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- EMDB-62842: Cryo-EM structure of the thermophile spliceosome (state B*Q1) -

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Basic information

Entry
Database: EMDB / ID: EMD-62842
TitleCryo-EM structure of the thermophile spliceosome (state B*Q1)
Map data
Sample
  • Complex: Spliceosome in state B*Q1
    • RNA: x 4 types
    • Protein or peptide: x 34 types
  • Ligand: x 4 types
KeywordsSpilceosome / DHX15 / DHX35 / GPATCH1 / SPLICING/RNA / SPLICING-RNA complex
Function / homology
Function and homology information


Delta14-sterol reductase activity / U2-type post-mRNA release spliceosomal complex / spliceosomal snRNP complex / spliceosomal complex disassembly / ergosterol biosynthetic process / generation of catalytic spliceosome for first transesterification step / nuclear lumen / pICln-Sm protein complex / snRNP binding / SMN-Sm protein complex ...Delta14-sterol reductase activity / U2-type post-mRNA release spliceosomal complex / spliceosomal snRNP complex / spliceosomal complex disassembly / ergosterol biosynthetic process / generation of catalytic spliceosome for first transesterification step / nuclear lumen / pICln-Sm protein complex / snRNP binding / SMN-Sm protein complex / spliceosomal tri-snRNP complex / post-mRNA release spliceosomal complex / U2-type spliceosomal complex / pre-mRNA binding / U2-type catalytic step 2 spliceosome / U1 snRNP / U2 snRNP / U4 snRNP / U2-type prespliceosome / U2-type catalytic step 1 spliceosome / generation of catalytic spliceosome for second transesterification step / spliceosomal complex assembly / precatalytic spliceosome / Prp19 complex / U5 snRNP / U6 snRNA binding / mRNA cis splicing, via spliceosome / U4/U6 x U5 tri-snRNP complex / spliceosomal snRNP assembly / catalytic step 2 spliceosome / protein K63-linked ubiquitination / helicase activity / mRNA processing / spliceosomal complex / peptidylprolyl isomerase / peptidyl-prolyl cis-trans isomerase activity / mRNA splicing, via spliceosome / RING-type E3 ubiquitin transferase / regulation of gene expression / ubiquitin protein ligase activity / protein folding / RNA helicase activity / RNA helicase / mRNA binding / endoplasmic reticulum membrane / metal ion binding / RNA binding / ATP binding / nucleus / cytosol / cytoplasm
Similarity search - Function
Sterol biosynthesis ERG24/DHCR-like / Sterol reductase, conserved site / Ergosterol biosynthesis ERG4/ERG24 family / Sterol reductase family signature 1. / Sterol reductase family signature 2. / : / Nineteen complex-related protein 2 / mRNA splicing factor Cwf18-like / cwf18 pre-mRNA splicing factor / : ...Sterol biosynthesis ERG24/DHCR-like / Sterol reductase, conserved site / Ergosterol biosynthesis ERG4/ERG24 family / Sterol reductase family signature 1. / Sterol reductase family signature 2. / : / Nineteen complex-related protein 2 / mRNA splicing factor Cwf18-like / cwf18 pre-mRNA splicing factor / : / PRP39 C-terminal HAT repeat / cwf21 / Pre-mRNA-splicing factor Isy1 / Slt11, RNA recognition motif / Pre-mRNA-splicing factor Isy1 superfamily / Isy1-like splicing family / Pre-mRNA-splicing factor Cwc2, RNA recognition motif / Torus domain / Torus domain / : / : / Intron-binding protein aquarius, beta-barrel / Intron-binding protein aquarius insert domain / Pre-mRNA-splicing factor SPF27 / Breast carcinoma amplified sequence 2 (BCAS2) / mRNA splicing factor SYF2 / SYF2 splicing factor / CWF11 family / Intron-binding protein aquarius, N-terminal / Intron-binding protein aquarius N-terminal / Helix hairpin bin domain superfamily / : / : / cwf21 domain / mRNA splicing factor Cwf21 domain / Pre-mRNA-processing factor 17 / : / Pre-mRNA-splicing factor SYF1 middle HAT repeat / Pre-mRNA-splicing factor 19 / Pre-mRNA-processing factor 19 / : / : / Prp19/Pso4-like / BUD31/G10-related, conserved site / G10 protein signature 1. / : / STL11, N-terminal / Cyclophilin-type peptidyl-prolyl cis-trans isomerase, cyclophilin A-like / Myb-like domain profile. / : / Pre-mRNA-splicing factor Syf1/CRNKL1 C-terminal HAT repeat / Pre-mRNA-splicing factor Cwc2/Slt11 / SKI-interacting protein SKIP, SNW domain / Pre-mRNA-splicing factor Cwf15/Cwc15 / SKI-interacting protein, SKIP / SKIP/SNW domain / Cwf15/Cwc15 cell cycle control protein / PRP39 N-terminal HAT repeat / WD repeat Prp46/PLRG1-like / Pre-mRNA splicing factor component Cdc5p/Cef1, C-terminal / : / pre-mRNA splicing factor component / G10 protein / : / Pre-mRNA-splicing factor BUD31 / Initiation factor eIF-4 gamma, MA3 / : / : / MA3 domain / Pre-mRNA-splicing factor Syf1/CNRKL1 N-terminal HAT repeat / MI domain profile. / Domain in DAP-5, eIF4G, MA-3 and other proteins. / Anaphase-promoting complex subunit 4, WD40 domain / DNA2/NAM7 helicase, helicase domain / AAA domain / MIF4G domain / Myb-like DNA-binding domain / DNA2/NAM7-like helicase / : / Anaphase-promoting complex subunit 4 WD40 domain / Middle domain of eukaryotic initiation factor 4G (eIF4G) / MIF4G-like, type 3 / Small nuclear ribonucleoprotein D1 / Zinc finger, CCCH-type superfamily / U-box domain profile. / Pre-mRNA-splicing factor Syf1-like / Modified RING finger domain / U-box domain / Small nuclear ribonucleoprotein Sm D2 / Small nuclear ribonucleoprotein Sm D3 / Small nuclear ribonucleoprotein G / Small nuclear ribonucleoprotein E / Small nuclear ribonucleoprotein F / : / Nucleolar protein 10-like, N-terminal domain / : / Helicase associated domain (HA2), ratchet-like / DEAD-box helicase, OB fold / Helicase associated domain (HA2), winged-helix / Oligonucleotide/oligosaccharide-binding (OB)-fold
Similarity search - Domain/homology
Sm protein F / Pre-mRNA splicing protein / RNA helicase / Small nuclear ribonucleoprotein Sm D1 / Pre-mRNA splicing protein / Pre-mRNA-splicing factor PRP46 / Pre-mRNA splicing protein / Peptidyl-prolyl cis-trans isomerase / Uncharacterized protein / Delta(14)-sterol reductase ...Sm protein F / Pre-mRNA splicing protein / RNA helicase / Small nuclear ribonucleoprotein Sm D1 / Pre-mRNA splicing protein / Pre-mRNA-splicing factor PRP46 / Pre-mRNA splicing protein / Peptidyl-prolyl cis-trans isomerase / Uncharacterized protein / Delta(14)-sterol reductase / Pre-mRNA splicing protein / Bud site selection protein / Pre-mRNA-splicing factor SYF2 / Pre-mRNA-splicing factor CWC22 / Pre-mRNA-splicing factor SYF1 / Pre-mRNA splicing protein / Pre-mRNA-splicing factor CWC2 / Pre-mRNA splicing protein / Small nuclear ribonucleoprotein E / Uncharacterized protein / Uncharacterized protein / Pre-mRNA-splicing factor SPF27 / Pre-mRNA-processing protein 45 / Pre-mRNA-splicing factor / Pre-mRNA-processing factor 19 / Small nuclear ribonucleoprotein Sm D3 / Sm protein B / Small nuclear ribonucleoprotein G / Nineteen complex-related protein 2-domain-containing protein
Similarity search - Component
Biological speciesChaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719) (fungus)
Methodsingle particle reconstruction / cryo EM / Resolution: 2.9 Å
AuthorsLi Y / Fischer P / Wang M / Yuan R / Meng W / Luehrmann R / Lau B / Hurt E / Cheng J
Funding support1 items
OrganizationGrant numberCountry
Not funded
CitationJournal: Cell Res / Year: 2025
Title: Structural insights into spliceosome fidelity: DHX35-GPATCH1- mediated rejection of aberrant splicing substrates.
Authors: Yi Li / Paulina Fischer / Mengjiao Wang / Qianxing Zhou / Aixia Song / Rui Yuan / Wanyu Meng / Fei Xavier Chen / Reinhard Lührmann / Benjamin Lau / Ed Hurt / Jingdong Cheng /
Abstract: The spliceosome, a highly dynamic macromolecular assembly, catalyzes the precise removal of introns from pre-mRNAs. Recent studies have provided comprehensive structural insights into the step-wise ...The spliceosome, a highly dynamic macromolecular assembly, catalyzes the precise removal of introns from pre-mRNAs. Recent studies have provided comprehensive structural insights into the step-wise assembly, catalytic splicing and final disassembly of the spliceosome. However, the molecular details of how the spliceosome recognizes and rejects suboptimal splicing substrates remained unclear. Here, we show cryo-electron microscopy structures of spliceosomal quality control complexes from a thermophilic eukaryote, Chaetomium thermophilum. The spliceosomes, henceforth termed B*, are stalled at a catalytically activated state but prior to the first splicing reaction due to an aberrant 5' splice site conformation. This state is recognized by G-patch protein GPATCH1, which is docked onto PRP8-EN and -RH domains and has recruited the cognate DHX35 helicase to its U2 snRNA substrate. In B*, DHX35 has dissociated the U2/branch site helix, while the disassembly helicase DHX15 is docked close to its U6 RNA 3'-end substrate. Our work thus provides mechanistic insights into the concerted action of two spliceosomal helicases in maintaining splicing fidelity by priming spliceosomes that are bound to aberrant splice substrates for disassembly.
History
DepositionDec 23, 2024-
Header (metadata) releaseMar 12, 2025-
Map releaseMar 12, 2025-
UpdateApr 16, 2025-
Current statusApr 16, 2025Processing site: PDBc / Status: Released

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Structure visualization

Supplemental images

emd_62842.png

Downloads & links

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Map

FileDownload / File: emd_62842.map.gz / Format: CCP4 / Size: 421.9 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Projections & slices

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AxesZ (Sec.)Y (Row.)X (Col.)
0.93 Å/pix.
x 480 pix.
= 447.36 Å		0.93 Å/pix.
x 480 pix.
= 447.36 Å		0.93 Å/pix.
x 480 pix.
= 447.36 Å

Surface

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Images are generated by Spider.

