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- EMDB-75646: C12 Portal Assembly of Bacteriophage Goslar -

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Basic information

Entry
Database: EMDB / ID: EMD-75646
TitleC12 Portal Assembly of Bacteriophage Goslar
Map data
Sample
  • Virus: Goslarvirus
    • Protein or peptide: Portal Protein gp36
    • Protein or peptide: Portal Assembly Protein gp233
    • Protein or peptide: Tail Adaptor Protein gp50 NTD
KeywordsPortal Assembly / Phage / VIRAL PROTEIN
Function / homology: / Family of unknown function (DUF7484) / : / : / Putative phage head-tail joining protein / Phage head to tail associated domain / Uncharacterized protein / Virion structural protein / Virion structural protein
Function and homology information
Biological speciesGoslarvirus
Methodsingle particle reconstruction / cryo EM / Resolution: 3.82 Å
AuthorsBasu D / Gu Y / Corbett KD
Funding support United States, 1 items
OrganizationGrant numberCountry
Howard Hughes Medical Institute (HHMI)Emerging Pathogens Initiative United States
CitationJournal: To Be Published
Title: C12 Portal Assembly of Bacteriophage Goslar
Authors: Basu D
History
DepositionFeb 18, 2026-
Header (metadata) releaseAug 19, 2026-
Map releaseAug 19, 2026-
UpdateAug 19, 2026-
Current statusAug 19, 2026Processing site: RCSB / Status: Released

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Structure visualization

Supplemental images

emd_75646.png

Downloads & links

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Map

FileDownload / File: emd_75646.map.gz / Format: CCP4 / Size: 1000 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Projections & slices

Image control

Size
Brightness
Contrast
Others
AxesZ (Sec.)Y (Row.)X (Col.)
1.4 Å/pix.
x 640 pix.
= 896. Å		1.4 Å/pix.
x 640 pix.
= 896. Å		1.4 Å/pix.
x 640 pix.
= 896. Å

Surface

Projections

Slices (1/3)

Slices (1/2)

Slices (2/3)

Images are generated by Spider.

Voxel sizeX=Y=Z: 1.4 Å
Density

Histogram

Histogram (log scale)
Contour LevelBy AUTHOR: 0.2
Minimum - Maximum-0.37600747 - 0.67376274
Average (Standard dev.)-0.0006842156 (±0.03221953)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions640640640
Spacing640640640
CellA=B=C: 896.0 Å
α=β=γ: 90.0 °

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Supplemental data

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Half map: #2

Fileemd_75646_half_map_1.map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Half map: #1

Fileemd_75646_half_map_2.map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Sample components

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Entire : Goslarvirus

EntireName: Goslarvirus
Components
  • Virus: Goslarvirus
    • Protein or peptide: Portal Protein gp36
    • Protein or peptide: Portal Assembly Protein gp233
    • Protein or peptide: Tail Adaptor Protein gp50 NTD

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Supramolecule #1: Goslarvirus

SupramoleculeName: Goslarvirus / type: virus / ID: 1 / Parent: 0 / Macromolecule list: all / NCBI-ID: 2733105 / Sci species name: Goslarvirus / Virus type: VIRION / Virus isolate: STRAIN / Virus enveloped: No / Virus empty: No
Host (natural)Organism: Escherichia coli (E. coli)

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Macromolecule #1: Portal Protein gp36

MacromoleculeName: Portal Protein gp36 / type: protein_or_peptide / ID: 1 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Goslarvirus
Molecular weightTheoretical: 108.728914 KDa
SequenceString: MNDINKGRVA LERQMSNNDV AKALMNKFVA PAPQSRLYNT RDAEGANGRF GLVVPDQMTM NTVTRATATN IEDNKNILEL FPELKRVMQ IYVAALTNPN DLKTVEMFID SEDFPGRVAE IVQPMIDTID THFKNIYKIK TLIPRIIEDV FFIKGSYNFV V LPESTLDR ...String:
MNDINKGRVA LERQMSNNDV AKALMNKFVA PAPQSRLYNT RDAEGANGRF GLVVPDQMTM NTVTRATATN IEDNKNILEL FPELKRVMQ IYVAALTNPN DLKTVEMFID SEDFPGRVAE IVQPMIDTID THFKNIYKIK TLIPRIIEDV FFIKGSYNFV V LPESTLDR AINGNGQLKL EEYGFKDNHF ASWGLLGNDS RKRHENQLPA TVSMEDYFSG VNETIPYDPV VRIDSIKFTN TL LRDKDGK FIEAKIDIPD MVTVVDNPDI LRMPILHQRR AATAVQRALR ANTLGGSGLV TLSGDPTLTA NRRELTQEQV NEI WNKLNI QRHTKVSPVY ALATPDTLDR QSVGHPGVMH VPPEALEPVH APGDPENHIG YLVQLDEFGY PVRLVEDAAY YKQL EGRLS KITADIAGTS SNGMASEMIG AAKNMIQGTS CNQVDMSAFV SSYESLMQRY IVERIVNGYL GSAVEIGATS SFYLS MLAR SLAHKQTRVL FIPREMVTYI AYEYNKYGIG RSLLESTKMI SSQRAMLMVQ ELMAKIKSSL NYTTLSVMLE DTDRNP VDT VEQALHSFAR VYQSGFPLGE TNPMDIINAY ERSSIRLKVE GNNPRFPKLN TDVSVEQRNY QSPDNELMDR YRRDHFM GL GVSPEIIDTS YQSEFAASVI TANAMQEKQF TVCREVITNG LSDHIKKYTA YSPVLLTSLR NTVRANMSKL SQAQLKQL F GSDFENGVIR NVNAYQEIDD QSATMDLDYT KPEVQEVIAD AYVSMFLNSL RVRLPDSNAR RLEDQVREYN QYKEAITAQ LDDYLTPDYV MGLGGVDETT LQMIRACIIG TAMRTWAKEN NFNQELADLF SLDDKKNAVQ DWKRMSMEHI EVMGKCMGDF ALYIQNLTK KIEEKMGAQA QDGSVSSDGS WSSDSSSDTG SSDDFGGGDD FGDTDFDTET MEETSTEETT TSEETTTDTT S EDDTSGGV V

