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基本情報
登録情報 | データベース: PDB / ID: 6l7a | ||||||
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タイトル | CsgFG complex in Curli biogenesis system | ||||||
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![]() | PROTEIN TRANSPORT / Biofilm / Curli biogenesis system / CsgFG complex / protein transportation | ||||||
機能・相同性 | ![]() curli secretion complex / curli assembly / protein secretion by the type VIII secretion system / protein transmembrane transport / single-species biofilm formation / cell outer membrane / outer membrane-bounded periplasmic space / identical protein binding / plasma membrane 類似検索 - 分子機能 | ||||||
生物種 | ![]() ![]() ![]() ![]() | ||||||
手法 | 電子顕微鏡法 / 単粒子再構成法 / クライオ電子顕微鏡法 / 解像度: 3.38 Å | ||||||
![]() | Yan, Z.F. / Yin, M. / Chen, J.N. / Li, X.M. | ||||||
![]() | ![]() タイトル: Assembly and substrate recognition of curli biogenesis system. 著者: Zhaofeng Yan / Meng Yin / Jianan Chen / Xueming Li / ![]() 要旨: A major component of bacterial biofilms is curli amyloid fibrils secreted by the curli biogenesis system. Understanding the curli biogenesis mechanism is critical for developing therapeutic agents ...A major component of bacterial biofilms is curli amyloid fibrils secreted by the curli biogenesis system. Understanding the curli biogenesis mechanism is critical for developing therapeutic agents for biofilm-related infections. Here we report a systematic study of the curli biogenesis system, highlighted by structural, biochemical and functional analysis of the secretion channel complexes (CsgF-CsgG) with and without the curli substrate. The dual-pore architecture of the CsgF-CsgG complex was observed and used to develop an approach to inhibit the curli secretion by physically reducing the size of the CsgF pore. We further elucidated the assembly of the CsgFG complex with curli components (CsgA and CsgB) and curli-cell association through CsgF. Importantly, the recognition of the CsgA substrate by CsgG was uncovered. Nine crevices outside of the CsgG channel provide specific and highly-conserved recognition sites for CsgA N-terminus. Together with analysis of CsgE, our study provides comprehensive insights into curli biogenesis. | ||||||
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集合体
登録構造単位 | ![]()
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要素
#1: タンパク質 | 分子量: 30584.035 Da / 分子数: 9 / 由来タイプ: 組換発現 由来: (組換発現) ![]() ![]() 遺伝子: BX06_26815 / 発現宿主: ![]() ![]() #2: タンパク質 | 分子量: 15065.705 Da / 分子数: 9 / 由来タイプ: 組換発現 / 由来: (組換発現) ![]() ![]() 遺伝子: csgF, A6581_12775, A6592_16410, A8C65_09935, A8G17_17590, A8M42_01085, A9819_06490, A9R57_10355, AC789_1c11470, ACN002_1089, ACN81_09400, ACU57_27055, ACU90_19250, AJ318_00960, AKG99_ ...