EMDB-71580: Porcine ATP synthase with inhibitory protein IF1, DP-state

Basic information

Entry

Database: EMDB / ID: EMD-71580

• Title: Porcine ATP synthase with inhibitory protein IF1, DP-state
• Map data: ATP synthase with inhibitory protein IF1, DP-state

Sample

• Complex: Mitochondrial ATP synthase
  • Protein or peptide: x 16 types
• Ligand: x 3 types

• Keywords: ATP synthesis / Complex V / mitochondria / oxidative-phosphorylation / MEMBRANE PROTEIN / TRANSLOCASE

Function / homology

Function:

Formation of ATP by chemiosmotic coupling / Cristae formation / mitochondrial proton-transporting ATP synthase complex binding / : / : / mitochondrial depolarization / negative regulation of mitochondrial ATP synthesis coupled proton transport / angiostatin binding / ATP biosynthetic process / positive regulation of type 2 mitophagy / ATPase inhibitor activity / Mitochondrial translation termination / Mitochondrial protein degradation / proton channel activity / negative regulation of hydrolase activity / negative regulation of endothelial cell proliferation / heme biosynthetic process / proton transmembrane transporter activity / proton motive force-driven ATP synthesis / proton-transporting two-sector ATPase complex, proton-transporting domain / proton motive force-driven mitochondrial ATP synthesis / negative regulation of cardiac muscle cell apoptotic process / response to ischemia / H+-transporting two-sector ATPase / proton-transporting ATP synthase complex / proton-transporting ATP synthase activity, rotational mechanism / proton transmembrane transport / erythrocyte differentiation / ADP binding / mitochondrial membrane / ATPase binding / calmodulin binding / mitochondrial inner membrane / lipid binding / cell surface / protein-containing complex / mitochondrion / ATP binding / metal ion binding / identical protein binding / plasma membrane / cytoplasm

Domain/homology:

ATP synthase, F0 complex, subunit G, mitochondrial / ATP synthase, F0 complex, subunit E, mitochondrial / Mitochondrial ATP synthase subunit g, animal / Mitochondrial ATP synthase g subunit / ATP synthase E chain / ATP synthase protein 8, metazoa / Mitochondrial F1-F0 ATP synthase subunit F, predicted / ATP synthase protein 8, mammals / ATP synthase protein 8 / Mitochondrial F1F0-ATP synthase, subunit f / Mitochondrial ATPase inhibitor / Mitochondrial ATPase inhibitor, IATP / ATP synthase-coupling factor 6, mitochondrial / ATP synthase-coupling factor 6 superfamily, mitochondrial / Mitochondrial ATP synthase coupling factor 6 / : / Metazoan delta subunit of F1F0-ATP synthase, C-terminal domain / ATP synthase, F0 complex, subunit B/MI25 / ATP synthase, F0 complex, subunit B / Mitochondrial ATP synthase B chain precursor (ATP-synt_B) / ATP synthase, F0 complex, subunit D, mitochondrial / ATP synthase D chain, mitochondrial (ATP5H) / ATP synthase delta/epsilon subunit, C-terminal domain superfamily / ATP synthase, F0 complex, subunit D superfamily, mitochondrial / ATP synthase, F0 complex, subunit A, bacterial/mitochondria / ATP synthase, F1 complex, epsilon subunit, mitochondrial / ATP synthase, F1 complex, epsilon subunit superfamily, mitochondrial / Mitochondrial ATP synthase epsilon chain / ATPase, OSCP/delta subunit, conserved site / ATP synthase delta (OSCP) subunit signature. / F1F0 ATP synthase OSCP/delta subunit, N-terminal domain superfamily / ATP synthase, F0 complex, subunit A / ATP synthase, F0 complex, subunit A, active site / ATP synthase, F0 complex, subunit A superfamily / ATP synthase A chain / ATP synthase a subunit signature. / ATPase, OSCP/delta subunit / ATP synthase delta (OSCP) subunit / ATP synthase, F1 complex, delta/epsilon subunit / ATP synthase, F1 complex, delta/epsilon subunit, N-terminal / F0F1 ATP synthase delta/epsilon subunit, N-terminal / ATP synthase, Delta/Epsilon chain, beta-sandwich domain / ATP synthase, F0 complex, subunit C / F1F0 ATP synthase subunit C superfamily / ATP synthase, F0 complex, subunit C, DCCD-binding site / ATP synthase c subunit signature. / ATP synthase, F1 complex, gamma subunit conserved site / ATP synthase gamma subunit signature. / ATP synthase, F1 complex, beta subunit / ATP synthase, alpha subunit, C-terminal domain superfamily / : / ATP synthase, F1 complex, alpha subunit nucleotide-binding domain / ATP synthase, F1 complex, gamma subunit / ATP synthase, F1 complex, gamma subunit superfamily / ATP synthase / ATP synthase, alpha subunit, C-terminal / ATP synthase, F1 complex, alpha subunit / ATP synthase alpha/beta chain, C terminal domain / V-ATPase proteolipid subunit C-like domain / F/V-ATP synthase subunit C superfamily / ATP synthase subunit C / : / ATPase, F1/V1 complex, beta/alpha subunit, C-terminal / C-terminal domain of V and A type ATP synthase / ATPase, F1/V1/A1 complex, alpha/beta subunit, N-terminal domain superfamily / ATP synthase subunit alpha, N-terminal domain-like superfamily / ATPase, F1/V1/A1 complex, alpha/beta subunit, N-terminal domain / ATP synthase alpha/beta family, beta-barrel domain / ATPase, alpha/beta subunit, nucleotide-binding domain, active site / ATP synthase alpha and beta subunits signature. / ATPase, F1/V1/A1 complex, alpha/beta subunit, nucleotide-binding domain / ATP synthase alpha/beta family, nucleotide-binding domain / ATPases associated with a variety of cellular activities / AAA+ ATPase domain / P-loop containing nucleoside triphosphate hydrolase

