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- PDB-3jcu: Cryo-EM structure of spinach PSII-LHCII supercomplex at 3.2 Angst... -
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Basic information
Entry | Database: PDB / ID: 3jcu | ||||||
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Title | Cryo-EM structure of spinach PSII-LHCII supercomplex at 3.2 Angstrom resolution | ||||||
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![]() | MEMBRANE PROTEIN | ||||||
Function / homology | ![]() chloroplast photosystem II / photosynthesis, light harvesting / plastoglobule / : / photosystem II assembly / photosynthesis, light harvesting in photosystem I / photosystem II oxygen evolving complex / oxygen evolving activity / photosystem II stabilization / photosystem II ...chloroplast photosystem II / photosynthesis, light harvesting / plastoglobule / : / photosystem II assembly / photosynthesis, light harvesting in photosystem I / photosystem II oxygen evolving complex / oxygen evolving activity / photosystem II stabilization / photosystem II / photosystem II reaction center / : / ion binding / chloroplast envelope / oxidoreductase activity, acting on diphenols and related substances as donors, oxygen as acceptor / photosystem I / photosynthetic electron transport chain / response to herbicide / photosystem II / extrinsic component of membrane / chlorophyll binding / photosynthesis, light reaction / electron transporter, transferring electrons within the cyclic electron transport pathway of photosynthesis activity / phosphate ion binding / chloroplast thylakoid membrane / response to light stimulus / photosynthetic electron transport in photosystem II / photosynthesis / phosphoprotein binding / membrane => GO:0016020 / electron transfer activity / protein stabilization / iron ion binding / calcium ion binding / heme binding / metal ion binding Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ELECTRON MICROSCOPY / single particle reconstruction / cryo EM / Resolution: 3.2 Å | ||||||
![]() | Wei, X.P. / Zhang, X.Z. / Su, X.D. / Cao, P. / Liu, X.Y. / Li, M. / Chang, W.R. / Liu, Z.F. | ||||||
![]() | ![]() Title: Structure of spinach photosystem II-LHCII supercomplex at 3.2 Å resolution. Authors: Xuepeng Wei / Xiaodong Su / Peng Cao / Xiuying Liu / Wenrui Chang / Mei Li / Xinzheng Zhang / Zhenfeng Liu / ![]() Abstract: During photosynthesis, the plant photosystem II core complex receives excitation energy from the peripheral light-harvesting complex II (LHCII). The pathways along which excitation energy is ...During photosynthesis, the plant photosystem II core complex receives excitation energy from the peripheral light-harvesting complex II (LHCII). The pathways along which excitation energy is transferred between them, and their assembly mechanisms, remain to be deciphered through high-resolution structural studies. Here we report the structure of a 1.1-megadalton spinach photosystem II-LHCII supercomplex solved at 3.2 Å resolution through single-particle cryo-electron microscopy. The structure reveals a homodimeric supramolecular system in which each monomer contains 25 protein subunits, 105 chlorophylls, 28 carotenoids and other cofactors. Three extrinsic subunits (PsbO, PsbP and PsbQ), which are essential for optimal oxygen-evolving activity of photosystem II, form a triangular crown that shields the Mn4CaO5-binding domains of CP43 and D1. One major trimeric and two minor monomeric LHCIIs associate with each core-complex monomer, and the antenna-core interactions are reinforced by three small intrinsic subunits (PsbW, PsbH and PsbZ). By analysing the closely connected interfacial chlorophylls, we have obtained detailed insights into the energy-transfer pathways between the antenna and core complexes. | ||||||
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PDBx/mmCIF format | ![]() | 1.9 MB | Display | ![]() |
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PDB format | ![]() | 1.7 MB | Display | ![]() |
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-Validation report
Summary document | ![]() | 13.8 MB | Display | ![]() |
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Full document | ![]() | 14.7 MB | Display | |
Data in XML | ![]() | 330.5 KB | Display | |
Data in CIF | ![]() | 419.6 KB | Display | |
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-Related structure data
Related structure data | ![]() 6617MC M: map data used to model this data C: citing same article ( |
