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- EMDB-64312: TRiC_HDAC1_close_state -

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ID or keywords:

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Basic information

Entry
Database: EMDB / ID: EMD-64312
TitleTRiC_HDAC1_close_state
Map data
Sample
  • Complex: TRiC complex eith HDAC3
    • Protein or peptide: x 8 types
  • Ligand: x 4 types
KeywordsTRiC complex / CHAPERONE
Function / homology
Function and homology information


positive regulation of protein localization to Cajal body / zona pellucida receptor complex / positive regulation of establishment of protein localization to telomere / scaRNA localization to Cajal body / positive regulation of telomerase RNA localization to Cajal body / chaperonin-containing T-complex / BBSome-mediated cargo-targeting to cilium / tubulin complex assembly / Formation of tubulin folding intermediates by CCT/TriC / binding of sperm to zona pellucida ...positive regulation of protein localization to Cajal body / zona pellucida receptor complex / positive regulation of establishment of protein localization to telomere / scaRNA localization to Cajal body / positive regulation of telomerase RNA localization to Cajal body / chaperonin-containing T-complex / BBSome-mediated cargo-targeting to cilium / tubulin complex assembly / Formation of tubulin folding intermediates by CCT/TriC / binding of sperm to zona pellucida / Folding of actin by CCT/TriC / Prefoldin mediated transfer of substrate to CCT/TriC / RHOBTB1 GTPase cycle / WD40-repeat domain binding / pericentriolar material / Association of TriC/CCT with target proteins during biosynthesis / sperm head-tail coupling apparatus / chaperone-mediated protein complex assembly / RHOBTB2 GTPase cycle / Hydrolases; Acting on acid anhydrides; In phosphorus-containing anhydrides / beta-tubulin binding / heterochromatin / positive regulation of telomere maintenance via telomerase / protein folding chaperone / Gene and protein expression by JAK-STAT signaling after Interleukin-12 stimulation / acrosomal vesicle / mRNA 3'-UTR binding / ATP-dependent protein folding chaperone / mRNA 5'-UTR binding / response to virus / azurophil granule lumen / : / melanosome / Cooperation of PDCL (PhLP1) and TRiC/CCT in G-protein beta folding / G-protein beta-subunit binding / protein folding / cell body / sperm midpiece / secretory granule lumen / ficolin-1-rich granule lumen / cytoskeleton / microtubule / protein stabilization / cadherin binding / Neutrophil degranulation / ubiquitin protein ligase binding / centrosome / Golgi apparatus / ATP hydrolysis activity / RNA binding / extracellular exosome / extracellular region / nucleoplasm / ATP binding / cytoplasm / cytosol
Similarity search - Function
T-complex protein 1, alpha subunit / T-complex protein 1, eta subunit / T-complex protein 1, theta subunit / T-complex protein 1, zeta subunit / T-complex protein 1, delta subunit / T-complex protein 1, gamma subunit / T-complex protein 1, epsilon subunit / T-complex protein 1, beta subunit / : / Chaperonins TCP-1 signature 1. ...T-complex protein 1, alpha subunit / T-complex protein 1, eta subunit / T-complex protein 1, theta subunit / T-complex protein 1, zeta subunit / T-complex protein 1, delta subunit / T-complex protein 1, gamma subunit / T-complex protein 1, epsilon subunit / T-complex protein 1, beta subunit / : / Chaperonins TCP-1 signature 1. / : / Chaperonins TCP-1 signature 2. / Chaperonin TCP-1, conserved site / Chaperonins TCP-1 signature 3. / Chaperone tailless complex polypeptide 1 (TCP-1) / GroEL-like equatorial domain superfamily / TCP-1-like chaperonin intermediate domain superfamily / GroEL-like apical domain superfamily / TCP-1/cpn60 chaperonin family / Chaperonin Cpn60/GroEL/TCP-1 family
Similarity search - Domain/homology
T-complex protein 1 subunit alpha / T-complex protein 1 subunit zeta / T-complex protein 1 subunit epsilon / T-complex protein 1 subunit gamma / T-complex protein 1 subunit theta / T-complex protein 1 subunit delta / T-complex protein 1 subunit beta / T-complex protein 1 subunit eta
Similarity search - Component
Biological speciesHomo sapiens (human)
Methodsingle particle reconstruction / cryo EM / Resolution: 3.64 Å
AuthorsLi ZL / Cong YC
Funding support China, 1 items
OrganizationGrant numberCountry
National Natural Science Foundation of China (NSFC) China
CitationJournal: To Be Published
Title: TRiC-assisted folding of class I HDAC family proteins regulated by distinct co-chaperone and cofactor networks
Authors: Li ZL / Cong YC
History
DepositionApr 23, 2025-
Header (metadata) releaseApr 29, 2026-
Map releaseApr 29, 2026-
UpdateApr 29, 2026-
Current statusApr 29, 2026Processing site: PDBc / Status: Released

