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基本情報
登録情報 | データベース: EMDB / ID: EMD-9955 | ||||||||||||||||||
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タイトル | Cryo-EM structure of the C2S2-type PSII-LHCII supercomplex from Chlamydomonas reihardtii | ||||||||||||||||||
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機能・相同性 | ![]() thylakoid light-harvesting complex / photosystem II antenna complex / nonphotochemical quenching / PSII associated light-harvesting complex II / photosystem II repair / plastoglobule / chloroplast thylakoid lumen / photosystem II assembly / photosystem II oxygen evolving complex / photosynthesis, light harvesting in photosystem I ...thylakoid light-harvesting complex / photosystem II antenna complex / nonphotochemical quenching / PSII associated light-harvesting complex II / photosystem II repair / plastoglobule / chloroplast thylakoid lumen / photosystem II assembly / photosystem II oxygen evolving complex / photosynthesis, light harvesting in photosystem I / oxygen evolving activity / photosystem II stabilization / photosystem II / photosystem II reaction center / chloroplast envelope / oxidoreductase activity, acting on diphenols and related substances as donors, oxygen as acceptor / photosystem I / photosynthetic electron transport chain / response to herbicide / photosystem II / extrinsic component of membrane / chlorophyll binding / photosynthesis, light reaction / electron transporter, transferring electrons within the cyclic electron transport pathway of photosynthesis activity / phosphate ion binding / chloroplast thylakoid membrane / response to light stimulus / photosynthetic electron transport in photosystem II / photosynthesis / electron transfer activity / protein stabilization / iron ion binding / protein domain specific binding / mRNA binding / calcium ion binding / heme binding / metal ion binding 類似検索 - 分子機能 | ||||||||||||||||||
生物種 | ![]() ![]() | ||||||||||||||||||
手法 | 単粒子再構成法 / クライオ電子顕微鏡法 / 解像度: 2.7 Å | ||||||||||||||||||
![]() | Sheng X / Li AJ / Song DF / Liu ZF | ||||||||||||||||||
資金援助 | ![]()
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![]() | ![]() タイトル: Structural insight into light harvesting for photosystem II in green algae. 著者: Xin Sheng / Akimasa Watanabe / Anjie Li / Eunchul Kim / Chihong Song / Kazuyoshi Murata / Danfeng Song / Jun Minagawa / Zhenfeng Liu / ![]() ![]() 要旨: Green algae and plants rely on light-harvesting complex II (LHCII) to collect photon energy for oxygenic photosynthesis. In Chlamydomonas reinhardtii, LHCII molecules associate with photosystem II ...Green algae and plants rely on light-harvesting complex II (LHCII) to collect photon energy for oxygenic photosynthesis. In Chlamydomonas reinhardtii, LHCII molecules associate with photosystem II (PSII) to form various supercomplexes, including the CSML type, which is the largest PSII-LHCII supercomplex in algae and plants that is presently known. Here, we report high-resolution cryo-electron microscopy (cryo-EM) maps and structural models of the CSML and CS supercomplexes from C. reinhardtii. The CS supercomplex contains an LhcbM1-LhcbM2/7-LhcbM3 heterotrimer in the strongly associated LHCII, and the LhcbM1 subunit assembles with CP43 through two interfacial galactolipid molecules. The loosely and moderately associated LHCII trimers interact closely with the minor antenna complex CP29 to form an intricate subcomplex bound to CP47 in the CSML supercomplex. A notable direct pathway is established for energy transfer from the loosely associated LHCII to the PSII reaction centre, as well as several indirect routes. Structure-based computational analysis on the excitation energy transfer within the two supercomplexes provides detailed mechanistic insights into the light-harvesting process in green algae. | ||||||||||||||||||
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構造ビューア | EMマップ: ![]() ![]() ![]() |
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ヘッダ (付随情報) | ![]() ![]() | 44.7 KB 44.7 KB | 表示 表示 | ![]() |
FSC (解像度算出) | ![]() | 13.6 KB | 表示 | ![]() |