Voxel sizeX=Y=Z: 0.932 Å
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Contour LevelBy AUTHOR: 0.0035
Minimum - Maximum-0.016442008 - 0.06250503
Average (Standard dev.)0.00014650554 (±0.0018862549)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions480480480
Spacing480480480
CellA=B=C: 447.36 Å
α=β=γ: 90.0 °

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Supplemental data

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Additional map: #1

Fileemd_62842_additional_1.map
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Additional map: #2

Fileemd_62842_additional_2.map
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Additional map: #3

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Additional map: #4

Fileemd_62842_additional_4.map
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Half map: #1

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Sample components

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Entire : Spliceosome in state B*Q1

EntireName: Spliceosome in state B*Q1
Components
  • Complex: Spliceosome in state B*Q1
    • RNA: U2 snRNA
    • RNA: U5 snRNA
    • RNA: U6 snRNA
    • Protein or peptide: PRP8
    • Protein or peptide: Pre-mRNA-splicing factor SYF2
    • Protein or peptide: SNU114
    • Protein or peptide: Anaphase-promoting complex subunit 4-like WD40 domain-containing protein
    • Protein or peptide: CCDC12
    • Protein or peptide: Putative pre-mRNA splicing protein
    • Protein or peptide: Suppressor of forked domain-containing protein
    • Protein or peptide: Putative pre-mRNA splicing protein
    • Protein or peptide: Pre-mRNA-splicing factor SPF27
    • Protein or peptide: Pre-mRNA-processing factor 19
    • Protein or peptide: Putative bud site selection protein
    • Protein or peptide: Peptidyl-prolyl cis-trans isomerase
    • Protein or peptide: Pre-mRNA-splicing factor PRP46
    • Protein or peptide: Putative pre-mRNA splicing protein
    • Protein or peptide: Putative pre-mRNA splicing protein
    • Protein or peptide: Pre-mRNA-processing protein 45
    • Protein or peptide: PRP17
    • Protein or peptide: Putative pre-mRNA splicing protein
    • Protein or peptide: Pre-mRNA-splicing factor
    • Protein or peptide: GPATCH1
    • Protein or peptide: RNA helicase
    • Protein or peptide: Small nuclear ribonucleoprotein E
    • Protein or peptide: Small nuclear ribonucleoprotein G
    • Protein or peptide: Sm protein F
    • Protein or peptide: Delta(14)-sterol reductase
    • Protein or peptide: Small nuclear ribonucleoprotein Sm D1
    • Protein or peptide: Sm protein B
    • Protein or peptide: Small nuclear ribonucleoprotein Sm D3
    • Protein or peptide: Putative pre-mRNA splicing protein
    • Protein or peptide: Putative pre-mRNA splicing protein
    • Protein or peptide: Nineteen complex-related protein 2-domain-containing protein
    • Protein or peptide: GCFC2
    • Protein or peptide: TFIP11
    • RNA: Unknown mRNA
    • Protein or peptide: Putative pre-mRNA splicing protein
  • Ligand: N,N,7-trimethylguanosine 5'-(trihydrogen diphosphate)
  • Ligand: MAGNESIUM ION
  • Ligand: GUANOSINE-5'-TRIPHOSPHATE
  • Ligand: ZINC ION

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Supramolecule #1: Spliceosome in state B*Q1

SupramoleculeName: Spliceosome in state B*Q1 / type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#38
Source (natural)Organism: Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719) (fungus)

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Macromolecule #1: U2 snRNA

MacromoleculeName: U2 snRNA / type: rna / ID: 1 / Number of copies: 1
Source (natural)Organism: Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719) (fungus)
Molecular weightTheoretical: 61.39384 KDa
SequenceString: AGCUCUCUUU GCCUUUUGGC UUAGAUCAAG UGUAGUAUCU GUUCUUUUCA GUUUAAUCUC UGAAACUGCU CUACGGAGCA GAAUCGUGA UUAUACUAAU UUUUGGCCUU CGGCGGACUU CCCUCUGGGC UUGCCCAUGG UCGUCUGCCA CAGUGUCCCU G GUAUUACA ...String:
AGCUCUCUUU GCCUUUUGGC UUAGAUCAAG UGUAGUAUCU GUUCUUUUCA GUUUAAUCUC UGAAACUGCU CUACGGAGCA GAAUCGUGA UUAUACUAAU UUUUGGCCUU CGGCGGACUU CCCUCUGGGC UUGCCCAUGG UCGUCUGCCA CAGUGUCCCU G GUAUUACA CUGCCUCCAG GUGACGCGAC CUUCC

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Macromolecule #2: U5 snRNA

MacromoleculeName: U5 snRNA / type: rna / ID: 2 / Number of copies: 1
Source (natural)Organism: Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719) (fungus)
Molecular weightTheoretical: 36.997824 KDa
SequenceString:
UUGGAGUAGG CCAGCUCAGA CCGAACUCAU UUCCUGCCUU UUACCGGAUG UGACCGUGAG UUGGCCUGAA AUACUCCCUA ACCCAAUCU UUGGAAACUC UCUGGAUAUC CCAGAUU

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Macromolecule #3: U6 snRNA

MacromoleculeName: U6 snRNA / type: rna / ID: 3 / Number of copies: 1
Source (natural)Organism: Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719) (fungus)
Molecular weightTheoretical: 32.443285 KDa
SequenceString:
GCCCUUCGGG GCAUUUGGUC AAUUUGAAAC GAUACAGAGA AGAUUAGCAU GGCCCCUGCA CUAAGGAUGA CACGCUACUC AAAGAGACG CUACCAAUUU UU

GENBANK: GENBANK: CU633895.1

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Macromolecule #37: Unknown mRNA

MacromoleculeName: Unknown mRNA / type: rna / ID: 37 / Number of copies: 1
Source (natural)Organism: Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719) (fungus)
Molecular weightTheoretical: 7.235635 KDa
SequenceString:
(P5P)(P5P)(P5P)(Y5P)(P5P)(Y5P)(Y5P)(P5P)(P5P)(P5P) (P5P)(P5P)(Y5P)(P5P)(Y5P)(P5P) (Y5P)(P5P)(Y5P) (N)(Y5P)(Y5P)(Y5P)(Y5P)(Y5P)