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Structure visualization

Supplemental images

emd_64312.png

Downloads & links

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Map

FileDownload / File: emd_64312.map.gz / Format: CCP4 / Size: 244.1 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Projections & slices

Image control

Size
Brightness
Contrast
Others
AxesZ (Sec.)Y (Row.)X (Col.)
0.87 Å/pix.
x 400 pix.
= 348. Å		0.87 Å/pix.
x 400 pix.
= 348. Å		0.87 Å/pix.
x 400 pix.
= 348. Å

Surface

Projections

Slices (1/3)

Slices (1/2)

Slices (2/3)

Images are generated by Spider.

Voxel sizeX=Y=Z: 0.87 Å
Density

Histogram

Histogram (log scale)
Contour LevelBy AUTHOR: 2.5
Minimum - Maximum-0.12591843 - 15.577579
Average (Standard dev.)0.016344974 (±0.5882186)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions400400400
Spacing400400400
CellA=B=C: 348.0 Å
α=β=γ: 90.0 °

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Supplemental data

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Mask #1

Fileemd_64312_msk_1.map
Projections & Slices
AxesZYX

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Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Additional map: #1

Fileemd_64312_additional_1.map
Projections & Slices
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Histogram

Histogram (log scale)

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Half map: #2

Fileemd_64312_half_map_1.map
Projections & Slices
AxesZYX

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Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Half map: #1

Fileemd_64312_half_map_2.map
Projections & Slices
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Histogram

Histogram (log scale)

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Sample components

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Entire : TRiC complex eith HDAC3

EntireName: TRiC complex eith HDAC3
Components
  • Complex: TRiC complex eith HDAC3
    • Protein or peptide: T-complex protein 1 subunit alpha
    • Protein or peptide: T-complex protein 1 subunit beta
    • Protein or peptide: T-complex protein 1 subunit gamma
    • Protein or peptide: T-complex protein 1 subunit delta
    • Protein or peptide: T-complex protein 1 subunit epsilon
    • Protein or peptide: T-complex protein 1 subunit zeta
    • Protein or peptide: T-complex protein 1 subunit eta
    • Protein or peptide: T-complex protein 1 subunit theta
  • Ligand: ADENOSINE-5'-DIPHOSPHATE
  • Ligand: MAGNESIUM ION
  • Ligand: ALUMINUM FLUORIDE
  • Ligand: water

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Supramolecule #1: TRiC complex eith HDAC3

SupramoleculeName: TRiC complex eith HDAC3 / type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#8
Source (natural)Organism: Homo sapiens (human)

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Macromolecule #1: T-complex protein 1 subunit alpha

