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- EMDB-69918: Xanthomonas citri pv. glycines 50S subunit -

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Basic information

Entry
Database: EMDB / ID: EMD-69918
TitleXanthomonas citri pv. glycines 50S subunit
Map dataXanthomonas campestris 50S map
Sample
  • Complex: 50S rebosomal subunit of Xanthomonas citri pv. glycine
    • RNA: x 2 types
    • Protein or peptide: x 27 types
KeywordsPhytopathogen / Xanthomonas citri pv. glycines / 50S subunit / RIBOSOME
Function / homology
Function and homology information


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Similarity search - Component
Biological speciesXanthomonas citri pv. glycines (bacteria)
Methodsingle particle reconstruction / cryo EM / Resolution: 3.0 Å
AuthorsSengupta S / Anand R
Funding support India, 1 items
OrganizationGrant numberCountry
Science and Engineering Research Board (SERB) India
CitationJournal: Acs Omega / Year: 2026
Title: Structure of 50S ribosomal subunit from phytopathogen Xanthomonas citri pv. glycines
Authors: Sengupta S / Tripathy J / Bandyopadhay N / Gautam S / Anand R
History
DepositionMar 25, 2026-
Header (metadata) releaseAug 26, 2026-
Map releaseAug 26, 2026-
UpdateAug 26, 2026-
Current statusAug 26, 2026Processing site: PDBj / Status: Released

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Structure visualization

Supplemental images

emd_69918.png

Downloads & links

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Map

FileDownload / File: emd_69918.map.gz / Format: CCP4 / Size: 244.1 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
AnnotationXanthomonas campestris 50S map
Projections & slices

Image control

Size
Brightness
Contrast
Others
AxesZ (Sec.)Y (Row.)X (Col.)
0.85 Å/pix.
x 400 pix.
= 340. Å		0.85 Å/pix.
x 400 pix.
= 340. Å		0.85 Å/pix.
x 400 pix.
= 340. Å

Surface

Projections

Slices (1/3)

Slices (1/2)

Slices (2/3)

Images are generated by Spider.

Voxel sizeX=Y=Z: 0.85 Å
Density

Histogram

Histogram (log scale)
Contour LevelBy AUTHOR: 0.00566
Minimum - Maximum-0.018232686 - 0.04674932
Average (Standard dev.)0.00022268068 (±0.001719109)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions400400400
Spacing400400400
CellA=B=C: 340.0 Å
α=β=γ: 90.0 °

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Supplemental data

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Half map: #2

Fileemd_69918_half_map_1.map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Half map: #1

Fileemd_69918_half_map_2.map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Sample components

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Entire : 50S rebosomal subunit of Xanthomonas citri pv. glycine

EntireName: 50S rebosomal subunit of Xanthomonas citri pv. glycine
Components
  • Complex: 50S rebosomal subunit of Xanthomonas citri pv. glycine
    • RNA: RNA (2797-MER)
    • Protein or peptide: Large ribosomal subunit protein bL27
    • Protein or peptide: Large ribosomal subunit protein bL33
    • Protein or peptide: Large ribosomal subunit protein bL34
    • Protein or peptide: Large ribosomal subunit protein bL35
    • Protein or peptide: Large ribosomal subunit protein bL36
    • Protein or peptide: Large ribosomal subunit protein uL2
    • Protein or peptide: Large ribosomal subunit protein uL3
    • Protein or peptide: Large ribosomal subunit protein uL4
    • Protein or peptide: Large ribosomal subunit protein uL6
    • Protein or peptide: Large ribosomal subunit protein bL9
    • Protein or peptide: Large ribosomal subunit protein uL13
    • Protein or peptide: Large ribosomal subunit protein uL14
    • Protein or peptide: Large ribosomal subunit protein uL15
    • Protein or peptide: Large ribosomal subunit protein uL16
    • Protein or peptide: Large ribosomal subunit protein bL17
    • Protein or peptide: Large ribosomal subunit protein uL18
    • Protein or peptide: Large ribosomal subunit protein bL19
    • Protein or peptide: Large ribosomal subunit protein bL20
    • Protein or peptide: Large ribosomal subunit protein bL21
    • Protein or peptide: Large ribosomal subunit protein uL22
    • Protein or peptide: Large ribosomal subunit protein uL23
    • Protein or peptide: Large ribosomal subunit protein uL24
    • Protein or peptide: Large ribosomal subunit protein bL25
    • Protein or peptide: Large ribosomal subunit protein bL28
    • Protein or peptide: Large ribosomal subunit protein uL29
    • Protein or peptide: Large ribosomal subunit protein uL30
    • RNA: RNA (117-MER)
    • Protein or peptide: Large ribosomal subunit protein bL32

