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基本情報
登録情報 | データベース: PDB / ID: 7.0E+20 | |||||||||
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タイトル | Cryo EM structure of a K+-bound Na+,K+-ATPase in the E2 state | |||||||||
![]() | (Sodium/potassium-transporting ATPase subunit ...) x 3 | |||||||||
![]() | MEMBRANE PROTEIN / Na+ / K+-ATPase | |||||||||
機能・相同性 | ![]() negative regulation of glucocorticoid biosynthetic process / protein transport into plasma membrane raft / positive regulation of calcium:sodium antiporter activity / positive regulation of heart contraction / positive regulation of potassium ion transmembrane transporter activity / positive regulation of P-type sodium:potassium-exchanging transporter activity / Na+/K+-exchanging ATPase / positive regulation of striated muscle contraction / positive regulation of sodium ion export across plasma membrane / positive regulation of potassium ion import across plasma membrane ...negative regulation of glucocorticoid biosynthetic process / protein transport into plasma membrane raft / positive regulation of calcium:sodium antiporter activity / positive regulation of heart contraction / positive regulation of potassium ion transmembrane transporter activity / positive regulation of P-type sodium:potassium-exchanging transporter activity / Na+/K+-exchanging ATPase / positive regulation of striated muscle contraction / positive regulation of sodium ion export across plasma membrane / positive regulation of potassium ion import across plasma membrane / response to glycoside / membrane repolarization during cardiac muscle cell action potential / photoreceptor inner segment membrane / P-type sodium:potassium-exchanging transporter activity / steroid hormone binding / membrane repolarization / sodium ion binding / sodium:potassium-exchanging ATPase complex / negative regulation of heart contraction / establishment or maintenance of transmembrane electrochemical gradient / regulation of the force of heart contraction / osmosensory signaling pathway / sodium ion export across plasma membrane / cell communication by electrical coupling involved in cardiac conduction / intracellular potassium ion homeostasis / intracellular sodium ion homeostasis / cardiac muscle cell action potential involved in contraction / positive regulation of ATP-dependent activity / regulation of cardiac muscle contraction by calcium ion signaling / relaxation of cardiac muscle / Basigin interactions / cellular response to steroid hormone stimulus / organelle membrane / potassium ion import across plasma membrane / phosphatase activity / monoatomic cation transmembrane transport / potassium ion binding / Ion transport by P-type ATPases / ATPase activator activity / intercalated disc / lateral plasma membrane / sperm flagellum / sodium ion transmembrane transport / sodium channel regulator activity / cardiac muscle contraction / ATP metabolic process / regulation of sodium ion transport / Ion homeostasis / T-tubule / proton transmembrane transport / protein localization to plasma membrane / regulation of blood pressure / sarcolemma / transmembrane transport / intracellular calcium ion homeostasis / extracellular vesicle / melanosome / MHC class II protein complex binding / ATPase binding / regulation of gene expression / protein-macromolecule adaptor activity / protein-folding chaperone binding / basolateral plasma membrane / Potential therapeutics for SARS / transmembrane transporter binding / postsynaptic density / protein stabilization / cell adhesion / apical plasma membrane / response to xenobiotic stimulus / membrane raft / protein heterodimerization activity / axon / innate immune response / protein kinase binding / Golgi apparatus / endoplasmic reticulum / ATP hydrolysis activity / protein-containing complex / extracellular exosome / ATP binding / membrane / plasma membrane 類似検索 - 分子機能 | |||||||||
生物種 | ![]() | |||||||||
手法 | 電子顕微鏡法 / 単粒子再構成法 / クライオ電子顕微鏡法 / 解像度: 2.7 Å | |||||||||
![]() | Guo, Y.Y. / Zhang, Y.Y. / Yan, R.H. / Huang, B.D. / Ye, F.F. / Wu, L.S. / Chi, X.M. / Zhou, Q. | |||||||||
資金援助 | ![]()