MacromoleculeName: T-complex protein 1 subunit alpha / type: protein_or_peptide / ID: 1 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 57.840812 KDa
Recombinant expressionOrganism: Homo sapiens (human)
SequenceString: PLSVFGDRST GETIRSQNVM AAASIANIVK SSLGPVGLDK MLVDDIGDVT ITNDGATILK LLEVEHPAAK VLCELADLQD KEVGDGTTS VVIIAAELLK NADELVKQKI HPTSVISGYR LACKEAVRYI NENLIVNTDE LGRDCLINAA KTSMSSKIIG I NGDFFANM ...String:
PLSVFGDRST GETIRSQNVM AAASIANIVK SSLGPVGLDK MLVDDIGDVT ITNDGATILK LLEVEHPAAK VLCELADLQD KEVGDGTTS VVIIAAELLK NADELVKQKI HPTSVISGYR LACKEAVRYI NENLIVNTDE LGRDCLINAA KTSMSSKIIG I NGDFFANM VVDAVLAIKY TDIRGQPRYP VNSVNILKAH GRSQMESMLI SGYALNCVVG SQGMPKRIVN AKIACLDFSL QK TKMKLGV QVVITDPEKL DQIRQRESDI TKERIQKILA TGANVILTTG GIDDMCLKYF VEAGAMAVRR VLKRDLKRIA KAS GATILS TLANLEGEET FEAAMLGQAE EVVQERICDD ELILIKNTKA RTSASIILRG ANDFMCDEME RSLHDALCVV KRVL ESKSV VPGGGAVEAA LSIYLENYAT SMGSREQLAI AEFARSLLVI PNTLAVNAAQ DSTDLVAKLR AFHNEAQVNP ERKNL KWIG LDLSNGKPRD NKQAGVFEPT IVKVKSLKFA TEAAITILRI DDLIKLHP

UniProtKB: T-complex protein 1 subunit alpha

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Macromolecule #2: T-complex protein 1 subunit beta

MacromoleculeName: T-complex protein 1 subunit beta / type: protein_or_peptide / ID: 2 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 56.490855 KDa
Recombinant expressionOrganism: Homo sapiens (human)
SequenceString: ASLSLAPVNI FKAGADEERA ETARLTSFIG AIAIGDLVKS TLGPKGMDKI LLSSGRDASL MVTNDGATIL KNIGVDNPAA KVLVDMSRV QDDEVGDGTT SVTVLAAELL REAESLIAKK IHPQTIIAGW REATKAAREA LLSSAVDHGS DEVKFRQDLM N IAGTTLSS ...String:
ASLSLAPVNI FKAGADEERA ETARLTSFIG AIAIGDLVKS TLGPKGMDKI LLSSGRDASL MVTNDGATIL KNIGVDNPAA KVLVDMSRV QDDEVGDGTT SVTVLAAELL REAESLIAKK IHPQTIIAGW REATKAAREA LLSSAVDHGS DEVKFRQDLM N IAGTTLSS KLLTHHKDHF TKLAVEAVLR LKGSGNLEAI HIIKKLGGSL ADSYLDEGFL LDKKIGVNQP KRIENAKILI AN TGMDTDK IKIFGSRVRV DSTAKVAEIE HAEKEKMKEK VERILKHGIN CFINRQLIYN YPEQLFGAAG VMAIEHADFA GVE RLALVT GGEIASTFDH PELVKLGSCK LIEEVMIGED KLIHFSGVAL GEACTIVLRG ATQQILDEAE RSLHDALCVL AQTV KDSRT VYGGGCSEML MAHAVTQLAN RTPGKEAVAM ESYAKALRML PTIIADNAGY DSADLVAQLR AAHSEGNTTA GLDMR EGTI GDMAILGITE SFQVKRQVLL SAAEAAEVIL RVDNIIKAAP RK

UniProtKB: T-complex protein 1 subunit beta

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Macromolecule #3: T-complex protein 1 subunit gamma

MacromoleculeName: T-complex protein 1 subunit gamma / type: protein_or_peptide / ID: 3 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 58.585738 KDa
Recombinant expressionOrganism: Homo sapiens (human)
SequenceString: RPVLVLSQNT KRESGRKVQS GNINAAKTIA DIIRTCLGPK SMMKMLLDPM GGIVMTNDGN AILREIQVQH PAAKSMIEIS RTQDEEVGD GTTSVIILAG EMLSVAEHFL EQQMHPTVVI SAYRKALDDM ISTLKKISIP VDISDSDMML NIINSSITTK A ISRWSSLA ...String:
RPVLVLSQNT KRESGRKVQS GNINAAKTIA DIIRTCLGPK SMMKMLLDPM GGIVMTNDGN AILREIQVQH PAAKSMIEIS RTQDEEVGD GTTSVIILAG EMLSVAEHFL EQQMHPTVVI SAYRKALDDM ISTLKKISIP VDISDSDMML NIINSSITTK A ISRWSSLA CNIALDAVKM VQFEENGRKE IDIKKYARVE KIPGGIIEDS CVLRGVMINK DVTHPRMRRY IKNPRIVLLD SS LEYKKGE SQTDIEITRE EDFTRILQME EEYIQQLCED IIQLKPDVVI TEKGISDLAQ HYLMRANITA IRRVRKTDNN RIA RACGAR IVSRPEELRE DDVGTGAGLL EIKKIGDEYF TFITDCKDPK ACTILLRGAS KEILSEVERN LQDAMQVCRN VLLD PQLVP GGGASEMAVA HALTEKSKAM TGVEQWPYRA VAQALEVIPR TLIQNCGAST IRLLTSLRAK HTQENCETWG VNGET GTLV DMKELGIWEP LAVKLQTYKT AVETAVLLLR IDDIVSGHKK K