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Supramolecule #1: 50S rebosomal subunit of Xanthomonas citri pv. glycine

SupramoleculeName: 50S rebosomal subunit of Xanthomonas citri pv. glycine
type: complex / ID: 1 / Parent: 0 / Macromolecule list: #6, #26, #2-#5, #8-#25, #27-#29, #7, #1
Source (natural)Organism: Xanthomonas citri pv. glycines (bacteria)

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Macromolecule #1: Large ribosomal subunit protein bL32

MacromoleculeName: Large ribosomal subunit protein bL32 / type: protein_or_peptide / ID: 1 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Xanthomonas citri pv. glycines (bacteria)
Molecular weightTheoretical: 7.146035 KDa
SequenceString:
MAVQKSRVTP SRRGQRRSHD ALTAKQLSTD PTSGEIHLRH HITADGYYRG KKVIATKSSA VQED

UniProtKB: UNIPROTKB: A0AAX0HTU4

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Macromolecule #2: Large ribosomal subunit protein bL33

MacromoleculeName: Large ribosomal subunit protein bL33 / type: protein_or_peptide / ID: 2 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Xanthomonas citri pv. glycines (bacteria)
Molecular weightTheoretical: 6.422719 KDa
SequenceString:
MAKGKRDKIR MISSAATGHF YTTDKNKKNT PGKMEMMKYD PVVRKHVMYK EGKIK

UniProtKB: UNIPROTKB: A0AAX0HWN5

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Macromolecule #3: Large ribosomal subunit protein bL34

MacromoleculeName: Large ribosomal subunit protein bL34 / type: protein_or_peptide / ID: 3 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Xanthomonas citri pv. glycines (bacteria)
Molecular weightTheoretical: 5.327328 KDa
SequenceString:
MATKRTFQPS NLKRARDHGF RARMATADGR KILARRRAKG RKRLSA

UniProtKB: UNIPROTKB: A0AAX0HWA1

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Macromolecule #4: Large ribosomal subunit protein bL35

MacromoleculeName: Large ribosomal subunit protein bL35 / type: protein_or_peptide / ID: 4 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Xanthomonas citri pv. glycines (bacteria)
Molecular weightTheoretical: 7.583993 KDa
SequenceString:
MPKIKTNRAA AKRFRKTASG KYKCGHANRS HILTKKATKR KRNLRQTNHV RAEDAGRLDR MLPYL

UniProtKB: UNIPROTKB: A0AAX0I0L4

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Macromolecule #5: Large ribosomal subunit protein bL36

MacromoleculeName: Large ribosomal subunit protein bL36 / type: protein_or_peptide / ID: 5 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Xanthomonas citri pv. glycines (bacteria)
Molecular weightTheoretical: 4.887983 KDa
SequenceString:
MKVLSSLKSA KTRHRDCKVV RRRGKVFVIC KSNPRFKARQ R

UniProtKB: UNIPROTKB: A0AAX0I1I4

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Macromolecule #8: Large ribosomal subunit protein uL2

MacromoleculeName: Large ribosomal subunit protein uL2 / type: protein_or_peptide / ID: 8 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Xanthomonas citri pv. glycines (bacteria)
Molecular weightTheoretical: 29.96159 KDa
SequenceString: MPLMKFKPTS PGRRSAVRVV TPDLHKGAPH AALLDSQSKS GGRNHHGRIT VRHVGGGHKQ HYRIIDFKRN KEGIPARVER IEYDPNRTA HIALLCYVDG ERRYIIAPKG LKAGDQVIAG ANAPIKTGNT LPLRNIPVGT TVHGIELKPG KGAQIARAAG A AVQLVARE ...String:
MPLMKFKPTS PGRRSAVRVV TPDLHKGAPH AALLDSQSKS GGRNHHGRIT VRHVGGGHKQ HYRIIDFKRN KEGIPARVER IEYDPNRTA HIALLCYVDG ERRYIIAPKG LKAGDQVIAG ANAPIKTGNT LPLRNIPVGT TVHGIELKPG KGAQIARAAG A AVQLVARE GIYATLRLRS GEMRKVPVEC RATIGEVGND EHNLEKLGKA GAKRWRGVRP TVRGAAMNPV DHPHGGGEAK AG QGNPHPV TPWGVPTKGY KTRKNKRTQQ FIVRDRRG

