Journal: J Virol / Year: 2021 Title: Capsid Structure of Cyanophage A-1(L). Authors: Ning Cui / Feng Yang / Jun-Tao Zhang / Hui Sun / Yu Chen / Rong-Cheng Yu / Zhi-Peng Chen / Yong-Liang Jiang / Shu-Jing Han / Xudong Xu / Qiong Li / Cong-Zhao Zhou / Abstract: A-1(L) is a freshwater cyanophage with a contractile tail that specifically infects sp. PCC 7120, one of the model strains for molecular studies of cyanobacteria. Although isolated for half a ...A-1(L) is a freshwater cyanophage with a contractile tail that specifically infects sp. PCC 7120, one of the model strains for molecular studies of cyanobacteria. Although isolated for half a century, its structure remains unknown, which limits our understanding on the interplay between A-1(L) and its host. Here we report the 3.35 Å cryo-EM structure of A-1(L) capsid, representing the first near-atomic resolution structure of a phage capsid with a T number of 9. The major capsid gp4 proteins assemble into 91 capsomers, including 80 hexons: 20 at the center of the facet and 60 at the facet edge, in addition to 11 identical pentons. These capsomers further assemble into the icosahedral capsid, via gradually increasing curvatures. Different from the previously reported capsids of known-structure, A-1(L) adopts a noncovalent chainmail structure of capsid stabilized by two kinds of mortise-and-tenon inter-capsomer interactions: a three-layered interface at the pseudo 3-fold axis combined with the complementarity in shape and electrostatic potential around the 2-fold axis. This unique capsomer construction enables A-1(L) to possess a rigid capsid, which is solely composed of the major capsid proteins with an HK97 fold. Cyanobacteria are the most abundant photosynthetic bacteria, contributing significantly to the biomass production, O generation, and CO consumption on our planet. Their community structure and homeostasis in natural aquatic ecosystems are largely regulated by the corresponding cyanophages. In this study, we solved the structure of cyanophage A-1(L) capsid at near-atomic resolution and revealed a unique capsid construction. This capsid structure provides the molecular details for better understanding the assembly of A-1(L), and a structural platform for future investigation and application of A-1(L) in combination with its host sp. PCC 7120. As the first isolated freshwater cyanophage that infects the genetically tractable model cyanobacterium, A-1(L) should become an ideal template for the genetic engineering and synthetic biology studies.
A: Putative major capsid protein B: Putative major capsid protein C: Putative major capsid protein D: Putative major capsid protein E: Putative major capsid protein F: Putative major capsid protein G: Putative major capsid protein H: Putative major capsid protein I: Putative major capsid protein
A: Putative major capsid protein B: Putative major capsid protein C: Putative major capsid protein D: Putative major capsid protein E: Putative major capsid protein F: Putative major capsid protein G: Putative major capsid protein H: Putative major capsid protein I: Putative major capsid protein
A: Putative major capsid protein B: Putative major capsid protein C: Putative major capsid protein D: Putative major capsid protein E: Putative major capsid protein F: Putative major capsid protein G: Putative major capsid protein H: Putative major capsid protein I: Putative major capsid protein
x 5
icosahedral pentamer
1.78 MDa, 45 polymers
Theoretical mass
Number of molelcules
Total (without water)
1,778,608
45
Polymers
1,778,608
45
Non-polymers
0
0
Water
0
Type
Name
Symmetry operation
Number
identity operation
1_555
x,y,z
1
point symmetry operation
4
4
A: Putative major capsid protein B: Putative major capsid protein C: Putative major capsid protein D: Putative major capsid protein E: Putative major capsid protein F: Putative major capsid protein G: Putative major capsid protein H: Putative major capsid protein I: Putative major capsid protein
x 6
icosahedral 23 hexamer
2.13 MDa, 54 polymers
Theoretical mass
Number of molelcules
Total (without water)
2,134,329
54
Polymers
2,134,329
54
Non-polymers
0
0
Water
0
Type
Name
Symmetry operation
Number
identity operation
1_555
x,y,z
1
point symmetry operation
5
5
Idetical with deposited unit in distinct coordinate
icosahedral asymmetric unit, std point frame
Type
Name
Symmetry operation
Number
transform to point frame
1
Symmetry
Point symmetry: (Schoenflies symbol: I (icosahedral))
-
Components
#1: Protein
Putativemajorcapsidprotein
Mass: 39524.617 Da / Num. of mol.: 9 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Nostoc phage A1 (virus) / Production host: Nostoc phage A1 (virus) / References: UniProt: A0A191SAV5
-
Experimental details
-
Experiment
Experiment
Method: ELECTRON MICROSCOPY
EM experiment
Aggregation state: PARTICLE / 3D reconstruction method: single particle reconstruction
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