UniProtKB: T-complex protein 1 subunit gamma

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Macromolecule #4: T-complex protein 1 subunit delta

MacromoleculeName: T-complex protein 1 subunit delta / type: protein_or_peptide / ID: 4 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 55.998875 KDa
Recombinant expressionOrganism: Homo sapiens (human)
SequenceString: AYQDRDKPAQ IRFSNISAAK AVADAIRTSL GPKGMDKMIQ DGKGDVTITN DGATILKQMQ VLHPAARMLV ELSKAQDIEA GDGTTSVVI IAGSLLDSCT KLLQKGIHPT IISESFQKAL EKGIEILTDM SRPVELSDRE TLLNSATTSL NSKVVSQYSS L LSPMSVNA ...String:
AYQDRDKPAQ IRFSNISAAK AVADAIRTSL GPKGMDKMIQ DGKGDVTITN DGATILKQMQ VLHPAARMLV ELSKAQDIEA GDGTTSVVI IAGSLLDSCT KLLQKGIHPT IISESFQKAL EKGIEILTDM SRPVELSDRE TLLNSATTSL NSKVVSQYSS L LSPMSVNA VMKVIDPATA TSVDLRDIKI VKKLGGTIDD CELVEGLVLT QKVSNSGITR VEKAKIGLIQ FCLSAPKTDM DN QIVVSDY AQMDRVLREE RAYILNLVKQ IKKTGCNVLL IQKSILRDAL SDLALHFLNK MKIMVIKDIE REDIEFICKT IGT KPVAHI DQFTADMLGS AELAEEVNLN GSGKLLKITG CASPGKTVTI VVRGSNKLVI EEAERSIHDA LCVIRCLVKK RALI AGGGA PEIELALRLT EYSRTLSGME SYCVRAFADA MEVIPSTLAE NAGLNPISTV TELRNRHAQG EKTAGINVRK GGISN ILEE LVVQPLLVSV SALTLATETV RSILKIDDVV NTR

UniProtKB: T-complex protein 1 subunit delta

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Macromolecule #5: T-complex protein 1 subunit epsilon

MacromoleculeName: T-complex protein 1 subunit epsilon / type: protein_or_peptide / ID: 5 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 58.696828 KDa
Recombinant expressionOrganism: Homo sapiens (human)
SequenceString: LAFDEYGRPF LIIKDQDRKS RLMGLEALKS HIMAAKAVAN TMRTSLGPNG LDKMMVDKDG DVTVTNDGAT ILSMMDVDHQ IAKLMVELS KSQDDEIGDG TTGVVVLAGA LLEEAEQLLD RGIHPIRIAD GYEQAARVAI EHLDKISDSV LVDIKDTEPL I QTAKTTLG ...String:
LAFDEYGRPF LIIKDQDRKS RLMGLEALKS HIMAAKAVAN TMRTSLGPNG LDKMMVDKDG DVTVTNDGAT ILSMMDVDHQ IAKLMVELS KSQDDEIGDG TTGVVVLAGA LLEEAEQLLD RGIHPIRIAD GYEQAARVAI EHLDKISDSV LVDIKDTEPL I QTAKTTLG SKVVNSCHRQ MAEIAVNAVL TVADMERRDV DFELIKVEGK VGGRLEDTKL IKGVIVDKDF SHPQMPKKVE DA KIAILTC PFEPPKPKTK HKLDVTSVED YKALQKYEKE KFEEMIQQIK ETGANLAICQ WGFDDEANHL LLQNNLPAVR WVG GPEIEL IAIATGGRIV PRFSELTAEK LGFAGLVQEI SFGTTKDKML VIEQCKNSRA VTIFIRGGNK MIIEEAKRSL HDAL CVIRN LIRDNRVVYG GGAAEISCAL AVSQEADKCP TLEQYAMRAF ADALEVIPMA LSENSGMNPI QTMTEVRARQ VKEMN PALG IDCLHKGTND MKQQHVIETL IGKKQQISLA TQMVRMILKI DDIRKPG

