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- PDB-4uq8: Electron cryo-microscopy of bovine Complex I -

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Basic information

Entry
Database: PDB / ID: 4uq8
TitleElectron cryo-microscopy of bovine Complex I
Components
  • (NADH DEHYDROGENASE [UBIQUINONE] 1 ALPHA SUBCOMPLEX SUBUNIT ...) x 5
  • (NADH DEHYDROGENASE [UBIQUINONE] 1 ALPHA SUBCOMPLEX SUBUNIT; ...) x 3
  • (NADH DEHYDROGENASE [UBIQUINONE] FLAVOPROTEIN ...) x 2
  • (NADH DEHYDROGENASE [UBIQUINONE] IRON-SULFUR PROTEIN ...) x 4
  • (NADH DEHYDROGENASE [UBIQUINONE] SUBUNIT ...) x 2
  • (NADH UBIQUINONE ...) x 25
  • ACYL CARRIER PROTEIN, MITOCHONDRIAL
  • NADH-UBIQUINONE OXIDOREDUCTASE 75 KDA SUBUNIT, MITOCHONDRIAL
KeywordsOXIDOREDUCTASE / NADH DEHYDROGENASE / RESPIRATORY COMPLEX
Function / homologyFE2/S2 (INORGANIC) CLUSTER / IRON/SULFUR CLUSTER
Function and homology information
Biological speciesBOS TAURUS (cattle)
MethodELECTRON MICROSCOPY / single particle reconstruction / cryo EM / Resolution: 4.95 Å
AuthorsVinothkumar, K.R. / Zhu, J. / Hirst, J.
CitationJournal: Nature / Year: 2014
Title: Architecture of mammalian respiratory complex I.
Authors: Kutti R Vinothkumar / Jiapeng Zhu / Judy Hirst /
Abstract: Complex I (NADH:ubiquinone oxidoreductase) is essential for oxidative phosphorylation in mammalian mitochondria. It couples electron transfer from NADH to ubiquinone with proton translocation across ...Complex I (NADH:ubiquinone oxidoreductase) is essential for oxidative phosphorylation in mammalian mitochondria. It couples electron transfer from NADH to ubiquinone with proton translocation across the energy-transducing inner membrane, providing electrons for respiration and driving ATP synthesis. Mammalian complex I contains 44 different nuclear- and mitochondrial-encoded subunits, with a combined mass of 1 MDa. The 14 conserved 'core' subunits have been structurally defined in the minimal, bacterial complex, but the structures and arrangement of the 30 'supernumerary' subunits are unknown. Here we describe a 5 Å resolution structure of complex I from Bos taurus heart mitochondria, a close relative of the human enzyme, determined by single-particle electron cryo-microscopy. We present the structures of the mammalian core subunits that contain eight iron-sulphur clusters and 60 transmembrane helices, identify 18 supernumerary transmembrane helices, and assign and model 14 supernumerary subunits. Thus, we considerably advance knowledge of the structure of mammalian complex I and the architecture of its supernumerary ensemble around the core domains. Our structure provides insights into the roles of the supernumerary subunits in regulation, assembly and homeostasis, and a basis for understanding the effects of mutations that cause a diverse range of human diseases.
History
DepositionJun 21, 2014Deposition site: PDBE / Processing site: PDBE
Revision 1.0Oct 1, 2014Provider: repository / Type: Initial release
Revision 1.1Nov 12, 2014Group: Database references
Revision 1.2Aug 2, 2017Group: Data collection / Category: em_software
Item: _em_software.fitting_id / _em_software.image_processing_id / _em_software.name
Revision 1.3May 8, 2024Group: Data collection / Database references / Category: chem_comp_atom / chem_comp_bond / database_2
Item: _database_2.pdbx_DOI / _database_2.pdbx_database_accession
Remark 700 SHEET DETERMINATION METHOD: AUTHOR PROVIDED.

