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- EMDB-65259: Cryo-EM structure of the dimeric Elapor1 mutant -

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Basic information

Entry
Database: EMDB / ID: EMD-65259
TitleCryo-EM structure of the dimeric Elapor1 mutant
Map data
Sample
  • Complex: Elapor1 mutant
    • Protein or peptide: Endosome/lysosome-associated apoptosis and autophagy regulator 1
  • Ligand: 2-acetamido-2-deoxy-beta-D-glucopyranose
KeywordsTethering factor / MEMBRANE PROTEIN
Function / homology
Function and homology information


positive regulation of autophagosome assembly / autophagosome assembly / cellular response to starvation / trans-Golgi network / late endosome / lysosome / plasma membrane
Similarity search - Function
EIG121-like / : / : / : / : / : / ELAPOR1-like, galactose binding domain / ELAPOR1-like, galactose-binding domain / ELAPOR1-like, mannose 6-phosphate receptor homology domain / ELAPOR1-like, C-terminal domain ...EIG121-like / : / : / : / : / : / ELAPOR1-like, galactose binding domain / ELAPOR1-like, galactose-binding domain / ELAPOR1-like, mannose 6-phosphate receptor homology domain / ELAPOR1-like, C-terminal domain / ELAPOR1-like, TNF receptor-like / Mannose-6-phosphate receptor binding domain superfamily / MRH domain / MRH domain profile. / Putative ephrin-receptor like / Growth factor receptor cysteine-rich domain superfamily
Similarity search - Domain/homology
Endosome/lysosome-associated apoptosis and autophagy regulator 1
Similarity search - Component
Biological speciesMus musculus (house mouse)
Methodsingle particle reconstruction / cryo EM / Resolution: 3.6 Å
AuthorsMa J / Zheng S
Funding support China, 1 items
OrganizationGrant numberCountry
National Science Foundation (NSF, China) China
CitationJournal: Proc Natl Acad Sci U S A / Year: 2025
Title: ELAPOR1 is a copper-dependent tethering factor driving proacrosomal vesicle fusion during acrosome biogenesis.
Authors: Tianyu Shao / Jiahao Ma / Xinshui Tan / Hongcheng Shan / Dan Xu / Kexin Zhang / Sanduo Zheng / Fengchao Wang /
Abstract: The acrosome is a crucial organelle essential for sperm function and male fertility. During acrosome biogenesis, numerous proacrosomal vesicles (PAVs) are transported to the concave region of the ...The acrosome is a crucial organelle essential for sperm function and male fertility. During acrosome biogenesis, numerous proacrosomal vesicles (PAVs) are transported to the concave region of the nuclear membrane and fuse to form the acrosome. However, the mechanisms governing the fusion of PAVs to form the acrosome remain poorly understood. Here, we identify endosome-lysosome associated apoptosis and autophagy regulator 1 (ELAPOR1), a conserved protein, as a key factor in PAVs fusion during acrosome biogenesis. Male mice lacking () are infertile, exhibiting defective acrosome biogenesis and a globozoospermia-like phenotype. Using cryo-electron microscopy revealed that ELAPOR1 forms a square planar homodimer in cis, which assembles into a trans-tetramer via head-to-head homophilic interactions dependent on copper chelation. Notably, ELAPOR1 exhibits dual membrane orientation, with a predicted N - C topology and a noncanonical N - C topology in vesicles. The noncanonical N - C topology enables ELAPOR1 to function as a tethering factor bridging vesicles through head-to-head homophilic interactions. A mutant ELAPOR1 (ELAPOR1) incapable of copper chelation forms cis homodimers but fails to mediate homophilic interactions in vitro, leading to defective PAVs fusion in mice, phenocopying the -deficient mice. Additionally, ELAPOR1 was shown to interact with soluble N-ethylmaleimide sensitive factor attachment protein receptors protein STX12. Conditional knockout of in germ cells resulted in similar defects in acrosome biogenesis. Collectively, our findings suggest that ELAPOR1 functions as a tethering factor that regulates PAV fusion through a copper-dependent mechanism.
History
DepositionJul 5, 2025-
Header (metadata) releaseJul 23, 2025-
Map releaseJul 23, 2025-
UpdateAug 13, 2025-
Current statusAug 13, 2025Processing site: PDBc / Status: Released

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Structure visualization

Downloads & links

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Map

FileDownload / File: emd_65259.map.gz / Format: CCP4 / Size: 103 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Voxel sizeX=Y=Z: 1.087 Å
Density
Contour LevelBy AUTHOR: 0.2
Minimum - Maximum-2.4240885 - 3.317913
Average (Standard dev.)-0.0015328772 (±0.05691853)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions300300300
Spacing300300300
CellA=B=C: 326.1 Å
α=β=γ: 90.0 °

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Supplemental data

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Sample components

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Entire : Elapor1 mutant

EntireName: Elapor1 mutant
Components
  • Complex: Elapor1 mutant
    • Protein or peptide: Endosome/lysosome-associated apoptosis and autophagy regulator 1
  • Ligand: 2-acetamido-2-deoxy-beta-D-glucopyranose