UniProtKB: T-complex protein 1 subunit epsilon

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Macromolecule #6: T-complex protein 1 subunit zeta

MacromoleculeName: T-complex protein 1 subunit zeta / type: protein_or_peptide / ID: 6 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 57.50134 KDa
Recombinant expressionOrganism: Homo sapiens (human)
SequenceString: AAVKTLNPKA EVARAQAALA VNISAARGLQ DVLRTNLGPK GTMKMLVSGA GDIKLTKDGN VLLHEMQIQH PTASLIAKVA TAQDDITGD GTTSNVLIIG ELLKQADLYI SEGLHPRIIT EGFEAAKEKA LQFLEEVKVS REMDRETLID VARTSLRTKV H AELADVLT ...String:
AAVKTLNPKA EVARAQAALA VNISAARGLQ DVLRTNLGPK GTMKMLVSGA GDIKLTKDGN VLLHEMQIQH PTASLIAKVA TAQDDITGD GTTSNVLIIG ELLKQADLYI SEGLHPRIIT EGFEAAKEKA LQFLEEVKVS REMDRETLID VARTSLRTKV H AELADVLT EAVVDSILAI KKQDEPIDLF MIEIMEMKHK SETDTSLIRG LVLDHGARHP DMKKRVEDAY ILTCNVSLEY EK TEVNSGF FYKSAEEREK LVKAERKFIE DRVKKIIELK RKVCGDSDKG FVVINQKGID PFSLDALSKE GIVALRRAKR RNM ERLTLA CGGVALNSFD DLSPDCLGHA GLVYEYTLGE EKFTFIEKCN NPRSVTLLIK GPNKHTLTQI KDAVRDGLRA VKNA IDDGC VVPGAGAVEV AMAEALIKHK PSVKGRAQLG VQAFADALLI IPKVLAQNSG FDLQETLVKI QAEHSESGQL VGVDL NTGE PMVAAEVGVW DNYCVKKQLL HSCTVIATNI LLVDEIMRAG M

UniProtKB: T-complex protein 1 subunit zeta

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Macromolecule #7: T-complex protein 1 subunit eta

MacromoleculeName: T-complex protein 1 subunit eta / type: protein_or_peptide / ID: 7 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 57.336027 KDa
Recombinant expressionOrganism: Homo sapiens (human)
SequenceString: PTPVILLKEG TDSSQGIPQL VSNISACQVI AEAVRTTLGP RGMDKLIVDG RGKATISNDG ATILKLLDVV HPAAKTLVDI AKSQDAEVG DGTTSVTLLA AEFLKQVKPY VEEGLHPQII IRAFRTATQL AVNKIKEIAV TVKKADKVEQ RKLLEKCAMT A LSSKLISQ ...String:
PTPVILLKEG TDSSQGIPQL VSNISACQVI AEAVRTTLGP RGMDKLIVDG RGKATISNDG ATILKLLDVV HPAAKTLVDI AKSQDAEVG DGTTSVTLLA AEFLKQVKPY VEEGLHPQII IRAFRTATQL AVNKIKEIAV TVKKADKVEQ RKLLEKCAMT A LSSKLISQ QKAFFAKMVV DAVMMLDDLL QLKMIGIKKV QGGALEDSQL VAGVAFKKTF SYAGFEMQPK KYHNPKIALL NV ELELKAE KDNAEIRVHT VEDYQAIVDA EWNILYDKLE KIHHSGAKVV SSKLPIGDVA TQYFADRDMF CAGRVPEEDL KRT MMACGG SIQTSVNALS ADVLGRCQVF EETQIGGERY NFFTGCPKAK TCTFILRGGA EQFMEETERS LHDAIMIVRR AIKN DSVVA GGGAIEMELS KYLRDYSRTI PGKQQLLIGA YAKALEIIPR QLCDNAGFDA TNILNKLRAR HAQGGTWYGV DINNE DIAD NFEAFVWEPA MVRINALTAA SEAACLIVSV DETIKNPRS