Component:

ATP synthase subunit beta / ATP synthase subunit b / ATP synthase F(1) complex subunit delta, mitochondrial / ATP synthase F1 subunit epsilon / ATP synthase subunit gamma / ATP synthase peripheral stalk subunit d, mitochondrial / ATP synthase F(0) complex subunit g, mitochondrial / ATP synthase peripheral stalk subunit F6, mitochondrial / ATP synthase F(1) complex subunit alpha, mitochondrial / ATPase inhibitor, mitochondrial / ATP synthase peripheral stalk subunit OSCP, mitochondrial / ATP synthase F(0) complex subunit 8 / ATP synthase F(0) complex subunit a / ATP synthase lipid-binding protein / ATP synthase F(0) complex subunit f, mitochondrial / ATP synthase F(0) complex subunit e, mitochondrial

• Biological species: Sus scrofa (pig)
• Method: single particle reconstruction / cryo EM / Resolution: 2.69 Å
• Authors: Mnatsakanyan N / Mello JFR / Palles C

Funding support

United States, 2 items

Organization	Grant number	Country
National Institutes of Health/National Institute on Aging (NIH/NIA)	RF1AG072484	United States
National Institutes of Health/National Institute of Neurological Disorders and Stroke (NIH/NINDS)	R21NS137275	United States

• Citation: Journal: To Be Published; Title: Porcine ATP synthase with inhibitory protein IF1, DP-state; Authors: Mnatsakanyan N / Mello JFR / Palles C

History

Deposition	Jul 2, 2025	-
Header (metadata) release	Aug 19, 2026	-
Map release	Aug 19, 2026	-
Update	Aug 19, 2026	-
Current status	Aug 19, 2026	Processing site: RCSB / Status: Released

Map

• File: Download / File: emd_71580.map.gz / Format: CCP4 / Size: 512 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
• Annotation: ATP synthase with inhibitory protein IF1, DP-state
• Voxel size: X=Y=Z: 1.068 Å

Density

Contour Level	By EMDB: 5.1
Minimum - Maximum	-24.262153999999999 - 51.984454999999997
Average (Standard dev.)	0.012466257 (±1.0761626)

• Symmetry: Space group: 1

Details

EMDB XML:

Map geometry	Axis order Z Y X Origin 0 0 0 Dimensions 512 512 512 Spacing 512 512 512
Cell	A=B=C: 546.816 Å α=β=γ: 90.0 °

Supplemental data

Half map: ATP synthase with inhibitory protein IF1, DP-state

• File: emd_71580_half_map_1.map
• Annotation: ATP synthase with inhibitory protein IF1, DP-state

