EMDB-56766: Cryo-EM single particle structure of the Plastid-Encoded RNA Poly...

Basic information

Entry

Database: EMDB / ID: EMD-56766

• Title: Cryo-EM single particle structure of the Plastid-Encoded RNA Polymerase (PEP)from Chlamydomonas reinhardtii.
• Map data: Cryo-EM single particle structure of the Plastid-Encoded RNA Polymerase (PEP)from Chlamydomonas reinhardtii.

Sample

• Complex: Plastid-Encoded RNA Polymerase (PEP) from Chlamydomonas reinhardtii
  • Protein or peptide: x 17 types

• Keywords: Chlamydomonas reinhardtii / Plastid-Encoded RNA / Polymerase / Cryo-EM / RNA

Function / homology

Function:

lipid X metabolic process / plastid-encoded plastid RNA polymerase complex / carboxylic acid biosynthetic process / acid-amino acid ligase activity / glucose-6-phosphate 1-epimerase activity / UDP-3-O-acyl-N-acetylglucosamine deacetylase / UDP-3-O-acyl-N-acetylglucosamine deacetylase activity / ligase activity / lipid A biosynthetic process / catalytic activity / RNA processing / mitochondrion organization / chloroplast / ribonucleoside binding / DNA-directed RNA polymerase / DNA-directed RNA polymerase activity / carbohydrate binding / carbohydrate metabolic process / DNA-templated transcription / metal ion binding / DNA binding / RNA binding / zinc ion binding / ATP binding

Domain/homology:

: / : / Protein PLASTID TRANSCRIPTIONALLY ACTIVE 10 / DNA-directed RNA polymerase, subunit beta'' / MurE/MurF, N-terminal / UDP-3-O-acyl N-acetylglucosamine deacetylase / UDP-3-O-acyl N-acetylglucosamine deacetylase, C-terminal / UDP-3-O-acyl N-acetylglucosamine deacetylase, N-terminal / UDP-3-O-acyl N-acetylglycosamine deacetylase / : / Mur ligase, C-terminal / Mur ligase, C-terminal domain superfamily / Mur ligase, glutamate ligase domain / Mur ligase, central / Mur-like, catalytic domain superfamily / Branched-chain-amino-acid aminotransferase-like, N-terminal / Mur ligase middle domain / Aminotransferase class IV / Aminotransferase-like, PLP-dependent enzymes / Branched-chain-amino-acid aminotransferase-like, C-terminal / Amino-transferase class IV / SAP domain superfamily / SAP motif profile. / SAP domain / Putative DNA-binding (bihelical) motif predicted to be involved in chromosomal organisation / SAP domain / Glycoside hydrolase-type carbohydrate-binding / START-like domain superfamily / Galactose mutarotase-like domain superfamily / SET domain superfamily / ATP-grasp fold / ATP-grasp fold profile. / S1 domain profile. / S1 domain / DNA-directed RNA polymerase, subunit beta-prime / RNA polymerase Rpb1, domain 3 superfamily / RNA polymerase Rpb2, domain 2 / RNA polymerase, beta subunit, protrusion / RNA polymerase Rpb2, domain 2 / RNA polymerase beta subunit / RNA polymerase Rpb1, domain 3 / RNA polymerase Rpb1, domain 3 / RNA polymerase Rpb1, domain 5 / RNA polymerase Rpb1, domain 4 / RNA polymerase Rpb1, domain 5 / RNA polymerase Rpb1, domain 4 / RNA polymerase Rpb1, funnel domain superfamily / RNA polymerase, beta subunit, conserved site / RNA polymerase Rpb2, domain 7 / RNA polymerase Rpb2, domain 3 / RNA polymerase Rpb2, OB-fold / RNA polymerase Rpb2, domain 7 / RNA polymerase Rpb2, domain 3 / RNA polymerases beta chain signature. / DNA-directed RNA polymerase, subunit 2, hybrid-binding domain / DNA-directed RNA polymerase, subunit 2 / DNA-directed RNA polymerase, subunit 2, hybrid-binding domain superfamily / RNA polymerase Rpb2, domain 6 / Tetratricopeptide-like helical domain superfamily / Ribosomal protein S5 domain 2-type fold / Nucleic acid-binding, OB-fold

Component:

: / : / Uncharacterized protein / UDP-3-O-acyl-N-acetylglucosamine deacetylase / SAP domain-containing protein / Uncharacterized protein / Mur ligase central domain-containing protein / Rubisco LSMT substrate-binding domain-containing protein / Coenzyme Q-binding protein COQ10 START domain-containing protein / S1 motif domain-containing protein / Uncharacterized protein / Glucose-6-phosphate 1-epimerase / Uncharacterized protein / Uncharacterized protein / DNA-directed RNA polymerase subunit beta'' / DNA-directed RNA polymerase subunit beta N-terminal section / DNA-directed RNA polymerase subunit beta C-terminal section

• Biological species: Chlamydomonas reinhardtii (plant)
• Method: single particle reconstruction / cryo EM / Resolution: 2.65 Å
• Authors: Kumar A / Schuller S / Schuller J

Funding support

European Union, 1 items

Organization	Grant number	Country
European Research Council (ERC)		European Union

• Citation: Journal: To Be Published; Title: Cryo-EM single particle structure of the Plastid-Encoded RNA Polymerase (PEP)from Chlamydomonas reinhardtii.; Authors: Schuller S / Kumar A / Schuller J

History

Deposition	Feb 15, 2026	-
Header (metadata) release	Oct 7, 2026	-
Map release	Oct 7, 2026	-
Update	Oct 7, 2026	-
Current status	Oct 7, 2026	Processing site: PDBe / Status: Released

Map

• File: Download / File: emd_56766.map.gz / Format: CCP4 / Size: 775.5 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
• Annotation: Cryo-EM single particle structure of the Plastid-Encoded RNA Polymerase (PEP)from Chlamydomonas reinhardtii.
• Voxel size: X=Y=Z: 0.95 Å

Density

Contour Level	By AUTHOR: 0.235
Minimum - Maximum	0.093070015 - 0.62264305
Average (Standard dev.)	0.20098166 (±0.0078017986)

• Symmetry: Space group: 1

Details

EMDB XML:

Map geometry	Axis order X Y Z Origin 0 0 0 Dimensions 588 588 588 Spacing 588 588 588
Cell	A=B=C: 558.6 Å α=β=γ: 90.0 °

Supplemental data

Sample components

Entire : Plastid-Encoded RNA Polymerase (PEP) from Chlamydomonas reinhardtii

• Entire: Name: Plastid-Encoded RNA Polymerase (PEP) from Chlamydomonas reinhardtii