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Macromolecule #4: PRP8

MacromoleculeName: PRP8 / type: protein_or_peptide / ID: 4 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719) (fungus)
Molecular weightTheoretical: 285.131 KDa
SequenceString: MLRPCALAIW QGFAPLQSRE SSSRFSLFKP RPVPGFHQHT TLKGEVAISN CGEIVAPISF GLWRIFANRS RSNAGNTATC LRFRRLLPD GGPPPPPPPP PPPPGPPSDL PPPPPPSSSS LPPPPAIPAP PPPGYRPPSD PHIAKFAQKK KEWLRMQRNR F GEKRKGGF ...String:
MLRPCALAIW QGFAPLQSRE SSSRFSLFKP RPVPGFHQHT TLKGEVAISN CGEIVAPISF GLWRIFANRS RSNAGNTATC LRFRRLLPD GGPPPPPPPP PPPPGPPSDL PPPPPPSSSS LPPPPAIPAP PPPGYRPPSD PHIAKFAQKK KEWLRMQRNR F GEKRKGGF VETQKADMPP EHLRKIVKDI GDVSQKKYTS DKRSYLGALK FMPHAVLKLL ENMPMPWEAA REVKVLYHVN GC LTLVNEI PRVIEPVFFA QWGSMWRTMR KEKSDRRLFK RMRFPPFDDE EPPLSWSENI EDVEPLEPIQ MELDEDEDAA VYE WFYDHQ PLIDTPHVNG PSYKRWNLTL PQMANLYRLS EPLVSDVVDP NYFYLFELKS FLTAKALNVA LPGGPRFEPL YKDI DPNDE DFGEFNAMDR IIFRNPIRTE TRVAYPYLYN SRPRSVHLSW HSYPQIVYTK TEDPELPAFY FDTSINPISS RAVAP KNLT VSHEDELFGK GNIEEPEEEA FVLPAAVEPF FADEELETED TRSAIELWWA PYPFDRRSGR MVRAQDVPLI KHWYLE HCP PKQPVKVRVS YQKLLKTYVL NELHRKRPKS MQKQSLLRTL KQTKFFQQTT IDWVEAGLQV CRQGFNMLNL LIHRKNL TY LHLDYNFNLK PVKTLTTKER KKSRFGNAFH LMREILRLTK LIVDAQVQYR LGNIDAFQLA DGIHYAFNHV GQLTGMYR Y KYKLMHQIRS CKDLKHLIYY RFNTGPVGKG PGCGFWAPSW RVWLFFMRGI IPLLERWLGN LLSRQFEGRH SKGVAKTVT KQRVESHFDL ELRASVMADL MDMMPEGIKQ SKVNTVLQHL SEAWRCWKSN IPWKVPGLPA PIENIILRYV KAKADWWISV AHYNRERIR RGATVDKTVA KKNVGRLTRL WLKAEQERQH NHMKDGPYVS SEEAVAIYTT TVHWLEARKF SPIPFPSVSY K HDTKILIL ALERLREAYS TKGRLNQSQR EELALIEQAY DSPGTTLERI KRFLLTQRAF KEVGIDMNDN YSTINPVYDV EP IEKISDA YLDQYLWYQA DQRHLFPNWV KPSDSEVPPL LVYKWCQGIN NLDKVWDTSN GECNVIIETQ LSKVYEKIEL TLL NSLLRL IMDHNLADYI TAKNNVTLTY KDMSHVNSYG MIRGLQFSAF VFQYYGLILD LLILGPQRAA EIAGPPQSPN DFLQ FQDRD TETRHPIRLY TRYIDKIWVF LRFTAEESRD LIQRFLTEQP DPNFENVIGF KSKKCWPRDS RMRLMRHDVN LGRAV FWDL KNRLPRSVTT IEWDDTFVSV YSKDNPNLLF SMCGFEVRIL PKCRNQNDEF PVKDSVWSLV DNSTKERTAH AFLQVT EED IQKFNNRIRQ ILMSSGSTTF TKIANKWNTA LIALFTYYRE AAVSTVNLLD TIVKCETKIQ TRVKIGLNSK MPSRFPP AV FYTPKELGGL GMLSGSHILI PASDKRWCKQ TDVGITHFRA GMSHDEETLI PNIARYIIPW EAEFIDSQRV WTEYSQKR L EAQQQNRRLT LEDLEDSWDR GLPRINTLFQ KDRSTLSFDK GFRARAEFKI YQLMKSNPFW WTNPRHDGKL WNLNAYRTD VIQALGGVET ILEHTLFKAT GFPSWEGLFW EKSSGFEASM QFKKLTNAQR SGLNQIPNRR FTLWWSPTIN RANVYVGFQV QLDLTGIFL HGKIPTLKIS LIQIFRAHLW QKIHESVVMD LCQVFDQELE ALSIESVQKE TIHPRKSYKM NSSCADIQLF A SHKWNVTR PSLLFDTKDV IEATTTNKFW IDVQLRYGDY DSHDIERYVR AKYLDYTTDS MSLYPSPTGL MIGIDLAYNL YS AYGQYFP GLKTLIQQAM AKIMKANPAL YVLRERIRKG LQLYASESNQ EFLNSQNYSE LFSNQTQLFI DDTNVYRVTI HKT FEGNLT TKPINGAIFI FNPRTGQLFL KIIHTSVWAG QKRLGQLAKW KTAEEVAALI RSLPVEEQPK QLIVTRKGLL DPLE VNLVD FPNISIRASE LQLPFQAAMK VEKLGDMILR ATEPQMVLFN LYDDWLKTIS SYTAFSRLIL ILRALHVNQD KTKLI LRPD KTVITQDHHI WPSLTDEDWI KVEMQLRDLI LNDYGKKNNV NVASLTTSEV RDIILGMEIS APSLQRQQAA EIEKQQ QEQ AQLTAVTTKT QNVHGEEIIV TTTSQFEQQT FASKTEWRTR AIATSNLRTR ANNMYVAPVD NDVDDITYVM PKNILKK FI TIADLRVQVA GFLYGCSPAD NDQVKEIRCI VMVPQIGGNR SVQLPQHLPQ HEMLKGLEPL GLIHTMAGNE LPYMSPAD V TTHAKLVDAH PSWKNQNTLT VTVAFTPGSV SLSAWALTPL GYKWGVENKD PNVDNPQGFT TTMGERRQLL LSDKFKGFF LVPDTGKWNY SFMGSSFSGI EKKPYHVKLD TPLPFYSEQH RPIHFTSFNE LEDIWVDRAD NFA

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Macromolecule #5: Pre-mRNA-splicing factor SYF2

MacromoleculeName: Pre-mRNA-splicing factor SYF2 / type: protein_or_peptide / ID: 5 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719) (fungus)
Molecular weightTheoretical: 36.945891 KDa
SequenceString: MPPAKKRKTE ASQEPRPASE QQEQNGAQEE QPTVQDSTPE VESRSDDKKV SQSPTLATDP SDDTSEQPSE SSTKNAALTA AQERLARFR ALQARAKESS QQNLKEATKE SQRLATDPSQ LTALSRKHAI AAHKLLKAEI EDAGGDFERK RAWDWTVEEA E RWDKRMKK ...String:
MPPAKKRKTE ASQEPRPASE QQEQNGAQEE QPTVQDSTPE VESRSDDKKV SQSPTLATDP SDDTSEQPSE SSTKNAALTA AQERLARFR ALQARAKESS QQNLKEATKE SQRLATDPSQ LTALSRKHAI AAHKLLKAEI EDAGGDFERK RAWDWTVEEA E RWDKRMKK KEAHRDDTAF RDYAREAEKT YKRQIRNMGA PDLEKYMREK LSAIEKAAAA GTLDIIETED GEMIAVDKDG TF FSTANAT DFAQHKPDKA AVDRLVADLR KAEEASLKRR REKLAKSGEE HGDVTYINEK NKQFNAKLAR FYNKYTAEIR DSF ERGTMV

UniProtKB: Pre-mRNA-splicing factor SYF2

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Macromolecule #6: SNU114

MacromoleculeName: SNU114 / type: protein_or_peptide / ID: 6 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719) (fungus)
Molecular weightTheoretical: 113.867617 KDa
SequenceString: MWQRATRLRL SESFGPSTTD SNHTFPDLAA TMDDDLYDEF GNFIGEEAES EEESDHGANA GVYAYDEYVD EAPEEPAEEQ MDIDEEPPS NAVILHEDKQ YYPTAAQVFG EGVETLVQEE DAQPLTQPII APVEQKKFSI QEADLPPVYF DRGFMTDLMN F PEQIRNIA ...String:
MWQRATRLRL SESFGPSTTD SNHTFPDLAA TMDDDLYDEF GNFIGEEAES EEESDHGANA GVYAYDEYVD EAPEEPAEEQ MDIDEEPPS NAVILHEDKQ YYPTAAQVFG EGVETLVQEE DAQPLTQPII APVEQKKFSI QEADLPPVYF DRGFMTDLMN F PEQIRNIA LAGHLHHGKT AFMDMLVLET HAIQERLDKR TGKKRDEQLR YTDVHVIERD RGLSIKAAPM SLVLSSTKGK SH LFNIIDT PGHVDFVDEV AAAFRLVDGV CLVVDVVEGV QVNTEQIIKH AVLEDIPLTL IINKMDRLIL ELKIPPTDAY FKL KHVIEE VNTVIENTIP GRGESKRLSP EKGNVLFACT SMGWCFTLQS FAKMYSESYG GVNVEEFARR LWGDVYFNPK KRTF TRKPI EEGAKRSFVN FVMEPIYKLY SHTISEGPED LKRTLSKLGI FLKPSQYKAD PKVLMKLVCE QFFGPSTAFV DMVIK HIPS PLEAAEKKLE RYYTGPLDTK IAESMKNCDQ NGPLVVHVTK LFNTIDAKSF YAFGRVMSGI ARPGADVRVL GEGYTL DDE EDMVVSRISD VFIAETRYNI PTDGVPAGNF VLLGGVDNSI VKTATIVDKK FDNGEDAYVF KPLSHFTESV LKVAVEP IN PSELPKMLDG IRKINKSYPL ITTKVEESGE HIILGTGELY MDCVLHDLRK LYADMEVRVS DPVVRFCETV QDMSATKC Y AITPNKKNTI TMAAEPLDDG IAQDIESGAV KIKDPPRKVA KFFEEKYGWD KLAARSIWAF GPDEMGPNIL QDDTLPTEV DKKRLATVKE SIRQGFAWAT REGPLCEEPI RNTKFRLIDV SLFPWWWPNY SDRSTCLLFL FLDGLPRLME PMYSVSMTGP QSSVSMVYN ILSRRRGHVL SDGPIAGTPL YRVNGLIPVI DSFGFETDLR INTPGQAMVS LVFDRWSIVP GDPLDREQVT R PLQMATAQ ATARDFVLKT RRRKGLSEDV TVAKFLEPEF YQSLLESGTL GEP

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Macromolecule #7: Anaphase-promoting complex subunit 4-like WD40 domain-containing ...

