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- PDB-11dg: Chimeric Escherichia coli 70S ribosome containing an evolved Vibr... -

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基本情報

登録情報
データベース: PDB / ID: 11dg
タイトルChimeric Escherichia coli 70S ribosome containing an evolved Vibrio cholerae 16S rRNA (VC-S4.4)
要素
  • (30S ribosomal protein ...) x 2
  • (50S ribosomal protein ...) x 12
  • (Large ribosomal subunit protein ...) x 17
  • (Small ribosomal subunit protein ...) x 18
  • 16S ribosomal RNA
  • 23S ribosomal RNA
  • 5S ribosomal RNA
  • E-site tRNA
キーワードRIBOSOME / Directed evolution / translation
機能・相同性
機能・相同性情報


negative regulation of cytoplasmic translational initiation / misfolded RNA binding / Group I intron splicing / RNA folding / transcriptional attenuation / endoribonuclease inhibitor activity / positive regulation of ribosome biogenesis / RNA-binding transcription regulator activity / four-way junction DNA binding / negative regulation of cytoplasmic translation ...negative regulation of cytoplasmic translational initiation / misfolded RNA binding / Group I intron splicing / RNA folding / transcriptional attenuation / endoribonuclease inhibitor activity / positive regulation of ribosome biogenesis / RNA-binding transcription regulator activity / four-way junction DNA binding / negative regulation of cytoplasmic translation / DnaA-L2 complex / translation repressor activity / negative regulation of translational initiation / negative regulation of DNA-templated DNA replication initiation / mRNA regulatory element binding translation repressor activity / positive regulation of RNA splicing / cytosolic ribosome assembly / ribosome assembly / assembly of large subunit precursor of preribosome / transcription antitermination / DNA endonuclease activity / translational initiation / regulation of cell growth / response to radiation / DNA-templated transcription termination / maintenance of translational fidelity / mRNA 5'-UTR binding / large ribosomal subunit / ribosomal small subunit assembly / transferase activity / ribosome binding / ribosomal small subunit biogenesis / 5S rRNA binding / small ribosomal subunit / ribosomal large subunit assembly / small ribosomal subunit rRNA binding / cytosolic small ribosomal subunit / large ribosomal subunit rRNA binding / cytosolic large ribosomal subunit / cytoplasmic translation / tRNA binding / negative regulation of translation / rRNA binding / structural constituent of ribosome / ribosome / translation / ribonucleoprotein complex / response to antibiotic / negative regulation of DNA-templated transcription / hydrolase activity / mRNA binding / DNA binding / RNA binding / zinc ion binding / membrane / cytoplasm / cytosol
類似検索 - 分子機能
Ribosomal protein S21, conserved site / Ribosomal protein S21 signature. / Ribosomal protein L25, short-form / Ribosomal protein S14, bacterial/plastid / Ribosomal protein L31 type A / Ribosomal protein S21 superfamily / Ribosomal protein S16, conserved site / Ribosomal protein S16 signature. / Ribosomal protein S21 / Ribosomal protein L31 signature. ...Ribosomal protein S21, conserved site / Ribosomal protein S21 signature. / Ribosomal protein L25, short-form / Ribosomal protein S14, bacterial/plastid / Ribosomal protein L31 type A / Ribosomal protein S21 superfamily / Ribosomal protein S16, conserved site / Ribosomal protein S16 signature. / Ribosomal protein S21 / Ribosomal protein L31 signature. / Ribosomal protein L31 / Ribosomal protein L31 superfamily / Ribosomal protein L31 / Ribosomal protein S21 / Ribosomal protein L9 signature. / Ribosomal protein L16 signature 1. / Ribosomal protein L6, conserved site / Ribosomal protein L6 signature 1. / Ribosomal protein L21, conserved site / Ribosomal protein L21 signature. / Ribosomal protein L9, bacteria/chloroplast / Ribosomal protein L9, C-terminal / Ribosomal protein L9, C-terminal domain / : / Ribosomal protein L9, C-terminal domain superfamily / Ribosomal protein L16 signature 2. / Ribosomal protein L16, conserved site / Ribosomal protein L17 signature. / Ribosomal L25p family / Ribosomal protein L25 / Ribosomal protein L36 signature. / Ribosomal protein L25/Gln-tRNA synthetase, N-terminal / Ribosomal protein L25/Gln-tRNA synthetase, anti-codon-binding domain superfamily / : / Ribosomal protein L28/L24 superfamily / Ribosomal protein L33, conserved site / Ribosomal protein L33 signature. / Ribosomal protein L32p, bacterial type / Ribosomal protein L35, conserved site / Ribosomal protein L35 signature. / Ribosomal protein L9 / Ribosomal protein L9, N-terminal domain superfamily / Ribosomal protein L9, N-terminal / Ribosomal protein L9, N-terminal domain / Ribosomal protein L28 / Ribosomal protein L35, non-mitochondrial / Ribosomal protein L18, bacterial-type / Ribosomal protein S6, conserved site / Ribosomal protein S6 signature. / Ribosomal protein S3, bacterial-type / Ribosomal protein S13, bacterial-type / : / Ribosomal protein S19, bacterial-type / Ribosomal protein L6, bacterial-type / Ribosomal protein S7, bacterial/organellar-type / Ribosomal protein S11, bacterial-type / Ribosomal protein S20 / Ribosomal protein L5, bacterial-type / Ribosomal protein S20 superfamily / Ribosomal protein S20 / Ribosomal protein S4, bacterial-type / Ribosomal protein L19, conserved site / Ribosomal protein L19 signature. / Ribosomal protein S5, bacterial-type / Ribosomal protein L9/RNase H1, N-terminal / 30S ribosomal protein S17 / : / Ribosomal protein S6, plastid/chloroplast / Ribosomal protein L36 / Ribosomal protein L36 superfamily / Ribosomal protein L36 / Ribosomal protein L20 signature. / Ribosomal protein L34, conserved site / Ribosomal protein L34 signature. / Ribosomal protein L14P, bacterial-type / Ribosomal protein L27, conserved site / Ribosomal protein L27 signature. / Ribosomal protein S2, bacteria/mitochondria/plastid / Ribosomal protein L35 / Ribosomal protein L35 superfamily / Ribosomal protein L22, bacterial/chloroplast-type / Ribosomal protein L35 / Ribosomal protein L2, bacterial/organellar-type / Ribosomal protein L33 / Ribosomal protein L18 / Ribosomal L18 of archaea, bacteria, mitoch. and chloroplast / Ribosomal protein L33 / Ribosomal L28 family / Ribosomal protein S18, conserved site / Ribosomal protein S18 signature. / Ribosomal protein S9, bacterial/plastid / Ribosomal protein L33 superfamily / Ribosomal protein L28/L24 / Ribosomal protein L30, bacterial-type / Ribosomal protein S16 / Ribosomal protein S16 domain superfamily / Ribosomal protein S16 / L28p-like / Ribosomal protein L16 / Ribosomal protein S15, bacterial-type
類似検索 - ドメイン・相同性
SPERMIDINE / SPERMINE / : / RNA / RNA (> 10) / RNA (> 100) / RNA (> 1000) / Small ribosomal subunit protein bS18 / Small ribosomal subunit protein bS21 / Large ribosomal subunit protein bL28 ...SPERMIDINE / SPERMINE / : / RNA / RNA (> 10) / RNA (> 100) / RNA (> 1000) / Small ribosomal subunit protein bS18 / Small ribosomal subunit protein bS21 / Large ribosomal subunit protein bL28 / Small ribosomal subunit protein uS14 / Small ribosomal subunit protein uS13 / Small ribosomal subunit protein bS20 / Small ribosomal subunit protein uS11 / Large ribosomal subunit protein uL16 / Large ribosomal subunit protein bL20 / Large ribosomal subunit protein bL33 / Small ribosomal subunit protein uS10 / Large ribosomal subunit protein uL3 / Large ribosomal subunit protein uL23 / Small ribosomal subunit protein uS19 / Large ribosomal subunit protein uL29 / Small ribosomal subunit protein uS17 / Large ribosomal subunit protein uL24 / Small ribosomal subunit protein uS8 / Large ribosomal subunit protein uL30 / Small ribosomal subunit protein uS9 / Small ribosomal subunit protein uS15 / Large ribosomal subunit protein bL32 / Small ribosomal subunit protein bS6 / Small ribosomal subunit protein uS7 / Large ribosomal subunit protein uL15 / Large ribosomal subunit protein bL19 / Large ribosomal subunit protein bL27 / Large ribosomal subunit protein bL31 / Large ribosomal subunit protein bL34 / Large ribosomal subunit protein bL35 / Large ribosomal subunit protein bL36A / Large ribosomal subunit protein bL9 / Small ribosomal subunit protein uS12 / Small ribosomal subunit protein bS16 / Small ribosomal subunit protein uS2 / Small ribosomal subunit protein uS3 / Small ribosomal subunit protein uS4 / Small ribosomal subunit protein uS5 / Large ribosomal subunit protein uL13 / Large ribosomal subunit protein uL14 / Large ribosomal subunit protein bL17 / Large ribosomal subunit protein bL21 / Large ribosomal subunit protein uL6 / Large ribosomal subunit protein uL18 / Large ribosomal subunit protein uL2 / Large ribosomal subunit protein uL4 / Large ribosomal subunit protein uL22 / Large ribosomal subunit protein uL5 / Large ribosomal subunit protein bL25
類似検索 - 構成要素
生物種Escherichia coli (大腸菌)
手法電子顕微鏡法 / 単粒子再構成法 / クライオ電子顕微鏡法 / 解像度: 3 Å
データ登録者Raskar, T. / Badran, A. / Fraser, J.
資金援助 米国, 2件
組織認可番号国
National Institutes of Health/National Institute Of Allergy and Infectious Diseases (NIH/NIAID)GM145238 米国
National Institutes of Health/Office of the DirectorDP5OD024590 米国
引用ジャーナル: bioRxiv / 年: 2026
タイトル: Structural adaptations for enhanced translation kinetics in evolved ribosomes.
著者: Tushar Raskar / Alan Costello / Ahmed H Badran / James S Fraser /
要旨: The ribosomal RNA sequence governs translation dynamics, yet understanding how changes beyond the conserved catalytic centers influence kinetics and protein yield remains limited. Using orthogonal ...The ribosomal RNA sequence governs translation dynamics, yet understanding how changes beyond the conserved catalytic centers influence kinetics and protein yield remains limited. Using orthogonal ribosome phage-assisted continuous evolution (oRibo-PACE), we recently reported chimeric ribosomes derived from , , and endowed with elevated translation rates as compared to their starting counterparts. Here, we structurally characterize these kinetically enhanced ribosomes using cryo-electron microscopy and uncover a potential relationship between 16S rRNA stability and translation efficiency. Compared to their naive starting points, evolved ribosomes exhibit extensive RNA structural adaptation, often introduced by mismatches at key helical junctions, which leads to local RNA-protein rearrangements and destabilizes non-canonical base pairs. Compensatory mutations that restore base-pairing stability and eliminate flexibility reduced translational activity to wild-type levels. Across trajectories, increased translational output correlates with subtle, localized changes in the 16S rRNA sequence that introduce limited structural destabilization at specific elements. Taken together, our work provides new insights into rRNA structural malleability and establishes principles for engineering ribosomes with altered translation properties.
履歴
登録2026年2月18日登録サイト: RCSB / 処理サイト: RCSB
改定 1.02026年4月8日Provider: repository / タイプ: Initial release
改定 1.02026年4月8日Data content type: EM metadata / Data content type: EM metadata / Provider: repository / タイプ: Initial release
改定 1.02026年4月8日Data content type: FSC / Data content type: FSC / Provider: repository / タイプ: Initial release
改定 1.02026年4月8日Data content type: Half map / Part number: 1 / Data content type: Half map / Provider: repository / タイプ: Initial release
改定 1.02026年4月8日Data content type: Half map / Part number: 2 / Data content type: Half map / Provider: repository / タイプ: Initial release
改定 1.02026年4月8日Data content type: Image / Data content type: Image / Provider: repository / タイプ: Initial release
改定 1.02026年4月8日Data content type: Primary map / Data content type: Primary map / Provider: repository / タイプ: Initial release

