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- EMDB-73814: Cryo-EM structure of rabbit major vault protein complex -

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Basic information

Entry
Database: EMDB / ID: EMD-73814
TitleCryo-EM structure of rabbit major vault protein complex
Map data
Sample
  • Organelle or cellular component: Major vault protein complex
    • Protein or peptide: Major vault protein
Keywordscomplex / MVP / vault cap / trafficking / STRUCTURAL PROTEIN
Function / homology
Function and homology information


protein activation cascade / ERBB signaling pathway / negative regulation of epidermal growth factor receptor signaling pathway / cell population proliferation / protein phosphatase binding / cytoskeleton / ribonucleoprotein complex / protein kinase binding / perinuclear region of cytoplasm / identical protein binding ...protein activation cascade / ERBB signaling pathway / negative regulation of epidermal growth factor receptor signaling pathway / cell population proliferation / protein phosphatase binding / cytoskeleton / ribonucleoprotein complex / protein kinase binding / perinuclear region of cytoplasm / identical protein binding / nucleus / cytosol
Similarity search - Function
Major vault protein, N-terminal / Major vault protein, shoulder domain / Major vault protein / Major vault protein repeat domain 3 / Major vault protein repeat domain 2 / Major vault protein repeat domain 4 / Major vault protein repeat domain / Major vault protein repeat domain superfamily / Major vault protein repeat domain 2 superfamily / Major Vault Protein repeat domain ...Major vault protein, N-terminal / Major vault protein, shoulder domain / Major vault protein / Major vault protein repeat domain 3 / Major vault protein repeat domain 2 / Major vault protein repeat domain 4 / Major vault protein repeat domain / Major vault protein repeat domain superfamily / Major vault protein repeat domain 2 superfamily / Major Vault Protein repeat domain / Shoulder domain / Major Vault Protein repeat domain / Major Vault Protein Repeat domain / Major Vault Protein repeat domain / MVP (vault) repeat profile. / Band 7/SPFH domain superfamily
Similarity search - Domain/homology
Biological speciesOryctolagus cuniculus (rabbit)
Methodsingle particle reconstruction / cryo EM / negative staining / Resolution: 2.4 Å
AuthorsLi H / Clarke OB
Funding support1 items
OrganizationGrant numberCountry
Not funded
CitationJournal: Sci Adv / Year: 2026
Title: The vault particle is enclosed by a 13-symmetric cap with a positively charged exterior.
Authors: Huan Li / Francesca Vallese / Oliver B Clarke /
Abstract: Vaults are some of the largest ribonucleoprotein complexes known and are highly conserved across eukaryotes, but both their function and key details of their architecture remain unclear. While high- ...Vaults are some of the largest ribonucleoprotein complexes known and are highly conserved across eukaryotes, but both their function and key details of their architecture remain unclear. While high-resolution structures of the vault shell are available, the architecture and symmetry of the cap have remained unresolved. Here, we present a 2.25-angstrom cryo-electron microscopy structure of the vault cap, revealing an unexpected 13-fold symmetric arrangement that contrasts with the 39-fold symmetry of the vault body, with each repeating module of the cap formed by an asymmetric homotrimer of adjacent subunits. The center of the cap features an unusual architecture, consisting of two concentric β barrels surrounded by an interwoven two-layer stack of α helices. The vault cap features a positively charged exterior and a negatively charged interior surface, with implications for binding partner recruitment and engineering of modified vault particles.
History
DepositionNov 11, 2025-
Header (metadata) releaseJan 21, 2026-
Map releaseJan 21, 2026-
UpdateAug 5, 2026-
Current statusAug 5, 2026Processing site: RCSB / Status: Released

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Structure visualization

Supplemental images

emd_73814.png

Downloads & links

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Map

FileDownload / File: emd_73814.map.gz / Format: CCP4 / Size: 1.3 GB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Projections & slices

Image control

Size
Brightness
Contrast
Others
AxesZ (Sec.)Y (Row.)X (Col.)
1.11 Å/pix.
x 700 pix.
= 773.5 Å		1.11 Å/pix.
x 700 pix.
= 773.5 Å		1.11 Å/pix.
x 700 pix.
= 773.5 Å

Surface

Projections

Slices (1/3)

Slices (1/2)

Slices (2/3)

Images are generated by Spider.