MacromoleculeName: Anaphase-promoting complex subunit 4-like WD40 domain-containing protein
type: protein_or_peptide / ID: 7 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719) (fungus)
Molecular weightTheoretical: 38.229977 KDa
SequenceString: MSEKRPASDD PGEGQLVVKR QNVGSSRALT RTGDSAGALI QTTPRTSNLK APLMELSGHT GEIFTAKFDP TGTLIASGSM DRTIMLWRV YGDCENYGVL NGHKAAILDL QWSRDGDILF SASADMHLAS WDLTTGQRIR RYIGHEEIIN AMDVTQRGEE L LISGSDDG ...String:
MSEKRPASDD PGEGQLVVKR QNVGSSRALT RTGDSAGALI QTTPRTSNLK APLMELSGHT GEIFTAKFDP TGTLIASGSM DRTIMLWRV YGDCENYGVL NGHKAAILDL QWSRDGDILF SASADMHLAS WDLTTGQRIR RYIGHEEIIN AMDVTQRGEE L LISGSDDG TIGIWDPRTK NAVDYIETDF PITAVAVSEA GNEIYSGGID NDIKVWDIRK KAVVHTMLGH NDTITTLRVS PD GQQLLSY AMDSTARTWD IRPFAPTDRH IRTFDGAPLG LEKNLIRGSW SKDGKKIAVG AGDGTVVIWG SDTGKLLYKL PGH KGTVNC AEFAPDGAPI ILSASSDRTM LLGELI

UniProtKB: Uncharacterized protein

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Macromolecule #8: CCDC12

MacromoleculeName: CCDC12 / type: protein_or_peptide / ID: 8 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719) (fungus)
Molecular weightTheoretical: 25.360002 KDa
SequenceString: MSSYSSLSAA ADERKARLAK LASLKRKQPE DDHPPSQDQQ PEQSAAESKQ EGDSSQASDP VLQHLSLRNY DPVTRGPKLG FEAPPTENL NTLTLEEKAA ALEAEARRKA QEEQEAAAQA RGLDITTLQP KKPNWDLKRE FKQRMAVLDV RTENAIARMV R ERLAEKKK ...String:
MSSYSSLSAA ADERKARLAK LASLKRKQPE DDHPPSQDQQ PEQSAAESKQ EGDSSQASDP VLQHLSLRNY DPVTRGPKLG FEAPPTENL NTLTLEEKAA ALEAEARRKA QEEQEAAAQA RGLDITTLQP KKPNWDLKRE FKQRMAVLDV RTENAIARMV R ERLAEKKK AIVGASAAAS GAADGAASGG GNNGKGEEGV LEGAEILEGM RMREKEEEEE ARREKEAEEA EFGTA

UniProtKB: Uncharacterized protein

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Macromolecule #9: Putative pre-mRNA splicing protein

MacromoleculeName: Putative pre-mRNA splicing protein / type: protein_or_peptide / ID: 9 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719) (fungus)
Molecular weightTheoretical: 97.944164 KDa
SequenceString: MPSTLASDRQ PDVALVCEDD APYEQDILRN PGSIRPWLSY IEYKLQHGTL REQAFVMERA CVQLPRSYKL WKMFRVNHIS KLNPAIFAT EYQKVNALFE RALILLNKMP RIWEMYLKFL MQQPLVTFTR RTFDRALRAL PITQHNRIWA LYRPFANSAE G ITAVKIWR ...String:
MPSTLASDRQ PDVALVCEDD APYEQDILRN PGSIRPWLSY IEYKLQHGTL REQAFVMERA CVQLPRSYKL WKMFRVNHIS KLNPAIFAT EYQKVNALFE RALILLNKMP RIWEMYLKFL MQQPLVTFTR RTFDRALRAL PITQHNRIWA LYRPFANSAE G ITAVKIWR RYMQVHPEDA EDFIELLIQC GLYTEAVKKY IEILNNPKFQ SKNAKGHYEL WSEMVDLLVE HAVDIETGHE TG IDVERII RSGIERFSDQ RGKLWSGLAT YWIRRGNFDR ARDVFEEGIT TVMTVRDFTM IFDAYVEFEE SVIGTLMEAA SRR AEKGVV DESADFDLDI RMMRFEHLMD RRPFLLNDVL LRQNPNNVAE WEKRVALWGD NKEEVVKTYT DAIAAINPKK AVGA FHLLW ANYAKFYEKA GDLRTARIIM EKAVKVPFKS VNELADMWIE WAEMELRNKN FDEAVRIMAK ATQAPKRSTV DYFDE SLSP QQRVHKSWKL WSFYVDLVES TSSLEETRKI YERIFELRIA TPQTVVNYAN LLEEHHYYEE SFKIYERGLD LFSYPV AFE LWNLYLTKAV DRKISIERLR DLFEQAITDC PPKFAKVLYL MYGNLEEERG LARHAMRIYE RATRAVADED RADMFNF YI TKSASNFGLA STRPIYERAI ATLPDNEARD MCLKFADMEK RLGEIDRARA IYGHASQFCD PRTNPEFWAK WEQFEVQH G NEDTFKEMLR VKRSVQAKYN TDVNFIASQA LARSQMLKQQ QQQQQQQQQQ QLQQQQQNGS SGAGDPEVAD AMAQLERQA RAPAGFVAAS ENIKGTIAPS TQSVEVSNPD AIDLDDMDE

UniProtKB: Pre-mRNA-splicing factor SYF1

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Macromolecule #10: Suppressor of forked domain-containing protein

MacromoleculeName: Suppressor of forked domain-containing protein / type: protein_or_peptide / ID: 10 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719) (fungus)
Molecular weightTheoretical: 82.195375 KDa
SequenceString: MESSRGPPRV KNKAPAPIQI SAEQLLREAV DRQETIIHKP TQRFADLEEL KEYQGRKRRE FEDYIRRNRL RLQNWFQYAQ WELEQKEFA RARSIFERAL DVHPNNTQLW IRYIEAELKN RNINHARNLL DRAVTRLPRV SKLWYKYVYV MEMLGDIPGT R QVFDRWMK ...String:
MESSRGPPRV KNKAPAPIQI SAEQLLREAV DRQETIIHKP TQRFADLEEL KEYQGRKRRE FEDYIRRNRL RLQNWFQYAQ WELEQKEFA RARSIFERAL DVHPNNTQLW IRYIEAELKN RNINHARNLL DRAVTRLPRV SKLWYKYVYV MEMLGDIPGT R QVFDRWMK WEPDEDAWNA YIKLEKRYGE YERARQIFAA YTQVHPEPRT WLKWAKFEEE FGTADMVRDV FQSAIQYIAE TL GDDAVDE RLFIAFARFE TRQKEYERAR AIYKFGLDNL PRSRSMQLHA QYTTFEKQFG DKEGVEDVVL TKRRRLYEEQ VKE NPKNYD VWFDFARLEE MGGDPDRVRE VYERAIAQVP PTQEKRHWRR YIFLFLFYAI WEEKDAKNIE RARAIYDTCL NLIP HKKFT FAKVWIAKAH FEIRQGNLTA ARKTLGRAIG MCPKDKLFRE YIAIEQKLYE FDRCRTLYEK HALFNPANCQ TWIRW AELE RGLDDLDRTR AIFEVAISQP VLDMPEVVWK AYIDFEEEEG EYERARALYE RLLQKADHPK VWISYAQFEI NIPDTE TEA QAAEGEEIPV SEAAKARARG VFERALKSMK ERDLKAERVA LLRAWLEFER THGAAEDVER IRRQMPREVK KKRRIDE DT WEEYVDYVFP ADEQQTKNLS NLLAMAKQWK EKTGGGIMGL GAGGS

UniProtKB: Pre-mRNA splicing protein

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Macromolecule #11: Putative pre-mRNA splicing protein

MacromoleculeName: Putative pre-mRNA splicing protein / type: protein_or_peptide / ID: 11 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719) (fungus)
Molecular weightTheoretical: 86.636156 KDa
SequenceString: MPVVKGGVWT NIEDEILKAA VSKYGLNQWA RVSSLLARKT PKQCKARWNE WLDPSIKKIE WSREEDEKLL HLAKLMPTQW RTIAPIVGR TANQCLERYQ KLLDEAEQRE ASELGLTGPD GGETRAPTAE DVRKLRPGEI DPDPETKPAR PDTIDLDEDE K EMLSEARA ...String:
MPVVKGGVWT NIEDEILKAA VSKYGLNQWA RVSSLLARKT PKQCKARWNE WLDPSIKKIE WSREEDEKLL HLAKLMPTQW RTIAPIVGR TANQCLERYQ KLLDEAEQRE ASELGLTGPD GGETRAPTAE DVRKLRPGEI DPDPETKPAR PDTIDLDEDE K EMLSEARA RLANTQGKKA KRKARERQQE ESRRLAALQK RRELKTAGIN IKITTRKKGQ MDYNADIPFE KKPAPGFYDT TE EIARNEW QRAHFDPKKQ QVGNKRKSDE EEEAERKKRK NNKDGPSASL QAALKAGQMQ KLREAEQSSK RKPLILPAPQ VSD SELDEI VKMGMIGERA SAMARESEND ATRGLVGNYS TLNTGAPIRT PRAPAQEDHI ANEIRNIKAL TETQSSLLGG ENAP LAEGA GTTGFESVVP KKQVVSTPNP LATPLRQGPN GGATPLRPGQ TPLRTPRDTF SLNATDGVSM EHALRQQLKQ KLAAL PKPK ETEWELELPE EKQEPKTQEE LEEDAADRDR RERELREARE LAERRRRTQV MQRELPRTAV VDIDALLRAA DEIEDP ARA LVAREAALLM AHDAAKYPLP GAPPGVKPVE IPRFSDDELA EARLQILMEM KEKPAPEVVH AIWNRREENL NALRLGL GY YDSDSEDGED DVANIRATLE AALDRLMASA EKGNKLEKKL NLHLGGYKNR AEMLRKKLGE AHAALEKARN ALAGFQVL R ASEEQAIQRR LEALRAEVAF VSTRERKAQE LYRKLRDELE ELRLEQA

UniProtKB: Pre-mRNA splicing protein

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Macromolecule #12: Pre-mRNA-splicing factor SPF27