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構造の表示

構造ビューア分子:
MolmilJmol/JSmol

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集合体

登録構造単位
0: 50S ribosomal protein L33
1: Large ribosomal subunit protein bL34
2: 50S ribosomal protein L35
3: Large ribosomal subunit protein bL36A
4: 50S ribosomal protein L31
5: E-site tRNA
A: 16S ribosomal RNA
B: Small ribosomal subunit protein uS2
C: 30S ribosomal protein S3
D: Small ribosomal subunit protein uS4
E: Small ribosomal subunit protein uS5
F: Small ribosomal subunit protein bS6
G: Small ribosomal subunit protein uS7
H: Small ribosomal subunit protein uS8
I: Small ribosomal subunit protein uS9
J: Small ribosomal subunit protein uS10
K: Small ribosomal subunit protein uS11
L: 30S ribosomal protein S12
M: Small ribosomal subunit protein uS13
N: Small ribosomal subunit protein uS14
O: Small ribosomal subunit protein uS15
P: Small ribosomal subunit protein bS16
Q: Small ribosomal subunit protein uS17
R: Small ribosomal subunit protein bS18
S: Small ribosomal subunit protein uS19
T: Small ribosomal subunit protein bS20
U: Small ribosomal subunit protein bS21
a: 23S ribosomal RNA
b: 5S ribosomal RNA
c: 50S ribosomal protein L2
d: 50S ribosomal protein L3
e: Large ribosomal subunit protein uL4
f: Large ribosomal subunit protein uL5
g: Large ribosomal subunit protein uL6
h: Large ribosomal subunit protein bL9
i: Large ribosomal subunit protein uL13
j: Large ribosomal subunit protein uL14
k: Large ribosomal subunit protein uL15
l: Large ribosomal subunit protein uL16
m: Large ribosomal subunit protein bL17
n: Large ribosomal subunit protein uL18
o: Large ribosomal subunit protein bL19
p: 50S ribosomal protein L20
q: Large ribosomal subunit protein bL21
r: Large ribosomal subunit protein uL22
s: 50S ribosomal protein L23
t: 50S ribosomal protein L24
u: Large ribosomal subunit protein bL25
v: 50S ribosomal protein L27
w: 50S ribosomal protein L28
x: Large ribosomal subunit protein uL29
y: 50S ribosomal protein L30
z: 50S ribosomal protein L32
ヘテロ分子


分子量 (理論値)分子数
合計 (水以外)2,142,103342
ポリマ－2,133,12553
非ポリマー8,978289
水00
1


  • 登録構造と同一
  • 登録者・ソフトウェアが定義した集合体
  • 根拠: 電子顕微鏡法, not applicable
タイプ名称対称操作数
identity operation1_555x,y,z1

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要素

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50S ribosomal protein ... , 12種, 12分子 024cdpstvwyz

#1: タンパク質 50S ribosomal protein L33


分子量: 6388.631 Da / 分子数: 1 / 由来タイプ: 組換発現 / 由来: (組換発現) Escherichia coli (大腸菌) / 遺伝子: rpmG, rpmG_1 / 発現宿主: Escherichia coli (大腸菌) / 参照: UniProt: C3SME7
#3: タンパク質 50S ribosomal protein L35 / Large ribosomal subunit protein bL35 / Ribosomal protein A


分子量: 7313.032 Da / 分子数: 1 / 由来タイプ: 組換発現 / 由来: (組換発現) Escherichia coli (大腸菌) / 遺伝子: rpmI, b1717, JW1707 / 発現宿主: Escherichia coli (大腸菌) / 参照: UniProt: P0A7Q1
#5: タンパク質 50S ribosomal protein L31 / Large ribosomal subunit protein bL31-A