Half map: ATP synthase with inhibitory protein IF1, DP-state

• File: emd_71580_half_map_2.map
• Annotation: ATP synthase with inhibitory protein IF1, DP-state

Sample components

Entire : Mitochondrial ATP synthase

• Entire: Name: Mitochondrial ATP synthase

Components

• Complex: Mitochondrial ATP synthase
  • Protein or peptide: ATP synthase lipid-binding protein
  • Protein or peptide: ATP synthase F(1) complex subunit alpha, mitochondrial
  • Protein or peptide: ATP synthase subunit beta
  • Protein or peptide: ATP synthase subunit gamma
  • Protein or peptide: ATP synthase F(1) complex subunit delta, mitochondrial
  • Protein or peptide: ATP synthase F1 subunit epsilon
  • Protein or peptide: ATPase inhibitor, mitochondrial
  • Protein or peptide: ATP synthase subunit b
  • Protein or peptide: ATP synthase peripheral stalk subunit F6, mitochondrial
  • Protein or peptide: ATP synthase peripheral stalk subunit d, mitochondrial
  • Protein or peptide: ATP synthase subunit a
  • Protein or peptide: ATP synthase peripheral stalk subunit OSCP, mitochondrial
  • Protein or peptide: ATP synthase F(0) complex subunit 8
  • Protein or peptide: ATP synthase F(0) complex subunit f, mitochondrial
  • Protein or peptide: ATP synthase F(0) complex subunit g, mitochondrial
  • Protein or peptide: ATP synthase F(0) complex subunit e, mitochondrial
• Ligand: ADENOSINE-5'-TRIPHOSPHATE
• Ligand: MAGNESIUM ION
• Ligand: ADENOSINE-5'-DIPHOSPHATE

Supramolecule #1: Mitochondrial ATP synthase

• Supramolecule: Name: Mitochondrial ATP synthase / type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#16
• Source (natural): Organism: Sus scrofa (pig)

Macromolecule #1: ATP synthase lipid-binding protein

• Macromolecule: Name: ATP synthase lipid-binding protein / type: protein_or_peptide / ID: 1 / Number of copies: 8 / Enantiomer: LEVO
• Source (natural): Organism: Sus scrofa (pig)
• Molecular weight: Theoretical: 7.426718 KDa

Sequence

String:

DIDTAAKFIG AGAATVGVAG SGAGIGTVFG SMIIGYARNP SLKQQLFSYA ILGFALSEAM GLFCLMVAFL ILF

UniProtKB: ATP synthase lipid-binding protein

Macromolecule #2: ATP synthase F(1) complex subunit alpha, mitochondrial

• Macromolecule: Name: ATP synthase F(1) complex subunit alpha, mitochondrial; type: protein_or_peptide / ID: 2 / Number of copies: 3 / Enantiomer: LEVO
• Source (natural): Organism: Sus scrofa (pig)
• Molecular weight: Theoretical: 55.100027 KDa

Sequence

String:

KTGTAEVSSI LEERILGADT SVDLEETGRV LSIGDGIARV HGLRNVQAEE MVEFSSGLKG MSLNLEPDNV GVVVFGNDKL IKEGDIVKR TGAIVDVPVG EELLGRVVDA LGNAIDGKGP IGSKTRRRVG LKAPGIIPRI SVREPMQTGI KAVDSLVPIG R GQRELIIG DRQTGKTSIA IDTIINQKRF NDGTDEKKKL YCIYVAIGQK RSTVAQLVKR LTDADAMKYT IVVSATASDA AP LQYLAPY SGCSMGEYFR DNGKHALIIY DDLSKQAVAY RQMSLLLRRP PGREAYPGDV FYLHSRLLER AAKMNDAFGG GSL TALPVI ETQAGDVSAY IPTNVISITD GQIFLETELF YKGIRPAINV GLSVSRVGSA AQTRAMKQVA GTMKLELAQY REVA AFAQF GSDLDAATQQ LLSRGVRLTE LLKQGQYAPM AIEEQVAVIY AGVRGYLDKL EPSKITKFEN AFLSHVISQH QALLG KIRA DGKISEETDA KLKEIVTNFL AGFE