UniProtKB: UNIPROTKB: A0AAX0HUI0

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Macromolecule #9: Large ribosomal subunit protein uL3

MacromoleculeName: Large ribosomal subunit protein uL3 / type: protein_or_peptide / ID: 9 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Xanthomonas citri pv. glycines (bacteria)
Molecular weightTheoretical: 22.90724 KDa
SequenceString: MTKKYSLGFV GRKAGMSRVF TEDGRSVPVT LIEATPNRIA QIKTVEVDGY SAVQVTVGAR RAALVNKPAA GHFAKAKVEA GRGLWEFRV EDAQLGDFAV GGEIKADIFE VGQKVDVQGV TKGKGFQGTI KRYNFRMGDA THGNSLSHRA PGSLGQRQTP G RVFPGKKM ...String:
MTKKYSLGFV GRKAGMSRVF TEDGRSVPVT LIEATPNRIA QIKTVEVDGY SAVQVTVGAR RAALVNKPAA GHFAKAKVEA GRGLWEFRV EDAQLGDFAV GGEIKADIFE VGQKVDVQGV TKGKGFQGTI KRYNFRMGDA THGNSLSHRA PGSLGQRQTP G RVFPGKKM SGHMGAVQQS TQNLEVVKVD VERGLIAIRG AVPGAAGGDV IVRPASKA

UniProtKB: UNIPROTKB: A0AAX0HUK4

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Macromolecule #10: Large ribosomal subunit protein uL4

MacromoleculeName: Large ribosomal subunit protein uL4 / type: protein_or_peptide / ID: 10 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Xanthomonas citri pv. glycines (bacteria)
Molecular weightTheoretical: 21.80315 KDa
SequenceString: MELVITGSNN KVSVSDAVFG REFSEDLVHQ VVVAYRNAGR AGTKAQKTRS EVAGTTKKSK KQKGGGARHG ALTAPIFVGG GVTFAAKPR SFEQKVNRKM YRAAICAIFS ELNRQGRLMI VDAFDLEATK TKGLIEKLKG LEVGKRPLIV TEEASEHLYL S ARNLPYVQ ...String:
MELVITGSNN KVSVSDAVFG REFSEDLVHQ VVVAYRNAGR AGTKAQKTRS EVAGTTKKSK KQKGGGARHG ALTAPIFVGG GVTFAAKPR SFEQKVNRKM YRAAICAIFS ELNRQGRLMI VDAFDLEATK TKGLIEKLKG LEVGKRPLIV TEEASEHLYL S ARNLPYVQ VRDVQGLDPV ALVGADTVVI TADAVKKVEE WLA

UniProtKB: UNIPROTKB: A0AAX0HUR7

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Macromolecule #11: Large ribosomal subunit protein uL6

MacromoleculeName: Large ribosomal subunit protein uL6 / type: protein_or_peptide / ID: 11 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Xanthomonas citri pv. glycines (bacteria)
Molecular weightTheoretical: 18.304418 KDa
SequenceString:
MSRVAKKPVS LPKGVELNVQ PGLVSVKGPK GTLTLPKPAG VEIAIDGGVA TLSANDPSQI ALTGTVRAIL ANMVKGVSEG FERKLELVG VGYRAAMQGK DLSLALGFSH PLVFVAPEGI TLSTPTQTEI VVQGADKQRV GEVAAKIRGF RPPEPYKGKG V KYAGEVII RKEAKKA

UniProtKB: UNIPROTKB: A0AAX0HUH5

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Macromolecule #12: Large ribosomal subunit protein bL9