Voxel sizeX=Y=Z: 1.105 Å
Density

Histogram

Histogram (log scale)
Contour LevelBy AUTHOR: 0.0753
Minimum - Maximum0.0 - 0.8552658
Average (Standard dev.)0.003834127 (±0.020665374)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions700700700
Spacing700700700
CellA=B=C: 773.5 Å
α=β=γ: 90.0 °

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Supplemental data

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Sample components

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Entire : Major vault protein complex

EntireName: Major vault protein complex
Components
  • Organelle or cellular component: Major vault protein complex
    • Protein or peptide: Major vault protein

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Supramolecule #1: Major vault protein complex

SupramoleculeName: Major vault protein complex / type: organelle_or_cellular_component / ID: 1 / Parent: 0 / Macromolecule list: all
Source (natural)Organism: Oryctolagus cuniculus (rabbit)

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Macromolecule #1: Major vault protein

MacromoleculeName: Major vault protein / type: protein_or_peptide / ID: 1 / Number of copies: 78 / Enantiomer: LEVO
Source (natural)Organism: Oryctolagus cuniculus (rabbit)
Molecular weightTheoretical: 98.695156 KDa
SequenceString: MATEESIIRI PPYHYIHVLD QNSNVSRVEV GPKTYIRQDN ERILFDPVRM VTVPLRHYCV VANPVVRDAQ GSVVLDVTGQ VRLRHADME IRLTQDPFPL YPGELMEKGI TPLEVVLPNT ALHLRALLDF EDSNGEKVVA GDEWLFEGPG TYIPQKEVEV L QIIHATII ...String:
MATEESIIRI PPYHYIHVLD QNSNVSRVEV GPKTYIRQDN ERILFDPVRM VTVPLRHYCV VANPVVRDAQ GSVVLDVTGQ VRLRHADME IRLTQDPFPL YPGELMEKGI TPLEVVLPNT ALHLRALLDF EDSNGEKVVA GDEWLFEGPG TYIPQKEVEV L QIIHATII RPNEALRLRA RKEFCDRDGK QRVTGEEWLV RSVGAYLPGV FEEVLDLVGA VILTEKTALH LRARRNFQDV RG VTRRTGE EWLVTVQDTE AHVPDVHEEV LGVVPITTLG PQNYCVILDP VGPDGKNQLG QKRVVKGEKS FFLQPGESLE QGI QDVYVL SEQQGLLLRA LQPLEEGEGD ERVSHQAGDR WLIRGPLEYV PPVKVEVVEE RQAIPLDENE GIYVQDVKTG KVRA VIGST YMLTQDEILW DKELPPGVEE LLNKGHDPLA DRGEKVMAKP RQPPGPRNKT RVVSYRVPHN AAVQVYDYRE KKARV VFGP ELVSLGPEEQ FTVLSLSAGR PKRPHARRAL CLLLGPDFFT DVITIETADH ARLQLQLAYN WHFEVSDQRD PQETAK LFS VPDFVGDACK AIASRVRGAV ASVTFDDFHK NSARIIRAAV FGFETAEAKG PDGMALPRPR DRAVFPQNGL VVSSVDV QS VEPVDQRTRD ALQRSVQLAI EITTNSQEAA AKHEAQRLEQ EARGRLERQK ILDQSEAEKA RKELLELEAL SMAVESTG T AKAEAESRAE AARIEGEGSV LQAKLKAQAL AIETEAELQR VQKVRELELV YARAQLELEV SKAQQLAEVE AKKFKQMTE ALGPSTIKDL AVAGPEMQVK LLQSLGLKST LITDGSTPVN LFNTAFGLLG LGADGQPLGR MTAGGPRLQE AASPQSPSAP LAPGSTHIL P

UniProtKB: Major vault protein

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Experimental details

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Structure determination

Methodnegative staining, cryo EM
Processingsingle particle reconstruction
Aggregation statetissue

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Sample preparation

BufferpH: 6.8
StainingType: NEGATIVE / Material: Uranyl Acetate
VitrificationCryogen name: ETHANE

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Electron microscopy

MicroscopeTFS KRIOS
Image recordingFilm or detector model: GATAN K3 (6k x 4k) / Average electron dose: 34.4 e/Å2
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: FLOOD BEAM / Imaging mode: DIFFRACTION / Nominal defocus max: 1.5 µm / Nominal defocus min: 0.5 µm
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

CTF correctionType: PHASE FLIPPING AND AMPLITUDE CORRECTION
Startup modelType of model: PDB ENTRY
PDB model - PDB ID:

7pkr

Final reconstructionResolution.type: BY AUTHOR / Resolution: 2.4 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: cryoSPARC / Number images used: 159668
Initial angle assignmentType: MAXIMUM LIKELIHOOD
Final angle assignmentType: MAXIMUM LIKELIHOOD

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