Components

• Complex: Plastid-Encoded RNA Polymerase (PEP) from Chlamydomonas reinhardtii
  • Protein or peptide: DNA-directed RNA polymerase
  • Protein or peptide: DNA-directed RNA polymerase subunit beta N-terminal section
  • Protein or peptide: DNA-directed RNA polymerase subunit beta C-terminal section
  • Protein or peptide: DNA-directed RNA polymerase subunit beta''
  • Protein or peptide: DNA-directed RNA polymerase subunit
  • Protein or peptide: Glucose-6-phosphate 1-epimerase
  • Protein or peptide: Rubisco LSMT substrate-binding domain-containing protein
  • Protein or peptide: UDP-3-O-acyl-N-acetylglucosamine deacetylase
  • Protein or peptide: Uncharacterized protein A0A2K3DRS4
  • Protein or peptide: SAP domain-containing protein
  • Protein or peptide: Uncharacterized protein A0A2K3D3G3
  • Protein or peptide: Mur ligase central domain-containing protein
  • Protein or peptide: Coenzyme Q-binding protein COQ10 START domain-containing protein
  • Protein or peptide: S1 motif domain-containing protein
  • Protein or peptide: Uncharacterized protein
  • Protein or peptide: Uncharacterized protein
  • Protein or peptide: Uncharacterized protein

Supramolecule #1: Plastid-Encoded RNA Polymerase (PEP) from Chlamydomonas reinhardtii

• Supramolecule: Name: Plastid-Encoded RNA Polymerase (PEP) from Chlamydomonas reinhardtii; type: complex / ID: 1 / Parent: 0 / Macromolecule list: all
• Source (natural): Organism: Chlamydomonas reinhardtii (plant)
• Molecular weight: Theoretical: 2 MDa

Macromolecule #1: DNA-directed RNA polymerase

• Macromolecule: Name: DNA-directed RNA polymerase / type: protein_or_peptide / ID: 1 / Number of copies: 2 / Enantiomer: LEVO / EC number: DNA-directed RNA polymerase
• Source (natural): Organism: Chlamydomonas reinhardtii (plant)
• Molecular weight: Theoretical: 83.315547 KDa

Sequence

String:

MTIYPNLKKI MTKTQSTDFF IACKESRIEN NTNFYGCFYL GPFDESLSQT LANDLRRTLL SELTGLAITS IEIEGVLHKF STLTGMKEP VLDLICNLQN IVLRKETISS TNMNYRATKK TYIGFLSVNG PRVIKAADLK LPAGLQCVDP NQYIATLAED G FLNMKFNI NEGKNYIKQK PYNLDVTTLK KRNILLQNFK NKIGLTALKN KQLMSTTMEG KPLLSNSDNL RFKKSFKRML TS PNQSLKN KTSLGHDTVS NPIPLDAVFM PVTKINCIIE ENNVYSDFST DPSLEFSTHL VPSLTTQTNL RQESTHKFVN KAN SLLQEN NLFRSEKVYL PNIYIPEGEG DALSLKGVSP YSDFKTFLST LNYNSLYQTS LFLNQSLGQN KLLPWQANTL FFDV TNFAD SQSNDVNMNM DLAGDKTSVQ KIHSTGISNT DAQLNKLSLT KIKTFLGSSD KLQNKTYQSF LQPKYASSNL RTLLT QRSL QKNQSVFMHS FLDDQAKHKE LTSNTLRANK FQVMKTVVSI PPKTFKTNPI NTFHNQSLTF EYAKNFKLKP LRKKSQ LIL EIWTNGSIHP RKALYQALIF LSNNFLKLQT VKMLGSMFKS DLAYGNLKHS ITNNYTYYQT NNLSQQSKKT LFKKAEA AA SKQALNFLKP SILNTNNLPG GTMDLYLQSS LNAPIGILKI SLRSYTALKK AGITTLKDLI KYSKKDLFAI KNLGQKSV F EIEQNLALLG LTLVTD

UniProtKB: UNIPROTKB: A0A218N9C3

Macromolecule #2: DNA-directed RNA polymerase subunit beta N-terminal section

• Macromolecule: Name: DNA-directed RNA polymerase subunit beta N-terminal section; type: protein_or_peptide / ID: 2 / Number of copies: 1 / Enantiomer: LEVO / EC number: DNA-directed RNA polymerase
• Source (natural): Organism: Chlamydomonas reinhardtii (plant)
• Molecular weight: Theoretical: 93.313695 KDa

Sequence

String:

MLNISTNMMI NQTLNFDSNQ ESQKNKTERI SKTKLTRELI TKTDHDIYVD DISFLQREDN VNVSQIDLTA RGVLNPEDQG VLHKSTEFG VFNKNKTGLP ASVDLKIKQN SFFSVKQPDF NKYLISDFVE IQRNSFFTLL EKGIIEEFSK RNPITNSKKT M EIFFYPDY YQLTPPEYSP SQAIIKSKSY TSKLYIPVQL TDKSKNIIKL KWVYIGDIPL MTKRGHFILN GCARVIVNQM VR SPGIYYQ KKIYENFSNK WSEKPENTFT RYFADLICNR GTWLRIEMDK YNKIWAQMKR VPKIPIMWFL IAVGLTDKIV LKT VMDSKI LLYNLDEDPL NPRKKPLPYV KTPPAAWSAI YNIVFAKKIK AQEKAKKMLE LTEGNPSSKS QTKNKTSASK KSKT LNVAN TKGKTPAENI KTLSELIDLE KALFLKSEQG RKWIFNKFMN PRTYDLGKVG RVNFNRKLKL SISQDITTLT PQDLL AATN NLILVSKGLR ELDDIDHLKN RRVRTSGELI QIQIGVGLVR LEKTIREKMT YASGLSSLPS QKFAFRSSKQ RNQSPV GDA ENATQLTIGN LINTKPFNGA LREFFGTSPL SQFMDQINPL AELTHKRRLS SMGPGGVTRD SATLAIRGIH PSHYGRI CP VETPEGKNTG LVNSLTAYAR VNAAGYIETP FYRVYKGQVQ KKTGLYFFSA KQEEKIKLGA PDLYTSEIGF LPKASIPV R IVEDFTKISR NEIQYVGVAP IQMISIATSL IPFFEHDDAN RALMGSNMQR QAVPILKPQR PIVGTGLEAR AVSDSGHVI TAKSSGIVMY TSSKEIIIYS LQ

UniProtKB: DNA-directed RNA polymerase subunit beta N-terminal section

Macromolecule #3: DNA-directed RNA polymerase subunit beta C-terminal section

• Macromolecule: Name: DNA-directed RNA polymerase subunit beta C-terminal section; type: protein_or_peptide / ID: 3 / Number of copies: 1 / Enantiomer: LEVO / EC number: DNA-directed RNA polymerase
• Source (natural): Organism: Chlamydomonas reinhardtii (plant)
• Molecular weight: Theoretical: 70.525086 KDa

Sequence

String:

MTYSILTESI YNFKKKSSLD MQFLSPKNFQ IENFTKIHDQ TLPLKPFKTN RTAVAVVPPL PIFSPELYKR QNKLKKTQNA TIFSMGTSA FKNQVKYNLE TYHRSNQDTC LIHKPAVKEG DWVEVGDLLA DSASSIGGEL AIGHNIIVAY MPWEGYNYED A ILINERLV YEDIYTSIHI ERYEVTTKET KLGFEQITRE IPDISENEIK HLDKTGIAKI GSWVEEGDIL VGKITPFNIK TL TPQQKLL YKIFDKQLST TKDSSLRAPK GIKANVININ ILARQKIQIN TKSKNTGKGS KPPRASKAQN TMVSQPSYIH IYL AEKRKM QVGDKMAGRH GNKGIVSRIL PRQDMPFLPD GAAVDIVLNP LGVPSRMNVG QIYECLLGLA GRYLGEHYKI PPFD EMYGA DASRSFVLSK LYEARKKTGL KWLLDPNHPG KIRLFDGRNS ECFDQTVTVG IAYVLKLVHM VDDKMHARST GPYSL VTQQ PLRGRSKQGG QRLGEMEVWA IEGYGAAFVL SEMLTIKSDD MTGRQNLWKN LIENKEISLG SPESFKVLIC ELQALC LDI GLFRKNKETS LPYFKMPGEE SKTNVNANLT GVQSSKKLEA NSQTVKNSSM PNLVEIDNLL NLA

UniProtKB: DNA-directed RNA polymerase subunit beta C-terminal section

Macromolecule #4: DNA-directed RNA polymerase subunit beta''

• Macromolecule: Name: DNA-directed RNA polymerase subunit beta'' / type: protein_or_peptide / ID: 4 / Number of copies: 1 / Enantiomer: LEVO / EC number: DNA-directed RNA polymerase
• Source (natural): Organism: Chlamydomonas reinhardtii (plant)
• Molecular weight: Theoretical: 358.3275 KDa

Sequence

String:

MNIFFQRKLV KYFGNIKKKK HYLTVSEECN HYVFVKKTYV SSVFLKSLNK VNKTCNTNSG ILYNQYSLGY INMHKQLPSL IRNKSKTIG NYGVAHPSRL VKCLVTKNAT APFFNFTFDK GRLKNLVSWT LENYGQYKTV ELLEQLKKTG FEYATKAGIS L GLDDLKIP PKKKILLLEA EQLTKLTIHQ YQRGDITAVE RFQRLIDTWH RTSEQLKQEV INYFEETDIL NPVYMMAFSG AR GNISQVR QLVGMRGLMS DPQGQIIDFP IQSNFREGLT LTEYIISSYG ARKGIVDTAL RTANAGYLTR RLVDVAQHVI ISH YDCGTH KGIFLTDMKE GNKTIVSAQS RIIGRVLARD IYKPNSTITI AKRNQEISTD VAFEIGKVTN RIFVRSALTC NTTK LLCQL CYGWSLAQGN LVSVGEAVGV IAAQSIGEPG TQLTMRTFHT GGVFSGDVSD EIRAPYNGFV YYDNKIPGIL IRTLD GKIL FLTKSEGTLI FTADPNFNKP QALTDSFLNS GHGEKEKYEI KKYKIPAYTL LFIRNGESVL QKQVLAQITM ISTKPN MRD TAELVIKAEL EGLFYAKNLQ VQKKILGPKP KFIGEGKQNV LLDPKAMEII VKARGWNFAW VLSGKRYEFP LLLKSFA SS GDLITPNTIM AKHNLQLSSP FLNVGTALQV GNSTDIPKLP LLGATSKFRA MSGVLTSSIV RRYPTKINSS SRPKVNQF G KSNLFYTQVL QNKVNALNGE VDSYSETAKL PYWKNLNLKF VQKLNLSKFF KQYNLKNKTK TKLAFYSLVQ ANALQASKL DSFKANTFQS KQLGRPIFIY RHCLLTKESN KKTVKLIHNI QLQQSVLFLK IKKIKFYKMG YFQLVNSNKT AFLVSLSHNK NRLTLYHNK FNYNNSYNDI IVLPTSLTQD ISSKKQIALK RFKPAYNLFQ WFYPSLIKPS SKEAQNLTVQ QVEFFNAREQ M HFNSRYSK TDFVQLLQNP FKLDFNKALH RPLCISESYK EIPTGEVSIS PQNSVNLPMN TFFNTDGVPD KKPNYTNMYA FW LKQQVLK SYKKRYKKYK LTSILKSHLS DKIYLPASVE LQHQSQQNNL LSLTKEFKAL NSSKYLKNNQ LQSRPLLYKQ EKL LKTLVL KKWFKSNLLY VNSAISKEEN MDGSSTQMSA HSLPKRQRKG AKVSKINRFL KIDNYIKQLT YFKTSKIMRI KHSF NSLRV DFKFTSSKLV CPKRKRNLQI VCTLRGEEQT SVRESSVTHV NNLICSSKYK RSIRLQNDSH LFTTTKKAKV PFTGY SDSH QAGKVLQSSQ ISSTKPKTQS SFTFFEYFKL KLAQSGTKAI LKHFKSLKAV NISKVSMDQS RNKGFNNWGK SSSECL NTS LGDKQALYFK TKTEANLQLL TLVLENFYRK KQFANLLAKN LTILNKNLKL KKNHVHFLLY YYNKLSVQKP NAIFLLT HM LAKANKIQIS YSYVVPTSPK SKLNLDRGNL TTTNQKNVAS LGGYQPRSAG TKYYSEGIRN RTRKNNKSSM TKITKIFN Q LKLDTQFVII LLSPHKLLHH SHQLPQNTLG IYDKFNSNFE MAKVTLLTHP LWFNQFQMKS IKEVGNKFIN TRNNSLLEN YVILLLSNNY VFNFLSIKSE QINLPEEQES NLSLQEYKSP ANCQEKALPS DNKKIIFDLK KLQHIVLTEQ YKNNLRWVKN KKPRFLPKD IDINTLEVNL DRIKPKNKLS NLNPTQQLQL SNSLLEFVKT IKINPTDLAK VYSGSANTVM SIENCQPSLE V SNTFFLKT QKLLKLINKT KHSIALNQTK FINTSLLKGH LVAYARPVFI ITNKAEPFIA KQKKDLLLLP KNATINVLIK PQ QEKNSLN KAIFPLGGNA ANNHSIFVSP NTNKLANPNV SYLKKHIYFN QMPFFDSPFR NASYLFSYTE NSIKPLTKVD FMH SFAQKN HKLLPESERE KRLQFKALHI PKQPCLNICF KSNSNLIFNS KTTNSLVIRK TTTNYKYNID LSEGVDFKKL KTNM FSARK CNNFKLDTAL ESKLFKGRPT LLNYKNVVTQ TNYFSPFEGE LLATKTYKNY LDLNPYQSLK VGLMQLNTSS ALATL VPKT KRAGNKSSKQ KIKLNQGELS TELGSTIPQS GKHKTKTEKM RMAMFKYYLK TINSQKIIGN KGWSRFNLIL TKKDFI TLK YNNTLYPNFT IFSEIQKHWQ PRIQMTKPIR PISYDEVCLN YLFSEEITNQ VKLQTLAKLN KEIHFNKSYH FNLKNRW LK QKLVINASTS KSTSSLLTNI HMDQGEDKKT SVGVSLKLPA IYLCEEEGLH TNLFIHKAQR LLYKIKIILS EKALNVEH Y SWNKNSSLQK TFGYQNGIIQ AKSLLHTLPT NLNYNLKWIN YKQKNIFTTN KVGFFFLKGN TFFNTSQKLF NKKITKQTT FLNATIYNFG RNNKNNLISY NNSNFLENTH FVDLSLCLEL QKIYLNKNYL NLKPILDKTI HCQKPTKVLF KKSGFSKKQH YYLEFLNTK NHRRLIGLKE FNDYHMSYSK SQTKEMSNFI DSYYFVKPIN MDCAHYIKHE LVLYNDLITH FASLNLYISR E HGLKSLSA FFINILKIFI TSNQSQISLA PIGIDKYTNI YIPEGEGEKD MTKNVFQVIK KSGQLIQMNK EKMTLRLGQP LV ISPRSTI HATHGDFIRY KTPVVTLTYQ QLKTGDIVQG IPKIEQLFEA RTTKRGRLFR DNVTNLLTGL FLKYFIKSTY LLR KTMIGF SKKRWKKSIK YTLPVNKQPN MPRVNHTLNT TVGTELGRQS KTKVDKNKHS IAINKNLNYS NFINNKQNQT IILA LALQW AVKQSFYKIQ QIIVDGILRV YRSQGVSIAD KHVEIVVKQM TSKVRIINSN ASKMSEYMFS LDTIKAGEMP ETDLP EEEV SLQQNKAVSK QNVVAQTGKK RKKRLRKSKL SERDVITTKR TEGIDSSKIP SSNIPEGKVT QNNKRKSTRK NVSLAD REL KTRNTLSNTT KPIQISQVFE HKVLNQLLSN NLDGPTGLFP GEIVDIDFVE NINTFLLKTA SVDRASRETL STDPLNP NN QVAFAIEPIK YEPIVLGITR ASLEVESFLS AASFQQTTRV LSQAALYKKK DFLKGLKENI IIGNLIPAGT GFLSSLNI