MacromoleculeName: Large ribosomal subunit protein bL9 / type: protein_or_peptide / ID: 12 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Xanthomonas citri pv. glycines (bacteria)
Molecular weightTheoretical: 15.716693 KDa
SequenceString:
MDLILLQKVT NLGNLGDKVS VKPGYGRNFL VPQGKAVPAT AANVEAFETK RAEYEAKANS ILAEAQSRAT KFEGASVTIG AHASTEGKL YGSVGPRDIA EAFTAAGLPL EKSEVILGEG AFRNVGEYDV VLHLHADVET TVKVIVESDA

UniProtKB: UNIPROTKB: A0AAX0I4I2

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Macromolecule #13: Large ribosomal subunit protein uL13

MacromoleculeName: Large ribosomal subunit protein uL13 / type: protein_or_peptide / ID: 13 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Xanthomonas citri pv. glycines (bacteria)
Molecular weightTheoretical: 16.110677 KDa
SequenceString:
MTTFTAKSET VQRDWYLVDA AGKTLGRLST ELARRLRGKH KPVYTPHVDT GDYLVVINAE KIVVTGNKLK DKKYHRFTGY IGNLKTESL EQALQRHPER VIEIAVKGML PKGPLGRTMY RKLKVYSGAE HPHAAQQPQV LDI

UniProtKB: UNIPROTKB: A0AAX0HVE2

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Macromolecule #14: Large ribosomal subunit protein uL14

MacromoleculeName: Large ribosomal subunit protein uL14 / type: protein_or_peptide / ID: 14 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Xanthomonas citri pv. glycines (bacteria)
Molecular weightTheoretical: 13.547967 KDa
SequenceString:
MIQMQSYLDV ADNSGAKEVM CIKVLGGSKR RYAHIGDIIK VTVKDAIPRG KVKKGEVYDA VVVRTRKGVR RPDGSLIRFD GNAAVLLNN KQEPIGTRIF GPVTRELRSE KFMKIVSLAP EVL

UniProtKB: UNIPROTKB: A0AAX0HU63

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Macromolecule #15: Large ribosomal subunit protein uL15

MacromoleculeName: Large ribosomal subunit protein uL15 / type: protein_or_peptide / ID: 15 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Xanthomonas citri pv. glycines (bacteria)
Molecular weightTheoretical: 15.364844 KDa
SequenceString:
MTLRLNDLKP ADGARTERTR VGRGIGSGLG KTAGRGHKGS FARKGGGKIK AGFEGGQTPM QRRLPKIGFR SKMARDTAEV LSYQLDKLD AGDVDFAALR AANLVPSRAK KAKIVLKGEL SKKFVLKGVA ATAGAKAAIE AAGGSVEE

UniProtKB: UNIPROTKB: A0AAX0HTY3

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Macromolecule #16: Large ribosomal subunit protein uL16

MacromoleculeName: Large ribosomal subunit protein uL16 / type: protein_or_peptide / ID: 16 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Xanthomonas citri pv. glycines (bacteria)
Molecular weightTheoretical: 15.529108 KDa
SequenceString:
MLQPKRTKYR KMHKGRNDGL AWSGNAVSFG EYGLKATAHG QLTARQIEAA RRTISRHVKK GGKMWIRVFP DKPITKKPIE VRMGSGKGN VEYWVAQIQP GRMIYEIEGI PEETAREAFR LAAAKLSVTT TFVTRTVR

UniProtKB: UNIPROTKB: A0AAX0HUF6

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Macromolecule #17: Large ribosomal subunit protein bL17

MacromoleculeName: Large ribosomal subunit protein bL17 / type: protein_or_peptide / ID: 17 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Xanthomonas citri pv. glycines (bacteria)
Molecular weightTheoretical: 14.345796 KDa
SequenceString:
MRHQKSGRKF NRTSAHREAM FRNMAASLFK HELIKTTLPK AKELRRVAEP LITIGKVDGV ANRRLAFARL RDKEAVGKLF VELGPRYAT RPGGYLRILK AGFRAGDNAP MAYVELVDRP VVAEEVAE

UniProtKB: UNIPROTKB: A0AAX0HU83

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Macromolecule #18: Large ribosomal subunit protein uL18

