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- EMDB-75645: Tail Tube of Bacteriophage Goslar -

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Open data


ID or keywords:

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Basic information

Entry
Database: EMDB / ID: EMD-75645
TitleTail Tube of Bacteriophage Goslar
Map data
Sample
  • Virus: Goslarvirus
    • Protein or peptide: Tail Tube of Bacteriophage Goslar
KeywordsTail tube / Phage / VIRAL PROTEIN
Function / homology: / Phage tail tube protein / Uncharacterized protein
Function and homology information
Biological speciesGoslarvirus
Methodsingle particle reconstruction / cryo EM / Resolution: 3.22 Å
AuthorsBasu D / Gu Y / Corbett KD
Funding support United States, 1 items
OrganizationGrant numberCountry
Howard Hughes Medical Institute (HHMI)Emerging Pathogens Initiative United States
CitationJournal: To Be Published
Title: Tail Tube of Bacteriophage Goslar
Authors: Basu D
History
DepositionFeb 18, 2026-
Header (metadata) releaseAug 19, 2026-
Map releaseAug 19, 2026-
UpdateAug 19, 2026-
Current statusAug 19, 2026Processing site: RCSB / Status: Released

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Structure visualization

Supplemental images

emd_75645.png

Downloads & links

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Map

FileDownload / File: emd_75645.map.gz / Format: CCP4 / Size: 282.6 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Projections & slices

Image control

Size
Brightness
Contrast
Others
AxesZ (Sec.)Y (Row.)X (Col.)
1.4 Å/pix.
x 420 pix.
= 588. Å		1.4 Å/pix.
x 420 pix.
= 588. Å		1.4 Å/pix.
x 420 pix.
= 588. Å

Surface

Projections

Slices (1/3)

Slices (1/2)

Slices (2/3)

Images are generated by Spider.

Voxel sizeX=Y=Z: 1.4 Å
Density

Histogram

Histogram (log scale)
Contour LevelBy AUTHOR: 0.358
Minimum - Maximum-0.37192178 - 1.2188098
Average (Standard dev.)0.008450984 (±0.069507286)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions420420420
Spacing420420420
CellA=B=C: 588.0 Å
α=β=γ: 90.0 °

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Supplemental data

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Half map: #2

Fileemd_75645_half_map_1.map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Half map: #1

Fileemd_75645_half_map_2.map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Sample components

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Entire : Goslarvirus

EntireName: Goslarvirus
Components
  • Virus: Goslarvirus
    • Protein or peptide: Tail Tube of Bacteriophage Goslar

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Supramolecule #1: Goslarvirus

SupramoleculeName: Goslarvirus / type: virus / ID: 1 / Parent: 0 / Macromolecule list: all / NCBI-ID: 2733105 / Sci species name: Goslarvirus / Virus type: VIRION / Virus isolate: STRAIN / Virus enveloped: No / Virus empty: No
Host (natural)Organism: Escherichia coli (E. coli)

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Macromolecule #1: Tail Tube of Bacteriophage Goslar

MacromoleculeName: Tail Tube of Bacteriophage Goslar / type: protein_or_peptide / ID: 1 / Number of copies: 12 / Enantiomer: LEVO
Source (natural)Organism: Goslarvirus
Molecular weightTheoretical: 32.990105 KDa
SequenceString: MRPDNTLLGE QGVGALSNAA MVDIRAGAQN GYISNIGTYV ANANYIPNQM FCLLLEAPRG FNYLPNPDVQ IGYLKALVEE VAHSITGLQ RGLEVEFVSV PVSGSGEIQE EVSDVKRPRS NPTFGIYEKE GRSVSYFLEQ WITYLLMDPD AKYPMLSSIV S TGGPTDLL ...String:
MRPDNTLLGE QGVGALSNAA MVDIRAGAQN GYISNIGTYV ANANYIPNQM FCLLLEAPRG FNYLPNPDVQ IGYLKALVEE VAHSITGLQ RGLEVEFVSV PVSGSGEIQE EVSDVKRPRS NPTFGIYEKE GRSVSYFLEQ WITYLLMDPD AKYPMLSSIV S TGGPTDLL ADYRSATMLF VEPDRQHKKV VNAWLCTNMM PHGTGDWTSR RNKNDAPNVV ELSIQFTALT QTNYGVRAFA QR LLDKMNL LKVNPDFRPA YLSDIDPAVA AQKVGYTFDF DNNDTFEVPN TYQNVTPRV

UniProtKB: Uncharacterized protein

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation statehelical array

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Sample preparation

BufferpH: 7.5
GridModel: Quantifoil / Support film - Material: CARBON / Support film - topology: LACEY / Support film - Film thickness: 2
VitrificationCryogen name: ETHANE / Chamber humidity: 95 % / Chamber temperature: 277 K / Instrument: FEI VITROBOT MARK II

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Electron microscopy

MicroscopeTFS KRIOS
Image recordingFilm or detector model: GATAN K3 BIOCONTINUUM (6k x 4k) / Average electron dose: 50.0 e/Å2
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 2.0 µm / Nominal defocus min: 1.2 µm
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

CTF correctionType: PHASE FLIPPING AND AMPLITUDE CORRECTION
Startup modelType of model: INSILICO MODEL
Final reconstructionApplied symmetry - Point group: C6 (6 fold cyclic) / Resolution.type: BY AUTHOR / Resolution: 3.22 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: cryoSPARC (ver. 4.7.1) / Number images used: 1143360
Initial angle assignmentType: MAXIMUM LIKELIHOOD
Final angle assignmentType: MAXIMUM LIKELIHOOD
FSC plot (resolution estimation)

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Atomic model buiding 1

Initial modelChain - Source name: AlphaFold / Chain - Initial model type: in silico model
Output model

PDB-11ec:
Tail Tube of Bacteriophage Goslar

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