- EMDB-8482: Cryo-EM structure of the human TAP ATP-Binding Cassette Transporter -
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基本情報
登録情報
データベース: EMDB / ID: EMD-8482
タイトル
Cryo-EM structure of the human TAP ATP-Binding Cassette Transporter
マップデータ
Human TAP ATP-Binding Cassette Transporter, full map from Frealign alignment, scaled to molecule, B-factor corrected to -150 Angstrom^2, and moved into new padded unit cell
試料
複合体: TAP ATP-Binding Cassette Transporter
タンパク質・ペプチド: Antigen peptide transporter 1
タンパク質・ペプチド: Antigen peptide transporter 2
タンパク質・ペプチド: TAP transporter inhibitor ICP47
キーワード
membrane (生体膜) / protein (タンパク質) / ABC transporter / antigen (抗原) / presentation / TRANSPORT PROTEIN (運搬体タンパク質)
機能・相同性
機能・相同性情報
antigen processing and presentation of endogenous peptide antigen via MHC class Ib via ER pathway, TAP-dependent / ABC-type peptide transporter activity / tapasin binding / ABC-type antigen peptide transporter / ABC-type peptide antigen transporter activity / TAP complex / symbiont-mediated suppression of host adaptive immune response / TAP1 binding / peptide antigen transport / TAP2 binding ...antigen processing and presentation of endogenous peptide antigen via MHC class Ib via ER pathway, TAP-dependent / ABC-type peptide transporter activity / tapasin binding / ABC-type antigen peptide transporter / ABC-type peptide antigen transporter activity / TAP complex / symbiont-mediated suppression of host adaptive immune response / TAP1 binding / peptide antigen transport / TAP2 binding / MHC class Ib protein binding / symbiont-mediated suppression of host antigen processing and presentation / cytosol to endoplasmic reticulum transport / peptide transport / peptide transmembrane transporter activity / MHC class I protein binding / antigen processing and presentation of endogenous peptide antigen via MHC class I via ER pathway, TAP-dependent / centriolar satellite / endoplasmic reticulum-Golgi intermediate compartment membrane / Antigen Presentation: Folding, assembly and peptide loading of class I MHC / antigen processing and presentation of exogenous protein antigen via MHC class Ib, TAP-dependent / response to molecule of bacterial origin / MHC class I peptide loading complex / transmembrane transport / ADP binding / T cell mediated cytotoxicity / antigen processing and presentation of endogenous peptide antigen via MHC class I / defense response / positive regulation of T cell mediated cytotoxicity / phagocytic vesicle membrane / peptide antigen binding / protein transport / ER-Phagosome pathway / 獲得免疫系 / host cell cytoplasm / nuclear speck / host cell nucleus / endoplasmic reticulum membrane / 小胞体 / protein homodimerization activity / ATP hydrolysis activity / ATP binding / 生体膜 / metal ion binding / 細胞質 類似検索 - 分子機能
Herpesvirus ICP47 / Herpesvirus US12 family / Antigen peptide transporter 2 / ABC transporter Tap-like / Type 1 protein exporter / ABC transporter transmembrane region / ABC transporter type 1, transmembrane domain / ABC transporter integral membrane type-1 fused domain profile. / ABC transporter type 1, transmembrane domain superfamily / ABC transporter-like, conserved site ...Herpesvirus ICP47 / Herpesvirus US12 family / Antigen peptide transporter 2 / ABC transporter Tap-like / Type 1 protein exporter / ABC transporter transmembrane region / ABC transporter type 1, transmembrane domain / ABC transporter integral membrane type-1 fused domain profile. / ABC transporter type 1, transmembrane domain superfamily / ABC transporter-like, conserved site / ABC transporters family signature. / ABC transporter / ABC transporter-like, ATP-binding domain / ATP-binding cassette, ABC transporter-type domain profile. / ATPases associated with a variety of cellular activities / AAA+ ATPase domain / P-loop containing nucleoside triphosphate hydrolase 類似検索 - ドメイン・相同性
ジャーナル: Elife / 年: 2016 タイトル: Structure of the transporter associated with antigen processing trapped by herpes simplex virus. 著者: Michael L Oldham / Nikolaus Grigorieff / Jue Chen / 要旨: The transporter associated with antigen processing (TAP) is an ATP-binding cassette (ABC) transporter essential to cellular immunity against viral infection. Some persistent viruses have evolved ...The transporter associated with antigen processing (TAP) is an ATP-binding cassette (ABC) transporter essential to cellular immunity against viral infection. Some persistent viruses have evolved strategies to inhibit TAP so that they may go undetected by the immune system. The herpes simplex virus for example evades immune surveillance by blocking peptide transport with a small viral protein ICP47. In this study, we determined the structure of human TAP bound to ICP47 by electron cryo-microscopy (cryo-EM) to 4.0 Å. The structure shows that ICP47 traps TAP in an inactive conformation distinct from the normal transport cycle. The specificity and potency of ICP47 inhibition result from contacts between the tip of the helical hairpin and the apex of the transmembrane cavity. This work provides a clear molecular description of immune evasion by a persistent virus. It also establishes the molecular structure of TAP to facilitate mechanistic studies of the antigen presentation process.
EMPIAR-10816 (タイトル: Cryo-electron microscopy reconstruction of human TAP Transporter bound to Herpes Simplex Virus 1 Inhibitor ICP47 Data size: 1.5 TB Data #1: Unaligned and uncorrected multiframe movies of human TAP Transporter bound to Herpes Simplex Virus 1 Inhibitor ICP47 [micrographs - multiframe])
ダウンロード / ファイル: emd_8482.map.gz / 形式: CCP4 / 大きさ: 64 MB / タイプ: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
注釈
Human TAP ATP-Binding Cassette Transporter, full map from Frealign alignment, scaled to molecule, B-factor corrected to -150 Angstrom^2, and moved into new padded unit cell
ボクセルのサイズ
X: 0.36133 Å / Y: 0.45312 Å / Z: 0.45312 Å
密度
表面レベル
By EMDB: 0.6 / ムービー #1: 0.6
最小 - 最大
-2.1199465 - 2.8055391
平均 (標準偏差)
0.03569515 (±0.2523155)
対称性
空間群: 1
詳細
EMDB XML:
マップ形状
Axis order
Z
X
Y
Origin
0
0
0
サイズ
256
256
256
Spacing
256
256
256
セル
A: 92.50048 Å / B: 115.99872 Å / C: 115.99872 Å α=β=γ: 90.0 °
CCP4マップ ヘッダ情報:
mode
Image stored as Reals
Å/pix. X/Y/Z
0.361328125
0.45312109375
0.45312109375
M x/y/z
256
256
256
origin x/y/z
0.000
0.000
0.000
length x/y/z
92.500
115.999
115.999
α/β/γ
90.000
90.000
90.000
start NX/NY/NZ
0
0
0
NX/NY/NZ
256
256
256
MAP C/R/S
3
1
2
start NC/NR/NS
0
0
0
NC/NR/NS
256
256
256
D min/max/mean
-2.120
2.806
0.036
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添付データ
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追加マップ: Human TAP ATP-Binding Cassette Transporter, half map 2...