分子量: 7887.117 Da / 分子数: 1 / 由来タイプ: 組換発現 / 由来: (組換発現) Escherichia coli (大腸菌) / 遺伝子: rpmE, b3936, JW3907 / 発現宿主: Escherichia coli (大腸菌) / 参照: UniProt: P0A7M9
#30: タンパク質 50S ribosomal protein L2 / Large ribosomal subunit protein uL2


分子量: 29923.619 Da / 分子数: 1 / 由来タイプ: 組換発現 / 由来: (組換発現) Escherichia coli (大腸菌) / 遺伝子: rplB, b3317, JW3279 / 発現宿主: Escherichia coli (大腸菌) / 参照: UniProt: P60422
#31: タンパク質 50S ribosomal protein L3


分子量: 22291.562 Da / 分子数: 1 / 由来タイプ: 組換発現 / 由来: (組換発現) Escherichia coli (大腸菌) / 発現宿主: Escherichia coli (大腸菌) / 参照: UniProt: C3SQU2
#43: タンパク質 50S ribosomal protein L20


分子量: 13528.024 Da / 分子数: 1 / 由来タイプ: 組換発現 / 由来: (組換発現) Escherichia coli (大腸菌) / 遺伝子: rplT / 発現宿主: Escherichia coli (大腸菌) / 参照: UniProt: B7MAS6
#46: タンパク質 50S ribosomal protein L23


分子量: 11222.160 Da / 分子数: 1 / 由来タイプ: 組換発現 / 由来: (組換発現) Escherichia coli (大腸菌)
遺伝子: rplW, rplW_1, A5U30_004552, A8502_004023, A9X72_02185, ACN81_25520, ACU57_20960, AM464_09220, AW118_24900, AWP47_12645, B6R15_004069, B6R31_004968, BANRA_04328, BCB93_004293, BER14_25190, ...遺伝子: rplW, rplW_1, A5U30_004552, A8502_004023, A9X72_02185, ACN81_25520, ACU57_20960, AM464_09220, AW118_24900, AWP47_12645, B6R15_004069, B6R31_004968, BANRA_04328, BCB93_004293, BER14_25190, BF481_004930, BG944_001133, BGM66_003593, BJI68_08675, BJJ90_02125, BK292_20505, BK383_24645, BKL28_004543, BTB68_004356, BTQ06_10690, BvCmsKKP061_03030, BvCmsSIP010_02685, BXT93_05475, C0P57_002668, C1Q91_004904, C2121_004162, C2M16_25405, C2R31_004633, C3F40_19720, C9E67_02725, C9Z68_22635, CA593_09650, CF22_004849, CG692_20185, CG704_20870, CG831_003649, CIG67_10675, CQ986_004881, CR538_02420, CR539_21900, CR628_001366, CTR35_003630, CV83915_02780, CY655_21320, D1912_09370, D3C88_31150, D3G36_23050, D4M65_20655, D4N09_20255, D9D43_22550, D9E49_05240, D9J61_16735, DD762_23630, DIV22_15175, DNQ45_03840, DNX30_25395, DQ87_004776, DS732_24345, DTL43_20915, DU321_12085, E4K51_21575, E5H86_24440, E6D34_21675, EAI46_07560, EC95NR1_02709, ECs4183, EEP55_004492, EIA08_24040, EIZ93_12385, EN85_004349, EPS76_07130, EPS97_20010, EWK56_25080, ExPECSC038_03640, F7F11_22640, F7N46_23925, F9407_19210, F9413_21150, F9461_25570, F9B07_24600, FEJ01_22430, FIJ20_21110, FJQ40_18390, FKO60_25720, FOI11_019175, FOI11_04005, FPI65_20390, FPS11_25345, FTV92_20630, FV293_22380, FVB16_04955, FWK02_12650, FZN31_02550, FZU14_21905, G3V95_19930, G3W53_21040, G4A38_21595, G4A47_20865, G5603_24670, G8C01_22470, G9448_16990, GAI89_24745, GAJ12_24635, GGB84_004451, GJ11_21470, GKF66_21630, GNW61_16715, GOP25_22790, GP711_23390, GP954_00860, GP965_04380, GP975_01270, GP979_01920, GQA06_03865, GQM04_10650, GQM13_25285, GQM21_11315, GQN34_23350, GQW07_21465, GRO95_20730, GRW05_09145, GRW24_04670, GRW56_00380, GRW57_03455, GTP92_23110, GUC01_21265, H0O72_19500, HCQ42_003932, HEP30_018720, HEP34_004754, HHH44_004519, HI055_004156, HIE29_005203, HJQ60_004995, HLX92_09990, HLZ50_22095, HMV95_19690, HV109_02105, HV146_21755, HV209_14630, HVW04_17450, HVW43_18585, HVY77_02090, HVZ29_19180, HX086_19490, HX136_02110, I0E88_004501, I6H00_20840, I6H02_12075, IFB95_004822, IUC12_004844, J0541_004413, J5U05_004071, JNP96_25390, KV259_004342, KV317_004314, KV371_004328, KV406_004321, KV449_004356, KV455_004319, KV463_004341, KV469_004334, KV499_004405, KV500_004292, NCTC10082_02823, NCTC10089_00486, NCTC10090_03511, NCTC10418_00674, NCTC10429_01077, NCTC10764_03847, NCTC10767_01500, NCTC10865_00642, NCTC10974_00533, NCTC11112_01052, NCTC11124_04730, NCTC11126_02754, NCTC11181_02722, NCTC11341_01905, NCTC12950_00463, NCTC13148_03456, NCTC4450_02459, NCTC7927_00535, NCTC7928_02533, NCTC8009_01493, NCTC8179_05925, NCTC8333_00473, NCTC8500_00318, NCTC8621_00492, NCTC8622_00522, NCTC8959_03277, NCTC8960_03054, NCTC8985_04965, NCTC9001_04236, NCTC9044_01376, NCTC9045_00549, NCTC9073_00398, NCTC9075_00708, NCTC9081_00901, NCTC9117_00740, NCTC9702_00521, NCTC9706_02721, NCTC9962_06781, NEP60_26375, O5851_12245, RG28_21695, SAMEA3752557_01968, TUM18780_04200
発現宿主: Escherichia coli (大腸菌) / 参照: UniProt: C3SQV2
#47: タンパク質 50S ribosomal protein L24


