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- EMDB-58448: cryoEM structure of influenza B RNP-like particle with NPdel68 -

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Basic information

Entry
Database: EMDB / ID: EMD-58448
TitlecryoEM structure of influenza B RNP-like particle with NPdel68
Map data
Sample
  • Complex: RiboNucleoProtein (RNP)-like helical assembly
    • Protein or peptide: Nucleoprotein
    • RNA: synthetic RNA
KeywordsRNA-protein complex / helical assembly / influenza B virus / VIRAL PROTEIN
Function / homology:
Function and homology information
Biological speciesInfluenza B virus (B/Memphis/13/2003) / synthetic RNA (others)
Methodsingle particle reconstruction / cryo EM / Resolution: 4.1 Å
AuthorsThirion M / Stelfox A / Chenavier F / Ruigrok R / Crepin T / Ballandras-Colas A
Funding support France, 1 items
OrganizationGrant numberCountry
Agence Nationale de la Recherche (ANR)ANR-23-CE11-0010-01 France
CitationJournal: To Be Published
Title: Nucleocapsid-like cryoEM structure of influenza B virus
Authors: Thirion M / Stelfox A / Chenavier F / Chomat H / Isel C / Freslon LL / Zarkadas E / Ruigrok R / Naffakh N / Crepin T / Ballandras-Colas A
History
DepositionJun 9, 2026-
Header (metadata) releaseAug 5, 2026-
Map releaseAug 5, 2026-
UpdateAug 5, 2026-
Current statusAug 5, 2026Processing site: PDBe / Status: Released

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Structure visualization

Supplemental images

emd_58448.png

Downloads & links

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Map

FileDownload / File: emd_58448.map.gz / Format: CCP4 / Size: 61 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Projections & slices

Image control

Size
Brightness
Contrast
Others
AxesZ (Sec.)Y (Row.)X (Col.)
1.86 Å/pix.
x 252 pix.
= 468.72 Å		1.86 Å/pix.
x 252 pix.
= 468.72 Å		1.86 Å/pix.
x 252 pix.
= 468.72 Å

Surface

Projections

Slices (1/3)

Slices (1/2)

Slices (2/3)

Images are generated by Spider.

Voxel sizeX=Y=Z: 1.86 Å
Density

Histogram

Histogram (log scale)
Contour LevelBy AUTHOR: 0.25
Minimum - Maximum-0.96639127 - 1.6735673
Average (Standard dev.)0.0005297116 (±0.027471265)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions252252252
Spacing252252252
CellA=B=C: 468.72 Å
α=β=γ: 90.0 °

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Supplemental data

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Mask #1

Fileemd_58448_msk_1.map
Projections & Slices
AxesZYX

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Additional map: #1

Fileemd_58448_additional_1.map
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Additional map: #2

Fileemd_58448_additional_2.map
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Half map: #1

Fileemd_58448_half_map_1.map
Projections & Slices
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Half map: #2

Fileemd_58448_half_map_2.map
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Sample components

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Entire : RiboNucleoProtein (RNP)-like helical assembly

EntireName: RiboNucleoProtein (RNP)-like helical assembly
Components
  • Complex: RiboNucleoProtein (RNP)-like helical assembly
    • Protein or peptide: Nucleoprotein
    • RNA: synthetic RNA

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Supramolecule #1: RiboNucleoProtein (RNP)-like helical assembly

SupramoleculeName: RiboNucleoProtein (RNP)-like helical assembly / type: complex / ID: 1 / Parent: 0 / Macromolecule list: all
Source (natural)Organism: Influenza B virus (B/Memphis/13/2003)

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Macromolecule #1: Nucleoprotein