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![]() | ![]() タイトル: Cryo-EM structures of recombinant human sodium-potassium pump determined in three different states. 著者: Yingying Guo / Yuanyuan Zhang / Renhong Yan / Bangdong Huang / Fangfei Ye / Liushu Wu / Ximin Chi / Yi Shi / Qiang Zhou / ![]() 要旨: Sodium-Potassium Pump (Na/K-ATPase, NKA) is an ion pump that generates an electrochemical gradient of sodium and potassium ions across the plasma membrane by hydrolyzing ATP. During each Post-Albers ...Sodium-Potassium Pump (Na/K-ATPase, NKA) is an ion pump that generates an electrochemical gradient of sodium and potassium ions across the plasma membrane by hydrolyzing ATP. During each Post-Albers cycle, NKA exchanges three cytoplasmic sodium ions for two extracellular potassium ions through alternating changes between the E1 and E2 states. Hitherto, several steps remained unknown during the complete working cycle of NKA. Here, we report cryo-electron microscopy (cryo-EM) structures of recombinant human NKA (hNKA) in three distinct states at 2.7-3.2 Å resolution, representing the E1·3Na and E1·3Na·ATP states with cytosolic gates open and the basic E2·[2K] state, respectively. This work provides the insights into the cytoplasmic Na entrance pathway and the mechanism of cytoplasmic gate closure coupled with ATP hydrolysis, filling crucial gaps in the structural elucidation of the Post-Albers cycle of NKA. | |||||||||
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構造の表示
構造ビューア | 分子: ![]() ![]() |
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PDBx/mmCIF形式 | ![]() | 249.1 KB | 表示 | ![]() |
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PDB形式 | ![]() | 197.8 KB | 表示 | ![]() |
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-検証レポート
文書・要旨 | ![]() | 1.3 MB | 表示 | ![]() |
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文書・詳細版 | ![]() | 1.3 MB | 表示 | |
XML形式データ | ![]() | 47.2 KB | 表示 | |
CIF形式データ | ![]() | 67.7 KB | 表示 | |
アーカイブディレクトリ | ![]() ![]() | HTTPS FTP |
-関連構造データ
関連構造データ | ![]() 30948MC ![]() 7e1zC ![]() 7e21C M: このデータのモデリングに利用したマップデータ C: 同じ文献を引用 ( |
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類似構造データ | 類似検索 - 機能・相同性 ![]() |
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リンク
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集合体
登録構造単位 | ![]()
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要素
-Sodium/potassium-transporting ATPase subunit ... , 3種, 3分子 ABC
#1: タンパク質 | 分子量: 113012.828 Da / 分子数: 1 / 由来タイプ: 組換発現 / 由来: (組換発現) ![]() ![]() |
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#2: タンパク質 | 分子量: 35108.258 Da / 分子数: 1 / 由来タイプ: 組換発現 / 由来: (組換発現) ![]() ![]() |
#3: タンパク質 | 分子量: 7292.322 Da / 分子数: 1 / 由来タイプ: 組換発現 / 由来: (組換発現) ![]() ![]() |
-糖 , 2種, 2分子 ![](data/chem/img/NAG.gif)
#4: 多糖 | 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose |
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#9: 糖 | ChemComp-NAG / |
-非ポリマー , 5種, 12分子 ![](data/chem/img/K.gif)
![](data/chem/img/MG.gif)
![](data/chem/img/Y01.gif)
![](data/chem/img/PC1.gif)
![](data/chem/img/HOH.gif)
![](data/chem/img/MG.gif)
![](data/chem/img/Y01.gif)
![](data/chem/img/PC1.gif)
![](data/chem/img/HOH.gif)
#5: 化合物 | #6: 化合物 | ChemComp-MG / | #7: 化合物 | ChemComp-Y01 / #8: 化合物 | ChemComp-PC1 / | #10: 水 | ChemComp-HOH / | |
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-詳細
研究の焦点であるリガンドがあるか | Y |
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-実験情報
-実験
実験 | 手法: 電子顕微鏡法 |
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EM実験 | 試料の集合状態: PARTICLE / 3次元再構成法: 単粒子再構成法 |
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試料調製
構成要素 | 名称: cryo EM structure of a K+-bound Na+,K+-ATPase in the E2 state タイプ: COMPLEX / Entity ID: #1-#3 / 由来: RECOMBINANT |
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由来(天然) | 生物種: ![]() |
由来(組換発現) | 生物種: ![]() |
緩衝液 | pH: 8 |
試料 | 包埋: NO / シャドウイング: NO / 染色: NO / 凍結: YES |
急速凍結 | 凍結剤: ETHANE |
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電子顕微鏡撮影
実験機器 | ![]() モデル: Titan Krios / 画像提供: FEI Company |
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顕微鏡 | モデル: FEI TITAN KRIOS |
電子銃 | 電子線源: ![]() |
電子レンズ | モード: BRIGHT FIELD / 最大 デフォーカス(公称値): 2200 nm / 最小 デフォーカス(公称値): 1200 nm / アライメント法: COMA FREE |
試料ホルダ | 凍結剤: NITROGEN 試料ホルダーモデル: FEI TITAN KRIOS AUTOGRID HOLDER |
撮影 | 電子線照射量: 50 e/Å2 フィルム・検出器のモデル: GATAN K3 BIOQUANTUM (6k x 4k) |
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解析
EMソフトウェア | 名称: RELION / バージョン: 3.0.6 / カテゴリ: 3次元再構成 |
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CTF補正 | タイプ: PHASE FLIPPING AND AMPLITUDE CORRECTION |
3次元再構成 | 解像度: 2.7 Å / 解像度の算出法: FSC 0.143 CUT-OFF / 粒子像の数: 53681 / 対称性のタイプ: POINT |