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- PDB-7a4p: Structure of small high-light grown Chlorella ohadii photosystem I -
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Basic information
Entry | Database: PDB / ID: 7a4p | |||||||||
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Title | Structure of small high-light grown Chlorella ohadii photosystem I | |||||||||
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![]() | PHOTOSYNTHESIS / photosystem I / light stress | |||||||||
Function / homology | ![]() malate transport / chloroplast thylakoid lumen / photosynthesis, light harvesting in photosystem I / glutathione-disulfide reductase (NADPH) activity / photosystem I reaction center / photosystem I / photosystem I / oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen / photosystem II / acyltransferase activity, transferring groups other than amino-acyl groups ...malate transport / chloroplast thylakoid lumen / photosynthesis, light harvesting in photosystem I / glutathione-disulfide reductase (NADPH) activity / photosystem I reaction center / photosystem I / photosystem I / oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen / photosystem II / acyltransferase activity, transferring groups other than amino-acyl groups / chlorophyll binding / photosynthetic electron transport in photosystem I / chloroplast thylakoid membrane / response to light stimulus / photosynthesis / monooxygenase activity / glutathione metabolic process / cell redox homeostasis / chloroplast / flavin adenine dinucleotide binding / 4 iron, 4 sulfur cluster binding / cellular response to oxidative stress / oxidoreductase activity / electron transfer activity / iron ion binding / heme binding / magnesium ion binding / mitochondrion / membrane / metal ion binding / cytosol Similarity search - Function | |||||||||
Biological species | ![]() | |||||||||
Method | ELECTRON MICROSCOPY / single particle reconstruction / cryo EM / Resolution: 4.2 Å | |||||||||
![]() | Caspy, I. / Nelson, N. / Nechushtai, R. / Shkolnisky, Y. / Neumann, E. | |||||||||
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![]() | ![]() Title: Cryo-EM photosystem I structure reveals adaptation mechanisms to extreme high light in Chlorella ohadii. Authors: Ido Caspy / Ehud Neumann / Maria Fadeeva / Varda Liveanu / Anton Savitsky / Anna Frank / Yael Levi Kalisman / Yoel Shkolnisky / Omer Murik / Haim Treves / Volker Hartmann / Marc M Nowaczyk / ...Authors: Ido Caspy / Ehud Neumann / Maria Fadeeva / Varda Liveanu / Anton Savitsky / Anna Frank / Yael Levi Kalisman / Yoel Shkolnisky / Omer Murik / Haim Treves / Volker Hartmann / Marc M Nowaczyk / Wolfgang Schuhmann / Matthias Rögner / Itamar Willner / Aaron Kaplan / Gadi Schuster / Nathan Nelson / Wolfgang Lubitz / Rachel Nechushtai / ![]() ![]() Abstract: Photosynthesis in deserts is challenging since it requires fast adaptation to rapid night-to-day changes, that is, from dawn's low light (LL) to extreme high light (HL) intensities during the daytime. ...Photosynthesis in deserts is challenging since it requires fast adaptation to rapid night-to-day changes, that is, from dawn's low light (LL) to extreme high light (HL) intensities during the daytime. To understand these adaptation mechanisms, we purified photosystem I (PSI) from Chlorella ohadii, a green alga that was isolated from a desert soil crust, and identified the essential functional and structural changes that enable the photosystem to perform photosynthesis under extreme high light conditions. The cryo-electron microscopy structures of PSI from cells grown under low light (PSI) and high light (PSI), obtained at 2.70 and 2.71 Å, respectively, show that part of light-harvesting antenna complex I (LHCI) and the core complex subunit (PsaO) are eliminated from PSI to minimize the photodamage. An additional change is in the pigment composition and their number in LHCI; about 50% of chlorophyll b is replaced by chlorophyll a. This leads to higher electron transfer rates in PSI and might enable C. ohadii PSI to act as a natural photosynthesiser in photobiocatalytic systems. PSI or PSI were attached to an electrode and their induced photocurrent was determined. To obtain photocurrents comparable with PSI, 25 times the amount of PSI was required, demonstrating the high efficiency of PSI. Hence, we suggest that C. ohadii PSI is an ideal candidate for the design of desert artificial photobiocatalytic systems. | |||||||||
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PDBx/mmCIF format | ![]() | 1.1 MB | Display | ![]() |
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PDB format | ![]() | 981.5 KB | Display | ![]() |