分子量: 11339.250 Da / 分子数: 1 / 由来タイプ: 組換発現 / 由来: (組換発現) Escherichia coli (大腸菌)
遺伝子: rplX, A8502_004032, A9X72_02230, ACN81_25475, ACU57_21005, AM464_09175, AW118_24855, AWP47_12690, B6R15_004078, B6R31_004977, BANRA_04319, BCB93_004284, BER14_25145, BF481_004939, BG944_ ...遺伝子: rplX, A8502_004032, A9X72_02230, ACN81_25475, ACU57_21005, AM464_09175, AW118_24855, AWP47_12690, B6R15_004078, B6R31_004977, BANRA_04319, BCB93_004284, BER14_25145, BF481_004939, BG944_001124, BGM66_003602, BJI68_08720, BJJ90_02170, BK292_20460, BK383_24690, BKL28_004552, BTB68_004365, BTQ06_10645, BvCmsKKP061_03021, BvCmsSIP010_02676, BXT93_05520, C0P57_002659, C1Q91_004913, C2121_004171, C2M16_25450, C2R31_004624, C3F40_19765, C9E67_02770, C9Z68_22590, CA593_09695, CF22_004858, CG704_20915, CG831_003640, CIG67_10720, CQ986_004890, CR538_02465, CR539_21855, CR628_001357, CTR35_003621, CV83915_02789, CY655_21275, D4M65_20610, D9D43_22595, DD762_23585, DIV22_15220, DNQ45_03795, DNX30_25440, DQ87_004785, DS732_24300, DTL43_20960, DU321_12040, E4K51_21530, E5H86_24485, E6D34_21720, EAI46_07515, EC95NR1_02700, ECs4174, EEP55_004501, EIA08_23995, EIZ93_12430, EN85_004358, EPS97_20055, ExPECSC038_03649, F7F11_22685, F7N46_23970, F9407_19255, F9413_21195, F9461_25615, F9B07_24645, FEJ01_22475, FIJ20_21155, FJQ40_18345, FKO60_25675, FOI11_019220, FOI11_03960, FPS11_25390, FTV92_20675, FTV93_20860, FV293_22425, FVB16_04910, FWK02_06310, FZN31_02595, FZU14_21860, G3V95_19885, G4A38_21550, G4A47_20820, G5603_24625, G8C01_22515, G9448_17035, GAI89_24790, GAJ12_24680, GJ11_21425, GKF66_21585, GNW61_16670, GOP25_22745, GP954_00905, GP965_04335, GP975_01225, GP979_01965, GQA06_03910, GQM04_10605, GQM13_25240, GQM21_11270, GQW07_21510, GRO95_20775, GRW05_09100, GRW24_04715, GRW56_00335, GRW57_03500, GTP92_23155, GUC01_21220, H0O72_19455, HCQ42_003941, HEP30_018675, HEP34_004763, HHH44_004528, HI055_004147, HJQ60_005004, HLX92_10035, HLZ50_22140, HMV95_19645, HV109_02150, HV146_21710, HV209_14675, HVW04_17495, HVW43_18630, HVY77_02135, HVZ29_19135, HX086_19445, HX136_02155, I0E88_004510, I6H00_20885, I6H02_12030, IUC12_004853, J0541_004404, J5U05_004080, JNP96_25345, KV259_004351, KV317_004323, KV371_004337, KV406_004330, KV449_004365, KV455_004328, KV463_004350, KV469_004343, KV499_004414, KV500_004301, NCTC10082_02832, NCTC10418_00684, NCTC10764_03856, NCTC10865_00652, NCTC10974_00542, NCTC11124_04721, NCTC11181_02731, NCTC11341_01896, NCTC12950_00472, NCTC7922_04049, NCTC7927_00544, NCTC8009_01504, NCTC8179_05935, NCTC8333_00482, NCTC8500_00327, NCTC8621_00501, NCTC8622_00513, NCTC8960_03063, NCTC8985_04975, NCTC9044_01365, NCTC9045_00558, NCTC9077_00597, NCTC9117_00749, NCTC9702_00530, NCTC9706_02730, NEP60_26330, O5851_12290, RG28_21650, SAMEA3752557_01959, TUM18780_04290, WR15_19645
発現宿主: Escherichia coli (大腸菌) / 参照: UniProt: C3SQZ7
#49: タンパク質 50S ribosomal protein L27 / Large ribosomal subunit protein bL27


分子量: 9146.540 Da / 分子数: 1 / 由来タイプ: 組換発現 / 由来: (組換発現) Escherichia coli (大腸菌) / 遺伝子: rpmA, b3185, JW3152 / 発現宿主: Escherichia coli (大腸菌) / 参照: UniProt: P0A7L8
#50: タンパク質 50S ribosomal protein L28


分子量: 9027.551 Da / 分子数: 1 / 由来タイプ: 組換発現 / 由来: (組換発現) Escherichia coli (大腸菌) / 遺伝子: rpmB, ECVG_03476 / 発現宿主: Escherichia coli (大腸菌) / 参照: UniProt: A0A1X3JA73
#52: タンパク質 50S ribosomal protein L30


分子量: 6554.820 Da / 分子数: 1 / 由来タイプ: 組換発現 / 由来: (組換発現) Escherichia coli (大腸菌)
遺伝子: rpmD, A5U30_004536, A6592_25715, A8499_003316, A8502_004039, A9X72_02265, AC065_15925, ACN81_25440, ACU57_21040, AM464_09140, APX88_16155, AW118_24820, AWP47_12725, B6R12_002823, B6R15_ ...遺伝子: rpmD, A5U30_004536, A6592_25715, A8499_003316, A8502_004039, A9X72_02265, AC065_15925, ACN81_25440, ACU57_21040, AM464_09140, APX88_16155, AW118_24820, AWP47_12725, B6R12_002823, B6R15_004085, B6R17_004958, B6R31_004984, B6R48_005051, B6R87_004961, BANRA_04312, BB540_01660, BER14_25110, BF481_004946, BG944_001117, BGM66_003609, BHS81_19755, BJI68_08755, BJJ90_02205, BK292_20425, BK383_24725, BKL28_004559, BMT50_12295, BMT91_19990, BR158_003774, BTB68_004372, BTQ06_10610, BUO55_004713, BvCmsKKP061_03014, BvCmsSIP010_02669, BXT93_05555, BZL69_25950, C0P57_002652, C1Q91_004920, C2121_004178, C2M16_25485, C2R31_004617, C3F40_19800, C5N07_23570, C9E67_02805, C9Z68_22555, CA593_09730, CCS08_00135, CCU84_002918, CDL36_25590, CF22_004865, CG692_20265, CG704_20950, CG831_003633, CIG67_10755, CQ986_004897, CQB02_00630, CR538_02500, CR539_21820, CR628_001350, CRM83_22925, CTR35_003614, CV83915_02796, CWS33_24860, CX938_003815, CY655_21240, D1912_11425, D3G36_23130, D4M65_20575, D4N09_20175, D6T60_05695, D9D43_22630, D9E34_23605, D9E49_05160, D9H94_20830, D9J03_20055, D9J61_16655, DD762_23550, DIV22_15255, DNQ45_03760, DNX30_25475, DS732_24265, DTL43_20995, DTL90_24695, DTM45_24550, DU321_12005, E0I52_20145, E4K51_21495, E5H86_24520, E6D34_21755, EA239_22795, EAI46_07480, EB534_RS01705, EC95NR1_02693, ECs4167, EIA08_23960, EIZ93_12465, EN85_004365, EPS76_07210, EPS97_20090, ERS085406_04406, ERS139208_04354, EWK56_25000, ExPECSC038_03656, F3P10_05565, F7F11_22720, F7N46_24005, F9413_21230, F9461_25650, F9B07_24680, FA868_22750, FEJ01_22510, FFF58_25450, FGG80_25130, FHD44_21270, FHQ97_20115, FIJ20_21190, FJQ40_18310, FKO60_25640, FN993_004711, FOI11_019255, FOI11_03925, FPI65_20310, FPS11_25425, FTV92_20710, FTV93_20895, FV293_22460, FVB16_04875, FWK02_06275, FZN31_02630, FZU14_21825, G3V95_19850, G3W53_20960, G4A38_21515, G4A47_20785, G5603_24590, G9448_17070, GAI89_24825, GAJ12_24715, GAJ26_22015, GGB84_004467, GIB53_19395, GJ11_21390, GKF52_21245, GKF66_21550, GKF89_18475, GNW61_16635, GOP14_004273, GOP25_22710, GP711_23310, GP954_00940, GP965_04300, GP975_01190, GP979_02000, GQA06_03945, GQM04_10570, GQM13_25205, GQM17_19920, GQM21_11235, GQN34_23270, GQW07_21545, GRO95_20810, GRW05_09065, GRW24_04750, GRW56_00300, GRW57_03535, GSM54_23325, GSY44_20005, GUC01_21185, GUI33_20050, H0O53_20825, H0O72_19420, H0P11_20920, H5Z35_004789, H6Y26_003164, HCQ42_003948, HEP30_018640, HEP34_004770, HHH44_004535, HI055_004140, HIE29_005187, HJY54_004753, HKA49_005035, HLQ92_22595, HLV18_23335, HLX92_10070, HLZ50_22175, HMV95_19610, HMW38_23110, HV109_02185, HV146_21675, HV209_14710, HVV39_09270, HVW04_17530, HVW43_18665, HVY77_02170, HVZ29_19100, HVZ30_02050, HVZ71_02355, HX136_02190, I6H00_20920, I6H02_11995, IFB95_004806, IUD45_003540, J0541_004397, J4S20_004802, J5U05_004087, J7T50_001453, JFD_01884, JFD_03713, JNP96_25310, KQ660_11775, NCTC10082_02839, NCTC10089_00502, NCTC10090_03527, NCTC10418_00690, NCTC10429_01095, NCTC10487_00445, NCTC10764_03863, NCTC10767_01516, NCTC10865_00661, NCTC10974_00551, NCTC11126_02771, NCTC11127_03223, NCTC11181_02738, NCTC11341_01889, NCTC12950_00479, NCTC13127_00786, NCTC4450_02475, NCTC7922_04042, NCTC7927_00551, NCTC7928_02516, NCTC8009_01511, NCTC8179_05942, NCTC8333_00489, NCTC8450_04937, NCTC8500_00335, NCTC8621_00508, NCTC8622_00507, NCTC8959_03261, NCTC8960_03070, NCTC8985_04982, NCTC9001_04254, NCTC9044_01356, NCTC9045_00566, NCTC9050_03611, NCTC9071_00483, NCTC9073_00380, NCTC9075_00724, NCTC9077_00604, NCTC9081_00918, NCTC9111_00867, NCTC9117_00756, NCTC9702_00537, NCTC9706_02737, NCTC9775_04003, NCTC9777_01891, NEP60_26295, OS110_25120, RG28_21615, SAMEA3472044_01162, SAMEA3472056_05411, SAMEA3752557_01952, SAMEA3753106_04634, TUM18780_04360, WR15_19610
発現宿主: Escherichia coli (大腸菌) / 参照: UniProt: C3SR32
#53: タンパク質 50S ribosomal protein L32