MacromoleculeName: Pre-mRNA-splicing factor SPF27 / type: protein_or_peptide / ID: 12 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719) (fungus)
Molecular weightTheoretical: 25.550703 KDa
SequenceString: MPAITTVHES LPYIDPEPTP EQRAAAEALI AEERAKVPDD PYHALLPPPL PPLNESRHLT PILQNELARL ASSPDPQAAK MDALDFSRY EAPEMPSIDS SQSLEETASQ LWETLKQAYT AQAYLSARRA HLALLDTHGK NAWLIGNWHL EGEVKAVEKE L AETKREID ...String:
MPAITTVHES LPYIDPEPTP EQRAAAEALI AEERAKVPDD PYHALLPPPL PPLNESRHLT PILQNELARL ASSPDPQAAK MDALDFSRY EAPEMPSIDS SQSLEETASQ LWETLKQAYT AQAYLSARRA HLALLDTHGK NAWLIGNWHL EGEVKAVEKE L AETKREID RVSLARQGMQ EAAGAELKSL EETWKAGVGR VLETEAAAEK LRIEVLEERR RLAEAQAALA VGN

UniProtKB: Pre-mRNA-splicing factor SPF27

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Macromolecule #13: Pre-mRNA-processing factor 19

MacromoleculeName: Pre-mRNA-processing factor 19 / type: protein_or_peptide / ID: 13 / Number of copies: 4 / Enantiomer: LEVO / EC number: RING-type E3 ubiquitin transferase
Source (natural)Organism: Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719) (fungus)
Molecular weightTheoretical: 51.844324 KDa
SequenceString: MLCALSGEIP EEPVVSKKTG VLFEKRLILK YLEEHNNIEP GTTEELDPET DLLPIKTSRV VRPRPPNFTS IPSLLKAFQD EWDALVLET YTTREQLARV REELATALYQ HDAAVRVIAR LTRERDEARE ALARLTVTGA APAAQNGEAM AVDSESLSEG L VEHVNEVQ ...String:
MLCALSGEIP EEPVVSKKTG VLFEKRLILK YLEEHNNIEP GTTEELDPET DLLPIKTSRV VRPRPPNFTS IPSLLKAFQD EWDALVLET YTTREQLARV REELATALYQ HDAAVRVIAR LTRERDEARE ALARLTVTGA APAAQNGEAM AVDSESLSEG L VEHVNEVQ QQLMKTRKKR PIPQGWATAD DVAALQQVAY TDLNVTQASS LDLENECAAV GGLDGKLDIY SVVANKVERT LD IGEPVTA TEWTGTKVVI GTAKGWVKVY DAGRESATFQ THAGPVTGLA VHPGGRILAS VGVDKSFVFY DLETGERVAR GYA DAALTT CAFHPDGNLF AAGTQTGHIL VFHTTTLEQA ESFPLGTPIQ ALAFSENGFW FAATGKGTSS VTIFDLRKSG AAAA VKELQ TGEVLSISWD YTGQYLATGG GTGVTVQMYT KATKSWSEPV RLGMPVVGVK WGGEAKRLVV VSREGVVSVL GKKEE

UniProtKB: Pre-mRNA-processing factor 19

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Macromolecule #14: Putative bud site selection protein

MacromoleculeName: Putative bud site selection protein / type: protein_or_peptide / ID: 14 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719) (fungus)
Molecular weightTheoretical: 17.176775 KDa
SequenceString:
MPAIRPASKR KPPPDGFSDI EEDLLIFANK MKDAQNTPTD NIPKHQAQWP IFQIAHQRSR YVYELYYQKQ AISKQLYDWL LKNGYADAM LIAKWKKQGY EKLCCLRCIQ TKETNFNSTC ICRVPRAQLK EDQDIQCVNC GCRGCASTD

UniProtKB: Bud site selection protein

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Macromolecule #15: Peptidyl-prolyl cis-trans isomerase

MacromoleculeName: Peptidyl-prolyl cis-trans isomerase / type: protein_or_peptide / ID: 15 / Number of copies: 1 / Enantiomer: LEVO / EC number: peptidylprolyl isomerase
Source (natural)Organism: Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719) (fungus)
Molecular weightTheoretical: 18.361811 KDa
SequenceString:
MATDVALETT MGTIILELYN QHAPRTCENF KTLAARGYYD GTIFHRVIKD FMVQGGDPTG TGRGGRSIYG DTFEDEIHPG LRHTGAGIL SMANAGPNTN GSQFFLTLAP TPWLDGKHTI FGRVKKGIRV LQRMGLVPTD ADDRPKTEIK IIRAYVVGEG E EDDKQLV

UniProtKB: Peptidyl-prolyl cis-trans isomerase

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Macromolecule #16: Pre-mRNA-splicing factor PRP46

MacromoleculeName: Pre-mRNA-splicing factor PRP46 / type: protein_or_peptide / ID: 16 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719) (fungus)
Molecular weightTheoretical: 54.207328 KDa
SequenceString: MATDVAVNGP DVSKLEALLR DNERTTRSLY ASTTPDSGRK RIKLDPGLAS EDPDITKSAL SLRLHAEYGD VQTLPEAIAK KLSAAGPRK KKPKPGVAED EAPSKSEEHT RKLIEGIPQA KPSAGAASSN ALVLAHGKPS AAGAGAARPQ RNEPQQLSLV R RSESLLAQ ...String:
MATDVAVNGP DVSKLEALLR DNERTTRSLY ASTTPDSGRK RIKLDPGLAS EDPDITKSAL SLRLHAEYGD VQTLPEAIAK KLSAAGPRK KKPKPGVAED EAPSKSEEHT RKLIEGIPQA KPSAGAASSN ALVLAHGKPS AAGAGAARPQ RNEPQQLSLV R RSESLLAQ PRPDWHPPWK LMRVISGHLG WVRALAVEPN NQWFASGAGD RTIKIWDLAS GQLRLTLTGH ISTVRGLAVS PR HPYMFSC GEDKMVKCWD LETNKVIRHY HGHLSGVYTL KLHPTLDVLV TGGRDGVARV WDMRTRSNVH VLSGHTGTVA DLV CQEADP QVITGSLDST VRMWDLAAGK TMGVLTHHKK GVRALVTHPT EFTFATASTG SIKQWKCPEG AFMQNFEGHN AIIN TLAVN DQNVLFSGGD NGSMSFWDWK SGYRFQSLDT TAQPGSLDAE LGIMSSTFDM SGARLITGEA DKTIKIWKED TTATP ETHP IEWKPSLVRR KY

UniProtKB: Pre-mRNA-splicing factor PRP46

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Macromolecule #17: Putative pre-mRNA splicing protein

MacromoleculeName: Putative pre-mRNA splicing protein / type: protein_or_peptide / ID: 17 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719) (fungus)
Molecular weightTheoretical: 42.241816 KDa
SequenceString: MPPQIKQDLN RAGWESTDFP SVCENCLPEN PYVKMLKEDY GAECKLCTRP FTVFSWAGDG RAHGRKKRTN ICLTCARLKN ACQCCIMDL QFGLPIVIRD KALELIAPGP QSEINREYFA QNNERAIEEG RAGVEEYEKT DEKARELLRR LANSKPYFRK G RELDSEGN ...String:
MPPQIKQDLN RAGWESTDFP SVCENCLPEN PYVKMLKEDY GAECKLCTRP FTVFSWAGDG RAHGRKKRTN ICLTCARLKN ACQCCIMDL QFGLPIVIRD KALELIAPGP QSEINREYFA QNNERAIEEG RAGVEEYEKT DEKARELLRR LANSKPYFRK G RELDSEGN PVAGGSGSTV GGNAVVGAGL GGAGPIRTRD TRAAAAAGAR TGGAGSARRG PGAALPPPGP KDWLPPADKS IM SLFITGI EDDLPEWKIR DFFKQYGKIK SLVVSHMTHC AFVNYETREG AEKAATELKG RAVIAGCPLR IRWSIPRPIG TMN KEERAE MLRDGRSAFP EANRKSAQKA IEAAGAQGGA AGGAAGGAAA EAQQDDLADL AIAAPPGAAD VQYASLAGN

UniProtKB: Pre-mRNA splicing protein

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Macromolecule #18: Putative pre-mRNA splicing protein

MacromoleculeName: Putative pre-mRNA splicing protein / type: protein_or_peptide / ID: 18 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719) (fungus)
Molecular weightTheoretical: 45.885184 KDa
SequenceString: MAELETNPDV AVAVAEDSNE QKQENQLVAV NNEVAAPIEK KVKKIIRKKR RPARPQIDPA LVKSERPPQT GTVFNIWYNK WSGGDREDK YLSQTHAKGR CNIARDSGYT RADSRPGSYF CLYFARGICP KGQDCDYLHR LPTIHDIFNP NVDCFGRDKF A DYRDDMGG ...String:
MAELETNPDV AVAVAEDSNE QKQENQLVAV NNEVAAPIEK KVKKIIRKKR RPARPQIDPA LVKSERPPQT GTVFNIWYNK WSGGDREDK YLSQTHAKGR CNIARDSGYT RADSRPGSYF CLYFARGICP KGQDCDYLHR LPTIHDIFNP NVDCFGRDKF A DYRDDMGG VGSFNRQNRT IYVGRIHVTD DIEEIVARHF AEWGQIERIR VLNNRGVAFI TYTNEANAQF AKEAMAHQSL DH NEILNVR WATADPNPLA QKREQRRIEE QAAEAIRRAL PAEFVAEIEG KDPEARKRRK LESSYGLEGY EAPDAVHFAR GPN AVNPRG REGFELEREQ QLMLEAPPET SQPAQIEAPP QAQEKKLAGG ILSSRTLAAL QGAKVAVATK DSKPEKPSGP LVAY GSDDE EDE

UniProtKB: Pre-mRNA-splicing factor CWC2

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Macromolecule #19: Pre-mRNA-processing protein 45