MacromoleculeName: Large ribosomal subunit protein uL18 / type: protein_or_peptide / ID: 18 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Xanthomonas citri pv. glycines (bacteria)
Molecular weightTheoretical: 12.82777 KDa
SequenceString:
MSINKNIARL RRAKSTRAHI RELGVARLSV LRTGQHLYAQ VFTADGSKVI AAANTLQADV KDGLKNGKNS DAAAKVGKLI AERAKAAGI EKVAFDRSGY RYHGRIKALA DAAREGGLQF

UniProtKB: UNIPROTKB: A0AAX0HUQ7

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Macromolecule #19: Large ribosomal subunit protein bL19

MacromoleculeName: Large ribosomal subunit protein bL19 / type: protein_or_peptide / ID: 19 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Xanthomonas citri pv. glycines (bacteria)
Molecular weightTheoretical: 14.78098 KDa
SequenceString:
MSKLNKTILA DFEAAQIQRK LPEFNQGDTV VVNVKVKEGN RERVQAYEGV VIGTKNAGLN SSFTVRKISH GFGVERVFQT HSAIIDSVE VKRRGKVRAG KLYYLRGLEG KAARIKEDLA AAAQAKAARQ AAAKAE

UniProtKB: UNIPROTKB: A0AAX0HU77

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Macromolecule #20: Large ribosomal subunit protein bL20

MacromoleculeName: Large ribosomal subunit protein bL20 / type: protein_or_peptide / ID: 20 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Xanthomonas citri pv. glycines (bacteria)
Molecular weightTheoretical: 13.385935 KDa
SequenceString:
MARVKRGVQA RRRHKKILTL AKGYYNARRK VFRVAKQAVI KAQQYAYIGR KQKKRNFRSL WITRINAAAR INGLSYSRFM NGLLKAGIT LDRKVLADIA VHDAAGFAAL AEKAKGALAA

UniProtKB: UNIPROTKB: A0AAX0I0J6

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Macromolecule #21: Large ribosomal subunit protein bL21

MacromoleculeName: Large ribosomal subunit protein bL21 / type: protein_or_peptide / ID: 21 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Xanthomonas citri pv. glycines (bacteria)
Molecular weightTheoretical: 11.823744 KDa
SequenceString:
MYAVLVTGGK QYRVAQGETL RVEKLEVEAG NEIKFDTILM LGDSDGIKLG DALKGASVTA KVVAHGRADK VRIIKFRRRK HHMKRQGHR QHYTEIEITG IAGGDKK

UniProtKB: UNIPROTKB: A0AAX0HU45

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Macromolecule #22: Large ribosomal subunit protein uL22

MacromoleculeName: Large ribosomal subunit protein uL22 / type: protein_or_peptide / ID: 22 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Xanthomonas citri pv. glycines (bacteria)
Molecular weightTheoretical: 12.098252 KDa
SequenceString:
MEAKAILRTA RISPQKARLV ADQVRGLSAE RAVNLLKFSD KKAAHLIKKV VESAIANAEN NQGADVDELK VKTIMVDEGP SLKRFMARA KGRGTRILKR TSHITVVVGA AK

UniProtKB: UNIPROTKB: A0AAX0HU92

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Macromolecule #23: Large ribosomal subunit protein uL23

MacromoleculeName: Large ribosomal subunit protein uL23 / type: protein_or_peptide / ID: 23 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Xanthomonas citri pv. glycines (bacteria)
Molecular weightTheoretical: 11.06262 KDa
SequenceString:
MSSNEKIFSV LRAPRVSEKT ARLQEISNQY VFEVSNEATK ADVKAAVEQL FDVKVKAVNV VNVKGKSKSF RNRAGNRGNW RKAYVRLVD GQSIDVTAKA

UniProtKB: UNIPROTKB: A0AAX0HUB3

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Macromolecule #24: Large ribosomal subunit protein uL24

MacromoleculeName: Large ribosomal subunit protein uL24 / type: protein_or_peptide / ID: 24 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Xanthomonas citri pv. glycines (bacteria)
Molecular weightTheoretical: 11.246825 KDa
SequenceString:
MANRIKKGDQ VVINTGKDKG KQGEVVRVDG DRVIVSNANV IKRHTKPNPQ AGVAGGVVER EASIHISNVN IVNPATGKGE RVGFKVLED GRKLRVFRSS GEALDA

UniProtKB: UNIPROTKB: A0AAX0HUK9

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Macromolecule #25: Large ribosomal subunit protein bL25