分子量: 6463.445 Da / 分子数: 1 / 由来タイプ: 組換発現 / 由来: (組換発現) Escherichia coli (大腸菌)
遺伝子: rpmF, rpmF_1, A5U30_002830, A8502_000661, A9X72_15435, ACN81_09130, ACU57_04225, AM464_23480, AW118_12110, AWP47_13180, B6R15_001792, B6R31_003138, BANRA_01624, BCB93_002043, BER14_10210, ...遺伝子: rpmF, rpmF_1, A5U30_002830, A8502_000661, A9X72_15435, ACN81_09130, ACU57_04225, AM464_23480, AW118_12110, AWP47_13180, B6R15_001792, B6R31_003138, BANRA_01624, BCB93_002043, BER14_10210, BF481_002131, BG944_003607, BGM66_002729, BJI68_20640, BJJ90_15780, BK292_02505, BK383_03640, BKL28_002219, BTB68_003153, BTQ06_08080, BvCmsKKP061_02173, BvCmsSIP010_04163, BXT93_21825, C0P57_001747, C1Q91_003167, C2121_002257, C2M16_23600, C2R31_002348, C3F40_06530, C9E67_18185, C9Z68_07340, CA593_23040, CF22_003088, CG692_05970, CG704_04105, CG831_001542, CIG67_21935, CQ986_001084, CR538_15635, CR539_10135, CR628_002785, CTR35_002374, CV83915_00494, CY655_06595, D1912_08350, D3C88_19355, D3G36_11860, D4M65_04795, D4N09_11015, D9D43_11825, D9E49_23135, D9J61_04035, DD762_03625, DIV22_29035, DNQ45_10015, DNX30_12140, DQ87_002042, DS732_10505, DTL43_16270, DU321_22840, E4K51_18340, E5H86_12535, E6D34_10935, EAI46_03975, EC95NR1_05485, ECs1467, EEP55_000822, EIA08_02925, EIZ93_13150, EN85_002840, EPS76_00770, EPS97_06370, EWK56_20060, ExPECSC038_03490, F7F11_10320, F7N46_02450, F9413_18150, F9461_13585, F9B07_16900, FEJ01_12565, FIJ20_07300, FJQ40_00940, FKO60_17370, FOI11_007500, FOI11_14470, FPI65_06540, FPS11_20235, FTV92_08325, FTV93_08485, FV293_19565, FVB16_04695, FWK02_15570, FZN31_14620, FZU14_20010, G3V95_08050, G3W53_15755, G4A38_16050, G4A47_15410, G5603_00600, G8C01_10030, G9448_05130, GAI89_13280, GAJ12_13930, GGB84_000483, GJ11_06555, GKF66_05180, GNW61_10640, GOP25_17405, GP711_16475, GP954_12815, GP965_04045, GP975_03585, GP979_13150, GQA06_08430, GQM04_02110, GQM13_14340, GQM21_06935, GQN34_10245, GQW07_08880, GRO95_10420, GRW05_02410, GRW24_27585, GRW56_06550, GRW57_07595, GTP92_19120, GUC01_01680, H0O72_16850, HCQ42_001914, HEP30_011955, HEP34_001075, HHH44_003019, HI055_001485, HIE29_000052, HJQ60_005092, HLX92_03690, HLZ50_01830, HMV95_16370, HV109_14610, HV146_09250, HV209_29060, HVV39_00410, HVW04_05590, HVW43_06430, HVY77_15765, HVZ29_08715, HX086_07035, HX136_15650, I0E88_003516, I6H00_08540, I6H02_24530, IFB95_003696, IUC12_000121, J0541_003169, J5U05_002959, JNP96_10710, KV259_001392, KV317_003230, KV371_000953, KV406_001102, KV449_001342, KV455_003227, KV463_001049, KV469_000542, KV499_000679, KV500_001199, NCTC10082_00641, NCTC10089_03005, NCTC10090_00680, NCTC10418_04509, NCTC10764_02647, NCTC10767_05044, NCTC10865_03684, NCTC10974_03416, NCTC11126_00919, NCTC11181_00164, NCTC11341_04894, NCTC12950_03296, NCTC13148_05706, NCTC4450_04127, NCTC7922_00634, NCTC7927_03385, NCTC7928_00455, NCTC8009_05657, NCTC8179_02874, NCTC8333_03590, NCTC8621_03238, NCTC8622_05813, NCTC8959_00826, NCTC8960_00763, NCTC8985_01747, NCTC9044_04397, NCTC9045_03550, NCTC9073_01975, NCTC9075_04022, NCTC9077_03763, NCTC9081_03511, NCTC9117_03837, NCTC9706_00412, NCTC9962_06984, NEP60_22810, O5851_21660, RG28_16890, SAMEA3752557_01555, TUM18780_25170, WR15_23855
発現宿主: Escherichia coli (大腸菌) / 参照: UniProt: C3TE02

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Large ribosomal subunit protein ... , 17種, 17分子 13efghijklmnoqrux

#2: タンパク質・ペプチド Large ribosomal subunit protein bL34 / 50S ribosomal protein L34


分子量: 5397.463 Da / 分子数: 1 / 由来タイプ: 組換発現 / 由来: (組換発現) Escherichia coli (大腸菌) / 遺伝子: rpmH, rimA, ssaF, b3703, JW3680 / 発現宿主: Escherichia coli (大腸菌) / 参照: UniProt: P0A7P5
#4: タンパク質・ペプチド Large ribosomal subunit protein bL36A / 50S ribosomal protein L36 / Ribosomal protein B


分子量: 4377.390 Da / 分子数: 1 / 由来タイプ: 組換発現 / 由来: (組換発現) Escherichia coli (大腸菌) / 遺伝子: rpmJ, b3299, JW3261 / 発現宿主: Escherichia coli (大腸菌) / 参照: UniProt: P0A7Q6
#32: タンパク質 Large ribosomal subunit protein uL4 / 50S ribosomal protein L4


