regulation of synaptic vesicle docking / glutamatergic postsynaptic density / HSF1-dependent transactivation / RAF activation / Ion transport by P-type ATPases / peptidyl-threonine autophosphorylation / regulation of endocannabinoid signaling pathway / neurotransmitter receptor transport to plasma membrane / : / calcium- and calmodulin-dependent protein kinase complex ...regulation of synaptic vesicle docking / glutamatergic postsynaptic density / HSF1-dependent transactivation / RAF activation / Ion transport by P-type ATPases / peptidyl-threonine autophosphorylation / regulation of endocannabinoid signaling pathway / neurotransmitter receptor transport to plasma membrane / : / calcium- and calmodulin-dependent protein kinase complex / Interferon gamma signaling / Ca2+/calmodulin-dependent protein kinase / regulation of neurotransmitter secretion / regulation of neuron migration / dendritic spine development / calcium-dependent protein serine/threonine kinase activity / Trafficking of AMPA receptors / positive regulation of calcium ion transport / Ca2+ pathway / negative regulation of hydrolase activity / postsynaptic neurotransmitter receptor diffusion trapping / presynaptic cytosol / GTPase activating protein binding / postsynaptic specialization membrane / RAF/MAP kinase cascade / dendrite morphogenesis / NMDA selective glutamate receptor signaling pathway / regulation of mitochondrial membrane permeability involved in apoptotic process / regulation of neurotransmitter receptor localization to postsynaptic specialization membrane / calcium/calmodulin-dependent protein kinase activity / postsynaptic cytosol / Ion homeostasis / positive regulation of cardiac muscle cell apoptotic process / regulation of neuronal synaptic plasticity / cellular response to interferon-beta / regulation of protein localization to plasma membrane / Unblocking of NMDA receptors, glutamate binding and activation / glutamate receptor binding / ionotropic glutamate receptor signaling pathway / dendrite cytoplasm / response to ischemia / angiotensin-activated signaling pathway / positive regulation of receptor signaling pathway via JAK-STAT / Schaffer collateral - CA1 synapse / cellular response to type II interferon / G1/S transition of mitotic cell cycle / calcium ion transport / kinase activity / dendritic spine / postsynaptic density / calmodulin binding / neuron projection / axon / protein phosphorylation / protein serine kinase activity / protein serine/threonine kinase activity / neuronal cell body / glutamatergic synapse / synapse / dendrite / protein homodimerization activity / mitochondrion / ATP binding / identical protein binding / metal ion binding / cytoplasm / cytosol 類似検索 - 分子機能
Calcium/calmodulin-dependent protein kinase II, association-domain / Calcium/calmodulin dependent protein kinase II association domain / NTF2-like domain superfamily / Serine/threonine-protein kinase, active site / Serine/Threonine protein kinases active-site signature. / Protein kinase domain / Serine/Threonine protein kinases, catalytic domain / Protein kinase, ATP binding site / Protein kinases ATP-binding region signature. / Protein kinase domain profile. ...Calcium/calmodulin-dependent protein kinase II, association-domain / Calcium/calmodulin dependent protein kinase II association domain / NTF2-like domain superfamily / Serine/threonine-protein kinase, active site / Serine/Threonine protein kinases active-site signature. / Protein kinase domain / Serine/Threonine protein kinases, catalytic domain / Protein kinase, ATP binding site / Protein kinases ATP-binding region signature. / Protein kinase domain profile. / Protein kinase domain / Protein kinase-like domain superfamily 類似検索 - ドメイン・相同性
Calcium/calmodulin-dependent protein kinase type II subunit alpha 類似検索 - 構成要素
National Institutes of Health/National Institute of Neurological Disorders and Stroke (NIH/NINDS)
R01NS081248
米国
引用
ジャーナル: Nat Commun / 年: 2017 タイトル: The CaMKII holoenzyme structure in activation-competent conformations. 著者: Janette B Myers / Vincent Zaegel / Steven J Coultrap / Adam P Miller / K Ulrich Bayer / Steve L Reichow / 要旨: The Ca/calmodulin-dependent protein kinase II (CaMKII) assembles into large 12-meric holoenzymes, which is thought to enable regulatory processes required for synaptic plasticity underlying learning, ...The Ca/calmodulin-dependent protein kinase II (CaMKII) assembles into large 12-meric holoenzymes, which is thought to enable regulatory processes required for synaptic plasticity underlying learning, memory and cognition. Here we used single particle electron microscopy (EM) to determine a pseudoatomic model of the CaMKIIα holoenzyme in an extended and activation-competent conformation. The holoenzyme is organized by a rigid central hub complex, while positioning of the kinase domains is highly flexible, revealing dynamic holoenzymes ranging from 15-35 nm in diameter. While most kinase domains are ordered independently, ∼20% appear to form dimers and <3% are consistent with a compact conformation. An additional level of plasticity is revealed by a small fraction of bona-fide 14-mers (<4%) that may enable subunit exchange. Biochemical and cellular FRET studies confirm that the extended state of CaMKIIα resolved by EM is the predominant form of the holoenzyme, even under molecular crowding conditions.
A: Calcium/calmodulin-dependent protein kinase type II subunit alpha B: Calcium/calmodulin-dependent protein kinase type II subunit alpha C: Calcium/calmodulin-dependent protein kinase type II subunit alpha D: Calcium/calmodulin-dependent protein kinase type II subunit alpha E: Calcium/calmodulin-dependent protein kinase type II subunit alpha F: Calcium/calmodulin-dependent protein kinase type II subunit alpha G: Calcium/calmodulin-dependent protein kinase type II subunit alpha H: Calcium/calmodulin-dependent protein kinase type II subunit alpha I: Calcium/calmodulin-dependent protein kinase type II subunit alpha J: Calcium/calmodulin-dependent protein kinase type II subunit alpha K: Calcium/calmodulin-dependent protein kinase type II subunit alpha L: Calcium/calmodulin-dependent protein kinase type II subunit alpha
平均露光時間: 1 sec. / 電子線照射量: 20 e/Å2 / フィルム・検出器のモデル: FEI EAGLE (2k x 2k)
-
解析
EMソフトウェア
ID
名称
バージョン
カテゴリ
詳細
1
EMAN
2.12
粒子像選択
Handpicked
4
EMAN
2.12
CTF補正
7
UCSF Chimera
1.11.2
モデルフィッティング
10
EMAN
2.12
初期オイラー角割当
11
RELION
1.4
最終オイラー角割当
12
RELION
1.4
分類
13
RELION
1.4
3次元再構成
CTF補正
詳細: Performed in EMAN2 / タイプ: PHASE FLIPPING AND AMPLITUDE CORRECTION
粒子像の選択
選択した粒子像数: 16616
対称性
点対称性: D6 (2回x6回 2面回転対称)
3次元再構成
解像度: 20 Å / 解像度の算出法: FSC 0.143 CUT-OFF / 粒子像の数: 2000 詳細: Only modeled the unstructured linker region, residues 300-345. The rest came from two other, previously published structures, namely 5IG3 and 2VZ6 クラス平均像の数: 1 / 対称性のタイプ: POINT
原子モデル構築
プロトコル: RIGID BODY FIT / Target criteria: Correlation Coefficient
原子モデル構築
3D fitting-ID: 1 / Source name: PDB / タイプ: experimental model