MacromoleculeName: Large ribosomal subunit protein bL25 / type: protein_or_peptide / ID: 25 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Xanthomonas citri pv. glycines (bacteria)
Molecular weightTheoretical: 23.38149 KDa
SequenceString: MAKTHEIKVE RRADEGKGAS RRLRHAGVIP AIVYGGELEP VSIQLNHEQI WLAQQNEWFY SSILDLNLNG DVQQVLLRDM QRHPFKQLI MHIDFQRVSA NEKLSAAVPL HFINEASSPA GKSSEVVVTH ELNEVQVVCL PKDLPEFIEV DLGALEVGNV I HLSEIKLP ...String:
MAKTHEIKVE RRADEGKGAS RRLRHAGVIP AIVYGGELEP VSIQLNHEQI WLAQQNEWFY SSILDLNLNG DVQQVLLRDM QRHPFKQLI MHIDFQRVSA NEKLSAAVPL HFINEASSPA GKSSEVVVTH ELNEVQVVCL PKDLPEFIEV DLGALEVGNV I HLSEIKLP AGVEIPELKL GKEHDVAVVA AKHVRIEEDD AAGEEGSEGA ETK

UniProtKB: UNIPROTKB: A0AAX0HVG2

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Macromolecule #26: Large ribosomal subunit protein bL27

MacromoleculeName: Large ribosomal subunit protein bL27 / type: protein_or_peptide / ID: 26 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Xanthomonas citri pv. glycines (bacteria)
Molecular weightTheoretical: 9.119448 KDa
SequenceString:
MAHKKGVGSS RNGRDSNPKY LGVKIFGGQA IDAGNIIVRQ RGTQFHPGAG VGLGRDHTLF ALVDGKVEFS TKGPKKRRTV SVVAEA

UniProtKB: UNIPROTKB: A0AAX0HUH8

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Macromolecule #27: Large ribosomal subunit protein bL28

MacromoleculeName: Large ribosomal subunit protein bL28 / type: protein_or_peptide / ID: 27 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Xanthomonas citri pv. glycines (bacteria)
Molecular weightTheoretical: 9.112517 KDa
SequenceString:
MSRVCQVSGK RVQTGNNVSH ANNRTRRRFL PNLHERRFWV ASENRWVKLR VSAHALRTID KNGIDAVLKE LRARGEKV

UniProtKB: UNIPROTKB: A0AAX0HWI5

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Macromolecule #28: Large ribosomal subunit protein uL29

MacromoleculeName: Large ribosomal subunit protein uL29 / type: protein_or_peptide / ID: 28 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Xanthomonas citri pv. glycines (bacteria)
Molecular weightTheoretical: 7.25548 KDa
SequenceString:
MDIKQLREKS ADELKAHLTD LRKEQFSLRM QQVTGQLPKT HETRRVRREI ARVKHLLGST K

UniProtKB: UNIPROTKB: A0AAX0HU43

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Macromolecule #29: Large ribosomal subunit protein uL30

MacromoleculeName: Large ribosomal subunit protein uL30 / type: protein_or_peptide / ID: 29 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Xanthomonas citri pv. glycines (bacteria)
Molecular weightTheoretical: 7.223461 KDa
SequenceString:
MAKDTNKTVK VRLVRGLRGT QSRHRLSVRA LGLNKLNDVR ELKDSPQVRG LINTVHYLVK VEE

UniProtKB: UNIPROTKB: A0AAX0HUJ8

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Macromolecule #6: RNA (2797-MER)