分子量: 22121.566 Da / 分子数: 1 / 由来タイプ: 組換発現 / 由来: (組換発現) Escherichia coli (大腸菌) / 遺伝子: rplD, eryA, b3319, JW3281 / 発現宿主: Escherichia coli (大腸菌) / 参照: UniProt: P60723
#33: タンパク質 Large ribosomal subunit protein uL5 / 50S ribosomal protein L5


分子量: 20333.611 Da / 分子数: 1 / 由来タイプ: 組換発現 / 由来: (組換発現) Escherichia coli (大腸菌) / 遺伝子: rplE, b3308, JW3270 / 発現宿主: Escherichia coli (大腸菌) / 参照: UniProt: P62399
#34: タンパク質 Large ribosomal subunit protein uL6 / 50S ribosomal protein L6


分子量: 18932.791 Da / 分子数: 1 / 由来タイプ: 組換発現 / 由来: (組換発現) Escherichia coli (大腸菌) / 遺伝子: rplF, b3305, JW3267 / 発現宿主: Escherichia coli (大腸菌) / 参照: UniProt: P0AG55
#35: タンパク質 Large ribosomal subunit protein bL9 / 50S ribosomal protein L9


分子量: 15789.020 Da / 分子数: 1 / 由来タイプ: 組換発現 / 由来: (組換発現) Escherichia coli (大腸菌) / 遺伝子: rplI, b4203, JW4161 / 発現宿主: Escherichia coli (大腸菌) / 参照: UniProt: P0A7R1
#36: タンパク質 Large ribosomal subunit protein uL13 / 50S ribosomal protein L13


分子量: 16050.606 Da / 分子数: 1 / 由来タイプ: 組換発現 / 由来: (組換発現) Escherichia coli (大腸菌) / 遺伝子: rplM, b3231, JW3200 / 発現宿主: Escherichia coli (大腸菌) / 参照: UniProt: P0AA10
#37: タンパク質 Large ribosomal subunit protein uL14 / 50S ribosomal protein L14


分子量: 13565.067 Da / 分子数: 1 / 由来タイプ: 組換発現 / 由来: (組換発現) Escherichia coli (大腸菌) / 遺伝子: rplN, b3310, JW3272 / 発現宿主: Escherichia coli (大腸菌) / 参照: UniProt: P0ADY3
#38: タンパク質 Large ribosomal subunit protein uL15 / 50S ribosomal protein L15


分子量: 15008.471 Da / 分子数: 1 / 由来タイプ: 組換発現 / 由来: (組換発現) Escherichia coli (大腸菌) / 遺伝子: rplO, b3301, JW3263 / 発現宿主: Escherichia coli (大腸菌) / 参照: UniProt: P02413
#39: タンパク質 Large ribosomal subunit protein uL16 / 50S ribosomal protein L16


分子量: 15343.327 Da / 分子数: 1 / 由来タイプ: 組換発現 / 由来: (組換発現) Escherichia coli (大腸菌) / 遺伝子: rplP, EcE24377A_3796 / 発現宿主: Escherichia coli (大腸菌) / 参照: UniProt: A7ZSK2
#40: タンパク質 Large ribosomal subunit protein bL17 / 50S ribosomal protein L17


分子量: 14393.657 Da / 分子数: 1 / 由来タイプ: 組換発現 / 由来: (組換発現) Escherichia coli (大腸菌) / 遺伝子: rplQ, b3294, JW3256 / 発現宿主: Escherichia coli (大腸菌) / 参照: UniProt: P0AG44
#41: タンパク質 Large ribosomal subunit protein uL18 / 50S ribosomal protein L18


分子量: 12794.668 Da / 分子数: 1 / 由来タイプ: 組換発現 / 由来: (組換発現) Escherichia coli (大腸菌) / 遺伝子: rplR, b3304, JW3266 / 発現宿主: Escherichia coli (大腸菌) / 参照: UniProt: P0C018
#42: タンパク質 Large ribosomal subunit protein bL19


分子量: 13159.278 Da / 分子数: 1 / 由来タイプ: 組換発現 / 由来: (組換発現) Escherichia coli (大腸菌) / 遺伝子: rplS / 発現宿主: Escherichia coli (大腸菌) / 参照: UniProt: P0A7K8
#44: タンパク質 Large ribosomal subunit protein bL21 / 50S ribosomal protein L21


分子量: 11586.374 Da / 分子数: 1 / 由来タイプ: 組換発現 / 由来: (組換発現) Escherichia coli (大腸菌) / 遺伝子: rplU, b3186, JW3153 / 発現宿主: Escherichia coli (大腸菌) / 参照: UniProt: P0AG48
#45: タンパク質 Large ribosomal subunit protein uL22 / 50S ribosomal protein L22


分子量: 12253.359 Da / 分子数: 1 / 由来タイプ: 組換発現 / 由来: (組換発現) Escherichia coli (大腸菌) / 遺伝子: rplV, eryB, b3315, JW3277 / 発現宿主: Escherichia coli (大腸菌) / 参照: UniProt: P61175
#48: タンパク質 Large ribosomal subunit protein bL25 / 50S ribosomal protein L25


分子量: 10713.465 Da / 分子数: 1 / 由来タイプ: 組換発現 / 由来: (組換発現) Escherichia coli (大腸菌) / 遺伝子: rplY, b2185, JW2173 / 発現宿主: Escherichia coli (大腸菌) / 参照: UniProt: P68919
#51: タンパク質 Large ribosomal subunit protein uL29


分子量: 7286.464 Da / 分子数: 1 / 由来タイプ: 組換発現 / 由来: (組換発現) Escherichia coli (大腸菌)
遺伝子: rpmC, A5U30_004546, A8502_004029, ACN81_25490, ACU57_20990, AW118_24870, AWP47_12675, B6R15_004075, B6R31_004974, BANRA_01943, BANRA_04322, BE932_15505, BER14_25160, BG944_001127, BGM66_ ...遺伝子: rpmC, A5U30_004546, A8502_004029, ACN81_25490, ACU57_20990, AW118_24870, AWP47_12675, B6R15_004075, B6R31_004974, BANRA_01943, BANRA_04322, BE932_15505, BER14_25160, BG944_001127, BGM66_003599, BGZ_02596, BGZ_04119, BJI68_08705, BK292_20475, BK383_24675, BKL28_004549, BTB68_004362, BTQ06_10660, BvCmsKKP061_03024, BvCmsSIP010_02679, BXT93_05505, C0P57_002662, C1Q91_004910, C2121_004168, C2M16_25435, C2R31_004627, C3F40_19750, C9Z68_22605, CF22_004855, CG704_20900, CG831_003643, CIG67_10705, CTR35_003624, CV83915_02786, D3G36_23080, D4M65_20625, D4N09_20225, D9D43_22580, D9E49_05210, D9J61_16705, DD762_23600, DIV22_15205, DNQ45_03810, DNX30_25425, DS732_24315, DTL43_20945, DU321_12055, E4K51_21545, E5H86_24470, E6D34_21705, EAI46_07530, ECs4177, EIA08_24010, EIMP300_17750, EIZ93_12415, EN85_004355, EPS76_07160, EPS97_20040, EWK56_25050, ExPECSC038_03646, F7F11_22670, F7N46_23955, F9413_21180, F9461_25600, F9B07_24630, FGAF848_25910, FIJ20_21140, FJQ40_18360, FKO60_25690, FOI11_019205, FOI11_03975, FORC44_0486, FPI65_20360, FPS11_25375, FTV93_20845, FVB16_04925, FWK02_06325, FZU14_21875, G3V95_19900, G3W53_21010, G4A38_21565, G4A47_20835, G5603_24640, GAI89_24775, GAJ12_24665, GKF66_21600, GNW61_16685, GOP25_22760, GP711_23360, GP954_00890, GP965_04350, GP975_01240, GP979_01950, GQA06_03895, GQE86_20135, GQM04_10620, GQM13_25255, GQM21_11285, GQN34_23320, GQW07_21495, GRC73_21695, GRO95_20760, GRW05_09115, GRW24_04700, GRW56_00350, GRW57_03485, GUC01_21235, H0O72_19470, HEP30_018690, HEP34_004760, HHH44_004525, HI055_004150, HIE29_005197, HJQ60_005001, HLX92_10020, HLZ50_22125, HMV95_19660, HV109_02135, HV209_14660, HVV39_09320, HVW04_17480, HVW43_18615, HVY77_02120, I6H00_20870, I6H02_12045, IFB95_004816, J0541_004407, J5U05_004077, JNP96_25360, KV499_004411, NCTC10082_02829, NCTC10089_00492, NCTC10418_00681, NCTC10429_01084, NCTC10764_03853, NCTC10767_01506, NCTC10865_00649, NCTC10974_00539, NCTC11126_02760, NCTC11181_02728, NCTC11341_01899, NCTC7922_04052, NCTC7927_00541, NCTC7928_02527, NCTC8009_01501, NCTC8179_05932, NCTC8333_00479, NCTC8500_00324, NCTC8621_00498, NCTC8622_00516, NCTC8959_03271, NCTC8960_03060, NCTC8985_04972, NCTC9001_04244, NCTC9044_01368, NCTC9045_00555, NCTC9075_00714, NCTC9077_00594, NCTC9081_00907, NCTC9117_00746, NCTC9702_00527, NCTC9706_02727, NCTC9962_06787, NEP60_26345, OGM49_22385, QDW62_02140, RZR61_12945, SAMEA3472112_00683, SAMEA3752557_01962, WR15_19660
発現宿主: Escherichia coli (大腸菌) / 参照: UniProt: C3SQY2