MacromoleculeName: Pre-mRNA-processing protein 45 / type: protein_or_peptide / ID: 19 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719) (fungus)
Molecular weightTheoretical: 65.034551 KDa
SequenceString: MASIAAGLAA ALPKPKYSSE HEEPRATQRG PRIVSADQID ETQIVIRRTG PPPYPNRAGW RPRAPEDFGD GGAFPEIPVA QYPWGKNDS SSKSNALVVQ VDSEGKVDYT AIARQGHSSD RIIHASFKDL IPLRQRAEAG QIDLSRPSKE EVEATAERTK N ALAALVSG ...String:
MASIAAGLAA ALPKPKYSSE HEEPRATQRG PRIVSADQID ETQIVIRRTG PPPYPNRAGW RPRAPEDFGD GGAFPEIPVA QYPWGKNDS SSKSNALVVQ VDSEGKVDYT AIARQGHSSD RIIHASFKDL IPLRQRAEAG QIDLSRPSKE EVEATAERTK N ALAALVSG ALAAQKPKNV QVNTKREATF VKYTPSAQMG NNTKKQERII KIVERQRDPM EPPKFKHKKI PRGPPSPPPP VM HSPPRKL TAEDQEAWRI PPPVSMWKNP KGFTIPLDKR LAADGRQLQE VQINDKFAQF SEALFMADRH AREEVRQRAM MQQ RLAEKE RQQKEEHLRQ LAQQARAERA AAAASGRRRS TRSRSGSYSG SESGSETDES ERERRRARRE KLKEEERKLR QSRM GAERR AQVLAREMDR DISEKIALGI AKPTMSGESM YDSRLFNQSS GFGATINEDN PYDKPLFAAQ EAISSIYRPR TNAEY EDDE EAGEKEMERI RKGNRFGEAL GRGTFKGTED AQPREGPVQF EKDTETADPF NVDKFLSEVQ KGSNSGGGKR GYGLQD EDS RQAKRSRVDD DENDD

UniProtKB: Pre-mRNA-processing protein 45

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Macromolecule #20: PRP17

MacromoleculeName: PRP17 / type: protein_or_peptide / ID: 20 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719) (fungus)
Molecular weightTheoretical: 61.222004 KDa
SequenceString: MPDFGEYPPN LPVRDALILR ESQAQAPTAN HAVVPYTGEN LARPSYGPAN PFRDTSSSSA SASGSLKRKN NVLTGHAEEM YISEATFRA KHRAVEAVGG GVMRSNREMK EVNKRLREMR EDKGSATIAD GEGAYIGPWA RYKRREEYEV VDKKGEEEDG D EYEEVEVT ...String:
MPDFGEYPPN LPVRDALILR ESQAQAPTAN HAVVPYTGEN LARPSYGPAN PFRDTSSSSA SASGSLKRKN NVLTGHAEEM YISEATFRA KHRAVEAVGG GVMRSNREMK EVNKRLREMR EDKGSATIAD GEGAYIGPWA RYKRREEYEV VDKKGEEEDG D EYEEVEVT DEEEEVIESG TVLKAPEPAI ARRKEAEEMG EERTEFLGES EYDYLGRTYM HVPQDLDVSL NKEVGSITNY IP KKLIYTW RHHGGKPITA LQLFPRSSHL GLAGSADGVV KIFDVYRGRE LLRSYSGHNK AITDLSFCND GTKFLSGGFD RKI RLWDTE TGQCVNRFNI GKTPHVIKFN PSSENGHEFL AGLSDKRIVQ YDTRAGNDTV QEYDRHLGPI NTIEYIDENR RFMS TSDDR SLKVWEYGIP VEIKTISEPD MFALTKSAQH PNGKYVLYQC SDNSIVAYSC SGDKFRQHRK KAWRGHNTAG SAIGL TCSP DGQFVASGDT SGSVCFWDFK TCKLYSKLTA DSAGGIINCV AWSEQETSKV FTAGAKGEIK LWD

UniProtKB: Uncharacterized protein

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Macromolecule #21: Putative pre-mRNA splicing protein

MacromoleculeName: Putative pre-mRNA splicing protein / type: protein_or_peptide / ID: 21 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719) (fungus)
Molecular weightTheoretical: 28.956562 KDa
SequenceString: MTTAHRPTFD PARGKEALRG PAYHQRLLPA YTTLKFRQPG QGGAADKSTR DLRAELEAAE AAHYAKLKGA PIPGTSSSSE NNTPAIAGS SASSVTGKRP LPSSSNGGGD RPPDDGDEDP EAKRRRILAE TRDIDADDDS SSGSDKDSDS ESGSADSDDS S DDEEAELQ ...String:
MTTAHRPTFD PARGKEALRG PAYHQRLLPA YTTLKFRQPG QGGAADKSTR DLRAELEAAE AAHYAKLKGA PIPGTSSSSE NNTPAIAGS SASSVTGKRP LPSSSNGGGD RPPDDGDEDP EAKRRRILAE TRDIDADDDS SSGSDKDSDS ESGSADSDDS S DDEEAELQ RELERVRRER MEKRAREEAE RKAAEEARRE EEIARGNPLL NKPDFTVKRR WDEDVVFKNQ ARGTEDKGKR KE FVNDLLR SDFHKRFMSK YVR

UniProtKB: Pre-mRNA splicing protein

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Macromolecule #22: Pre-mRNA-splicing factor

MacromoleculeName: Pre-mRNA-splicing factor / type: protein_or_peptide / ID: 22 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719) (fungus)
Molecular weightTheoretical: 161.045484 KDa
SequenceString: MPPAKRIKSS ADSRPNAGAS GRPTLEDLEG ENQFTNLARQ HWLNVPQQAA KIKVKTDVLK RELYLWPGYG EDSSNYHVLL IILIVNAKR RERVSTWDIF ADRPADFSDL FRRALSMTLD SSLSWTIRTH VLLFIIHAFQ SLDYAIVRKE CAPLVSISIW H NLSTEEKR ...String:
MPPAKRIKSS ADSRPNAGAS GRPTLEDLEG ENQFTNLARQ HWLNVPQQAA KIKVKTDVLK RELYLWPGYG EDSSNYHVLL IILIVNAKR RERVSTWDIF ADRPADFSDL FRRALSMTLD SSLSWTIRTH VLLFIIHAFQ SLDYAIVRKE CAPLVSISIW H NLSTEEKR EALLDSNPHL RKAWRAATKR FESADDATKA RLRFDRAWLY SLVLDFLTLL YSGNAKQEHV LYCERFVEFL TD LQSQLPT RRYVNTLLQD LHVLPALSLS PIYNDEGNGL LRELCNLFTH YTYFAVDDQS GVQLSREQAY DRHCAILAKL QRI AMKHFK EKLTVLALSN YGSIDKRSEL EPLLQALTDD ELVQLSNLMN IRTSYPDAAR IPVDRKFIVE VLLTTFERRK TFQD AAQAL SVLPTEETLF DISLKRTDQY DGSRPLALPK LNLQYLSVGD FLWRSFVLYR CESFYAIRQD LEDALIRLKP EVRRG GVTG FAGFSKMALP ISKPVILDVM PPQVGDDKPS CVKAEVTIDL RRLTPQIRRD WESLRPDDVV FLLAVDASRQ KQSANG GAV LSEAERLGLV HVRAAEIIQV LDDKGKAIRD PQAYFDGHTR SDIRKIQLRL DATSYKADTE ANRNVYEDIN LIVRRSS RE NNFKPVLESI QDLTLSEVPL ASWLHEVFLG YGDPAGATFK QLPNRLKKIN FRDTFLDWQH LVESFPGKII EPSDDVSS S FGPPYVLESV EKQVEEHPSK PSKKRRRDVE PALMSKVETL KVSTYKPPNN GPYPVDAPKL NKIRFTPTQI DAIYSGTQP GLTIIVGPPG TGKTDVAVQI ISNIYHNFPE QKTLLVAHSN QALNQLFAKI VALDIDERHL LRLGHGEEEL ETEGSFSKHG RVESFLDNR QRFLYEVSRL AASMGAPGAH GNSAETAGYF NKVYVEPAWA KFNDIIQRED VGPEDIVRAF PFHAYFSDAP Q PLFPPEAD RETVLEIANG CYRHISKIFE ELADVLPFEI LRRDKDKANY LLTSEARIIA MTSTHAAMKR GEIASLGFQY DN VIMEEAA QITEIENFIP LALQKPKNGQ MALQRVVLCG DHYQNSPVIQ GLAFRHYANL EQSLFSRLVR LGVPTINLDQ QGR ARPSIS NLYRWRYPQL GDLPHTQTEP EFLTANAGFR YDYQFVNVPD YRGMGESEPT PHFIQNLGEA EYAVAIFQYM RLLG YPASK ISILATYAGQ KALIKDVLAH RCAKNPIFGL PRVVTTVDKY QGEQNDYIIL SLTRTTRVGY LRDLRRLTVA LSRAR LGLY ILGRRAVFES CYELRDAFSL LLRRPDKLAL VTGELWPSKR LLADETDDTK KLEGEVVMEG VEHLGQWVFE MTKTKI AEL RKEKGLSEEV PVEMPKEVVS AEADEGYLPL DEEEGDGEEG EGERVKGFEI EDS