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Structure visualization

Movie
  • Deposited structure unit
  • Imaged by Jmol
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  • Superimposition on EM map
  • EMDB-2676
  • Imaged by UCSF Chimera
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Structure viewerMolecule:
MolmilJmol/JSmol

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Assembly

Deposited unit
A: NADH UBIQUINONE OXIDOREDUCTASE CHAIN 3
B: NADH DEHYDROGENASE [UBIQUINONE] IRON-SULFUR PROTEIN 7, MITOCHONDRIAL
C: NADH DEHYDROGENASE [UBIQUINONE] IRON-SULFUR PROTEIN 3, MITOCHONDRIAL
D: NADH DEHYDROGENASE [UBIQUINONE] IRON-SULFUR PROTEIN 2, MITOCHONDRIAL
E: NADH DEHYDROGENASE [UBIQUINONE] FLAVOPROTEIN 2, MITOCHONDRIAL
F: NADH DEHYDROGENASE [UBIQUINONE] FLAVOPROTEIN 1, MITOCHONDRIAL
G: NADH-UBIQUINONE OXIDOREDUCTASE 75 KDA SUBUNIT, MITOCHONDRIAL
H: NADH UBIQUINONE OXIDOREDUCTASE CHAIN 1
I: NADH DEHYDROGENASE [UBIQUINONE] IRON-SULFUR PROTEIN 8, MITOCHONDRIAL
J: NADH UBIQUINONE OXIDOREDUCTASE CHAIN 6
K: NADH UBIQUINONE OXIDOREDUCTASE CHAIN 4L
L: NADH UBIQUINONE OXIDOREDUCTASE CHAIN 5
M: NADH UBIQUINONE OXIDOREDUCTASE CHAIN 4
N: NADH UBIQUINONE OXIDOREDUCTASE CHAIN 2
O: NADH DEHYDROGENASE [UBIQUINONE] 1 ALPHA SUBCOMPLEX SUBUNIT 10
P: NADH DEHYDROGENASE [UBIQUINONE] 1 ALPHA SUBCOMPLEX SUBUNIT 9, MITOCHONDRIAL
Q: NADH DEHYDROGENASE [UBIQUINONE] SUBUNIT 4
R: NADH DEHYDROGENASE [UBIQUINONE] SUBUNIT 6
S: NADH DEHYDROGENASE [UBIQUINONE] 1 ALPHA SUBCOMPLEX SUBUNIT 2
T: ACYL CARRIER PROTEIN, MITOCHONDRIAL
U: NADH UBIQUINONE DEHYDROGENASE
V: NADH DEHYDROGENASE [UBIQUINONE] 1 ALPHA SUBCOMPLEX SUBUNIT; 5
W: NADH DEHYDROGENASE [UBIQUINONE] 1 ALPHA SUBCOMPLEX SUBUNIT; 6
X: NADH DEHYDROGENASE [UBIQUINONE] 1 ALPHA SUBCOMPLEX SUBUNIT 8
Y: NADH DEHYDROGENASE [UBIQUINONE] 1 ALPHA SUBCOMPLEX SUBUNIT; 11
Z: NADH DEHYDROGENASE [UBIQUINONE] 1 ALPHA SUBCOMPLEX SUBUNIT 13
a: NADH UBIQUINONE OXIDOREDUCTASE
b: NADH UBIQUINONE OXIDOREDUCTASE
c: NADH UBIQUINONE OXIDOREDUCTASE
d: NADH UBIQUINONE OXIDOREDUCTASE
e: NADH UBIQUINONE OXIDOREDUCTASE
f: NADH UBIQUINONE OXIDOREDUCTASE
g: NADH UBIQUINONE OXIDOREDUCTASE
h: NADH UBIQUINONE OXIDOREDUCTASE
i: NADH UBIQUINONE OXIDOREDUCTASE
j: NADH UBIQUINONE OXIDOREDUCTASE
k: NADH UBIQUINONE OXIDOREDUCTASE
l: NADH UBIQUINONE OXIDOREDUCTASE
m: NADH UBIQUINONE OXIDOREDUCTASE
n: NADH UBIQUINONE OXIDOREDUCTASE
o: NADH UBIQUINONE OXIDOREDUCTASE
p: NADH UBIQUINONE OXIDOREDUCTASE
q: NADH UBIQUINONE OXIDOREDUCTASE
r: NADH UBIQUINONE OXIDOREDUCTASE
s: NADH UBIQUINONE OXIDOREDUCTASE
t: NADH UBIQUINONE OXIDOREDUCTASE
u: NADH UBIQUINONE OXIDOREDUCTASE
v: NADH UBIQUINONE OXIDOREDUCTASE
w: NADH UBIQUINONE OXIDOREDUCTASE
hetero molecules


Theoretical massNumber of molelcules
Total (without water)517,63557
Polymers515,17449
Non-polymers2,4618
Water00
1


  • Idetical with deposited unit
  • defined by software
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
MethodPQS

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Components

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NADH UBIQUINONE ... , 25 types, 31 molecules AHJKLMNUabcwdefhigjlkpsmnoqrtuv