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RNA鎖 , 4種, 4分子 5Aab

#6: RNA鎖 E-site tRNA


分子量: 589.430 Da / 分子数: 1 / 由来タイプ: 組換発現 / 由来: (組換発現) Escherichia coli (大腸菌) / 発現宿主: Escherichia coli (大腸菌)
#7: RNA鎖 16S ribosomal RNA


分子量: 491856.719 Da / 分子数: 1 / 由来タイプ: 組換発現 / 由来: (組換発現) Escherichia coli (大腸菌) / 発現宿主: Escherichia coli (大腸菌)
#28: RNA鎖 23S ribosomal RNA


分子量: 941811.562 Da / 分子数: 1 / 由来タイプ: 組換発現 / 由来: (組換発現) Escherichia coli (大腸菌) / 発現宿主: Escherichia coli (大腸菌)
#29: RNA鎖 5S ribosomal RNA


分子量: 38790.090 Da / 分子数: 1 / 由来タイプ: 組換発現 / 由来: (組換発現) Escherichia coli (大腸菌) / 発現宿主: Escherichia coli (大腸菌) / 参照: GenBank: 1273279017

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Small ribosomal subunit protein ... , 18種, 18分子 BDEFGHIJKMNOPQRSTU

#8: タンパク質 Small ribosomal subunit protein uS2 / 30S ribosomal protein S2


分子量: 26781.670 Da / 分子数: 1 / 由来タイプ: 組換発現 / 由来: (組換発現) Escherichia coli (大腸菌) / 遺伝子: rpsB, b0169, JW0164 / 発現宿主: Escherichia coli (大腸菌) / 参照: UniProt: P0A7V0
#10: タンパク質 Small ribosomal subunit protein uS4


分子量: 23514.199 Da / 分子数: 1 / 由来タイプ: 組換発現 / 由来: (組換発現) Escherichia coli (大腸菌) / 遺伝子: rpsD, ramA, b3296, JW3258 / 発現宿主: Escherichia coli (大腸菌) / 参照: UniProt: P0A7V8
#11: タンパク質 Small ribosomal subunit protein uS5


分子量: 17629.398 Da / 分子数: 1 / 由来タイプ: 組換発現 / 由来: (組換発現) Escherichia coli (大腸菌) / 遺伝子: rpsE, spc, b3303, JW3265 / 発現宿主: Escherichia coli (大腸菌) / 参照: UniProt: P0A7W1
#12: タンパク質 Small ribosomal subunit protein bS6


分子量: 15727.512 Da / 分子数: 1 / 由来タイプ: 組換発現 / 由来: (組換発現) Escherichia coli (大腸菌) / 遺伝子: rpsF, b4200, JW4158 / 発現宿主: Escherichia coli (大腸菌) / 参照: UniProt: P02358
#13: タンパク質 Small ribosomal subunit protein uS7


分子量: 20055.156 Da / 分子数: 1 / 由来タイプ: 組換発現 / 由来: (組換発現) Escherichia coli (大腸菌) / 遺伝子: rpsG, b3341, JW3303 / 発現宿主: Escherichia coli (大腸菌) / 参照: UniProt: P02359
#14: タンパク質 Small ribosomal subunit protein uS8


分子量: 14146.557 Da / 分子数: 1 / 由来タイプ: 組換発現 / 由来: (組換発現) Escherichia coli (大腸菌) / 遺伝子: rpsH / 発現宿主: Escherichia coli (大腸菌) / 参照: UniProt: C3SR12
#15: タンパク質 Small ribosomal subunit protein uS9


分子量: 14886.270 Da / 分子数: 1 / 由来タイプ: 組換発現 / 由来: (組換発現) Escherichia coli (大腸菌) / 遺伝子: rpsI / 発現宿主: Escherichia coli (大腸菌) / 参照: UniProt: C3SRY2
#16: タンパク質 Small ribosomal subunit protein uS10


分子量: 11755.597 Da / 分子数: 1 / 由来タイプ: 組換発現 / 由来: (組換発現) Escherichia coli (大腸菌) / 遺伝子: rpsJ / 発現宿主: Escherichia coli (大腸菌) / 参照: UniProt: C3SQT7
#17: タンパク質 Small ribosomal subunit protein uS11 / 30S ribosomal protein S11


分子量: 13887.958 Da / 分子数: 1 / 由来タイプ: 組換発現 / 由来: (組換発現) Escherichia coli (大腸菌) / 遺伝子: rpsK, EcE24377A_3780 / 発現宿主: Escherichia coli (大腸菌) / 参照: UniProt: A7ZSI6
#19: タンパク質 Small ribosomal subunit protein uS13


分子量: 13128.467 Da / 分子数: 1 / 由来タイプ: 組換発現 / 由来: (組換発現) Escherichia coli (大腸菌) / 遺伝子: rpsM, G711_03878 / 発現宿主: Escherichia coli (大腸菌) / 参照: UniProt: A0A7U9IV78
#20: タンパク質 Small ribosomal subunit protein uS14


分子量: 11606.560 Da / 分子数: 1 / 由来タイプ: 組換発現 / 由来: (組換発現) Escherichia coli (大腸菌) / 遺伝子: rpsN, NCTC9073_00385 / 発現宿主: Escherichia coli (大腸菌) / 参照: UniProt: A0A2X1PQW4
#21: タンパク質 Small ribosomal subunit protein uS15


分子量: 10290.816 Da / 分子数: 1 / 由来タイプ: 組換発現 / 由来: (組換発現) Escherichia coli (大腸菌) / 遺伝子: rpsO / 発現宿主: Escherichia coli (大腸菌) / 参照: UniProt: C3SSQ7
#22: タンパク質 Small ribosomal subunit protein bS16 / 30S ribosomal protein S16


分子量: 9207.572 Da / 分子数: 1 / 由来タイプ: 組換発現 / 由来: (組換発現) Escherichia coli (大腸菌) / 遺伝子: rpsP, b2609, JW2590 / 発現宿主: Escherichia coli (大腸菌) / 参照: UniProt: P0A7T3
#23: タンパク質 Small ribosomal subunit protein uS17


分子量: 9724.491 Da / 分子数: 1 / 由来タイプ: 組換発現 / 由来: (組換発現) Escherichia coli (大腸菌) / 遺伝子: rpsQ / 発現宿主: Escherichia coli (大腸菌) / 参照: UniProt: C3SQY7
#24: タンパク質 Small ribosomal subunit protein bS18