MacromoleculeName: RNA (2797-MER) / type: rna / ID: 6 / Number of copies: 1
Source (natural)Organism: Xanthomonas citri pv. glycines (bacteria)
Molecular weightTheoretical: 933.660812 KDa
SequenceString: GGUCAAGCGA AUAAGCGCAC ACGGUGGAUG CCUAGGCGGU CAGAGGCGAU GAAGGACGUG GUAGCCUGCG AAAAGUGUCG GGGAGCUGG CAACAAGCUU UGAUCCGGCA AUAUCCGAAU GGGGAAACCC ACUGCUUCGG CAGUAUCCUG CAGUGAAUUC A UAGCUGCU ...String:
GGUCAAGCGA AUAAGCGCAC ACGGUGGAUG CCUAGGCGGU CAGAGGCGAU GAAGGACGUG GUAGCCUGCG AAAAGUGUCG GGGAGCUGG CAACAAGCUU UGAUCCGGCA AUAUCCGAAU GGGGAAACCC ACUGCUUCGG CAGUAUCCUG CAGUGAAUUC A UAGCUGCU GGAAGCGAAC CCGGUGAACU GAAAUAUCUA AGUAACCGGA GGAAAAGAAA UCAACCGAGA UUCCCUAAGU AG UGACGAG CGAACGGGGA ACAGCCCUUA AGCUGGAAUG GCUUUAGAAA AACAAUCUGG AAAGAUUGGC CAUAGAAGGU GAU AGCCCU GUAUUUAAAA GGGCCACUCC AGUGAAGACG AGUAGGGCGG GGCACGUGAA ACCCUGUCUG AAUAUGGGGG GACC AUCCU CCAAGGCUAA AUACUCCUGA CCGACCGAUA GUGAACCAGU ACCGUGAGGG AAAGGCGAAA AGAACCCCGG AGAGG GGAG UGAAAUAGAU CCUGAAACCG UGUGCGUACA AGCAGUAGGA GCUCGCAAGA GUGACUGCGU ACCUUUUGUA UAAUGG GUC AGCGACUUAC UGUUCGUGGC AAGCUUAACC GUAUAGGGGA GGCGAAGGGA AACCGAGUCU GAUAAGGGCG CAUAGUC GC GGGCAGUAGA CCCGAAACCG GGUGAUCUAG UCAUGGCCAG GGUGAAGGUG CCGUAACAGG UACUGGAGGC CCGAACCC A CGUCUGUUGC AAAAGACGGG GAUGAGCUGU GAUUAGGAGU GAAAAGCUAA UCGAACCCGG AGAUAGCUGG UUCUCCUCG AAAGCUAUUU AGGUAGCGCC UCAUAUGUAU CCUCUCGGGG GUAGAGCACU GUUAUGGCUA GGGGGUCAUC GCGACUUACC AAACCAUUG CAAACUCCGA AUACCGAGAC GGACUGUAUG GGAGACACAC GGCGGGUGCU AACGUCCGUC GUGAAAAGGG A AACAACCC AGACCCACAG CUAAGGUCCC AAAUUCACUG CUAAGUGGAA AACGAUGUGG AAAGGCAUAG ACAGCCAGGA GG UUGGCUU AGAAGCAGCC ACCCUUUAAA GAAAGCGUAA UAGCUCACUG GUCGAGUCGG UCUGCGCGGA AGAUUUAACG GGG CUAAGC AGUGAACCGA AGCUUGGGGU GCAUAAAACG UGUUUUAUGC GCGGUAGAGG AGCGUUCCGU AAGCCUGCGA AGGU GGAUU GAGAAGUCUG CUGGAGGUAU CGGAAGUGCG AAUGCUGACA UGAGUAACGA UAAUGCGGGU GAAAAGCCCG CACGC CGAA AGCCCAAGGU UUCCUUGCGC AACGUUAAUC GACGCAGGGU UAGUCGGUCC CUAAGGCGAG GGCGAAAGCC GUAGUC GAU GGGAAGCAGG UUAAUAUUCC UGCACCUCGC GUGAGUGCGA UGGAGGGACG GAGAAGGUUA GGUGUACCGG GCGUUGG UU GUCCCGGGGA AAGGCGGUAG GUUUGGAUCU UUGGCAAAUC CGGGAUCCUU UAAGACCGAG CACCGAGACG AGCCUUUU U GGCGAAGUCA CUGAUACCAC GCUUCCAGGA AAAGCUCCUA AGCUUCAGCU CACGAAGACC GUACCGUAAA CCGACACAG GUGGGUAGGA UGAGAAUUCU CAGGCGCUUG AGAGAACUCG GGUGAAGGAA CUAGGCAACA UGGCACCGUA ACUUCGGGAG AAGGUGCAC CCUUUUUGGU GGCUCAUGCG AGCUAUAGCU GAAGAGGGUC GCAGAAACCA GGCCGCUGCG ACUGUUUAUC A AAAACACA GCACUCUGCA AACACGAAAG UGGACGUAUA GGGUGUGACG CCUGCCCGGU GCUGGAAGGU UAAUUGAUGG GG UCAGCCG CAAGGCGAAG CUCUUGAUCG AAGCCCCAGU AAACGGCGGC CGUAACUAUA ACGGUCCUAA GGUAGCGAAA UUC CUUGUC GGGUAAGUUC CGACCUGCAC GAAUGGCGUA ACGACAGCGG CGCUGUCUCC ACCCGAGACU CAGUGAAAUU GAAA UCGCU GUGAAGAUGC AGCGUUCCCG UGGCAAGACG GAAAGACCCC GUGAACCUUU ACUAUAGCUU UACACUGAAC GUUGA GUUC GUCUGUGUAG GAUAGGUGGG AGGCUAUGAA ACUGUGGCGC UAGCUGCAGU GGAGCCAUCC UUGAAAUACC ACCCUG UCG UGCUUGACGU UCUAACCUAG GUCCGUUAUC CGGAUCAGGG ACCGUGUAUG GUGGGUAGUU UGACUGGGGC GGUCUCC UC CUAAAGAGUA ACGGAGGAGC ACGAAGGUAC GCUCAGCGCG GUCGGACAUC GCGCACUGUG UGCAAAGGCA UAAGCGUG C UUGACUGCAA GAUCGACGGA UCAAGCAGGU ACGAAAGUAG GUCUUAGUGA UCCGGUGGUU CUGUAUGGAA GGGCCAUCG CUCAACGGAU AAAAGGUACU CCGGGGAUAA CAGGCUGAUA CCGCCCAAGA GUUCAUAUCG ACGGCGGUGU UUGGCACCUC GAUGUCGGC UCAUCACAUC CUGGGGCUGU AGUCGGUCCC AAGGGUAUGG CUGUUCGCCA UUUAAAGUGG UACGCGAGCU G GGUUCAGA ACGUCGUGAG ACAGUUCGGU CCCUAUCUGC CAUGGGCGUU GGAAGUUUGA GAGGGGCUGC UCCUAGUACG AG AGGACCG GAGUGGACGA ACCUCUGGUG UUCCGGUUGU CACGCCAGUG GCAUUGCCGG GUAGCUAUGU UCGGAAGCGA UAA CCGCUG AAAGCAUCUA AGCGGGAAGC GCGCCUCAAG AUGAGACUUC CCGGGGCACA AGCCCCCUAA AGGAACCAUG UAGA CUACG UGGUUGAUAG GUCAGGUGUG UAAGUACAGC AAUGUAUUGA GCUAACUGAU ACUAAUGAUC CGUGCGGCUU GACCA U