#1: Protein NADH UBIQUINONE OXIDOREDUCTASE CHAIN 3


Mass: 9464.658 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) BOS TAURUS (cattle) / Organ: HEART
#8: Protein NADH UBIQUINONE OXIDOREDUCTASE CHAIN 1


Mass: 26655.701 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) BOS TAURUS (cattle) / Organ: HEART
#10: Protein NADH UBIQUINONE OXIDOREDUCTASE CHAIN 6


Mass: 14570.941 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) BOS TAURUS (cattle) / Organ: HEART
#11: Protein NADH UBIQUINONE OXIDOREDUCTASE CHAIN 4L


Mass: 7166.825 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) BOS TAURUS (cattle) / Organ: HEART
#12: Protein NADH UBIQUINONE OXIDOREDUCTASE CHAIN 5


Mass: 51166.188 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) BOS TAURUS (cattle) / Organ: HEART
#13: Protein NADH UBIQUINONE OXIDOREDUCTASE CHAIN 4


Mass: 38570.516 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) BOS TAURUS (cattle) / Organ: HEART
#14: Protein NADH UBIQUINONE OXIDOREDUCTASE CHAIN 2


Mass: 29379.080 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) BOS TAURUS (cattle) / Organ: HEART
#21: Protein NADH UBIQUINONE DEHYDROGENASE


Mass: 6727.274 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) BOS TAURUS (cattle) / Organ: HEART
#27: Protein/peptide NADH UBIQUINONE OXIDOREDUCTASE


Mass: 2486.056 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) BOS TAURUS (cattle) / Organ: HEART
#28: Protein/peptide NADH UBIQUINONE OXIDOREDUCTASE


Mass: 3592.419 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) BOS TAURUS (cattle) / Organ: HEART
#29: Protein/peptide NADH UBIQUINONE OXIDOREDUCTASE


Mass: 2315.846 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) BOS TAURUS (cattle) / Organ: HEART
#30: Protein/peptide NADH UBIQUINONE OXIDOREDUCTASE


Mass: 3337.105 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) BOS TAURUS (cattle) / Organ: HEART
#31: Protein/peptide NADH UBIQUINONE OXIDOREDUCTASE


Mass: 1720.111 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) BOS TAURUS (cattle) / Organ: HEART
#32: Protein/peptide NADH UBIQUINONE OXIDOREDUCTASE


Mass: 2571.161 Da / Num. of mol.: 3 / Source method: isolated from a natural source / Source: (natural) BOS TAURUS (cattle) / Organ: HEART
#33: Protein/peptide NADH UBIQUINONE OXIDOREDUCTASE


Mass: 1890.321 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) BOS TAURUS (cattle) / Organ: HEART
#34: Protein/peptide NADH UBIQUINONE OXIDOREDUCTASE


Mass: 2060.531 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) BOS TAURUS (cattle) / Organ: HEART
#35: Protein/peptide NADH UBIQUINONE OXIDOREDUCTASE


Mass: 2400.951 Da / Num. of mol.: 3 / Source method: isolated from a natural source / Source: (natural) BOS TAURUS (cattle) / Organ: HEART
#36: Protein/peptide NADH UBIQUINONE OXIDOREDUCTASE


Mass: 2911.580 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) BOS TAURUS (cattle) / Organ: HEART
#37: Protein NADH UBIQUINONE OXIDOREDUCTASE


Mass: 5039.203 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) BOS TAURUS (cattle) / Organ: HEART
#38: Protein/peptide NADH UBIQUINONE OXIDOREDUCTASE


Mass: 1805.216 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) BOS TAURUS (cattle) / Organ: HEART
#39: Protein/peptide NADH UBIQUINONE OXIDOREDUCTASE


Mass: 2145.636 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) BOS TAURUS (cattle) / Organ: HEART
#40: Protein/peptide NADH UBIQUINONE OXIDOREDUCTASE


Mass: 2230.741 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) BOS TAURUS (cattle) / Organ: HEART
#41: Protein NADH UBIQUINONE OXIDOREDUCTASE


Mass: 4868.993 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) BOS TAURUS (cattle) / Organ: HEART
#42: Protein/peptide NADH UBIQUINONE OXIDOREDUCTASE


Mass: 3677.524 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) BOS TAURUS (cattle) / Organ: HEART
#43: Protein/peptide NADH UBIQUINONE OXIDOREDUCTASE


Mass: 2741.370 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) BOS TAURUS (cattle) / Organ: HEART

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NADH DEHYDROGENASE [UBIQUINONE] IRON-SULFUR PROTEIN ... , 4 types, 4 molecules BCDI

