	12644
240	2	X	12644
241	1	P	12644
241	2	Q	12644
242	1	P	12644
242	2	R	12644
243	1	P	12644
243	2	S	12644
244	1	P	12644
244	2	T	12644
245	1	P	12644
245	2	U	12644
246	1	P	12644
246	2	V	12644
247	1	P	12644
247	2	W	12644
248	1	P	12644
248	2	X	12644
249	1	Q	12672
249	2	R	12672
250	1	Q	12672
250	2	S	12672
251	1	Q	12672
251	2	T	12672
252	1	Q	12644
252	2	U	12644
253	1	Q	12644
253	2	V	12644
254	1	Q	12644
254	2	W	12644
255	1	Q	12644
255	2	X	12644
256	1	R	12672
256	2	S	12672
257	1	R	12672
257	2	T	12672
258	1	R	12644
258	2	U	12644
259	1	R	12644
259	2	V	12644
260	1	R	12644
260	2	W	12644
261	1	R	12644
261	2	X	12644
262	1	S	12672
262	2	T	12672
263	1	S	12644
263	2	U	12644
264	1	S	12644
264	2	V	12644
265	1	S	12644
265	2	W	12644
266	1	S	12644
266	2	X	12644
267	1	T	12644
267	2	U	12644
268	1	T	12644
268	2	V	12644
269	1	T	12644
269	2	W	12644
270	1	T	12644
270	2	X	12644
271	1	U	12644
271	2	V	12644
272	1	U	12644
272	2	W	12644
273	1	U	12644
273	2	X	12644
274	1	V	12644
274	2	W	12644
275	1	V	12644
275	2	X	12644
276	1	W	12642
276	2	X	12642

LS精密化 シェル

解像度: 1.6→1.642 Å / Total num. of bins used: 20

	Rfactor	反射数	%反射
Rfree	0	0	-
Rwork	0.687	87955	-
obs	-	-	100 %