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- EMDB-75081: Cryo-EM structure of major outer membrane protein (PorA) with Loo... -

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Basic information

Entry
Database: EMDB / ID: EMD-75081
TitleCryo-EM structure of major outer membrane protein (PorA) with Loop4 missing
Map data
Sample
  • Complex: Major outer membrane protein
    • Protein or peptide: Major outer membrane protein
KeywordsC. jejuni / Major Outer Membrane Protein / porA / porin / MEMBRANE PROTEIN
Function / homologyCampylobacter major outer membrane / Campylobacter major outer membrane protein / porin activity / pore complex / monoatomic ion transport / cell outer membrane / cell adhesion / Major outer membrane protein
Function and homology information
Biological speciesCampylobacter jejuni (Campylobacter)
Methodsingle particle reconstruction / cryo EM / Resolution: 2.74 Å
AuthorsZhang Z / Gregor W
Funding support United States, 1 items
OrganizationGrant numberCountry
National Institutes of Health/National Institute Of Allergy and Infectious Diseases (NIH/NIAID) United States
CitationJournal: To Be Published
Title: Cryo-EM structure of major outer membrane protein (PorA) with Loop4 missing
Authors: Zhang Z / Gregor W
History
DepositionJan 13, 2026-
Header (metadata) releaseAug 12, 2026-
Map releaseAug 12, 2026-
UpdateAug 12, 2026-
Current statusAug 12, 2026Processing site: RCSB / Status: Released

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Structure visualization

Supplemental images

emd_75081.png

Downloads & links

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Map

FileDownload / File: emd_75081.map.gz / Format: CCP4 / Size: 103 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Projections & slices

Image control

Size
Brightness
Contrast
Others
AxesZ (Sec.)Y (Row.)X (Col.)
1.07 Å/pix.
x 300 pix.
= 321. Å		1.07 Å/pix.
x 300 pix.
= 321. Å		1.07 Å/pix.
x 300 pix.
= 321. Å

Surface

Projections

Slices (1/3)

Slices (1/2)

Slices (2/3)

Images are generated by Spider.

Voxel sizeX=Y=Z: 1.07 Å
Density

Histogram

Histogram (log scale)
Contour LevelBy AUTHOR: 0.1
Minimum - Maximum-0.4202715 - 0.9622508
Average (Standard dev.)-0.0005421062 (±0.021981847)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions300300300
Spacing300300300
CellA=B=C: 321.00003 Å
α=β=γ: 90.0 °

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Supplemental data

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Mask #1

Fileemd_75081_msk_1.map
Projections & Slices
AxesZYX

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Histogram

Histogram (log scale)

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Additional map: sharp map

Fileemd_75081_additional_1.map
Annotationsharp map
Projections & Slices
AxesZYX

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Histogram

Histogram (log scale)

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Half map: #2

Fileemd_75081_half_map_1.map
Projections & Slices
AxesZYX

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Histogram

Histogram (log scale)

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Half map: #1

Fileemd_75081_half_map_2.map
Projections & Slices
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Sample components

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Entire : Major outer membrane protein

EntireName: Major outer membrane protein
Components
  • Complex: Major outer membrane protein
    • Protein or peptide: Major outer membrane protein

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Supramolecule #1: Major outer membrane protein

SupramoleculeName: Major outer membrane protein / type: complex / ID: 1 / Parent: 0 / Macromolecule list: all
Source (natural)Organism: Campylobacter jejuni (Campylobacter)

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Macromolecule #1: Major outer membrane protein

MacromoleculeName: Major outer membrane protein / type: protein_or_peptide / ID: 1 / Number of copies: 3 / Enantiomer: LEVO
Source (natural)Organism: Campylobacter jejuni (Campylobacter)
Molecular weightTheoretical: 45.723184 KDa
SequenceString: MKLVKLSLVA ALAAGAFSAA NATPLEEAIK DVDVSGVLRY RYDTGNFDKN FVNNSNLNNS KQDHKYRAQV NFSAAIADNF KAFVQFDYN AADGGYGANG IKNDQKGLFV RQLYLTYTNE DVATSVIAGK QQLNLIWTDN AIDGLVGTGV KVVNNSIDGL T LAAFAVDS ...String:
MKLVKLSLVA ALAAGAFSAA NATPLEEAIK DVDVSGVLRY RYDTGNFDKN FVNNSNLNNS KQDHKYRAQV NFSAAIADNF KAFVQFDYN AADGGYGANG IKNDQKGLFV RQLYLTYTNE DVATSVIAGK QQLNLIWTDN AIDGLVGTGV KVVNNSIDGL T LAAFAVDS FMAAEQGADL LEHSNISTTS NQAPFKVDSV GNLYGAAAVG SYDLAGGQFN PQLWLAYWDQ VAFFYAVDAA YS TTIFDGI NWTLEGAYLG NSLDSELDDK THANGNLFAL KGSIEVNGWD ASLGGLYYGD KEKASTVVIE DQGNLGSLLA GEE IFYTTG SRLNGDTGRN IFGYVTGGYT FNETVRVGAD FVYGGTKTEA ANHLGGGKKL EAVARVDYKY SPKLNFSAFY SYVN LDQGV NTNESADHST VRLQALYKF

UniProtKB: Major outer membrane protein

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

BufferpH: 7.5
VitrificationCryogen name: ETHANE

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Electron microscopy

MicroscopeTFS KRIOS
Image recordingFilm or detector model: GATAN K3 BIOQUANTUM (6k x 4k) / Average electron dose: 50.0 e/Å2
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 1.5 µm / Nominal defocus min: 0.8 µm
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

CTF correctionType: PHASE FLIPPING AND AMPLITUDE CORRECTION
Startup modelType of model: INSILICO MODEL
Final reconstructionResolution.type: BY AUTHOR / Resolution: 2.74 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: cryoSPARC / Number images used: 50557
Initial angle assignmentType: ANGULAR RECONSTITUTION
Final angle assignmentType: ANGULAR RECONSTITUTION
FSC plot (resolution estimation)

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