#2: Protein NADH DEHYDROGENASE [UBIQUINONE] IRON-SULFUR PROTEIN 7, MITOCHONDRIAL


Mass: 12260.168 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) BOS TAURUS (cattle) / Organ: HEART
#3: Protein NADH DEHYDROGENASE [UBIQUINONE] IRON-SULFUR PROTEIN 3, MITOCHONDRIAL


Mass: 13124.170 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) BOS TAURUS (cattle) / Organ: HEART
#4: Protein NADH DEHYDROGENASE [UBIQUINONE] IRON-SULFUR PROTEIN 2, MITOCHONDRIAL


Mass: 32698.209 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) BOS TAURUS (cattle) / Organ: HEART
#9: Protein NADH DEHYDROGENASE [UBIQUINONE] IRON-SULFUR PROTEIN 8, MITOCHONDRIAL


Mass: 13949.309 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) BOS TAURUS (cattle) / Organ: HEART

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NADH DEHYDROGENASE [UBIQUINONE] FLAVOPROTEIN ... , 2 types, 2 molecules EF

#5: Protein NADH DEHYDROGENASE [UBIQUINONE] FLAVOPROTEIN 2, MITOCHONDRIAL


Mass: 13621.847 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) BOS TAURUS (cattle) / Organ: HEART
#6: Protein NADH DEHYDROGENASE [UBIQUINONE] FLAVOPROTEIN 1, MITOCHONDRIAL


Mass: 35068.211 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) BOS TAURUS (cattle) / Organ: HEART

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Protein , 2 types, 2 molecules GT

#7: Protein NADH-UBIQUINONE OXIDOREDUCTASE 75 KDA SUBUNIT, MITOCHONDRIAL


Mass: 46055.980 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) BOS TAURUS (cattle) / Organ: HEART
#20: Protein ACYL CARRIER PROTEIN, MITOCHONDRIAL


Mass: 6400.881 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) BOS TAURUS (cattle) / Organ: HEART

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NADH DEHYDROGENASE [UBIQUINONE] 1 ALPHA SUBCOMPLEX SUBUNIT ... , 5 types, 5 molecules OPSXZ

#15: Protein NADH DEHYDROGENASE [UBIQUINONE] 1 ALPHA SUBCOMPLEX SUBUNIT 10


Mass: 18741.035 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) BOS TAURUS (cattle) / Organ: HEART
#16: Protein NADH DEHYDROGENASE [UBIQUINONE] 1 ALPHA SUBCOMPLEX SUBUNIT 9, MITOCHONDRIAL


Mass: 25804.662 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) BOS TAURUS (cattle) / Organ: HEART
#19: Protein NADH DEHYDROGENASE [UBIQUINONE] 1 ALPHA SUBCOMPLEX SUBUNIT 2


Mass: 6826.406 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) BOS TAURUS (cattle) / Organ: HEART
#24: Protein NADH DEHYDROGENASE [UBIQUINONE] 1 ALPHA SUBCOMPLEX SUBUNIT 8


Mass: 6741.301 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) BOS TAURUS (cattle) / Organ: HEART
#26: Protein NADH DEHYDROGENASE [UBIQUINONE] 1 ALPHA SUBCOMPLEX SUBUNIT 13


Mass: 5549.833 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) BOS TAURUS (cattle) / Organ: HEART

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NADH DEHYDROGENASE [UBIQUINONE] SUBUNIT ... , 2 types, 2 molecules QR

#17: Protein NADH DEHYDROGENASE [UBIQUINONE] SUBUNIT 4


Mass: 7251.930 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) BOS TAURUS (cattle) / Organ: HEART
#18: Protein/peptide NADH DEHYDROGENASE [UBIQUINONE] SUBUNIT 6


Mass: 4017.944 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) BOS TAURUS (cattle) / Organ: HEART

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NADH DEHYDROGENASE [UBIQUINONE] 1 ALPHA SUBCOMPLEX SUBUNIT; ... , 3 types, 3 molecules VWY

#22: Protein NADH DEHYDROGENASE [UBIQUINONE] 1 ALPHA SUBCOMPLEX SUBUNIT; 5


Mass: 6060.462 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) BOS TAURUS (cattle) / Organ: HEART
#23: Protein NADH DEHYDROGENASE [UBIQUINONE] 1 ALPHA SUBCOMPLEX SUBUNIT; 6


Mass: 6145.567 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) BOS TAURUS (cattle) / Organ: HEART
#25: Protein NADH DEHYDROGENASE [UBIQUINONE] 1 ALPHA SUBCOMPLEX SUBUNIT; 11


