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- EMDB-75726: 1332E5-RBD KP3.1.1 Complex Local Refine -

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Basic information

Entry
Database: EMDB / ID: EMD-75726
Title1332E5-RBD KP3.1.1 Complex Local Refine
Map data
Sample
  • Complex: 1332E5 Fab / SARS-CoV-2 RBD KP3.1.1 Variant Complex
    • Protein or peptide: Spike glycoprotein
    • Protein or peptide: 1332E5 Heavy Chain
    • Protein or peptide: 1332E5 Light Chain
KeywordsFab / Complex / SARS-CoV-2 / VIRAL PROTEIN / VIRAL PROTEIN-IMMUNE SYSTEM complex
Biological speciesHomo sapiens (human) / Severe acute respiratory syndrome coronavirus 2
Methodsingle particle reconstruction / cryo EM / Resolution: 3.62 Å
AuthorsKizziah JL / Walter MR
Funding support United States, 1 items
OrganizationGrant numberCountry
National Institutes of Health/National Institute Of Allergy and Infectious Diseases (NIH/NIAID)R01AI161175 United States
CitationJournal: Iscience / Year: 2026
Title: Emergence of neutralizing RBD antibodies following Omicron infection with limited activity against ancestral SARS-CoV-2
Authors: Piepenbrink MS / Ma Y / Panjwani S / Blake AR / Bell AM / Kizziah JL / Mahmoud SH / Ippolito GC / Erdmann NB / Goepfert PA / Martinez-Sobrido L / Kobie JJ / Walter MR
History
DepositionFeb 26, 2026-
Header (metadata) releaseAug 26, 2026-
Map releaseAug 26, 2026-
UpdateAug 26, 2026-
Current statusAug 26, 2026Processing site: RCSB / Status: Released

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Structure visualization

Supplemental images

emd_75726.png

Downloads & links

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Map

FileDownload / File: emd_75726.map.gz / Format: CCP4 / Size: 512 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Projections & slices

Image control

Size
Brightness
Contrast
Others
AxesZ (Sec.)Y (Row.)X (Col.)
0.72 Å/pix.
x 512 pix.
= 367.104 Å		0.72 Å/pix.
x 512 pix.
= 367.104 Å		0.72 Å/pix.
x 512 pix.
= 367.104 Å

Surface

Projections

Slices (1/3)

Slices (1/2)

Slices (2/3)

Images are generated by Spider.

Voxel sizeX=Y=Z: 0.717 Å
Density

Histogram

Histogram (log scale)
Contour LevelBy AUTHOR: 0.095
Minimum - Maximum-0.64617836 - 0.9191306
Average (Standard dev.)-0.0002795058 (±0.009769891)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions512512512
Spacing512512512
CellA=B=C: 367.104 Å
α=β=γ: 90.0 °

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Supplemental data

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Half map: #2

Fileemd_75726_half_map_1.map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Half map: #1

Fileemd_75726_half_map_2.map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Sample components

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Entire : 1332E5 Fab / SARS-CoV-2 RBD KP3.1.1 Variant Complex

EntireName: 1332E5 Fab / SARS-CoV-2 RBD KP3.1.1 Variant Complex
Components
  • Complex: 1332E5 Fab / SARS-CoV-2 RBD KP3.1.1 Variant Complex
    • Protein or peptide: Spike glycoprotein
    • Protein or peptide: 1332E5 Heavy Chain
    • Protein or peptide: 1332E5 Light Chain

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Supramolecule #1: 1332E5 Fab / SARS-CoV-2 RBD KP3.1.1 Variant Complex

SupramoleculeName: 1332E5 Fab / SARS-CoV-2 RBD KP3.1.1 Variant Complex / type: complex / ID: 1 / Parent: 0 / Macromolecule list: all
Source (natural)Organism: Homo sapiens (human)

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Macromolecule #1: Spike glycoprotein