MacromoleculeName: Nucleoprotein / type: protein_or_peptide / ID: 1
Details: Protein deleted of the first 68 residues and fused to His-6 tag in C-ter.
Number of copies: 6 / Enantiomer: LEVO
Source (natural)Organism: Influenza B virus (B/Memphis/13/2003)
Molecular weightTheoretical: 62.938391 KDa
Recombinant expressionOrganism: Escherichia coli BL21(DE3) (bacteria)
SequenceString: MSNMDIDGIN TGTIDKAPEE ITSGTSGTTR PIIRPATLAP PSNKRTRNPS PERATTISEA DVGRKTQKKQ TPTEIKKSVY NMVVKLGEF YNQMMVKAGL NDDMERNLIQ NAHAVERILL AATDDKKTEF QKKKNARDVK EGKEEIDHNK TGGTFYKMVR D DKTIYFSP ...String:
MSNMDIDGIN TGTIDKAPEE ITSGTSGTTR PIIRPATLAP PSNKRTRNPS PERATTISEA DVGRKTQKKQ TPTEIKKSVY NMVVKLGEF YNQMMVKAGL NDDMERNLIQ NAHAVERILL AATDDKKTEF QKKKNARDVK EGKEEIDHNK TGGTFYKMVR D DKTIYFSP IRVTFLKEEV KTMYKTTMGS DGFSGLNHIM IGHSQMNDVC FQRSKALKRV GLDPSLISTF AGSTLPRRSG AT GVAIKGG GTLVAEAIRF IGRAMADRGL LRDIKAKTAY EKILLNLKNK CSAPQQKALV DQVIGSRNPG IADIEDLTLL ARS MVVVRP SVASKVVLPI SIYAKIPQLG FNVEEYSMVG YEAMALYNMA TPVSILRVGD DAKDKSQLFF MSCFGAAYED LRVL SALTG TEFKPRSALK CKGFHVPAKE QVEGMGAALM SIKLQFWAPM TRSGGNEVGG DGGSGQISCS PVFAVERPIA LSKQA VRRM LSMNIEGRDA DVKGNLLKMM NDSMAKKTNG NAFIGKKMFQ ISDKNKTNPV EIPIKQTIPN FFFGRDTAED YDDLDY INL EHHHHHH

UniProtKB: UNIPROTKB: Q5V913

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Macromolecule #2: synthetic RNA

MacromoleculeName: synthetic RNA / type: rna / ID: 2 / Number of copies: 6
Source (natural)Organism: synthetic RNA (others)
Molecular weightTheoretical: 7.291213 KDa
SequenceString:
UCUCUCUCUC UCUCUCUCUC UCUC

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

Concentration1 mg/mL
BufferpH: 7.5
Component:
ConcentrationFormulaName
20.0 mMHEPESHEPES
150.0 mMNaClsodium chloride
5.0 mMHOCH2CH2SH2-Mercaptoethanol

Details: 20 mM HEPES pH 7.5, 150 mM NaCl, 5 mM 2-Mercaptoethanol
GridModel: UltrAuFoil R1.2/1.3 / Material: GOLD / Mesh: 300 / Support film - Material: GOLD / Support film - topology: HOLEY / Pretreatment - Type: GLOW DISCHARGE / Pretreatment - Time: 45 sec.
VitrificationCryogen name: ETHANE / Chamber humidity: 100 % / Chamber temperature: 293.15 K / Instrument: FEI VITROBOT MARK IV

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Electron microscopy

MicroscopeTFS GLACIOS
SoftwareName: EPU
Image recordingFilm or detector model: FEI FALCON IV (4k x 4k) / Number grids imaged: 1 / Average electron dose: 38.5 e/Å2
Electron beamAcceleration voltage: 200 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Cs: 2.7 mm / Nominal defocus max: 2.0 µm / Nominal defocus min: 0.6 µm / Nominal magnification: 36000
Sample stageCooling holder cryogen: NITROGEN

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Image processing

CTF correctionSoftware - Name: cryoSPARC (ver. 4.7) / Type: PHASE FLIPPING AND AMPLITUDE CORRECTION
Startup modelType of model: NONE
Final reconstructionResolution.type: BY AUTHOR / Resolution: 4.1 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: cryoSPARC (ver. 4.7) / Number images used: 357020
Initial angle assignmentType: RANDOM ASSIGNMENT
Details: Maximum likelyhood plus stochastic gradient descent (Punjani et al, Nat. Methods, 2017 https://doi.org/10.1038/nmeth.4169)
Final angle assignmentType: MAXIMUM LIKELIHOOD
Details: Maximum likelyhood plus stochastic gradient descent (Punjani et al, Nat. Methods, 2017 https://doi.org/10.1038/nmeth.4169)
FSC plot (resolution estimation)

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Atomic model buiding 1

RefinementSpace: REAL / Protocol: RIGID BODY FIT
Output model

PDB-31jo:
cryoEM structure of influenza B RNP-like particle with NPdel68

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