遺伝子: csgF, A6581_12775, A6592_16410, A8C65_09935, A8G17_17590, A8M42_01085, A9819_06490, A9R57_10355, AC789_1c11470, ACN002_1089, ACN81_09400, ACU57_27055, ACU90_19250, AJ318_00960, AKG99_07850, AM270_13185, AM464_23205, AMK83_16440, AML07_18510, APU18_12970, APZ14_01615, ARC77_26185, AU473_14305, AUQ13_23570, AUS26_14005, AW106_27240, AWE53_020770, AWF59_023705, AWG78_002955, AWP75_16905, AZZ83_000749, B1K96_25200, B7C53_12210, B9T59_24180, BANRA_01291, BANRA_01576, BANRA_01635, BANRA_04608, BB545_06045, BER14_09940, BHS81_06565, BHS87_05490, BIQ87_05800, BIU72_07215, BIZ41_17475, BJJ90_16050, BK292_02235, BK334_17375, BK373_20095, BK400_19610, BMT49_08095, BMT91_12220, BN17_09001, BON63_21540, BON66_00235, BON69_23620, BON76_26665, BON92_25375, BON93_01750, BTQ04_04830, BTQ06_28615, BUE81_01495, BvCms12BK_01199, BvCms2454_01967, BvCms35BK_05180, BvCmsA75A_03631, BvCmsF63A_02901, BvCmsH15A_03205, BvCmsHHP001_05125, BvCmsHHP019_01648, BvCmsHHP056_03328, BvCmsJ76A_01745, BvCmsKKP061_02124, BvCmsKSNP019_00536, BvCmsKSNP073_05115, BvCmsKSNP081_02155, BvCmsKSNP120_02370, BvCmsKSP008_00766, BvCmsKSP011_05236, BvCmsKSP015_04950, BvCmsKSP018_00729, BvCmsKSP024_02020, BvCmsKSP026_03242, BvCmsKSP036_02551, BvCmsKSP039_03584, BvCmsKSP040_00078, BvCmsKSP045_01294, BvCmsKSP054_01494, BvCmsKSP058_01099, BvCmsKSP061_05085, BvCmsKSP067_02664, BvCmsKSP076_00221, BvCmsKSP081_01991, BvCmsKSP083_04126, BvCmsNSNP006_00926, BvCmsNSNP023_01666, BvCmsNSNP027_01041, BvCmsNSNP036_01556, BvCmsNSP006_01448, BvCmsNSP007_03010, BvCmsNSP039_04491, BvCmsNSP045_01873, BvCmsNSP047_03188, BvCmsNSP052_01638, BvCmsNSP072_01737, BvCmsNSP078_01753, BvCmsOUP014_03127, BvCmsSINP011_02870, BvCmsSINP022_01393, BvCmsSINP036_02015, BvCmsSIP006_02579, BvCmsSIP010_04114, BvCmsSIP019_05011, BvCmsSIP044_01602, BVL39_23020, BW690_20185, BWP17_14750, C1I57_23660, C2M16_23325, C2U48_03735, C3449_22250, C4J69_16245, C4K41_10685, C5N07_21975, C5P01_13585, C5P43_18785, C5P44_04330, C6669_24200, C6986_06430, C6B13_08320, C7235_15270, C7B02_01110, C7B06_10030, C7B07_08350, C9025_13220, C9083_13050, C9200_05635, C9212_12645, C9255_16880, C9299_13245, C9E25_15565, C9E67_18455, C9Y80_08970, C9Y95_14355, C9Z12_15420, CA593_23315, CDL37_22840, CG692_25470, CI641_021185, CI694_24070, COD30_07495, COD46_11805, CQP61_11360, CR538_15905, CR539_09835, CRD98_09770, CRE06_18275, CRM83_09165, CSB64_07125, CT143_19775, CT146_19235, CVH05_20310, CWM24_09415, CWS33_22375, D0X26_23320, D1900_15070, D1912_33520, D2183_15675, D2184_16790, D2185_03375, D2F89_16725, D3821_20245, D3822_19530, D3C88_18190, D3O91_17020, D3Y67_15515, D4M06_16745, D7K63_17880, D7K66_17230, D7Y10_25390, D9D20_13510, D9E35_09110, D9G11_01800, D9G42_02010, D9H53_22330, D9H70_23200, D9H94_04555, D9I11_06735, D9I18_09120, D9I20_14875, D9I87_24110, D9I97_06115, D9J03_24045, D9J11_10875, D9J44_05170, D9K48_17895, D9K54_26755, D9L89_17300, D9L99_25015, D9X97_17120, DAH26_20385, DAH27_16630, DAH36_09625, DAH37_07515, DEN86_26015, DEN89_04050, DEO11_03635, DEO19_16190, DIV22_28760, DL455_07100, DL545_15180, DL800_08370, DMI04_15810, DNB37_24555, DNQ41_09265, DNR41_04640, DOY56_16760, DP258_00480, DQE83_00815, DQF57_17655, DQO13_15190, DS732_10230, DS966_20145, DTL43_16545, DTL90_00105, DTM10_22410, DTM25_02505, DU333_10225, DW236_07305, DXT71_23350, E0E06_19720, E2119_27400, E2132_08845, E2855_01310, E2863_01207, E5M00_13230, EAI42_03980, EAI46_03030, EAI52_09335