GENBANK: GENBANK: CP072265.1

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Macromolecule #7: RNA (117-MER)

MacromoleculeName: RNA (117-MER) / type: rna / ID: 7 / Number of copies: 1
Source (natural)Organism: Xanthomonas citri pv. glycines (bacteria)
Molecular weightTheoretical: 39.033234 KDa
SequenceString:
UCUCCCUGGU GAAAUUAGCG CUGUGGAACC ACCCGAUCCC AUCCCGAACU CGGAAGUGAA ACGCAGCUGC GCCGAUGGUA GUGUGGCUC AAGCCAUGCG AGAGUAGGUC AUCGCCAGGG GC

GENBANK: GENBANK: CP041781.1

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

BufferpH: 7.5
VitrificationCryogen name: ETHANE

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Electron microscopy

MicroscopeTFS KRIOS
Specialist opticsEnergy filter - Name: GIF Bioquantum
Image recordingFilm or detector model: GATAN K3 BIOCONTINUUM (6k x 4k) / Average electron dose: 43.0 e/Å2
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 2.0 µm / Nominal defocus min: 0.5 µm
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

CTF correctionSoftware - Name: CTFFIND / Type: PHASE FLIPPING AND AMPLITUDE CORRECTION
Startup modelType of model: PDB ENTRY
PDB model - PDB ID:

6spd

Final reconstructionResolution.type: BY AUTHOR / Resolution: 3.0 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: RELION (ver. 4.0.1) / Number images used: 252876
Initial angle assignmentType: MAXIMUM LIKELIHOOD
Final angle assignmentType: MAXIMUM LIKELIHOOD
FSC plot (resolution estimation)

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