UniProtKB: DNA-directed RNA polymerase subunit beta''

Macromolecule #5: DNA-directed RNA polymerase subunit

• Macromolecule: Name: DNA-directed RNA polymerase subunit / type: protein_or_peptide / ID: 5 / Number of copies: 1 / Enantiomer: LEVO / EC number: DNA-directed RNA polymerase
• Source (natural): Organism: Chlamydomonas reinhardtii (plant)
• Molecular weight: Theoretical: 221.076719 KDa

Sequence

String:

MHYKKYAELL KNGTYFSHIH SPTLLYPKGK VMYAQYGNSP LQGLNSQVTN LNLIRKVVAA SAEMDSFKKL PNLQNNKLPL NNLKARLNT KAIYISNEEA QLTVSKIGTP NAIETDVPGT IKNAPSNTLL THRSFKFFVN KIYAKPLISL KKTKETKPLL L GKTGTTLT QKGLNPFQSL FLNTKSSSPS TARSFGTKNI VNTLQIKKIV HKFENSYSKL TEINLITINL ASANRIRQWA EK TLPNGKV VGEVINPETI HYKTLKPIKG GLFCERIFGP LKDHECACGK KFNIKNYLTK TVNNSSIQPI AESRQTQPNT QLS LNLQKR YFCRICDVEY TYSIIRRTQL GYIQLASPTT HVWFVKGIPS YISILLDMKK KHLQGITYNT ETLTLENSFR GRQL LPVSP SSIFESWQKI MKKQYPEKYN LTNTMIKIKS TNTLPLRVSQ PNSNTSYSYM PNIYIPEGEG EEKTKTKLKK TTPLN AIAQ KGVKYKKPKT KKALYKQYLK NYYARKPKNS NQAEAVSFGV NKVQLPLTAF QQRNKVYFYK SEKRNWPVLT TVAKMQ YIV SKKGWFKLIQ YVIKSAESYG AATPTKMDGL QEYKHFSKGI KTNILITVNH NLHFLNCLLS LRSPQFVKKY LKDLDSQ TP NVGLISINTI LNFSSAQLPE IYKNELLKHL KMQTKSLVTP LYLLILAKDR GSSPLHLQKT NVSVSNLEDN SLNTTKFN S QISLSPFKLL NAPSTLLTNL SLKLDNVLIK LYNNQLSQSV NSNKVFLQNV VKLINKSSRS HIKLTHSNGL KLSKTFNHQ LWYTKIKNQK QLNPLFILTT ILKGYTFNLI EPNLNKIALM LVLNLININS IKADKMLIHN QTNRNALNST ITTKLSKKIK IWPLLTPNV KTKVLLKFFI YTTFTQSVGF IKEFNFFKNK KTYQCIKSIK NYVKLPCYNI ESDLVGEINA KSTNLKGKNL F GSLYYCSS LKMLKIFTLY FFFLKKTKKQ LIYNLYNSVL ADLKKLKNKY FKYLILSFTL FSYKKHLNLI SPLSIFLLQL TQ NTALQKL VNKTNIIFSF NVNPFHNINM INDTNLSSMA VLYPSQSIKL LCTKFDGVKT QNSVWINRVI LLATQDLDRY TGK RLISIK GSNMYLMGLT DTLIKVKMAS KLAFSKLTDE MSLPPLTTLQ LSIYTPQVEK SNNINVRLNI WHSKIGRQPD FIET ASQTK NLIVNNIYCL SHRELWEQDK DWQDFAYYYY SPGRINDKLI PLYKSRNYDS LFSMDYTLAN TPQTAIYSLG MNINT AFSG AGLIQKLLND FNFTELKKMD KQNRILLFQY NKHVQKLKKV MGTSSKGINT GNRKSRYQYY KACHIRDVLI RRTKLT RKI FNKLIPNDNF KTVDGQLTLK LVSSKGTSSA AETTKNVSVV TKIFNVSKLQ ALQNTTETNG LNMILTLLPV LPPVLRP VL KMSGQFTISD LNRLYQRIIY RNERLKKFLK DPALSSSFEM KYAQRLLQEA VDNLIQNGKS GVVPEKDARG RLLKSLSD I LKGKQGRFRQ YLLGKRVDYS GRSVIVVGPR LRLHECGIPK EMALVLYSPF LIKRILNEKL ADTYLSAKKL IRTNPLLVS QLLREIMKSC PVLLNRAPTL HRLGFQAFQP KLVDGKAILL HPLVCPAFNA DFDGDQMAVH VPITFEARAE AWKLMLARNN LLSPATGEP LILPSQDMVL GCYYLTTNCA EKWSKLKKGS GMYFHNIHEV LKAYNQHLIH LHAVIWVNIQ GHLETANNIE Q PLEIRIPL NQKYFYSQKG SYTALAGRPW QLLLREDAYS SNGLIAEANA GNLPQINVAE GEVNKNILDL RYLNLNYMEI YS KTHNLLN VNHSTLVAQR GQIKDNIHLP QLTTQIIRTT PGKILFNIIV KNAIEKRPKL LSKSNFKGGF IKNKLVNAID QEI DNYFIR KNTALS