分子量: 9005.472 Da / 分子数: 1 / 由来タイプ: 組換発現 / 由来: (組換発現) Escherichia coli (大腸菌) / 遺伝子: rpsR / 発現宿主: Escherichia coli (大腸菌) / 参照: UniProt: A0A0E2KXL3
#25: タンパク質 Small ribosomal subunit protein uS19


分子量: 10455.355 Da / 分子数: 1 / 由来タイプ: 組換発現 / 由来: (組換発現) Escherichia coli (大腸菌) / 遺伝子: rpsS / 発現宿主: Escherichia coli (大腸菌) / 参照: UniProt: C3SQW2
#26: タンパク質 Small ribosomal subunit protein bS20 / 30S ribosomal protein S20


分子量: 9712.476 Da / 分子数: 1 / 由来タイプ: 組換発現 / 由来: (組換発現) Escherichia coli (大腸菌) / 遺伝子: rpsT, EcE24377A_0023 / 発現宿主: Escherichia coli (大腸菌) / 参照: UniProt: A7ZHB2
#27: タンパク質 Small ribosomal subunit protein bS21


分子量: 8524.039 Da / 分子数: 1 / 由来タイプ: 組換発現 / 由来: (組換発現) Escherichia coli (大腸菌) / 遺伝子: rpsU / 発現宿主: Escherichia coli (大腸菌) / 参照: UniProt: A0A0E2L2J1

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30S ribosomal protein ... , 2種, 2分子 CL

#9: タンパク質 30S ribosomal protein S3


分子量: 26031.316 Da / 分子数: 1 / 由来タイプ: 組換発現 / 由来: (組換発現) Escherichia coli (大腸菌) / 発現宿主: Escherichia coli (大腸菌) / 参照: UniProt: P0A7V3
#18: タンパク質 30S ribosomal protein S12 / Small ribosomal subunit protein uS12


分子量: 13814.249 Da / 分子数: 1 / 由来タイプ: 組換発現 / 由来: (組換発現) Escherichia coli (大腸菌) / 遺伝子: rpsL, strA, b3342, JW3304 / 発現宿主: Escherichia coli (大腸菌) / 参照: UniProt: P0A7S3

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非ポリマー , 4種, 289分子

#54: 化合物 ChemComp-ZN / ZINC ION


分子量: 65.409 Da / 分子数: 2 / 由来タイプ: 合成 / 式: Zn
#55: 化合物...
ChemComp-MG / MAGNESIUM ION


分子量: 24.305 Da / 分子数: 272 / 由来タイプ: 合成 / 式: Mg
#56: 化合物
ChemComp-SPD / SPERMIDINE / N-(2-AMINO-PROPYL)-1,4-DIAMINOBUTANE / PA(34)


分子量: 145.246 Da / 分子数: 14 / 由来タイプ: 合成 / 式: C7H19N3
#57: 化合物 ChemComp-SPM / SPERMINE


分子量: 202.340 Da / 分子数: 1 / 由来タイプ: 合成 / 式: C10H26N4

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詳細

研究の焦点であるリガンドがあるかN
Has protein modificationY

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実験情報

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実験

実験手法: 電子顕微鏡法
EM実験試料の集合状態: PARTICLE / ３次元再構成法: 単粒子再構成法

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試料調製

構成要素名称: Chimeric Escherichia coli 70S ribosome containing an evolved Vibrio cholerae 16S rRNA (VC-S4.4)
タイプ: RIBOSOME / Entity ID: #1-#53 / 由来: RECOMBINANT
由来(天然)生物種: Escherichia coli (大腸菌)
由来(組換発現)生物種: Escherichia coli (大腸菌)
緩衝液pH: 7.4
試料包埋: NO / シャドウイング: NO / 染色: NO / 凍結: YES
急速凍結凍結剤: ETHANE

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電子顕微鏡撮影

顕微鏡モデル: TFS GLACIOS
電子銃電子線源: FIELD EMISSION GUN / 加速電圧: 200 kV / 照射モード: FLOOD BEAM
電子レンズモード: BRIGHT FIELD / 最大 デフォーカス（公称値）: 2500 nm / 最小 デフォーカス（公称値）: 500 nm
撮影電子線照射量: 60 e/Å2
フィルム・検出器のモデル: FEI FALCON IV (4k x 4k)

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解析

EMソフトウェア
ID名称カテゴリ
1cisTEM粒子像選択
13cisTEM３次元再構成
CTF補正タイプ: PHASE FLIPPING AND AMPLITUDE CORRECTION
3次元再構成解像度: 3 Å / 解像度の算出法: FSC 0.143 CUT-OFF / 粒子像の数: 450287 / 対称性のタイプ: POINT

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万見について

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お知らせ

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2022年2月9日: EMDBエントリの付随情報ファイルのフォーマットが新しくなりました

EMDBエントリの付随情報ファイルのフォーマットが新しくなりました

  • EMDBのヘッダファイルのバージョン3が、公式のフォーマットとなりました。
  • これまでは公式だったバージョン1.9は、アーカイブから削除されます。

関連情報:EMDBヘッダ

外部リンク:wwPDBはEMDBデータモデルのバージョン3へ移行します

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2020年8月12日: 新型コロナ情報

新型コロナ情報

URL: https://pdbj.org/emnavi/covid19.php

新ページ: EM Navigatorに新型コロナウイルスの特設ページを開設しました。

関連情報:Covid-19情報 / 2020年3月5日: 新型コロナウイルスの構造データ

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2020年3月5日: 新型コロナウイルスの構造データ

新型コロナウイルスの構造データ

関連情報:万見生物種 / 2020年8月12日: 新型コロナ情報

外部リンク:COVID-19特集ページ - PDBj / 今月の分子2020年2月：コロナウイルスプロテーアーゼ

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2019年1月31日: EMDBのIDの桁数の変更

EMDBのIDの桁数の変更

  • EMDBエントリに付与されているアクセスコード(EMDB-ID)は4桁の数字(例、EMD-1234)でしたが、間もなく枯渇します。これまでの4桁のID番号は4桁のまま変更されませんが、4桁の数字を使い切った後に発行されるIDは5桁以上の数字(例、EMD-12345)になります。5桁のIDは2019年の春頃から発行される見通しです。
  • EM Navigator/万見では、接頭語「EMD-」は省略されています。

関連情報:Q: 「EMD」とは何ですか？ / 万見/EM NavigatorにおけるID/アクセスコードの表記

外部リンク:EMDB Accession Codes are Changing Soon! / PDBjへお問い合わせ

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2017年7月12日: PDB大規模アップデート

PDB大規模アップデート

  • 新バージョンのPDBx/mmCIF辞書形式に基づくデータがリリースされました。
  • 今回の更新はバージョン番号が4から5になる大規模なもので、全エントリデータの書き換えが行われる「Remediation」というアップデートに該当します。
  • このバージョンアップで、電子顕微鏡の実験手法に関する多くの項目の書式が改定されました(例：em_softwareなど)。
  • EM NavigatorとYorodumiでも、この改定に基づいた表示内容になります。

外部リンク:wwPDB Remediation / OneDepデータ基準に準拠した、より強化された内容のモデル構造ファイルが、PDBアーカイブで公開されました。

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万見 (Yorodumi)

幾万の構造データを、幾万の視点から

  • 万見(Yorodumi)は、EMDB/PDB/SASBDBなどの構造データを閲覧するためのページです。
  • EM Navigatorの詳細ページの後継、Omokage検索のフロントエンドも兼ねています。

関連情報:EMDB / PDB / SASBDB / 3つのデータバンクの比較 / 万見検索 / 2016年8月31日: 新しいEM Navigatorと万見 / 万見文献 / Jmol/JSmol / 機能・相同性情報 / 新しいEM Navigatorと万見の変更点

他の情報も見る