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Supramolecule #1: Elapor1 mutant

SupramoleculeName: Elapor1 mutant / type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1
Source (natural)Organism: Mus musculus (house mouse)

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Macromolecule #1: Endosome/lysosome-associated apoptosis and autophagy regulator 1

MacromoleculeName: Endosome/lysosome-associated apoptosis and autophagy regulator 1
type: protein_or_peptide / ID: 1 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Mus musculus (house mouse)
Molecular weightTheoretical: 110.429422 KDa
Recombinant expressionOrganism: Homo sapiens (human)
SequenceString: MKTIIALSYI FCLVFADYKD DDDKGSHHHH HHSGMGPELA ACKESEYAFE YTACDSTGSR WRVAVPASPG LCTSLPDPVK GTECSFSCN AGEFLDMKDQ SCKPCAEGRY SLGTGIRFDE WDELPHGFAS LSANLEVDDS ISESTENCTS SKWVPRGDYI A SNTDECTA ...String:
MKTIIALSYI FCLVFADYKD DDDKGSHHHH HHSGMGPELA ACKESEYAFE YTACDSTGSR WRVAVPASPG LCTSLPDPVK GTECSFSCN AGEFLDMKDQ SCKPCAEGRY SLGTGIRFDE WDELPHGFAS LSANLEVDDS ISESTENCTS SKWVPRGDYI A SNTDECTA TLMYAVNLKQ SGTVNFEYYY PDSSIIFEFF VQNDQCQPSA DDSRWMKTTE KGWEFHSVEL NRGNNVLYWR TT AFSVWSK VSKPVLVRNI AITGVAYTSE CFPCKPGTYA AKQGSPFCKL CPANYYSNKG ETSCAPCDAD KYSEKGSSTC KVR PACTDK DYFYTHTACD AQGETQLMYK WAIPKICGED LEGAVKLPAS GVKTRCPPCN PGFFKTDNST CEPCPYGSYS NGSD CTHCP AGTEPAVGFE YKWWNTLPSN METTVLSGIN FEYKGLTGWE VAGDHIYTAV GASDNDFMIL TLVVPGFRPP QSVVA DTEN KEVARITFVF ETICSVNCEL YFMVGMNSRT NTPVETWKGT KGKQSYTYTI EENATVSFTW AFQRTTLHET GRKYTN DVA KIYSINVTNV MGGVASYCRP CALEASDLGS SCTSCPAGHY INRDSGTCHL CPSNTILKAH QPYGAQACVP CGPGTKN NK IHSLCYNDCT FSRNTPSRIF NYNFSALAGT VSLAGVPSFT SKGLKYFHHF TLSLCGNQGK KMAVCTDNVT DLRIPDGE A GFSKSVTAYV CQVVIIPSEV MGYKAGVSSQ PVSLADRLVG VSTDMTLEGI VSPVELFHPE TSGIPDIVFF FRSNDVTQS CSSGRSTTIR LRCNPMKAAP GTLRLPSMCS DGTCDGCNFH FLWESVAACP LCSASDYHTF VSSCVAGIQK TTYMWREPKL CSGGISLPE QRVTICKTID FWLKVGISAG TCTAILLTVL TCYFWKKNQK LEYKYSKLVM NATLKDCDLP AADSCAIMEG E DVEDDFIF TSKKSLFGKI KSFTSKRTPD GFDSVPLKTS SGGPDMDM

UniProtKB: Endosome/lysosome-associated apoptosis and autophagy regulator 1

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Macromolecule #3: 2-acetamido-2-deoxy-beta-D-glucopyranose

MacromoleculeName: 2-acetamido-2-deoxy-beta-D-glucopyranose / type: ligand / ID: 3 / Number of copies: 6 / Formula: NAG
Molecular weightTheoretical: 221.208 Da
Chemical component information

ChemComp-NAG:
2-acetamido-2-deoxy-beta-D-glucopyranose

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

BufferpH: 7.4
VitrificationCryogen name: ETHANE

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Electron microscopy

MicroscopeFEI TALOS ARCTICA
Image recordingFilm or detector model: GATAN K3 (6k x 4k) / Average electron dose: 50.0 e/Å2
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 1.8 µm / Nominal defocus min: 1.6 µm
Experimental equipment
Model: Talos Arctica / Image courtesy: FEI Company

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Image processing

CTF correctionSoftware - Name: cryoSPARC / Type: PHASE FLIPPING AND AMPLITUDE CORRECTION
Startup modelType of model: INSILICO MODEL / In silico model: alphafold predicted model
Final reconstructionResolution.type: BY AUTHOR / Resolution: 3.6 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: Coot / Number images used: 196230
Initial angle assignmentType: MAXIMUM LIKELIHOOD / Software - Name: cryoSPARC
Final angle assignmentType: MAXIMUM LIKELIHOOD / Software - Name: cryoSPARC

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