UniProtKB: UNIPROTKB: A0A218N8D6

Macromolecule #6: Glucose-6-phosphate 1-epimerase

• Macromolecule: Name: Glucose-6-phosphate 1-epimerase / type: protein_or_peptide / ID: 6 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Chlamydomonas reinhardtii (plant)
• Molecular weight: Theoretical: 69.851352 KDa

Sequence

String:

MATSRVQASL EASSSGRGIV FVNSSVRQPK LRANGGQAAF APVTRVVSAG QRGLSSALQP PVATPQAGQP RSTQLQAVKE RTKAPKALT VADVDLEKLN EDWGIPGVVE FGEGQGGLPT VLLTHPQTNQ KLVVYLYGAT IAQWLKTDGT ATFFDGPDLL Y AEGVPLRT GVSVHFPQHR DGLLPQHGFA DRMVWEVIGA GVDSPEVAEQ LDGYLNHLQE SGLISQEMLD AAAEGGEGSE AA AELQAAL AQELEGANIA PHMLGDAAPF VMLRLRDTPA TRKMWPHRFE LCYKITLQTQ EEATDADMRD VRASLGMAPG GLA PEQDAA LAQAALEAAV MKRRAELEAE GQAAEDALAN AASGRDRRAD EAGEDEEAEG AEDQAPSDAA GKRRGRPRKT AEPA AAAAK DGEVVDGDED DDEDVSYAPV PLGEPGAVGP EPYSPPVQIK QQFWLRNRDK RGSEALRFQL ASNARFVTAQ QPDCS DWIK VLGLGGSQVI DYTADPRYPE LDLFEQDYLH YRGQPEDKTF VGAADATLYL CPGNRTHFEF IQKGGFKDMF VEQPEY DDE QPETGRLACI GTGYVASMKK LAPGKVWYAE SAIRFHNRYW TPPIFGDDTM PPLPPIPTEL LEGEGEEGDA FDDIPGG PG GDEPEN

UniProtKB: Glucose-6-phosphate 1-epimerase

Macromolecule #7: Rubisco LSMT substrate-binding domain-containing protein

• Macromolecule: Name: Rubisco LSMT substrate-binding domain-containing protein; type: protein_or_peptide / ID: 7 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Chlamydomonas reinhardtii (plant)
• Molecular weight: Theoretical: 89.099086 KDa

Sequence

String:

MLHRTRVPAT AATASGKATQ LPARVIVPCA SEQQQSLAPS PSPHARGAGS VACRARRVDN DIAGMDLEER LERLDQISGR VGRLRSVYD RQSLLEQMGA LDPALYQEET KDPDEEDDGE ELVWIPYNSD PTTHNEIRAV EEPVPGGLGT HMVTTAGVDE D EAIIQVPL HNCLLLYICE GLQTTDTLER QEAAADFQSM QATFLERWCE FHGPLPEALV AWLMEFGLQA PNARAKMAVW LL WLDACCE SPYWKQALAA LPQEADMPNL EFAADEELAQ LQWAPLAIPV GVRREALREF YAAFCRSPLA ADMGFEVAGI DAG AVSAAS GGEESGSDSD QPSTSGRGGS GSAGKGSSGR GKGRGTKGRT TAEPVAAAAP AAAPAKRPLP PFERFLWAYC HAEA RSMAQ GERIVFFPQA SPVLYTTEPS ATNTTIAFVA QDGPILDEWA VVAALKPLGP GAVLGASAPF LAGPARGGGG STTQL LEQF GVLAPARTGG NAADLIDLQP DIGSLTDVVY KQAFGQEAAT RLDMVTDPRV RHILSLIKTN DAEKEQMPFV KDILPA LQV SRLWGPGLIS GVQRLRLVEV RGEVPPEDLE AVREEMYEEE RVAEEVARIR AEAAAAAGDA PSPGPPPVEY DEFESWR QD PSLTELREVP GDLELIKTAA GRAEWRRMLA AWHSIPRAPA NTREHGRLMD LAVAGAPVHK VFSSPRDITR ELAAADAL L ADIAKLLASF PTTAEQDQQL LAAAGAGAAS GAARGRARSG RRTTASGSDS DGEGRSAAGT GAITSVRQQS IVSWRLEFK LVWGQMQGMV QEYRDALAAA EKAAEKEAGA LAAV

UniProtKB: Rubisco LSMT substrate-binding domain-containing protein

Macromolecule #8: UDP-3-O-acyl-N-acetylglucosamine deacetylase

• Macromolecule: Name: UDP-3-O-acyl-N-acetylglucosamine deacetylase / type: protein_or_peptide / ID: 8 / Number of copies: 2 / Enantiomer: LEVO / EC number: UDP-3-O-acyl-N-acetylglucosamine deacetylase
• Source (natural): Organism: Chlamydomonas reinhardtii (plant)
• Molecular weight: Theoretical: 66.248688 KDa

Sequence

String:

MNSTLNRRLA QTSTGVALLP CPRLHRLQTV SHSPIQAAAP CPARAAEQLP SLTSSRAVAA AAGRQAEAAS ATNDRKRTRL AADKSPELI DPQLLAPIPM PTEYQQTLWA SFTIAGVGLH SGEYAVVRVR PAFAGEGRYF VRVPEGTNAA RGEESLVRML E AGAGKQPE EEEAMDDTAT REYKRELIAA YQEYVDAQED DGFNGTFADY MNGKSYVALL QRLREEGPPR FSFSEAEAPQ PR GEDEPYV EAALANITEP NPFTTALAKG DVEVLGVEAL LSALEACGVD NARIEIEGGA EVPVIDGSAG GWASQIQTSG LTH ALPRDD LPVPGGEFPA PADGEEAAAG DAEARKQRVC KMVLRPQEVV SVQRGASFVT LYPEDTFRLT VGLDRHLEAP VIGK QWFSW CMFEDLHYMH SLADARSWVS SPQQAMAMRD AGWLLGGTEG AMLIGNGDSW YDHTIVRHPR DEPARHQMQD LIGDL SLLA RHGHSGLPLG HVVAYDADHE LFAAFVERLQ ELTTEEDWVP IQDIAGPSVA TDYEELLGSL LEGDEGEDDE GEGGEG AQL ADQGAEEDED EVYDEYEEDE GEGEEYDEYE EEGEEAPGPE AGRARR

UniProtKB: UDP-3-O-acyl-N-acetylglucosamine deacetylase

Macromolecule #9: Uncharacterized protein A0A2K3DRS4

• Macromolecule: Name: Uncharacterized protein A0A2K3DRS4 / type: protein_or_peptide / ID: 9 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Chlamydomonas reinhardtii (plant)
• Molecular weight: Theoretical: 38.135762 KDa

Sequence

String:

MPAARSLDSS ECSTSYSAQH YRRTSRGERM RTCAAANDDD FKPAGAARRG RGKARAPPPP PPPPPQQGAE DEEEYEESLG ASLEDPAQG QLQRLLSRSG PGESVAADVG PAIKRPPTHP LDMYETLYGD QDSDWRDLPL GLLPRSADEE RKLMAKLQVE A YFMRQWNH EDVDDLAFFE AEKMRGKANN IYANVRRVAS MMNVIEAEDF AMAERQSYFT PWARAEIAFA SEYDDFNAAV KA NIDSLVR QPLEEELRER LEAIGEATLL PPTEAEQQAP KFPSDADVSA YMMENFLEGV LPGVSLTNPN QRYADDIYGG DEE GADELL NMVEEQEGDG EAEPADE