MacromoleculeName: Spike glycoprotein / type: protein_or_peptide / ID: 1 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Severe acute respiratory syndrome coronavirus 2
Molecular weightTheoretical: 139.754953 KDa
Recombinant expressionOrganism: Homo sapiens (human)
SequenceString: MGWSCIILFL VATATGVHSA YTNFTRGVYY PDKVFRSSVL HLTQDLFLPF FSNVTWFHAI SGTNGTKRFD NPVLPFNDGV YFASTEKSN IIRGWIFGTT LDSKTQSLLI VNNATNVFIK VCEFQFCNDP FLDVYHKNNK SWMESESGVY SSANNCTFEY V SQPFLMDL ...String:
MGWSCIILFL VATATGVHSA YTNFTRGVYY PDKVFRSSVL HLTQDLFLPF FSNVTWFHAI SGTNGTKRFD NPVLPFNDGV YFASTEKSN IIRGWIFGTT LDSKTQSLLI VNNATNVFIK VCEFQFCNDP FLDVYHKNNK SWMESESGVY SSANNCTFEY V SQPFLMDL EGKQGNFKNL REFVFKNIDG YFKIYSKHTP IIGRDFPQGF SALEPLVDLP IGINITRFQT LLALNRSYLT PG DSSSGWT AGAADYYVGY LQPRTFLLKY NENGTITDAV DCALDPLSET KCTLKSFTVE KGIYQTSNFR VQPTESIVRF PNV TNLCPF HEVFNATRFA SVYAWNRTRI SNCVADYSVL YNFAPFFAFK CYGVSPTKLN DLCFTNVYAD SFVIKGNEVS QIAP GQTGN IADYNYKLPD DFTGCVIAWN SNKLDSKHSG NYDYWYRSLR KSKLKPFERD ISTEIYQAGN KPCKGKGPNC YFPLE SYGF RPTYGVGHQP YRVVVLSFEL LHAPATVCGP KKSTNLVKNK CVNFNFNGLT GTGVLTKSNK KFLPFQQFGR DIVDTT DAV RDPQTLEILD ITPCSFGGVS VITPGTNTSN QVAVLYQGVN CTEVSVAIHA DQLTPTWRVY STGSNVFQTR AGCLIGA EY VNNSYECDIP IGAGICASYQ TQTKSRGSAG SVASQSIIAY TMSLGAENSV AYSNNSIAIP TNFTISVTTE ILPVSMTK T SVDCTMYICG DSTECSNLLL QYGSFCTQLK RALTGIAVEQ DKNTQEVFAQ VKQIYKTPPI KYFGGFNFSQ ILPDPSKPS KRSPIEDLLF NKVTLADAGF IKQYGDCLGD IAARDLICAQ KFNGLTVLPP LLTDEMIAQY TSALLAGTIT SGWTFGAGPA LQIPFPMQM AYRFNGIGVT QNVLYENQKL IANQFNSAIG KIQDSLFSTP SALGKLQDVV NHNAQALNTL VKQLSSKFGA I SSVLNDIL SRLDPPEAEV QIDRLITGRL QSLQTYVTQQ LIRAAEIRAS ANLAATKMSE CVLGQSKRVD FCGKGYHLMS FP QSAPHGV VFLHVTYVPA QEKNFTTAPA ICHDGKAHFP REGVFVSNGT HWFLTQRNFY EPQIITTDNT FVSGNCDVVI GIV NNTVYD PLQLELDSFK EELDKYFKNH TSPDVDLGDI SGINASVVNI QKEIDRLNEV AKNLNESLID LQELGKYEQY IASS GYIPE APRDGQAYVR KDGEWVLLST FLEGTKHHHH HHHHGGSGGL NDIFEAQKIE WHE

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Macromolecule #2: 1332E5 Heavy Chain