UniProtKB: Pre-mRNA-splicing factor

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Macromolecule #23: GPATCH1

MacromoleculeName: GPATCH1 / type: protein_or_peptide / ID: 23 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719) (fungus)
Molecular weightTheoretical: 75.861016 KDa
SequenceString: MSHKRSRAAY EADLTAQQSP YVFFGTPLPP LDPDVRDDGS YVPIWKQEAR DERGRKRFHG AFTGGWSAGY FNTVGSKEGW TPSSFVSSR TKRWKDDPNK VEQRPEDFMD EEDLADLEES RKLQTREAFS GLGSTADDAV RASGLMGLFR VEGETMGVKL L KKMGWKEG ...String:
MSHKRSRAAY EADLTAQQSP YVFFGTPLPP LDPDVRDDGS YVPIWKQEAR DERGRKRFHG AFTGGWSAGY FNTVGSKEGW TPSSFVSSR TKRWKDDPNK VEQRPEDFMD EEDLADLEES RKLQTREAFS GLGSTADDAV RASGLMGLFR VEGETMGVKL L KKMGWKEG QGIGPKVRRK ARLGLGSDAN ITEETHLFAP DNVPMISFVR KTDHKGLGYA GETGLTPLSK ANGQNESDEE DE DDGGIGS LGRPRFSLTT ERKKGPQRPR GSIGVGILND TGSDDEDPYE LGPKISYNRV IGGDKQKKKT ATATNPTLKS KPT FVSSKS KALGKVALGV RKCHDGRLPL DGFVFGKEPD PLISEIIAEG KYPPPRIPPG WVSSKKPTSS AKQPAGYVST AEAA KSSTL DPRARAAILG EKQLPGKSVF DFLSPEARER LVAATGKKDL PPARGEVPAE YALSEDERLK KLLAQVPRLD KETAV AAIT RGASGAAPYA DDEAKRSRYL SFLEWQAGFK SSPGARTPKM KDDDWLRELH EFYNCARIFK PMTGFMASRF TTSSAA KPG SSSDGGASNN SCSQTPTKPQ DPTEEAAKLG MFGPMTRTVT EFYPSRLLCK RFGVNPPEHV RSDEYPANKS RFDASSI GG CSAASTGPTE EGTKPAWMPT GKAVVVVDPS RNEALEAKRA GEEVFKAVFG DSGDEG

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Macromolecule #24: RNA helicase

MacromoleculeName: RNA helicase / type: protein_or_peptide / ID: 24 / Number of copies: 1 / Enantiomer: LEVO / EC number: RNA helicase
Source (natural)Organism: Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719) (fungus)
Molecular weightTheoretical: 74.98975 KDa
SequenceString: MADSEVGGAF IPALYKPAAL LPIAKHRESL LYLVETNPVT IVVGQTGSGK STQIPQFLEK AGWCADGKLI AVTQPRRVAA VTLAIRVAE EFGCEVGKEV GYSIRFEDAT SESTRIKYMT DGLLIREALV DPLLSRYSVI MIDEAHERSI SSDILLGLLK K IRRKRPDL ...String:
MADSEVGGAF IPALYKPAAL LPIAKHRESL LYLVETNPVT IVVGQTGSGK STQIPQFLEK AGWCADGKLI AVTQPRRVAA VTLAIRVAE EFGCEVGKEV GYSIRFEDAT SESTRIKYMT DGLLIREALV DPLLSRYSVI MIDEAHERSI SSDILLGLLK K IRRKRPDL RIIISSATLQ AEDYRAYFEK ASETQEEDSS NDKQKESIAS IISIEGRTYP IDILYLDTPT EDYLEKAIST VF DIHTNEP KGDILVFLTG RDEIEQAVQA VSERSASLPP GSEALLPLPL YSGLSAEQQM YVFEEAPENT RKVIFSTNLA EAS VTIEGI VYVIDSGFVK LRAYNPKTGI ESLTATPVSK ASAAQRAGRA GRTKPGKCFR LYTEEAYQSL PDATVPEIQR SNLA PIILQ LKALGIDNVL RFDYFTPPPA EQMTRALELL YSLGALDDYA KLTRPLGLRM AELAVEPMMA KTLLSAQSFG CLSEI LTIA AMTSLDGTLW IQHEGDKKKT ESVKRKFAAE EGDHLTLLNV YQAFVTKGRK EAHWCHENML NYKAMIRAVS IRAQLK RFL ERFGIDVEES LSSPSVSQQP ANKAEKIQRC LTIGYFAHAA KMQPDGTFRN VSGTTVLHAH PSSIMFNRKA DWVIFHE VL ETKDKTFIRD ITRIKKEWLL EYAPDFYKTT

UniProtKB: RNA helicase

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Macromolecule #25: Small nuclear ribonucleoprotein E

MacromoleculeName: Small nuclear ribonucleoprotein E / type: protein_or_peptide / ID: 25 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719) (fungus)
Molecular weightTheoretical: 10.967812 KDa
SequenceString:
MTSRGAPAGR RVLLPPINFL FRLLQQRTPV QIWLYEQLAI RITGVIRGFD EFMNLVIDDA VEIKLSPKTN EPESKRPLGQ ILLKGDNIS LIQALSGSA

UniProtKB: Small nuclear ribonucleoprotein E

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Macromolecule #26: Small nuclear ribonucleoprotein G

MacromoleculeName: Small nuclear ribonucleoprotein G / type: protein_or_peptide / ID: 26 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719) (fungus)
Molecular weightTheoretical: 9.259763 KDa
SequenceString:
MAPAQPELKK YLDKRLFVEL NGSRRVIGVL RGYDVFLNIV LDDAVEERPN GEKVKLGMVT IRGNSVVILE PLERIGDDRP GR

UniProtKB: Small nuclear ribonucleoprotein G

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Macromolecule #27: Sm protein F

MacromoleculeName: Sm protein F / type: protein_or_peptide / ID: 27 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719) (fungus)
Molecular weightTheoretical: 10.727227 KDa
SequenceString:
MNTGFVPVNP RPFLQDLVNQ DVIVRLKWGQ TEYKGRLVSI DSYMNIQLAN TEEFINYKST GHLGQVLIRC NNVLWVTAAA GKQMTPTED TKMEE

UniProtKB: Sm protein F

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Macromolecule #28: Delta(14)-sterol reductase

MacromoleculeName: Delta(14)-sterol reductase / type: protein_or_peptide / ID: 28 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719) (fungus)
Molecular weightTheoretical: 66.785492 KDa
SequenceString: MAPKTKKQAV SRTQHYEFFG PPGAAAISFG LPLLVYAFAF ACNDITGCPA PSLLHPKSLK LDVLKQEVGW PKNGIWGLAS WRVTGWTLA YYLWLALLYR ILPGDVVEGT QLRNGGRLKY KLNAFASSMF TLVICAAGTI AQGADFPLWT FIADNYVQIL T ANVIIAYT ...String:
MAPKTKKQAV SRTQHYEFFG PPGAAAISFG LPLLVYAFAF ACNDITGCPA PSLLHPKSLK LDVLKQEVGW PKNGIWGLAS WRVTGWTLA YYLWLALLYR ILPGDVVEGT QLRNGGRLKY KLNAFASSMF TLVICAAGTI AQGADFPLWT FIADNYVQIL T ANVIIAYT LATFVYIRSF SVKPGNPQLR ELAVGGVTGN ILYDWFIGRE LNPRVTLPII GEIDIKQWME LRPGLLGWTL MN CAFVAKQ YRLYGYVTDS IVFVTAVQAL YVLDSHYFEP AVLTTIDITT DGFGLMLSFG DIVWVPFIYT QSTRYLAVHP QKL GYLGLV GVTVLLVAGF AIFRLSNSQK NAFRTNPNDP SVKHLKYIET KAGTRLLISG WWGIARHINY LGDWLQAWPY CLPT GLAGY QILTAGSELA EGAVKMLDGR IVVPGEARGW GTIFTYFYVV YFATLLLHRD RRDDEKCSKK YGEDWEKYKE IVRWR IIPA LLQKPRSECT EWEIAQIEEW EMANGPLSLL QTAVRSHTQV LISVRSGRKL LARVKAFDRH CNMILENVKE MWTETP VTN GKKGRPVNKD RFISKMFLRG DSVVIVLLS

UniProtKB: Delta(14)-sterol reductase

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Macromolecule #29: Small nuclear ribonucleoprotein Sm D1

MacromoleculeName: Small nuclear ribonucleoprotein Sm D1 / type: protein_or_peptide / ID: 29 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719) (fungus)
Molecular weightTheoretical: 13.033093 KDa
SequenceString:
MKLVRFLMKC ANETVTIELK NGAIVHGTIA SVTPKMDTAL RNVRYTPKGE EPYSVESLTV RGNTIRYYIL PDSLPLDTLL IDDAPKPKN KARKEADRGR GRGRGRGGRG GRGGRARGR

UniProtKB: Small nuclear ribonucleoprotein Sm D1

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Macromolecule #30: Sm protein B

MacromoleculeName: Sm protein B / type: protein_or_peptide / ID: 30 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719) (fungus)
Molecular weightTheoretical: 21.425543 KDa
SequenceString: MSSSQNRQGK MAGYINWRMR VTLNDGRQMT GQMLAFDKHM NLVLADTEEF RKTKRMQAKP NSSTTQTLIQ EEKRTLGLTI VRGANIIAL SVESPPPADP SARLGKTTGA GIASTLTAGP GVARPAGRGA AAPISLAGPA PGVGGAVPPP PFPGFPAAAP P GFPGRGGP ...String:
MSSSQNRQGK MAGYINWRMR VTLNDGRQMT GQMLAFDKHM NLVLADTEEF RKTKRMQAKP NSSTTQTLIQ EEKRTLGLTI VRGANIIAL SVESPPPADP SARLGKTTGA GIASTLTAGP GVARPAGRGA AAPISLAGPA PGVGGAVPPP PFPGFPAAAP P GFPGRGGP APPGFPAAPF PPPAGFPGAP GFPPGFPPGG APPPAGFNPP PRR

UniProtKB: Sm protein B

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Macromolecule #31: Small nuclear ribonucleoprotein Sm D3