UniProtKB: Uncharacterized protein

Macromolecule #10: SAP domain-containing protein

• Macromolecule: Name: SAP domain-containing protein / type: protein_or_peptide / ID: 10 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Chlamydomonas reinhardtii (plant)
• Molecular weight: Theoretical: 280.466875 KDa

Sequence

String:

MLHVGQASAG RPVASSRSVA RGPRPLQCPV STFAPSSRRL IAEAKRGGRP TSEPSAAGDD ADPLAAVAEL NFRELQAELK ARGLPAGGK REELAERLAA ALADEGAGAG ASATGGSSSS SGSGSGNAKA AAGIDLDSAL DLLEEVPYAE LKALCAEAGL S AAGKKAEL QQRLAEAAVA GDIDLVSPLE GEDLEQPEAA RAASHGSAQS SAAAAADAAA LLPELLALSA EELEASLSEY GV AAPGGGG GRGWRGDKRA MAQALAEAMV AEVTAELEEG GEAADEDELQ EGGYDDDADG PDALFDAEME LGDADRKRLL AAE SEALRA QLGGSTRQQL YAALKELDRH ATYTTRGVLL DRLVRARAEA ALEATLQAAA KSPDAAAKLR KRIARAGSSS TPDA AGDAG AGAAGVAIDF SKLTLPDLKH MRVTDLRAAL EHFGMDSNGN KYDMLERLKA HLVALQPGGV IRRGRTPAAV TAAAA AAAA APEEGGGSAA EGSAAGGGAE GGEGEPSLDY VLGMNLRSLR EELDKRGLST AGARTQLQQR LMLELRAEAE RSGRGA GGA GEAGGLADDD HDEEVLEGGL PVRLAALAEL PLERRAAAAV AAAACDPARL LVLGGEAPVD VAVVAGGCPP GRLDAAA AA LEAARAALDA LYTAHMDSPL DAATRPDPAA VSRFAARAAE VGAAAAAAEL EAAAAAAGDA GADSAQLLTG VTAVSAAP A AGGVPRAATG TVVSVWWLDG ASGAATLLSP AQVYAATPAE LAAGCLPGVH PGLLAVPPPS PLSLSTPALD VPVPAVASA ASSKAGVPTT YDSVAALAKH LKAAAHVVFP AVPAGGRAHC ELAAALEDAG MGAVVGSGAE AAALTTDRVA LLAKLSELNY PVSPLLHLT AADVAAAVAA ARAEAAAAAA KAKAGAGGKR GRKTAEPEEA EASEEAAEEE ADAEEVAVLR AAYAAVLAKV T AWFEQQAY HPEQQLVILR PRHSGGLATA ADAATAMGAA SAAALATQPL VAAAEGGAAA AALGAWTDMV LEAVPSPPAA AA AASSSPS SSGSATDMGS QSSVGKSGTV ARFVCTVVET PDGPVALLPT ELQVVDTDAA IRVHAAEVAE WEALKAGASD AEA AAAAAA AAAEEPEVWR LLGADPRSRV LPASLPSTVS ARLHTPPRFS RKLAHAVRHA AAKLFGQLGL RDVATVEGWA LLPH GYEAA LQAVDEDGEP VVPVLWDEDP AILQRLAQQE AERIAADPSP LEAYYDAGSS WHADLDAFEP ARYLSAASNG DHGAG VLLS AVDAAPPVSR SHPAVLAAAE LGLPHAALLR NLANSALRRA AAAGDARALT PGGEYVPPEE VAEAAVLSVA MRDALA GGG EAADEASVAA QAAAAEAAAA PVALAPAALQ LPMPPQLPLY HSAVLQHLRE GDEYDQWVET VDPDYWDDAE GPLRVAV AE EHMHQAAEAD SGLPRAYGDD PLPLATDMAA AVEVEGQRLL LDAAVEALGD FGAGLASAMR ALPLEDALAE DAEISLRR V RPLPPPEELL GDGSDGTADD PRIRAAWQLA AISELARRHG WELLECADAV QEERDRAAAG EAAPSAEEAQ AAAAAAESE QLVLEAMCED DDEVMELRTG MVTPVQVYLR FKARMSRREP FAHPLLLSAA TTLGYDEFVA PAGGEAQQQE EEVEAELVPA TSGKADSGR LQILPEGEDS PFGPGSTGDA SSPTHPAAYV FNVPPDEAVE LSAPMSAALL AEQLADNGAD GGLVAAAAQP I LEAAAARM GLPQPSGIAG GSSSSTRVGS RAKRSGAVAE GEVEAGEEEE EVDGEEEEEE QGGGVRASAF DASEAEALTL ME LGFRAEP VALDEASGAP LLAAVGPSIE GLHEEVVVGG WGGAFAQLPA FRPLAAILEE ANLHPGPAAA ASSSGADGEG TGA LADGGY GPEFEDDAED DEEDGGFGAA ERRALAASSS AGSGSAYAAE DEAGEEEPSS VAAVVRQVAA DTGDDAQAME LAAT AAPLA PTRVWVVVGG DAAHHAGREA GMIAGANVLA KLGRYQDLVV EPFLMVPHGE GGDAEARRSE LLRRRTQAVS GLGLT AGAD MPHYLADLEA IRSTADVANP SGRMDMQPIY AVTPATLARS SVPDALWSVE ATAAREGAAL HALSELQRQQ RAVHRD VQA ELAAAGFEGV ASMWDSNITG QPGPPPARPL DLTSFAEDAA RAGAVVLVAG RGSMAECGAL QSLLQLNGVP FVGPGPD QL ALLWDKGELE ELLADLRDSA GVQVPPRLIM PTATALVAAD LDEDEADKMM SSLRGHVMGE AAAAAGTAPL LVRPAAEV A GVGVARLDTG ADVVAYVAAL AAGAEVLPAD TLSHPHPEVR LPPLLPLELI FEPYVEADAL MLVVGPGAGS GGGSEAAAA AATAEGSGAG KRKRRTTEAK AEEEAAGAGA SAGASGAGGA RLQCLGRSGW VEVCFSLIGP LGAMSCLPPS VRAAVVAAPD LQQAEEAGA AEGGGGAAAA VAALLAHSPV APVCPPPAGV LAPEVLAAAC QRAVAVADRL ALRGVAQVEA FVSVATGELL V TDINPVPD LGPDSVIYRQ AAAAGLLPAD LLRQLLGLAI QAAAAPQAAA ASFEGQMAAL EAEAEEEGGP VRWDDVAYDE EE AAAAGVV GAGVDEEEEV EEEGGLGGLG DEEDEGEEGA VGGRRRRGGG GAEDDEDMLG QLEEMRGGVG ADAEEEGFEE DFG EEAWGG GGAASSGSRG GRIFFEEDGV GMGAGAGIGG GYMPLSEAGR GVGRGGAGGR GGARKRYDDD DE