画像 | ![]() | 37.1 KB | ||
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文書・要旨 | ![]() | 619.5 KB | 表示 | ![]() |
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文書・詳細版 | ![]() | 619.1 KB | 表示 | |
XML形式データ | ![]() | 12.9 KB | 表示 | |
CIF形式データ | ![]() | 17.3 KB | 表示 | |
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「今月の分子」の関連する項目 |
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投影像・断面図 | 画像のコントロール
画像は Spider により作成 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
ボクセルのサイズ | X=Y=Z: 1.04 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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対称性 | 空間群: 1 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
詳細 | EMDB XML:
CCP4マップ ヘッダ情報:
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試料の構成要素
+全体 : The C2S2-type PSII-LHCII supercomplex
+超分子 #1: The C2S2-type PSII-LHCII supercomplex
+分子 #1: Photosystem II protein D1
+分子 #2: Photosystem II CP47 reaction center protein
+分子 #3: Photosystem II reaction center protein Ycf12
+分子 #4: Photosystem II CP43 reaction center protein
+分子 #5: Photosystem II D2 protein
+分子 #6: Cytochrome b559 subunit alpha
+分子 #7: Cytochrome b559 subunit beta
+分子 #8: Photosystem II reaction center protein H
+分子 #9: Photosystem II reaction center protein I
+分子 #10: Photosystem II reaction center protein J
+分子 #11: Photosystem II reaction center protein K
+分子 #12: Photosystem II reaction center protein L
+分子 #13: Photosystem II reaction center protein M
+分子 #14: Oxygen-evolving enhancer protein 1, chloroplastic
+分子 #15: Oxygen-evolving enhancer protein 2, chloroplastic
+分子 #16: Oxygen-evolving enhancer protein 3, chloroplastic
+分子 #17: Photosystem II reaction center protein T
+分子 #18: Photosystem II reaction center W protein, chloroplastic
+分子 #19: 4.1 kDa photosystem II subunit
+分子 #20: Photosystem II reaction center protein Z
+分子 #21: Chlorophyll a-b binding protein, chloroplastic
+分子 #22: Chlorophyll a-b binding protein, chloroplastic
+分子 #23: Chlorophyll a-b binding protein CP29
+分子 #24: Chlorophyll a-b binding protein, chloroplastic
+分子 #25: Chlorophyll a-b binding protein, chloroplastic
+分子 #26: Predicted protein
+分子 #27: 10 kDa photosystem II polypeptide PsbR (potential)
+分子 #28: Unindentified Stromal Protein (USP)
+分子 #29: CA-MN4-O5 CLUSTER
+分子 #30: FE (II) ION
+分子 #31: CHLORIDE ION
+分子 #32: CHLOROPHYLL A
+分子 #33: PHEOPHYTIN A
+分子 #34: BETA-CAROTENE
+分子 #35: 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL
+分子 #36: 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE
+分子 #37: DIGALACTOSYL DIACYL GLYCEROL (DGDG)
+分子 #38: 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE
+分子 #39: BICARBONATE ION
+分子 #40: 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,...
+分子 #41: PROTOPORPHYRIN IX CONTAINING FE
+分子 #42: DODECYL-ALPHA-D-MALTOSIDE
+分子 #43: CHLOROPHYLL B
+分子 #44: (3R,3'R,6S)-4,5-DIDEHYDRO-5,6-DIHYDRO-BETA,BETA-CAROTENE-3,3'-DIOL
+分子 #45: (3S,5R,6S,3'S,5'R,6'S)-5,6,5',6'-DIEPOXY-5,6,5',6'- TETRAHYDRO-BE...
+分子 #46: (1R,3R)-6-{(3E,5E,7E,9E,11E,13E,15E,17E)-18-[(1S,4R,6R)-4-HYDROXY...
+分子 #47: water
-実験情報
-構造解析
手法 | クライオ電子顕微鏡法 |
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![]() | 単粒子再構成法 |
試料の集合状態 | particle |
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試料調製
濃度 | 3.6 mg/mL |
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緩衝液 | pH: 6.5 |
グリッド | モデル: Quantifoil R1.2/1.3 / 材質: COPPER / 支持フィルム - 材質: CARBON / 支持フィルム - トポロジー: HOLEY ARRAY / 前処理 - タイプ: PLASMA CLEANING |
凍結 | 凍結剤: ETHANE |
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電子顕微鏡法
顕微鏡 | FEI TITAN KRIOS |
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撮影 | フィルム・検出器のモデル: GATAN K2 SUMMIT (4k x 4k) 検出モード: SUPER-RESOLUTION / 平均電子線量: 1.875 e/Å2 |
電子線 | 加速電圧: 300 kV / 電子線源: ![]() |
電子光学系 | 照射モード: FLOOD BEAM / 撮影モード: BRIGHT FIELD / Cs: 2.7 mm |
試料ステージ | ホルダー冷却材: NITROGEN |
実験機器 | ![]() モデル: Titan Krios / 画像提供: FEI Company |
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画像解析
-原子モデル構築 1
精密化 | プロトコル: BACKBONE TRACE |
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得られたモデル | ![]() PDB-6kac: |