Mass: 9039.134 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) BOS TAURUS (cattle) / Organ: HEART

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Non-polymers , 2 types, 8 molecules

#44: Chemical
ChemComp-SF4 / IRON/SULFUR CLUSTER


Mass: 351.640 Da / Num. of mol.: 6 / Source method: obtained synthetically / Formula: Fe4S4
#45: Chemical ChemComp-FES / FE2/S2 (INORGANIC) CLUSTER


Mass: 175.820 Da / Num. of mol.: 2 / Source method: obtained synthetically / Formula: Fe2S2

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Experimental details

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Experiment

ExperimentMethod: ELECTRON MICROSCOPY
EM experimentAggregation state: PARTICLE / 3D reconstruction method: single particle reconstruction

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Sample preparation

ComponentName: NADH-UBIQUINONE OXIDOREDUCTASE / Type: COMPLEX / Details: ONE IMAGE PER HOLE WAS TAKEN.
Buffer solutionName: 20MM TRIS-HCL, 150 MM NACL, 0.03% CYMAL-7 / pH: 8 / Details: 20MM TRIS-HCL, 150 MM NACL, 0.03% CYMAL-7
SpecimenConc.: 3.5 mg/ml / Embedding applied: NO / Shadowing applied: NO / Staining applied: NO / Vitrification applied: YES
Specimen supportDetails: HOLEY CARBON
VitrificationCryogen name: ETHANE
Details: VITRIFICATION 1 -- CRYOGEN- ETHANE, HUMIDITY- 100, TEMPERATURE- 100, INSTRUMENT- OTHER, METHOD- THE SPECIMEN WAS VITRIFIED IN AN ENVIRONMENTAL PLUNGE-FREEZE APPARATUS (BELLARE ET AL, J. ...Details: VITRIFICATION 1 -- CRYOGEN- ETHANE, HUMIDITY- 100, TEMPERATURE- 100, INSTRUMENT- OTHER, METHOD- THE SPECIMEN WAS VITRIFIED IN AN ENVIRONMENTAL PLUNGE-FREEZE APPARATUS (BELLARE ET AL, J.ELECTR. MICROS. TECH., 1988, 10, 87-111). BLOT FOR 15- 18 SECONDS AFTER THE DIAMETER OF THE BLOTTED MENISCUS CEASES TO EXPAND AND PLUNGED.,

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Electron microscopy imaging

Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company
MicroscopyModel: FEI TITAN KRIOS / Date: Oct 21, 2013
Details: EACH IMAGE WAS EXPOSED FOR 4 SECONDS RESULTING IN A TOTAL DOSE OF ABOUT 64 ELECTRONS.
Electron gunElectron source: FIELD EMISSION GUN / Accelerating voltage: 300 kV / Illumination mode: FLOOD BEAM
Electron lensMode: BRIGHT FIELD / Nominal magnification: 47000 X / Calibrated magnification: 81495 X / Nominal defocus max: 5000 nm / Nominal defocus min: 2000 nm / Cs: 2.7 mm
Specimen holderTemperature: 85 K
Image recordingElectron dose: 64 e/Å2 / Film or detector model: FEI FALCON II (4k x 4k)
Image scansNum. digital images: 1154
Radiation wavelengthRelative weight: 1

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Processing

EM software
IDNameCategory
1UCSF Chimeramodel fitting
2RELION3D reconstruction
CTF correctionDetails: EACH PARTICLE
SymmetryPoint symmetry: C1 (asymmetric)
3D reconstructionMethod: RELION / Resolution: 4.95 Å / Num. of particles: 25492 / Actual pixel size: 1.717 Å
Details: SUBMISSION BASED ON EXPERIMENTAL DATA FROM EMDB EMD -2676(DEPOSITION ID: 12566).AUTHORS HAVE USED COOT TO FIX SOME CLOSE CONTACTS AND RESET THE B-FACTOR. AS THIS MODEL IS NOT REFINED, HENCE ...Details: SUBMISSION BASED ON EXPERIMENTAL DATA FROM EMDB EMD -2676(DEPOSITION ID: 12566).AUTHORS HAVE USED COOT TO FIX SOME CLOSE CONTACTS AND RESET THE B-FACTOR. AS THIS MODEL IS NOT REFINED, HENCE THE B-FACTOR DOES NOT MEAN ANYTHING.
Symmetry type: POINT
Atomic model buildingSpace: REAL
RefinementHighest resolution: 4.95 Å
Refinement stepCycle: LAST / Highest resolution: 4.95 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms28591 0 56 0 28647

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