UniProtKB: SAP domain-containing protein

Macromolecule #11: Uncharacterized protein A0A2K3D3G3

• Macromolecule: Name: Uncharacterized protein A0A2K3D3G3 / type: protein_or_peptide / ID: 11 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Chlamydomonas reinhardtii (plant)
• Molecular weight: Theoretical: 189.894359 KDa

Sequence

String:

MRLGGPSCGA SLAPIAIGWR LRGILPPTYT PSRIVCAHAA KRGRPRKTDE PAPEIAESEE PEAPAEDPAA PRRGRRSKKE KAQEEARDL LQSQQVDLEV LEEFKARAAA EDEARKAAIG GGEDDPRRLN FHRSLLAASA RGLTAECSRI IDSMIDAGLQ P GARAIHVW AYSHIVVGDG RAARRVAEEA KQTFGVEWIP ETYVALMHGA LSCNGEAPDL MAALQMWVAQ QDAAAAGRNA NP QLGFVFL AKELFRLRYS ALAMQVVSEG YAAGFKPDDK LAALVIEQLC KQGLQAEAAA EMQRLLDAGL PVGPEQYDTL VRL EAARGS VEGARSMLQN FYTDARFSPP RASTYNALLK GLASGLRLAP LGGAFPAAAL AGDEDGLDAD EEAAAAGGRR LVLD GDSLD KLLVVLREEM VARGLRPGKE TYAAMVESFA AVGDLDGALS AYDTLLRANG SPALLRKKYL GRLVVGLLVS DRPID ALRV LRDCSESSSF DDARESVTLP PAASDALPGP AAGGRTALTV WLQAHYETMR QRRGQRASVL QFMQDADMSS RREVDG VVL GLGGCVVTAD GLFVPPSRMT TAELRAELSG AGIEVPASAT GGSSSSTRSQ LLKLVKDRRT KLPSGVIEMQ AQLAAQL EA REEAELRAAR KAAALASREE SGGDDDFLWD DEAEEDEMED DDDDDGPTGL DPNVASIIAD EGTFEGGDSI EDSPTFFA A ARGRLAATGE MQERDSLRVR GAAEAEEAEE EDEEAEVEDE EELEDEYEYD DVDVDVDDDD EDLPLIRLDD LDEDEDEEE DEEEDDDADD MLESVDMISG PGLDAAATAS RNKRRRKRRV SDEDDDDYDE EDDELAELGL DDDAPLSDGL GPIPGANRRG REFIQMQKV DDDEAILDQV LAMSTFDASR FNEAAGMVVA LRMLGLWAAV GGVPSAADFL ALYEGAVVEA HPRCAADLAE Q LPVLEAGG DVSSEQLTEM MVTLANICLS PAKADAEAAD RIITAMEDAG LAVPPGLYSG LAAALGGRKR TRESVLGRSG HE VAAVEWE AAGNMANVTL DADVEDIRGA EEGEGEAGAT ASTDEAEEEA EEGEEGEGEE ELFAGYEEDP ASQEAEELEW LFQ RDADED DSWMDDLDTR PADVEEVRAE AEAKAAAARQ EAAVANALER VRLVAGPAAA ALLAQTIEQR DAARLEEAAK EAAV ASDTV AEERVDLMSS LETALVESGT VTVLPAVAAP QAWQAGGAGS GSGDGARPEE ARADAELQDL TAEVEWQAKS MLELP PVVL AEAANAALAE MRAAGVVDED DAAALQRIAD KATVLEQLRA VSTAAAAAHP GGMDAAAEDV EAALGKLQAA EAAAMS YAA AVGLDAAAAG EDLAAAAREA AGWGEDEVLD FLDEIDETRG EMDEAEAAEM LTEEDLEPAV DHETALTGVA GELAEEA VR QAEALLELEA EGGINTGDGD TPDEADERLL SEAERGYRAA LVLGLEDAEA QQAALQRAYQ GAEAALKAGK KPKWSKEQ Q AVAAALDAGD MGALQERLGL EGVEWIAGEL RALAEREEME LPAGVDEADE DEVPVLPGGL ALSERFMEAV RATVADRAA AAEAEGVPPA EVLGGELAVL DAMLALANGY RPQGFEGESV SDLAGANEDG EDGSELGRLL QQLRRDASDA MAAADAAVGT AREEQAFAA AASVVSELME VASEVYEEAE EAAAEAEEAM GEYEEGEELS EEDEAASREA ARQMLAALAA LQPNAGLPFG A DGAGAEEE QGGAATAVAA PPAPAPPKAG RKGRGTRGTA SSE

UniProtKB: Uncharacterized protein

Macromolecule #12: Mur ligase central domain-containing protein

• Macromolecule: Name: Mur ligase central domain-containing protein / type: protein_or_peptide / ID: 12 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Chlamydomonas reinhardtii (plant)
• Molecular weight: Theoretical: 86.41743 KDa

Sequence

String:

MLAQQLARSV RRPAAPPCTS SPCTICPVVV RQPANKCHAP RIAPVAPAPA RQTLCAARRG RPPGGPPEPT PEGPEPEFFE DDDINYEDE AEDGEFVEQL DPHTRRLMGI AEQREDVEEA GYEEYEEYEE AEDEGALDDA AEADRDGIAA DEDVGEPSTS Y PEPPDRAF RAPDLFGEAA LFEAALWLAA HFPNIEVTGV QSNPHLVTPG DVYVHHEGLT DEDQVAAVSV ALEQGAVLVV AP HLPKDST GLHVGEDPLE GVVPDEVPLV RVKDSTEAGS RLAAAFYETP SRNMVTVGVV GLLGKTTTSW LVRGILEELG QTV GLMSSI EHAIAEDRLD EDGYLWEPLE EDPSADRESS VPFKLIPYEG KYEQHYGRRL PGLNAQKVLA GMRDRGAGVA VVEL QADGL MLGTYDFMSL NVAVVTSLSP RPGVPAAQQP AELVAAAADE VHAAIELLSA RLVDPETQAL VLNADDPAAE QLLER LGGA VPVVTYGVVN KGADVVAESI KSNIWETELI VRTPGGRLQI ITNLLGRHNV ANVLAAVATG VALKAPLASI VAGIEA VEI PGRSEVIDEG QEFSVVVDAA RTPEALGDML DALRAGGARQ IFTVFGCSGN EDASIRPRMG AVAHAKSDYV IITNENP RL EDPAKVVADI VAGFPDDMVN RYSAYAYFPF QDQGRTPLWF EPYLQKAQRD NKRYVMEDRY SAIRAAIGTA GPEDVVLL A GKGSEDWVEH ADEDGSVLTG WLDDRVEARN ALSKLKYLKQ IPRFSRETLP WGSRIEGLME TLAVDPAELS AAAVGGLEG ALD

UniProtKB: Mur ligase central domain-containing protein

Macromolecule #13: Coenzyme Q-binding protein COQ10 START domain-containing protein

• Macromolecule: Name: Coenzyme Q-binding protein COQ10 START domain-containing protein; type: protein_or_peptide / ID: 13 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Chlamydomonas reinhardtii (plant)
• Molecular weight: Theoretical: 43.963891 KDa