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Similar structure data |
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Assembly
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Components
-Photosystem II ... , 13 types, 26 molecules AaBbCcDdHhJjKkMmTtUuWwXxZz
#1: Protein | Mass: 38134.430 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) ![]() #2: Protein | Mass: 56207.852 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) ![]() #3: Protein | Mass: 51880.559 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) ![]() #4: Protein | Mass: 39535.172 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) ![]() #8: Protein | Mass: 7735.004 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) ![]() #10: Protein/peptide | Mass: 4117.832 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) ![]() #11: Protein | Mass: 6751.023 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) ![]() #13: Protein/peptide | Mass: 3783.538 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) ![]() #19: Protein/peptide | Mass: 3825.642 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) ![]() #20: Protein | Mass: 10676.572 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) ![]() #21: Protein | Mass: 14186.097 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) ![]() #22: Protein | Mass: 11958.141 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) ![]() #23: Protein | Mass: 6541.715 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) ![]() |
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-Cytochrome b559 subunit ... , 2 types, 4 molecules EeFf
#5: Protein | Mass: 9393.501 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) ![]() #6: Protein/peptide | Mass: 4502.351 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) ![]() |
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-Protein Photosystem II reaction center protein ... , 2 types, 4 molecules IiLl
#9: Protein/peptide | Mass: 4170.891 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) ![]() #12: Protein/peptide | Mass: 4498.100 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) ![]() |
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-Oxygen-evolving enhancer protein ... , 3 types, 6 molecules OoPpQq
#14: Protein | Mass: 35207.383 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) ![]() #15: Protein | Mass: 28495.918 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) ![]() #16: Protein | Mass: 24884.426 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) ![]() |
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-Chlorophyll A-B Binding protein ... , 2 types, 4 molecules RrSs
#17: Protein | Mass: 26735.303 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) ![]() #18: Protein | Mass: 31341.840 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) ![]() |
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-Protein / Sugars , 2 types, 14 molecules GNYgny![](data/chem/img/DGD.gif)
![](data/chem/img/DGD.gif)
#32: Sugar | ChemComp-DGD / #7: Protein | Mass: 28449.391 Da / Num. of mol.: 6 / Source method: isolated from a natural source / Source: (natural) ![]() |
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-Non-polymers , 16 types, 314 molecules ![](data/chem/img/OEX.gif)
![](data/chem/img/FE2.gif)
![](data/chem/img/CL.gif)
![](data/chem/img/CLA.gif)
![](data/chem/img/PHO.gif)
![](data/chem/img/BCR.gif)
![](data/chem/img/SQD.gif)
![](data/chem/img/LMG.gif)
![](data/chem/img/BCT.gif)
![](data/chem/img/PL9.gif)
![](data/chem/img/LHG.gif)
![](data/chem/img/HEM.gif)
![](data/chem/img/CHL.gif)
![](data/chem/img/LUT.gif)
![](data/chem/img/XAT.gif)
![](data/chem/img/NEX.gif)
![](data/chem/img/FE2.gif)
![](data/chem/img/CL.gif)
![](data/chem/img/CLA.gif)
![](data/chem/img/PHO.gif)
![](data/chem/img/BCR.gif)
![](data/chem/img/SQD.gif)
![](data/chem/img/LMG.gif)
![](data/chem/img/BCT.gif)
![](data/chem/img/PL9.gif)
![](data/chem/img/LHG.gif)
![](data/chem/img/HEM.gif)
![](data/chem/img/CHL.gif)
![](data/chem/img/LUT.gif)
![](data/chem/img/XAT.gif)
![](data/chem/img/NEX.gif)
#24: Chemical | #25: Chemical | #26: Chemical | ChemComp-CL / #27: Chemical | ChemComp-CLA / #28: Chemical | ChemComp-PHO / #29: Chemical | ChemComp-BCR / #30: Chemical | ChemComp-SQD / #31: Chemical | ChemComp-LMG / #33: Chemical | #34: Chemical | #35: Chemical | ChemComp-LHG / #36: Chemical | #37: Chemical | ChemComp-CHL / #38: Chemical | ChemComp-LUT / ( #39: Chemical | ChemComp-XAT / ( #40: Chemical | ChemComp-NEX / ( |
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