UniProtKB: ATP synthase F(1) complex subunit alpha, mitochondrial

Macromolecule #3: ATP synthase subunit beta

• Macromolecule: Name: ATP synthase subunit beta / type: protein_or_peptide / ID: 3 / Number of copies: 3 / Enantiomer: LEVO / EC number: H+-transporting two-sector ATPase
• Source (natural): Organism: Sus scrofa (pig)
• Molecular weight: Theoretical: 50.67773 KDa

Sequence

String:

AATGRIVAVI GAVVDVQFDE GLPPILNALE VQGRETRLVL EVAQHLGEST VRTIAMDGTE GLVRGQKVLD SGAPIKIPVG PETLGRIMN VIGEPIDERG PIKTKQFAAI HAEAPEFMEM SVEQEILVTG IKVVDLLAPY AKGGKIGLFG GAGVGKTVLI M ELINNVAK AHGGYSVFAG VGERTREGND LYHEMIESGV INLKDATSKV ALVYGQMNEP PGARARVALT GLTVAEYFRD QE GQDVLLF IDNIFRFTQA GSEVSALLGR IPSAVGYQPT LATDMGTMQE RITTTKKGSI TSVQAIYVPA DDLTDPAPAT TFA HLDATT VLSRAIAELG IYPAVDPLDS TSRIMDPNIV GSEHYDVARG VQKILQDYKS LQDIIAILGM DELSEEDKLT VSRA RKIQR FLSQPFQVAE VFTGHLGKLV PLKETIKGFQ QILAGEYDHL PEQAFYMVGP IEEAVAKADK LAEEH

UniProtKB: ATP synthase subunit beta

Macromolecule #4: ATP synthase subunit gamma

• Macromolecule: Name: ATP synthase subunit gamma / type: protein_or_peptide / ID: 4 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Sus scrofa (pig)
• Molecular weight: Theoretical: 30.12165 KDa

Sequence

String:

ATLKDITRRL KSIKNIQKIT KSMKMVAAAK YARAERDLKP ARVYGIGSLA LYEKADIKVP EDKKKHLIIG VSSDRGLCGA IHSSVAKQI KSEVANLTAA GKEVKIVGVG DKIRGILHRT HSDQFLVTFK EVGRKPPTFG DASVIALELL NSGYEFDEGS I IFNRFRSV ISYKTEEKPI FSLDTVASAE SMSIYDDIDA DVLRNYQEYS LANIIYYSLK ESTTSEQSAR MTAMDNASKN AS EMIDKLT LTFNRTRQAV ITKELIEIIS GAAAL

UniProtKB: ATP synthase subunit gamma

Macromolecule #5: ATP synthase F(1) complex subunit delta, mitochondrial

• Macromolecule: Name: ATP synthase F(1) complex subunit delta, mitochondrial; type: protein_or_peptide / ID: 5 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Sus scrofa (pig)
• Molecular weight: Theoretical: 13.852506 KDa

Sequence

String:

GQMSFTFASP TQVFFNGANV RQVDVPTQTG AFGILASHVP TLQVLRPGLV VVHAEDGTTS KYFVSSGSVT VNADSSVQLL AEEAVTLDM LDPGVAKANL EKAQSELLGA ADEASRAEIQ IRIEANEALV KAL

UniProtKB: ATP synthase F(1) complex subunit delta, mitochondrial

Macromolecule #6: ATP synthase F1 subunit epsilon

• Macromolecule: Name: ATP synthase F1 subunit epsilon / type: protein_or_peptide / ID: 6 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Sus scrofa (pig)
• Molecular weight: Theoretical: 5.242084 KDa

Sequence

String:

VAYWRQAGLS YIRYSQICAK AVRDALKAEF KANAEKTSGS NVKIVKV

UniProtKB: ATP synthase F1 subunit epsilon

Macromolecule #7: ATPase inhibitor, mitochondrial

• Macromolecule: Name: ATPase inhibitor, mitochondrial / type: protein_or_peptide / ID: 7 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Sus scrofa (pig)
• Molecular weight: Theoretical: 5.810329 KDa

Sequence

String:

SSDTPEGVRS GAGAVRDAGG AFGKKEQADE ERYFRARARE QLAALKKHHE NEI

UniProtKB: ATPase inhibitor, mitochondrial

Macromolecule #8: ATP synthase subunit b

• Macromolecule: Name: ATP synthase subunit b / type: protein_or_peptide / ID: 8 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Sus scrofa (pig)
• Molecular weight: Theoretical: 22.079689 KDa

Sequence

String:

FFQFLYPKTG VTGPYVLGTG LILYLLSKEI YVITAETFSA ISTIGVLVYI VKKYGASIGA FADKLNEQKI AQLEEVKQAS IKQIQDAID LEKSQQALVQ KRHYLFDVQR NNIAMALEVT YRERLHRVYK EIKNRLDYHI SVQNMMRQKE QEHMIRWVEK H VVQSISAQ QEKETIAKCI ADLKLLAKKA QAQ

UniProtKB: ATP synthase subunit b

Macromolecule #9: ATP synthase peripheral stalk subunit F6, mitochondrial

• Macromolecule: Name: ATP synthase peripheral stalk subunit F6, mitochondrial; type: protein_or_peptide / ID: 9 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Sus scrofa (pig)
• Molecular weight: Theoretical: 8.018009 KDa

Sequence

String:

KLFVDKIREY RTKRQTSGGP VDAGPEYQQD LDRELFKLKQ MYGKADMNTF PNFTFEDPKF EAVEKPQS

UniProtKB: ATP synthase peripheral stalk subunit F6, mitochondrial

Macromolecule #10: ATP synthase peripheral stalk subunit d, mitochondrial

• Macromolecule: Name: ATP synthase peripheral stalk subunit d, mitochondrial; type: protein_or_peptide / ID: 10 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Sus scrofa (pig)
• Molecular weight: Theoretical: 16.904473 KDa

Sequence

String:

RKLALKAIDW VAFGEIIPRN QKAIANSLKS WNETLSTRLA ALPEKPPAID WAYYKATVAK AGLVDDFEKK FNALKVPVPE DKYTALVDA EEQEDVKRCA EFLSLSKARI EAYEKELEKM KNIIPFDQMT IEDLNEVFPE TKLDKKKY

UniProtKB: ATP synthase peripheral stalk subunit d, mitochondrial

Macromolecule #11: ATP synthase subunit a

• Macromolecule: Name: ATP synthase subunit a / type: protein_or_peptide / ID: 11 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Sus scrofa (pig)
• Molecular weight: Theoretical: 25.054143 KDa

Sequence

String:

MNENLFASFI APTMMGLPIV TLIIMFPSLL FPTPKRLINN RTISIQQWLI QLTSKQMMAI HNQKGQTWSL MLMSLIMFIG STNILGLLP HSFTPTTQLS MNLGMAIPLW SATVFTGFRY KTKTSLAHFL PQGTPALLIP MLVIIETISL FIQPVALAVR L TANITAGH LLIHLIGGAT LALLNINTMT AFITFTILIL LTILEFAVAL IQAYVFTLLV SLYLHDNT

UniProtKB: ATP synthase F(0) complex subunit a

Macromolecule #12: ATP synthase peripheral stalk subunit OSCP, mitochondrial

• Macromolecule: Name: ATP synthase peripheral stalk subunit OSCP, mitochondrial; type: protein_or_peptide / ID: 12 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Sus scrofa (pig)
• Molecular weight: Theoretical: 20.561279 KDa

Sequence

String:

FAKLVRPPVQ IYGIEGRYAT ALYSAASKQN KLEQVEKELL RVAQILKEPK VAASIMNPYV KRSVKVKSLS DMTAKEKFSP LTSNLINLL AENGRLSSTP GVISAFSTMM SVHRGEVPCS VTTASPLDEA TLTELKTVLK SFLSKGQILK LEVKVDPSIM G GMIVRIGE KYVDMSAKTK IQKLSRAMR

UniProtKB: ATP synthase peripheral stalk subunit OSCP, mitochondrial

Macromolecule #13: ATP synthase F(0) complex subunit 8

• Macromolecule: Name: ATP synthase F(0) complex subunit 8 / type: protein_or_peptide / ID: 13 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Sus scrofa (pig)
• Molecular weight: Theoretical: 2.639243 KDa

Sequence

String:

FITITSMIMT LFILFQLKIS NY

UniProtKB: ATP synthase F(0) complex subunit 8

Macromolecule #14: ATP synthase F(0) complex subunit f, mitochondrial

• Macromolecule: Name: ATP synthase F(0) complex subunit f, mitochondrial / type: protein_or_peptide / ID: 14 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Sus scrofa (pig)
• Molecular weight: Theoretical: 9.940673 KDa

Sequence

String:

VPLKDRRLLE VKLGELPSWI LMRDFTPSGI AGAFQRGYYR YYNKYVNVKK GSVAGLSMVL AAYVVFNYCR SYKELKHERL RKYH

UniProtKB: ATP synthase F(0) complex subunit f, mitochondrial

Macromolecule #15: ATP synthase F(0) complex subunit g, mitochondrial

• Macromolecule: Name: ATP synthase F(0) complex subunit g, mitochondrial / type: protein_or_peptide / ID: 15 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Sus scrofa (pig)
• Molecular weight: Theoretical: 8.852386 KDa

Sequence

String:

KPRLATFWHY AKVELVPPTP AEIPTAIQSL KKIVNSAQTG SFKQLTVKEA LLNGLVATEV LMWFYVGEII ARAQHGIPTI

UniProtKB: ATP synthase F(0) complex subunit g, mitochondrial

Macromolecule #16: ATP synthase F(0) complex subunit e, mitochondrial

• Macromolecule: Name: ATP synthase F(0) complex subunit e, mitochondrial / type: protein_or_peptide / ID: 16 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Sus scrofa (pig)
• Molecular weight: Theoretical: 6.361458 KDa

Sequence

String:

MVPPVQVSPL IKLGRYSALF LGVAYGAKRY NYLKPRAEEE RRIAAEEKKK QDELK

UniProtKB: ATP synthase F(0) complex subunit e, mitochondrial

Macromolecule #17: ADENOSINE-5'-TRIPHOSPHATE

• Macromolecule: Name: ADENOSINE-5'-TRIPHOSPHATE / type: ligand / ID: 17 / Number of copies: 4 / Formula: ATP
• Molecular weight: Theoretical: 507.181 Da

Chemical component information

ChemComp-ATP:
ADENOSINE-5'-TRIPHOSPHATE / ATP, energy-carrying molecule*YM

Macromolecule #18: MAGNESIUM ION

• Macromolecule: Name: MAGNESIUM ION / type: ligand / ID: 18 / Number of copies: 5 / Formula: MG
• Molecular weight: Theoretical: 24.305 Da

Macromolecule #19: ADENOSINE-5'-DIPHOSPHATE

• Macromolecule: Name: ADENOSINE-5'-DIPHOSPHATE / type: ligand / ID: 19 / Number of copies: 2 / Formula: ADP
• Molecular weight: Theoretical: 427.201 Da

Chemical component information

ChemComp-ADP:
ADENOSINE-5'-DIPHOSPHATE / ADP, energy-carrying molecule*YM

Experimental details

Structure determination

• Method: cryo EM
• Processing: single particle reconstruction
• Aggregation state: particle

Sample preparation

• Concentration: 0.5 mg/mL
• Buffer: pH: 7.8
• Vitrification: Cryogen name: ETHANE / Chamber humidity: 100 %

Electron microscopy

• Microscope: TFS KRIOS
• Image recording: Film or detector model: GATAN K3 BIOQUANTUM (6k x 4k) / Average electron dose: 15.8 e/Ų
• Electron beam: Acceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
• Electron optics: Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 1.7 µm / Nominal defocus min: 1.0 µm / Nominal magnification: 81000
• Experimental equipment: Model: Titan Krios / Image courtesy: FEI Company

Image processing

• CTF correction: Type: PHASE FLIPPING AND AMPLITUDE CORRECTION
• Startup model: Type of model: NONE
• Final reconstruction: Resolution.type: BY AUTHOR / Resolution: 2.69 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: cryoSPARC / Number images used: 105447
• Initial angle assignment: Type: MAXIMUM LIKELIHOOD
• Final angle assignment: Type: MAXIMUM LIKELIHOOD

Atomic model buiding 1

• Initial model: Chain - Source name: Other / Chain - Initial model type: integrative model / Details: Genome annotation and AlphaFold

Output model

PDB-9pew:
Porcine ATP synthase with inhibitory protein IF1, DP-state