Sequence

String:

MRQLSVLDSG SRPAAQPVAS CSYTTLPNFT SRRIHQRQHA CGRGADQSAG RRRLAAPAPL LAPHRLTDNS RPSIACASGE EVISGYTPA NYISFSEQPG DVAGHGFSVV VDAPSSVCFG IWNDWNRLVE FLDLVAQIGL DPKNPDMALF QTFYRHGLLP V MEIVFVLQ KTQVVENERI EFESVWGMPM SGSVSFRPVD GAGGSRTEVT LEFAQALPTL LVDLKVGVFG VQNSLRPILS DN LVAFKEL AEELAAAESS QKGKAGKGAR PRQEAGDRQF LLFDERADMQ AYAEMMEMMQ DAELQQQEQQ RQAAAAAQRA SAA ASQPQQ QEQTPAEEDT ARQQGPGTAA AAAGPAVAGS GRAGRGTRRT TRTTTDAAGG TAAGGRAASD AAAAGGGQGP DDRS SGRKR SVRSTRGAS

UniProtKB: Coenzyme Q-binding protein COQ10 START domain-containing protein

Macromolecule #14: S1 motif domain-containing protein

• Macromolecule: Name: S1 motif domain-containing protein / type: protein_or_peptide / ID: 14 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Chlamydomonas reinhardtii (plant)
• Molecular weight: Theoretical: 40.289797 KDa

Sequence

String:

MQGALRGLGA RPPTNCGGAS CSGRVGPCSA QLNPRGMVPT VGRGHQHRIL PPCRITSTQK ELFQSKEAKD MNIEDKGEDL YMDLPDPQV FDAEKGPLEK RIQRTFDQAV KDDIVDLRDT EYWFSTPEET WNSKPQRVHF MEQDEEPLTP QAWHPPQEGL P EGDERIAF TELEEGRVYL GMVTDVWLYH GVQVDIGAEF DGLIPCTEEQ WELAVDHIDV GEVVLVEVHK MRQPGLYRWP LQ LKFCVKG LQDLMTPPDE YTSPVDHGWA VDQGWSAEDI AEAAGRQLEV PSYFMPHDEQ KQAEDVIDGY GTGEMYWRSS RSE PLEDYV DDPLEDPAAE QMKRRQAEVD AAAADLLRGL

UniProtKB: S1 motif domain-containing protein

Macromolecule #15: Uncharacterized protein

• Macromolecule: Name: Uncharacterized protein / type: protein_or_peptide / ID: 15 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Chlamydomonas reinhardtii (plant)
• Molecular weight: Theoretical: 41.104062 KDa

Sequence

String:

MDCSALAARR ALSQCAGPSS RCLVPAALPQ SRPRTCSRST GHAAGHRATT IVARANGKTV TPSPLFDEVA ESEGFIRLVS PDSAEPNFD DPDWISKVDD WHEFWNYNNW ELEVEDIETE EGNIAGPMEA VRRAEKLVDA FAEMDMRTDI VNWMGTQWSE D TFEEDKYA NPPMDPERPD PNPPLTLWDL RARAEKARQR QLIDAEWHRR QNRIGKLWYP HMDEYRDMRV RDNPVEGYRH DW TEEQIQL LITNNGIACD PKTHGALTEN PLANQDYHAL GVRYLEETEE LLERTGHLAS TNLRTQFDRE FILHGEDYGE DEA EAELDA MRREAEREAL IEAGIDPDEE DPDSEVVGEV RLG

UniProtKB: Uncharacterized protein

Macromolecule #16: Uncharacterized protein

• Macromolecule: Name: Uncharacterized protein / type: protein_or_peptide / ID: 16 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Chlamydomonas reinhardtii (plant)
• Molecular weight: Theoretical: 40.748148 KDa

Sequence

String:

MQLGRQLSAH AGTCRQQALR CSSLARSAPP PAICPSRAPT QPCSRGSVQL HAKRTGGRSK TQEGSDDASG WDAALADIPT EDDEAASED FEESDAERSA GQAGEAGEPS TSYAPVGKVS SGPRAPKAPS SLLSLAEYNM LMEAKAIREA QEEAERLERE I AEGRGVVV TEPVPIDVNR QGFQVFPIDG GDLELLVAQD GQGLLALADS YVAARQPAAE GGWAYSRVKL EGEVPTGASV YT VVPWVER GGSDMFKRKA ATNKVKKVYF INMAPADDDS GEAAFNLDAV FCFDGKSRTM ALSEVRLVGQ PPLMEPVLRI RPS GLTLEE SYLLPDPIEI SMNTAVDLAD MSEEELAERL AELEAQAEAL DELDEAPDVP SVDNY

UniProtKB: Uncharacterized protein

Macromolecule #17: Uncharacterized protein

• Macromolecule: Name: Uncharacterized protein / type: protein_or_peptide / ID: 17 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Chlamydomonas reinhardtii (plant)
• Molecular weight: Theoretical: 44.891465 KDa

Sequence

String:

MEASCSSHCQ GAATSRLGQR GWRMPPARVL RLRMQASMPV EIGKLEKQPI PVPKDYKPSV VPGSRTRPTP RLDAVQMIDR LQRTLPVYV REQFGSFYSS HMGGIVTDPA LMMLPPDDQL VCKGHGVSEV VVLREGHIYL LDRHIQRLKE SCEQVGIALP F EEASLKRI LLDVAAASGR VNGVVRFWAT PGRGGFSTVE TGGAEPAFYA LCLGDTYYMD RMEAWPAMLV SQPVTPTLTS NV PGNQHLL TSVTQLIAGE EGMKATLFTD EEGFVQHGSG FTVCILTNSD VLVYPPNAHV TPSITLERIL EMIPAERARS PDD VVVREV QQRKLHISEL QAAKEAFLVC TTYTVAPLAS VDGAPIADGA TGVTTLALHY MLDNDMRPPP PGVISDRHTS VPYG YLTGM RDQLV

UniProtKB: Uncharacterized protein

Experimental details

Structure determination

• Method: cryo EM
• Processing: single particle reconstruction
• Aggregation state: particle

Sample preparation

• Buffer: pH: 7.5
• Vitrification: Cryogen name: ETHANE-PROPANE

Electron microscopy

• Microscope: TFS KRIOS
• Image recording: Film or detector model: FEI FALCON IV (4k x 4k) / Average electron dose: 60.0 e/Ų
• Electron beam: Acceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
• Electron optics: Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 2.0 µm / Nominal defocus min: 0.5 µm
• Experimental equipment: Model: Titan Krios / Image courtesy: FEI Company

Image processing

• CTF correction: Type: PHASE FLIPPING AND AMPLITUDE CORRECTION
• Startup model: Type of model: NONE
• Final reconstruction: Resolution.type: BY AUTHOR / Resolution: 2.65 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: cryoSPARC / Number images used: 650394
• Initial angle assignment: Type: ANGULAR RECONSTITUTION
• Final angle assignment: Type: ANGULAR RECONSTITUTION