MacromoleculeName: 1332E5 Heavy Chain / type: protein_or_peptide / ID: 2 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 25.416613 KDa
Recombinant expressionOrganism: Homo sapiens (human)
SequenceString: MGWSCIILFL VATATGVHSQ VQLQQSGPGL VKPSETLSLT CTVSGGSISS YYWSWIRQPP GKGLEWIGYI YYSGLTNYNP SLKSRITLS VDTSKNQFSL KLNSVTAADT AVYYCATYVR LGGFWSGEAD AFDIWGPGTM VTVSSASTKG PSVFPLAPSS K STSGGTAA ...String:
MGWSCIILFL VATATGVHSQ VQLQQSGPGL VKPSETLSLT CTVSGGSISS YYWSWIRQPP GKGLEWIGYI YYSGLTNYNP SLKSRITLS VDTSKNQFSL KLNSVTAADT AVYYCATYVR LGGFWSGEAD AFDIWGPGTM VTVSSASTKG PSVFPLAPSS K STSGGTAA LGCLVKDYFP EPVTVSWNSG ALTSGVHTFP AVLQSSGLYS LSSVVTVPSS SLGTQTYICN VNHKPSNTKV DK

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Macromolecule #3: 1332E5 Light Chain

MacromoleculeName: 1332E5 Light Chain / type: protein_or_peptide / ID: 3 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 24.860705 KDa
Recombinant expressionOrganism: Homo sapiens (human)
SequenceString: MGWSCIILFL VATATGVHSQ SVLTQPASVS GSPGQSITIS CTGTSSDVGN YHLVSWYQQH PGKAPKLIIY EGSKRPSGVS NRFSGSKSG NTASLTISGL QAEDEADYYC CLYADSTTYV FGIGTKVTVL GQPKAAPSVT LFPPSSEELQ ANKATLVCLI S DFYPGAVT ...String:
MGWSCIILFL VATATGVHSQ SVLTQPASVS GSPGQSITIS CTGTSSDVGN YHLVSWYQQH PGKAPKLIIY EGSKRPSGVS NRFSGSKSG NTASLTISGL QAEDEADYYC CLYADSTTYV FGIGTKVTVL GQPKAAPSVT LFPPSSEELQ ANKATLVCLI S DFYPGAVT VAWKADSSPV KAGVETTTPS KQSNNKYAAS SYLSLTPEQW KSHRSYSCQV THEGSTVEKT VAPTECS

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

Concentration0.9 mg/mL
BufferpH: 7.2 / Details: 1x Phosphate buffered saline
GridModel: Quantifoil Active R2/1 / Material: COPPER / Mesh: 200 / Support film - Material: CARBON / Support film - topology: HOLEY ARRAY / Pretreatment - Type: GLOW DISCHARGE / Pretreatment - Time: 25 sec. / Pretreatment - Atmosphere: AIR / Pretreatment - Pressure: 0.039 kPa
VitrificationCryogen name: ETHANE / Chamber humidity: 100 % / Chamber temperature: 277 K / Instrument: FEI VITROBOT MARK IV

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Electron microscopy

MicroscopeTFS GLACIOS
TemperatureMin: 91.0 K
Image recordingFilm or detector model: FEI FALCON IV (4k x 4k) / Digitization - Dimensions - Width: 4096 pixel / Digitization - Dimensions - Height: 4096 pixel / Number grids imaged: 8 / Average electron dose: 50.0 e/Å2
Electron beamAcceleration voltage: 200 kV / Electron source: FIELD EMISSION GUN
Electron opticsC2 aperture diameter: 20.0 µm / Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Cs: 2.7 mm / Nominal defocus max: 1.75 µm / Nominal defocus min: 1.0 µm / Nominal magnification: 190000
Sample stageSpecimen holder model: OTHER / Cooling holder cryogen: NITROGEN

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Image processing

CTF correctionSoftware - Name: cryoSPARC (ver. 4.5.3) / Type: PHASE FLIPPING AND AMPLITUDE CORRECTION
Startup modelType of model: PDB ENTRY
PDB model - PDB ID:

9eli

Final reconstructionResolution.type: BY AUTHOR / Resolution: 3.62 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: cryoSPARC (ver. 4.5.3) / Number images used: 112091
Initial angle assignmentType: MAXIMUM LIKELIHOOD / Software - Name: cryoSPARC (ver. 4.5.3)
Final angle assignmentType: MAXIMUM LIKELIHOOD
Final 3D classificationSoftware - Name: cryoSPARC (ver. v4.5.3)

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Atomic model buiding 1

RefinementSpace: REAL
Output model

PDB-11it:
1332E5-RBD KP3.1.1 Complex Local Refine

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