MacromoleculeName: Small nuclear ribonucleoprotein Sm D3 / type: protein_or_peptide / ID: 31 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719) (fungus)
Molecular weightTheoretical: 12.70868 KDa
SequenceString:
MTSSIGIPIK LLNEAQGHIV TLELTTGQTY RGKLIEAEDN MNVQLKDITV TARDGRVSHL EQIYIRGSHV RFFIVPDMLR NAPMFRSRN VRGRGVGLAR GRATVSRARA SGRGR

UniProtKB: Small nuclear ribonucleoprotein Sm D3

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Macromolecule #32: Putative pre-mRNA splicing protein

MacromoleculeName: Putative pre-mRNA splicing protein / type: protein_or_peptide / ID: 32 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719) (fungus)
Molecular weightTheoretical: 26.329277 KDa
SequenceString: MSDNVGLPTP RGSGTSGYVQ RNLAHLRPRE PIKPRDPESY RHKPRKPDPG LLEHDRKREV EVKVFELRDK LEEEGVDEEE IESRCSELR QKLLAEMERN KDALPAQRKT FKAHQVHEAA DAKLKESERL RQALKISKDY EEGSHWRRQE ERKKPLERGD L PPGVADRE ...String:
MSDNVGLPTP RGSGTSGYVQ RNLAHLRPRE PIKPRDPESY RHKPRKPDPG LLEHDRKREV EVKVFELRDK LEEEGVDEEE IESRCSELR QKLLAEMERN KDALPAQRKT FKAHQVHEAA DAKLKESERL RQALKISKDY EEGSHWRRQE ERKKPLERGD L PPGVADRE KERERGRERG RDGDRNGDRY RQRDDDRYQP EPEPEQKQEQ KQKLEQKSSA WAGSV

UniProtKB: Pre-mRNA splicing protein

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Macromolecule #33: Putative pre-mRNA splicing protein

MacromoleculeName: Putative pre-mRNA splicing protein / type: protein_or_peptide / ID: 33 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719) (fungus)
Molecular weightTheoretical: 76.518102 KDa
SequenceString: MASADSAAAA AKTEEQKLAE QRAEYQKLLE LRSQGVYLPP HKLRALQAAI TDPKTKEYQR IAWDALKKSI NGLVNKVNTA NIKHVVPEL FNENLIRGRG LFCQSLLKAQ HASLPFTPIY ACMAAIVNTK LPQVGELLVK RLIMRFRKAF KRNDKPVCLS S TMFIAHLV ...String:
MASADSAAAA AKTEEQKLAE QRAEYQKLLE LRSQGVYLPP HKLRALQAAI TDPKTKEYQR IAWDALKKSI NGLVNKVNTA NIKHVVPEL FNENLIRGRG LFCQSLLKAQ HASLPFTPIY ACMAAIVNTK LPQVGELLVK RLIMRFRKAF KRNDKPVCLS S TMFIAHLV NQQVVHETLA GQILVLLLQK PTDDSVEIAV GFMREVGLFL EEMAPRIAHI VFDQFKNILH EADIDRRTQY MI EVLFQIR KDRYKDNPVI KEELDLIEEE DQITHRISLD EDINTEDSLN IFKYDPEWEE NENEYKKLKA EILGEVSGDE DEE DEDEED EESEESEDEE QKAIEIRDQT NADLVNLRRT IYLTIQSSAD PEEAAHKLMK LKLPPGQEPE LVSMIIESCA QEKV YSKFM GLLGEKFARL NRMWMELYEE AFTKYYNTIH RYETNKLRNI ARFFGHLLSY DAIGWHVLSV IQLTEEETTA ASRIF IRFL FEDIQENLGT AKLKARLGDE ALQPYLEGIF RHDTRRNVTF AINYFTAIKM GYLTDEMHPG RHHGRGLGPE VALRRV PLF VGPDPTAGAG AGAGTGAAGA TPAAPAGAEA APEIIPALFP QEVGADPSLA RFLAHHPLVV KQESLTGAVS GSKEAEK VE VEVEVEVKVE DTAEARRAVQ QVSVAVKVPI EVCVPP

UniProtKB: Pre-mRNA-splicing factor CWC22

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Macromolecule #34: Nineteen complex-related protein 2-domain-containing protein

MacromoleculeName: Nineteen complex-related protein 2-domain-containing protein
type: protein_or_peptide / ID: 34 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719) (fungus)
Molecular weightTheoretical: 56.472395 KDa
SequenceString: MAAFGAKRKP RIIKAFDDDD EDLSLPLSSG GEDKQAGEEL PPPARIKFGR TKFTKSSALR KNAMIGNDDT DSPNATSARD DDDDDENSG APVVVRPSSV NKGSLSKIKK RPAASRLSFG PSAGAEDDDE EAEVVIQPRK MLNQRAVENS ALRANSSLPT R FGGEENRP ...String:
MAAFGAKRKP RIIKAFDDDD EDLSLPLSSG GEDKQAGEEL PPPARIKFGR TKFTKSSALR KNAMIGNDDT DSPNATSARD DDDDDENSG APVVVRPSSV NKGSLSKIKK RPAASRLSFG PSAGAEDDDE EAEVVIQPRK MLNQRAVENS ALRANSSLPT R FGGEENRP KYSKEYLAEL QSATFNTPQN LADLKIHDDD EMQLDEMELE GALIVPSNEV AVPGASTTQT THIPTEAEIR ER KERRARL AHEAKFIPLD DEFNSDNEGA QPSHPILNLP SKQKRRDTRL IREDEDLYEG FDEFVSDGNL ALGRKAEKAV LQR HRQEMA ELIEAAQAED NDEAASDDSE AEERAAYEEA QVRAAMDGLR GKYREEHLER GGGADLYEGR GPDDIPRMKP LPKL GDVLQ RIREAIQGLE GEVVRKRSRI EGLEKEKAEI LVREKEVQEI LNQAGQKYQE VVGGLGVHNV PKIVAGQSPL RPFPP GLAR EMPTERGLES YGATPIRRYG GEEDDG

UniProtKB: Nineteen complex-related protein 2-domain-containing protein

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Macromolecule #35: GCFC2

MacromoleculeName: GCFC2 / type: protein_or_peptide / ID: 35 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719) (fungus)
Molecular weightTheoretical: 5.634938 KDa
SequenceString: (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) ...String:
(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)

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Macromolecule #36: TFIP11

MacromoleculeName: TFIP11 / type: protein_or_peptide / ID: 36 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719) (fungus)
Molecular weightTheoretical: 59.591422 KDa
SequenceString: (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) ...String:
(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)

+
Macromolecule #38: Putative pre-mRNA splicing protein

MacromoleculeName: Putative pre-mRNA splicing protein / type: protein_or_peptide / ID: 38 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719) (fungus)
Molecular weightTheoretical: 28.376967 KDa
SequenceString: MARNSEKAQS MLFRFREAQA ADLGIIDAGR ARRPRVITEV DSIPACEKWR GQVLKEISRK VSRIQDPALS DYQIRDLNDE INKLMREKH MWEVQIRNLG GPNYMRGGGK VYDEAGREIP GAGRGYRYFG RARELPGVKE LFEAAAKAKR EEEEKPLEER A DLRRQVNA ...String:
MARNSEKAQS MLFRFREAQA ADLGIIDAGR ARRPRVITEV DSIPACEKWR GQVLKEISRK VSRIQDPALS DYQIRDLNDE INKLMREKH MWEVQIRNLG GPNYMRGGGK VYDEAGREIP GAGRGYRYFG RARELPGVKE LFEAAAKAKR EEEEKPLEER A DLRRQVNA AYYGYAPGEE DDELLEYERA REEQARKALQ KAGPPDAPPG WEPLPGDTGD GRVWELPTLE EVQRELIERR RQ RLLDQL

UniProtKB: Pre-mRNA splicing protein

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Macromolecule #39: N,N,7-trimethylguanosine 5'-(trihydrogen diphosphate)

MacromoleculeName: N,N,7-trimethylguanosine 5'-(trihydrogen diphosphate) / type: ligand / ID: 39 / Number of copies: 1 / Formula: M7M
Molecular weightTheoretical: 487.296 Da
Chemical component information

ChemComp-M7M:
N,N,7-trimethylguanosine 5'-(trihydrogen diphosphate)

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Macromolecule #40: MAGNESIUM ION

MacromoleculeName: MAGNESIUM ION / type: ligand / ID: 40 / Number of copies: 1 / Formula: MG
Molecular weightTheoretical: 24.305 Da

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Macromolecule #41: GUANOSINE-5'-TRIPHOSPHATE

MacromoleculeName: GUANOSINE-5'-TRIPHOSPHATE / type: ligand / ID: 41 / Number of copies: 1 / Formula: GTP
Molecular weightTheoretical: 523.18 Da
Chemical component information

ChemComp-GTP:
GUANOSINE-5'-TRIPHOSPHATE / GTP, energy-carrying molecule*YM

+
Macromolecule #42: ZINC ION

MacromoleculeName: ZINC ION / type: ligand / ID: 42 / Number of copies: 4 / Formula: ZN
Molecular weightTheoretical: 65.409 Da

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

BufferpH: 7.4
VitrificationCryogen name: ETHANE

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Electron microscopy

MicroscopeTFS KRIOS
Image recordingFilm or detector model: FEI FALCON IV (4k x 4k) / Average electron dose: 50.0 e/Å2
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 2.5 µm / Nominal defocus min: 1.0 µm
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

Startup modelType of model: PDB ENTRY
PDB model - PDB ID:

5y88

Final reconstructionResolution.type: BY AUTHOR / Resolution: 2.9 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 66478
Initial angle assignmentType: OTHER / Software - Name: RELION / Details: Relion
Final angle assignmentType: OTHER / Software - Name: RELION / Details: Relion
FSC plot (resolution estimation)

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