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-Validation report
Summary document | ![]() | 15.5 MB | Display | ![]() |
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Full document | ![]() | 16.6 MB | Display | |
Data in XML | ![]() | 349.3 KB | Display | |
Data in CIF | ![]() | 408.8 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 11640MC ![]() 6zzxC ![]() 6zzyC M: map data used to model this data C: citing same article ( |
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Similar structure data |
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Assembly
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Components
-Photosystem I P700 chlorophyll a apoprotein ... , 2 types, 2 molecules AB
#1: Protein | Mass: 82125.898 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) ![]() |
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#2: Protein | Mass: 81544.633 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) ![]() |
-Protein , 5 types, 5 molecules CFKL3
#3: Protein | Mass: 8723.009 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) ![]() |
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#6: Protein | Mass: 18069.887 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) ![]() |
#9: Protein | Mass: 8725.087 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) ![]() |
#12: Protein | Mass: 16501.025 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) ![]() |
#14: Protein | Mass: 26163.904 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) ![]() |
-Photosystem I reaction center subunit ... , 7 types, 7 molecules DEGJMIH
#4: Protein | Mass: 15958.232 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) ![]() |
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#5: Protein | Mass: 7194.105 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) ![]() |
#7: Protein | Mass: 10326.872 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) ![]() |
#8: Protein/peptide | Mass: 4475.292 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) ![]() |
#10: Protein/peptide | Mass: 3376.060 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) ![]() |
#11: Protein/peptide | Mass: 3856.674 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) ![]() |
#20: Protein/peptide | Mass: 329.392 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) ![]() |
-Chlorophyll a-b binding protein, ... , 6 types, 7 molecules 1a45678
#13: Protein | Mass: 19933.604 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) ![]() #15: Protein | | Mass: 23010.994 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) ![]() #16: Protein | | Mass: 25111.686 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) ![]() #17: Protein | | Mass: 25316.240 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) ![]() #18: Protein | | Mass: 24016.156 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) ![]() #19: Protein | | Mass: 23612.713 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) ![]() |
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-Sugars , 2 types, 6 molecules ![](data/chem/img/DGD.gif)
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#26: Sugar | #29: Sugar | |
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+Non-polymers , 27 types, 331 molecules ![](data/chem/img/CL0.gif)
![](data/chem/img/CLA.gif)
![](data/chem/img/PQN.gif)
![](data/chem/img/BCR.gif)
![](data/chem/img/LHG.gif)
![](data/chem/img/3PH.gif)
![](data/chem/img/SF4.gif)
![](data/chem/img/PCW.gif)
![](data/chem/img/PTY.gif)
![](data/chem/img/LAP.gif)
![](data/chem/img/SQD.gif)
![](data/chem/img/ERG.gif)
![](data/chem/img/RRX.gif)
![](data/chem/img/LPX.gif)
![](data/chem/img/ECH.gif)
![](data/chem/img/LUT.gif)
![](data/chem/img/CHL.gif)
![](data/chem/img/OLA.gif)
![](data/chem/img/QTB.gif)
![](data/chem/img/GG0.gif)
![](data/chem/img/PLM.gif)
![](data/chem/img/DGA.gif)
![](data/chem/img/SPH.gif)
![](data/chem/img/XAT.gif)
![](data/chem/img/4RF.gif)
![](data/chem/img/C7Z.gif)
![](data/chem/img/P5S.gif)
![](data/chem/img/CLA.gif)
![](data/chem/img/PQN.gif)
![](data/chem/img/BCR.gif)
![](data/chem/img/LHG.gif)
![](data/chem/img/3PH.gif)
![](data/chem/img/SF4.gif)
![](data/chem/img/PCW.gif)
![](data/chem/img/PTY.gif)
![](data/chem/img/LAP.gif)
![](data/chem/img/SQD.gif)
![](data/chem/img/ERG.gif)
![](data/chem/img/RRX.gif)
![](data/chem/img/LPX.gif)
![](data/chem/img/ECH.gif)
![](data/chem/img/LUT.gif)
![](data/chem/img/CHL.gif)
![](data/chem/img/OLA.gif)
![](data/chem/img/QTB.gif)
![](data/chem/img/GG0.gif)
![](data/chem/img/PLM.gif)
![](data/chem/img/DGA.gif)
![](data/chem/img/SPH.gif)
![](data/chem/img/XAT.gif)
![](data/chem/img/4RF.gif)
![](data/chem/img/C7Z.gif)
![](data/chem/img/P5S.gif)