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- EMDB-75369: TASK-2 K245A at pH 6.5 -

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ID or keywords:

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Basic information

Entry
Database: EMDB / ID: EMD-75369
TitleTASK-2 K245A at pH 6.5
Map dataSharpened map
Sample
  • Complex: TASK-2 K245A at pH 6.5
    • Protein or peptide: Potassium channel TASK2
  • Ligand: POTASSIUM ION
  • Ligand: 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine
KeywordsPOTASSIUM ION CHANNEL / K2P CHANNEL / TRANSPORT PROTEIN
Function / homology
Function and homology information


Phase 4 - resting membrane potential / potassium ion export across plasma membrane / regulation of resting membrane potential / potassium ion leak channel activity / outward rectifier potassium channel activity / potassium ion import across plasma membrane / potassium channel activity / voltage-gated potassium channel activity / potassium ion transmembrane transport / protein heterodimerization activity / plasma membrane
Similarity search - Function
Two pore domain potassium channel, TASK family / Two pore domain potassium channel / Potassium channel domain / Ion channel
Similarity search - Domain/homology
Potassium channel TASK2
Similarity search - Component
Biological speciesMus musculus (house mouse)
Methodsingle particle reconstruction / cryo EM / Resolution: 3.22 Å
AuthorsDocter T / Rietmeijer R / Cook ASI / Brohawn SG
Funding support United States, 1 items
OrganizationGrant numberCountry
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)GM145869 United States
CitationJournal: To Be Published
Title: A shared site for lipid and anesthetic block of the two-pore domain K+ channel TASK-2
Authors: Docter T / Sorum B / Li B / Rietmeijer RA / Cook ASI / Kotecha A / Brohawn SG
History
DepositionJan 30, 2026-
Header (metadata) releaseOct 7, 2026-
Map releaseOct 7, 2026-
UpdateOct 7, 2026-
Current statusOct 7, 2026Processing site: RCSB / Status: Released

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Structure visualization

Supplemental images

emd_75369.png

Downloads & links

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Map

FileDownload / File: emd_75369.map.gz / Format: CCP4 / Size: 22.2 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
AnnotationSharpened map
Projections & slices

Image control

Size
Brightness
Contrast
Others
AxesZ (Sec.)Y (Row.)X (Col.)
1.12 Å/pix.
x 180 pix.
= 200.7 Å		1.12 Å/pix.
x 180 pix.
= 200.7 Å		1.12 Å/pix.
x 180 pix.
= 200.7 Å

Surface

Projections

Slices (1/3)

Slices (1/2)

Slices (2/3)

Images are generated by Spider.

Voxel sizeX=Y=Z: 1.115 Å
Density

Histogram

Histogram (log scale)
Contour LevelBy AUTHOR: 0.304
Minimum - Maximum-3.2651262 - 4.50682
Average (Standard dev.)-0.0004546053 (±0.11021008)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions180180180
Spacing180180180
CellA=B=C: 200.7 Å
α=β=γ: 90.0 °

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Supplemental data

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Additional map: Unsharpened map

Fileemd_75369_additional_1.map
AnnotationUnsharpened map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Half map: Half map A

Fileemd_75369_half_map_1.map
AnnotationHalf map A
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Half map: Half map B

Fileemd_75369_half_map_2.map
AnnotationHalf map B
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Sample components

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Entire : TASK-2 K245A at pH 6.5

EntireName: TASK-2 K245A at pH 6.5
Components
  • Complex: TASK-2 K245A at pH 6.5
    • Protein or peptide: Potassium channel TASK2
  • Ligand: POTASSIUM ION
  • Ligand: 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine

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Supramolecule #1: TASK-2 K245A at pH 6.5

SupramoleculeName: TASK-2 K245A at pH 6.5 / type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1
Source (natural)Organism: Mus musculus (house mouse)
Molecular weightTheoretical: 77 KDa

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Macromolecule #1: Potassium channel TASK2

MacromoleculeName: Potassium channel TASK2 / type: protein_or_peptide / ID: 1 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Mus musculus (house mouse)
Molecular weightTheoretical: 38.60373 KDa
Recombinant expressionOrganism: Komagataella pastoris (fungus)
SequenceString: MVDRGPLLTS AIIFYLAIGA AIFEVLEEPH WKEAKKNYYT QKLHLLKEFP CLSQEGLDKI LQVVSDAADQ GVAITGNQTF NNWNWPNAM IFAATVITTI GYGNVAPKTP AGRLFCVFYG LFGVPLCLTW ISALGKFFGG RAKRLGQFLT RRGVSLRKAQ I TCTAIFIV ...String:
MVDRGPLLTS AIIFYLAIGA AIFEVLEEPH WKEAKKNYYT QKLHLLKEFP CLSQEGLDKI LQVVSDAADQ GVAITGNQTF NNWNWPNAM IFAATVITTI GYGNVAPKTP AGRLFCVFYG LFGVPLCLTW ISALGKFFGG RAKRLGQFLT RRGVSLRKAQ I TCTAIFIV WGVLVHLVIP PFVFMVTEEW NYIEGLYYSF ITISTIGFGD FVAGVNPSAN YHALYRYFVE LWIYLGLAWL SL FVNWAVS MFVEVHKAIK KRRRRRKESF ESSPHSRKAL QMAGSTASKD VNIFSFLSKK EETYNDLIKQ IGKKAMKTSG GGE RVPGPG HGLGPQGDRS NSLEVLFQ

UniProtKB: Potassium channel TASK2

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Macromolecule #2: POTASSIUM ION

MacromoleculeName: POTASSIUM ION / type: ligand / ID: 2 / Number of copies: 3 / Formula: K
Molecular weightTheoretical: 39.098 Da

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Macromolecule #3: 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine

MacromoleculeName: 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine / type: ligand / ID: 3 / Number of copies: 16 / Formula: PEE
Molecular weightTheoretical: 744.034 Da
Chemical component information

ChemComp-PEE:
1,2-dioleoyl-sn-glycero-3-phosphoethanolamine / DOPE, phospholipid*YM

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

BufferpH: 6.5
VitrificationCryogen name: ETHANE

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Electron microscopy

MicroscopeFEI TALOS ARCTICA
Image recordingFilm or detector model: GATAN K3 BIOQUANTUM (6k x 4k) / Average electron dose: 50.0 e/Å2
Electron beamAcceleration voltage: 200 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 1.5 µm / Nominal defocus min: 0.5 µm
Experimental equipment
Model: Talos Arctica / Image courtesy: FEI Company

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Image processing

CTF correctionType: PHASE FLIPPING AND AMPLITUDE CORRECTION
Startup modelType of model: PDB ENTRY
PDB model - PDB ID:

6wm0

Final reconstructionResolution.type: BY AUTHOR / Resolution: 3.22 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: cryoSPARC / Number images used: 310671
Initial angle assignmentType: MAXIMUM LIKELIHOOD
Final angle assignmentType: MAXIMUM LIKELIHOOD

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