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- EMDB-70438: Enterobacter FliC filament -

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Open data


ID or keywords:

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Basic information

Entry
Database: EMDB / ID: EMD-70438
TitleEnterobacter FliC filament
Map dataPost-processed, sharpened map.
Sample
  • Complex: FliC-flagellum
    • Protein or peptide: FliC
Keywordshelical / FliC / PROTEIN FIBRIL
Biological speciesEnterobacter (bacteria)
Methodhelical reconstruction / cryo EM / Resolution: 3.6 Å
AuthorsFernandez IS
Funding support United States, 1 items
OrganizationGrant numberCountry
Howard Hughes Medical Institute (HHMI) United States
CitationJournal: To Be Published
Title: Temperate phages enhance host fitness via RNA-guided flagellar remodeling
Authors: Fernandez IS / Sternberg SH
History
DepositionApr 30, 2025-
Header (metadata) releaseJul 8, 2026-
Map releaseJul 8, 2026-
UpdateJul 8, 2026-
Current statusJul 8, 2026Processing site: RCSB / Status: Released

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Structure visualization

Supplemental images

emd_70438.png

Downloads & links

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Map

FileDownload / File: emd_70438.map.gz / Format: CCP4 / Size: 103 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
AnnotationPost-processed, sharpened map.
Projections & slices

Image control

Size
Brightness
Contrast
Others
AxesZ (Sec.)Y (Row.)X (Col.)
1.69 Å/pix.
x 300 pix.
= 506.4 Å		1.69 Å/pix.
x 300 pix.
= 506.4 Å		1.69 Å/pix.
x 300 pix.
= 506.4 Å

Surface

Projections

Slices (1/3)

Slices (1/2)

Slices (2/3)

Images are generated by Spider.

Voxel sizeX=Y=Z: 1.688 Å
Density

Histogram

Histogram (log scale)
Contour LevelBy AUTHOR: 0.016
Minimum - Maximum-0.09203662 - 0.12406428
Average (Standard dev.)0.00038845252 (±0.0054315967)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions300300300
Spacing300300300
CellA=B=C: 506.4 Å
α=β=γ: 90.0 °

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Supplemental data

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Additional map: Relion unsharpened map

Fileemd_70438_additional_1.map
AnnotationRelion unsharpened map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Half map: Relion half-map-1

Fileemd_70438_half_map_1.map
AnnotationRelion half-map-1
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Half map: Relion half-map-2

Fileemd_70438_half_map_2.map
AnnotationRelion half-map-2
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Sample components

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Entire : FliC-flagellum

EntireName: FliC-flagellum
Components
  • Complex: FliC-flagellum
    • Protein or peptide: FliC

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Supramolecule #1: FliC-flagellum

SupramoleculeName: FliC-flagellum / type: complex / ID: 1 / Parent: 0 / Macromolecule list: all
Source (natural)Organism: Enterobacter (bacteria)

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Macromolecule #1: FliC

MacromoleculeName: FliC / type: protein_or_peptide / ID: 1 / Number of copies: 23 / Enantiomer: LEVO
Source (natural)Organism: Enterobacter (bacteria)
Molecular weightTheoretical: 49.952648 KDa
Recombinant expressionOrganism: Escherichia coli (E. coli)
SequenceString: MAQVINTNSL SLITQNNINK NQSSMSTAIE RLSSGLRINS AKDDAAGQAI ANRFTSNIKG LTQAARNAND GISVAQTTEG ALSEINNNL QRIRELSVQA STGTNSDSDL ASIQDEITSR LDEIDRVSEQ TQFNGVNVLA KDGSMKIQVG ANDGQTIEID L KKIDSSTL ...String:
MAQVINTNSL SLITQNNINK NQSSMSTAIE RLSSGLRINS AKDDAAGQAI ANRFTSNIKG LTQAARNAND GISVAQTTEG ALSEINNNL QRIRELSVQA STGTNSDSDL ASIQDEITSR LDEIDRVSEQ TQFNGVNVLA KDGSMKIQVG ANDGQTIEID L KKIDSSTL GLKGLNIQTA NVSTGKELTT VPTDVTLNTT AFNTAVDGLE TGNTTATIVK GEDGSYFAKV TGATTDATKN GY YSVSIDN ASGAVTLGSK VADQTSVKSD DVALTKVTVD KAIDMTDASV AAIPGAKLYQ VTNAAGDKAY ALKGTDNKLY AAD VTNGVV TAKTINYTDA DGAAVTGANV TLGGTNGLTE VVTVGSGENA KTYKSSDLGG ANLQTQTLSA IKTSTSNPLA ALDD AISSV DKFRSSLGAI QNRLDSAVTN LNNTTTNLSE AQSRIQDADY ATEVSNMSKA QIVQQAGNSV LAKANQVPQQ VLSLL QG

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Experimental details

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Structure determination

Methodcryo EM
Processinghelical reconstruction
Aggregation statehelical array

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Sample preparation

BufferpH: 7
VitrificationCryogen name: ETHANE

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Electron microscopy

MicroscopeTFS KRIOS
Image recordingFilm or detector model: GATAN K3 BIOQUANTUM (6k x 4k) / Average electron dose: 60.0 e/Å2
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: OTHER / Imaging mode: BRIGHT FIELD / Nominal defocus max: 2.5 µm / Nominal defocus min: 0.5 µm
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

Final reconstructionApplied symmetry - Helical parameters - Δz: 24.35 Å
Applied symmetry - Helical parameters - Δ&Phi: -33 °
Applied symmetry - Helical parameters - Axial symmetry: C1 (asymmetric)
Resolution.type: BY AUTHOR / Resolution: 3.6 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 47000
CTF correctionType: PHASE FLIPPING AND AMPLITUDE CORRECTION
Startup modelType of model: OTHER
Final angle assignmentType: NOT APPLICABLE

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