, EB509_13100, EB510_15335, EB515_17370, EC3234A_26c00110, EC3426_01949, EC95NR1_05434, ECONIH1_06410, ECs1415, ECTO6_02921, ED225_16610, ED600_22300, ED607_11755, ED611_18205, ED648_24045, ED903_22580, ED944_18280, EEA45_10640, EEP23_24640, EF173_18855, EFV06_07775, EFV08_14150, EGY17_18935, EIA08_02665, EIA21_07755, EJC75_04035, EJH97_14750, EKI52_04095, EL75_2704, EL79_2739, EL80_2716, ELT33_12235, EO241_06505, EPS94_06625, EPS97_23230, EQ825_00060, ERL57_04175, ERS085365_03587, ERS085379_02716, ERS085383_03172, ERS085404_02838, ERS085406_01151, ERS085416_00210, ERS139211_03440, ERS150873_02816, ERS150876_03011, EVY21_12440, EWK56_19810, EXM29_11820, ExPECSC022_03109, ExPECSC038_03439, EXX06_01125, EXX13_00845, EXX23_16015, EXX24_14295, EXX53_14790, EXX55_01320, EXX71_17360, EXX78_02225, EXX87_08890, EYY78_26035, GJ11_06285, HmCms184_02447, HmCmsJML079_02982, HmCmsJML146_03699, HmCmsJML204_00878, HW43_08900, JD73_25430, MJ49_00980, MS6198_11710, MS8345_01023, NCTC10418_04583, NCTC10429_05442, NCTC10766_04503, NCTC10767_04990, NCTC11022_00191, NCTC11126_00990, NCTC11341_04818, NCTC13127_04125, NCTC13462_01067, NCTC7922_00566, NCTC7927_03442, NCTC8009_05720, NCTC8179_02810, NCTC8622_05879, NCTC8960_00713, NCTC8985_01811, NCTC9007_01932, NCTC9036_03192, NCTC9045_03609, NCTC9055_04891, NCTC9058_03886, NCTC9062_05238, NCTC9075_04088, NCTC9111_03151, NCTC9119_03148, NCTC9434_03595, NCTC9701_03328, NCTC9703_02363, NCTC9706_00463, NCTC9777_03104, NCTC9962_01914, NCTC9969_03241, PU06_15680, RG28_16620, RK56_002580, RX35_02724, SAMEA3472043_03793, SAMEA3472044_05359, SAMEA3472047_01444, SAMEA3472055_02534, SAMEA3472056_01744, SAMEA3472070_03172, SAMEA3472080_02978, SAMEA3472090_04886, SAMEA3472108_02247, SAMEA3472114_01584, SAMEA3472147_02312, SAMEA3484427_03684, SAMEA3484429_03764, SAMEA3485101_00247, SAMEA3485113_04376, SAMEA3752553_01957, SAMEA3752557_01505, SAMEA3752559_02004, SAMEA3752620_04473, SAMEA3753064_00899, SAMEA3753097_05333, SAMEA3753164_01770, SAMEA3753290_02723, SK85_01106, UN86_07965, UN91_14220, WQ89_17310, WR15_23585 発現宿主: ![]() ![]() |
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-実験情報
-実験
実験 | 手法: 電子顕微鏡法 |
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EM実験 | 試料の集合状態: PARTICLE / 3次元再構成法: 単粒子再構成法 |
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試料調製
構成要素 | 名称: Curli transport CsgFG complex / タイプ: COMPLEX / Entity ID: all / 由来: RECOMBINANT |
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由来(天然) | 生物種: ![]() ![]() |
由来(組換発現) | 生物種: ![]() ![]() |
緩衝液 | pH: 8 |
試料 | 包埋: NO / シャドウイング: NO / 染色: NO / 凍結: YES |
急速凍結 | 凍結剤: ETHANE |
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電子顕微鏡撮影
顕微鏡 | モデル: FEI/PHILIPS CM300FEG/HE |
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電子銃 | 電子線源: ![]() |
電子レンズ | モード: BRIGHT FIELD |
撮影 | 電子線照射量: 50 e/Å2 フィルム・検出器のモデル: GATAN K2 SUMMIT (4k x 4k) |
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解析
CTF補正 | タイプ: NONE |
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3次元再構成 | 解像度: 3.38 Å / 解像度の算出法: FSC 0.143 CUT-OFF / 粒子像の数: 372680 / 対称性のタイプ: POINT |