UniProtKB: T-complex protein 1 subunit eta

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Macromolecule #8: T-complex protein 1 subunit theta

MacromoleculeName: T-complex protein 1 subunit theta / type: protein_or_peptide / ID: 8 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 57.619207 KDa
Recombinant expressionOrganism: Homo sapiens (human)
SequenceString: ALHVPKAPGF AQMLKEGAKH FSGLEEAVYR NIQACKELAQ TTRTAYGPNG MNKMVINHLE KLFVTNDAAT ILRELEVQHP AAKMIVMAS HMQEQEVGDG TNFVLVFAGA LLELAEELLR IGLSVSEVIE GYEIACRKAH EILPNLVCCS AKNLRDIDEV S SLLRTSIM ...String:
ALHVPKAPGF AQMLKEGAKH FSGLEEAVYR NIQACKELAQ TTRTAYGPNG MNKMVINHLE KLFVTNDAAT ILRELEVQHP AAKMIVMAS HMQEQEVGDG TNFVLVFAGA LLELAEELLR IGLSVSEVIE GYEIACRKAH EILPNLVCCS AKNLRDIDEV S SLLRTSIM SKQYGNEVFL AKLIAQACVS IFPDSGHFNV DNIRVCKILG SGISSSSVLH GMVFKKETEG DVTSVKDAKI AV YSCPFDG MITETKGTVL IKTAEELMNF SKGEENLMDA QVKAIADTGA NVVVTGGKVA DMALHYANKY NIMLVRLNSK WDL RRLCKT VGATALPRLT PPVLEEMGHC DSVYLSEVGD TQVVVFKHEK EDGAISTIVL RGSTDNLMDD IERAVDDGVN TFKV LTRDK RLVPGGGATE IELAKQITSY GETCPGLEQY AIKKFAEAFE AIPRALAENS GVKANEVISK LYAVHQEGNK NVGLD IEAE VPAVKDMLEA GILDTYLGKY WAIKLATNAA VTVLRVDQII MAKPA

UniProtKB: T-complex protein 1 subunit theta

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Macromolecule #9: ADENOSINE-5'-DIPHOSPHATE

MacromoleculeName: ADENOSINE-5'-DIPHOSPHATE / type: ligand / ID: 9 / Number of copies: 8 / Formula: ADP
Molecular weightTheoretical: 427.201 Da
Chemical component information

ChemComp-ADP:
ADENOSINE-5'-DIPHOSPHATE / ADP, energy-carrying molecule*YM

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Macromolecule #10: MAGNESIUM ION

MacromoleculeName: MAGNESIUM ION / type: ligand / ID: 10 / Number of copies: 8 / Formula: MG
Molecular weightTheoretical: 24.305 Da

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Macromolecule #11: ALUMINUM FLUORIDE

MacromoleculeName: ALUMINUM FLUORIDE / type: ligand / ID: 11 / Number of copies: 8 / Formula: AF3
Molecular weightTheoretical: 83.977 Da
Chemical component information

ChemComp-AF3:
ALUMINUM FLUORIDE

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Macromolecule #12: water

MacromoleculeName: water / type: ligand / ID: 12 / Number of copies: 8 / Formula: HOH
Molecular weightTheoretical: 18.015 Da
Chemical component information

ChemComp-HOH:
WATER

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

BufferpH: 7.5
VitrificationCryogen name: ETHANE

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Electron microscopy

MicroscopeTFS KRIOS
Image recordingFilm or detector model: GATAN K3 BIOQUANTUM (6k x 4k) / Average electron dose: 50.0 e/Å2
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 25.0 µm / Nominal defocus min: 8.0 µm
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

CTF correctionType: PHASE FLIPPING AND AMPLITUDE CORRECTION
Startup modelType of model: NONE
Final reconstructionResolution.type: BY AUTHOR / Resolution: 3.64 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 130389
Initial angle assignmentType: RANDOM ASSIGNMENT
Final angle assignmentType: MAXIMUM LIKELIHOOD

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