ジャーナル: Elife / 年: 2015 タイトル: The near-atomic cryoEM structure of a flexible filamentous plant virus shows homology of its coat protein with nucleoproteins of animal viruses. 著者: Xabier Agirrezabala / Eduardo Méndez-López / Gorka Lasso / M Amelia Sánchez-Pina / Miguel Aranda / Mikel Valle / 要旨: Flexible filamentous viruses include economically important plant pathogens. Their viral particles contain several hundred copies of a helically arrayed coat protein (CP) protecting a (+)ssRNA. We ...Flexible filamentous viruses include economically important plant pathogens. Their viral particles contain several hundred copies of a helically arrayed coat protein (CP) protecting a (+)ssRNA. We describe here a structure at 3.9 Å resolution, from electron cryomicroscopy, of Pepino mosaic virus (PepMV), a representative of the genus Potexvirus (family Alphaflexiviridae). Our results allow modeling of the CP and its interactions with viral RNA. The overall fold of PepMV CP resembles that of nucleoproteins (NPs) from the genus Phlebovirus (family Bunyaviridae), a group of enveloped (-)ssRNA viruses. The main difference between potexvirus CP and phlebovirus NP is in their C-terminal extensions, which appear to determine the characteristics of the distinct multimeric assemblies - a flexuous, helical rod or a loose ribonucleoprotein. The homology suggests gene transfer between eukaryotic (+) and (-)ssRNA viruses.
履歴
登録
2015年11月10日
登録サイト: PDBE / 処理サイト: PDBE
改定 1.0
2015年12月30日
Provider: repository / タイプ: Initial release
改定 1.1
2016年12月21日
Group: Database references
改定 1.2
2017年8月30日
Group: Data collection / カテゴリ: em_software / Item: _em_software.image_processing_id