UniProtKB: Virion structural protein

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Macromolecule #2: Portal Assembly Protein gp233

MacromoleculeName: Portal Assembly Protein gp233 / type: protein_or_peptide / ID: 2 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Goslarvirus
Molecular weightTheoretical: 31.988924 KDa
SequenceString: MGAIDYSVDR ICRTIPADIL QYCYIDRYDW RNRYPRSVED IITDWVIRKR VIGDIDIYGG LQTVIPLDDA GVTIEEVTIN CYVFHIPLE ATQGRYITSA LSIISGSVNN YGINYNGQWG AFNQTQFQRQ LCGWNPASNT LQQGMNAFGP MNVLSTANVD I IAPNTIMV ...String:
MGAIDYSVDR ICRTIPADIL QYCYIDRYDW RNRYPRSVED IITDWVIRKR VIGDIDIYGG LQTVIPLDDA GVTIEEVTIN CYVFHIPLE ATQGRYITSA LSIISGSVNN YGINYNGQWG AFNQTQFQRQ LCGWNPASNT LQQGMNAFGP MNVLSTANVD I IAPNTIMV NNVTPVQFPL SLRCFLAHDP ELTNLSMEVW NDFYKLCLYA TQSDIYNRTV IRLDRGVTAQ GQEVGAFKEY ID RFADAEN TYQEFLNETW AGVAAWNDPQ TKRRAIRLQT GYF

UniProtKB: Uncharacterized protein

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Macromolecule #3: Tail Adaptor Protein gp50 NTD

MacromoleculeName: Tail Adaptor Protein gp50 NTD / type: protein_or_peptide / ID: 3 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Goslarvirus
Molecular weightTheoretical: 49.920422 KDa
SequenceString: MALAKKPAKP NINPQQPAKA RDDVYRSAVV DEQYNSQIGL VNYASGYSVE CDYYEQILGR DDEQSSFQAN RDIAEQQYRR IRHYPIKLM SSLTPSYDNG TARFQSEGTC ILPAVSVPLL GNMLLISGLD HRIAVYTITR ITPKNISLAP AYECDISFQR I LDEKQLIE ...String:
MALAKKPAKP NINPQQPAKA RDDVYRSAVV DEQYNSQIGL VNYASGYSVE CDYYEQILGR DDEQSSFQAN RDIAEQQYRR IRHYPIKLM SSLTPSYDNG TARFQSEGTC ILPAVSVPLL GNMLLISGLD HRIAVYTITR ITPKNISLAP AYECDISFQR I LDEKQLIE LEAKIVEDTL YDQDQLKHGK HPVMATEKYN RKYDYLRRAL GLCHAYYHEF YSRELNTLLV PGQDAATYDP WC VNAFTKL WSSLEGKPSW NFGRLNVDDN QEANAISVFE WVMDGRREDE ARLTVKFTLL STKRFYQYPA AGGIRFSGAR FCM WPATTS LGEDVGKELA VPENTPPTNP LVTNLTSMEY DGVPLFPSVA DDPYYVFTEA FYRNQAPMTI IESMVRDMVD EKAI SHPAL KQMVDAIPLL SPLDRYYFTP FVIMLLRAGA IEP

UniProtKB: Virion structural protein

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

BufferpH: 7.5
GridModel: Quantifoil / Support film - Material: CARBON / Support film - topology: LACEY / Support film - Film thickness: 2
VitrificationCryogen name: ETHANE / Chamber humidity: 95 % / Chamber temperature: 277 K / Instrument: FEI VITROBOT MARK II

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Electron microscopy

MicroscopeTFS KRIOS
Image recordingFilm or detector model: GATAN K3 BIOCONTINUUM (6k x 4k) / Average electron dose: 50.0 e/Å2
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 2.0 µm / Nominal defocus min: 1.2 µm
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

CTF correctionType: PHASE FLIPPING AND AMPLITUDE CORRECTION
Startup modelType of model: INSILICO MODEL
Final reconstructionResolution.type: BY AUTHOR / Resolution: 3.82 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: cryoSPARC / Number images used: 64344
Initial angle assignmentType: MAXIMUM LIKELIHOOD
Final angle assignmentType: MAXIMUM LIKELIHOOD
FSC plot (resolution estimation)

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Atomic model buiding 1

Initial modelChain - Source name: AlphaFold / Chain - Initial model type: in silico model
Output model

PDB-11ed:
C12 Portal Assembly of Bacteriophage Goslar

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