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- EMDB-54786: Cryo-EM structure of the Human RUS Family Protein 1 Homodimer -

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Basic information

Entry
Database: EMDB / ID: EMD-54786
TitleCryo-EM structure of the Human RUS Family Protein 1 Homodimer
Map data
Sample
  • Complex: Homo-dimeric complex of human RUS Family Protein 1
    • Protein or peptide: RUS family member 1
  • Ligand: CHOLESTEROL HEMISUCCINATE
  • Ligand: TRISTEAROYLGLYCEROL
Keywordshomodimer / MEMBRANE PROTEIN
Function / homologyRoot UVB sensitive family / : / : / Vitamin B6 photo-protection and homoeostasis domain / Root UVB sensitive protein, C-terminal / membrane / RUS family member 1
Function and homology information
Biological speciesHomo sapiens (human)
Methodsingle particle reconstruction / cryo EM / Resolution: 3.13 Å
AuthorsLin Y / Kato T / Newstead S
Funding support United Kingdom, 2 items
OrganizationGrant numberCountry
Wellcome Trust219531/Z/19/Z United Kingdom
Wellcome Trust215519/Z/19/Z United Kingdom
CitationJournal: To Be Published
Title: Cryo-EM structure of RUSF1
Authors: Lin Y / Newstead S
History
DepositionAug 13, 2025-
Header (metadata) releaseAug 12, 2026-
Map releaseAug 12, 2026-
UpdateAug 12, 2026-
Current statusAug 12, 2026Processing site: PDBe / Status: Released

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Structure visualization

Supplemental images

emd_54786.png

Downloads & links

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Map

FileDownload / File: emd_54786.map.gz / Format: CCP4 / Size: 91.1 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Projections & slices

Image control

Size
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Others
AxesZ (Sec.)Y (Row.)X (Col.)
1.04 Å/pix.
x 288 pix.
= 299.52 Å		1.04 Å/pix.
x 288 pix.
= 299.52 Å		1.04 Å/pix.
x 288 pix.
= 299.52 Å

Surface

Projections

Slices (1/3)

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Images are generated by Spider.

Voxel sizeX=Y=Z: 1.04 Å
Density

Histogram

Histogram (log scale)
Contour LevelBy AUTHOR: 0.523
Minimum - Maximum-2.8359334 - 5.748902
Average (Standard dev.)0.0012620295 (±0.07579669)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions288288288
Spacing288288288
CellA=B=C: 299.52 Å
α=β=γ: 90.0 °

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Supplemental data

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Mask #1

Fileemd_54786_msk_1.map
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AxesZYX

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Additional map: #1

Fileemd_54786_additional_1.map
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Half map: #2

Fileemd_54786_half_map_1.map
Projections & Slices
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Histogram

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Half map: #1

Fileemd_54786_half_map_2.map
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Sample components

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Entire : Homo-dimeric complex of human RUS Family Protein 1

EntireName: Homo-dimeric complex of human RUS Family Protein 1
Components
  • Complex: Homo-dimeric complex of human RUS Family Protein 1
    • Protein or peptide: RUS family member 1
  • Ligand: CHOLESTEROL HEMISUCCINATE
  • Ligand: TRISTEAROYLGLYCEROL

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Supramolecule #1: Homo-dimeric complex of human RUS Family Protein 1

SupramoleculeName: Homo-dimeric complex of human RUS Family Protein 1 / type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1
Source (natural)Organism: Homo sapiens (human)

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Macromolecule #1: RUS family member 1

MacromoleculeName: RUS family member 1 / type: protein_or_peptide / ID: 1 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 52.306781 KDa
Recombinant expressionOrganism: Homo sapiens (human)
SequenceString: MADDAGLETP LCSEQFGSGE ARGCRAAADG SLQWEVGGWR WWGLSRAFTV KPEGRDAGEV GASGAPSPPL SGLQAVFLPQ GFPDSVSPD YLPYQLWDSV QAFASSLSGS LATQAVLLGI GVGNAKATVS AATATWLVKD STGMLGRIVF AWWKGSKLDC N AKQWRLFA ...String:
MADDAGLETP LCSEQFGSGE ARGCRAAADG SLQWEVGGWR WWGLSRAFTV KPEGRDAGEV GASGAPSPPL SGLQAVFLPQ GFPDSVSPD YLPYQLWDSV QAFASSLSGS LATQAVLLGI GVGNAKATVS AATATWLVKD STGMLGRIVF AWWKGSKLDC N AKQWRLFA DILNDVAMFL EIMAPVYPIC FTMTVSTSNL AKCIVSVAGG ATRAALTVHQ ARRNNMADVS AKDSSQETLV NL AGLLVSL LMLPLVSGCP GFSLGCFFFL TALHIYANYR AVRALVMETL NEGRLRLVLK HYLQRGEVLD PTAANRMEPL WTG FWPAPS LSLGVPLHRL VSSVFELQQL VEGHQESYLL CWDQSQNQVQ VVLNQKAGPK TILRAATHGL MLGALQGDGP LPAE LEELR NRVRAGPKKE SWVVVKETHE VLDMLFPKFL KGLQDAGWKT EKHQLEVDEW RATWLLSPEK KVLLEDYKDD DDK

UniProtKB: RUS family member 1

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Macromolecule #2: CHOLESTEROL HEMISUCCINATE

MacromoleculeName: CHOLESTEROL HEMISUCCINATE / type: ligand / ID: 2 / Number of copies: 6 / Formula: Y01
Molecular weightTheoretical: 486.726 Da
Chemical component information

ChemComp-Y01:
CHOLESTEROL HEMISUCCINATE

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Macromolecule #3: TRISTEAROYLGLYCEROL

MacromoleculeName: TRISTEAROYLGLYCEROL / type: ligand / ID: 3 / Number of copies: 2 / Formula: TGL
Molecular weightTheoretical: 891.48 Da
Chemical component information

ChemComp-TGL:
TRISTEAROYLGLYCEROL

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

BufferpH: 6
VitrificationCryogen name: ETHANE

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Electron microscopy

MicroscopeTFS KRIOS
Image recordingFilm or detector model: GATAN K3 BIOQUANTUM (6k x 4k) / Average electron dose: 42.0 e/Å2
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 0.8 µm / Nominal defocus min: 0.2 µm
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

CTF correctionType: PHASE FLIPPING AND AMPLITUDE CORRECTION
Startup modelType of model: INSILICO MODEL
Final reconstructionResolution.type: BY AUTHOR / Resolution: 3.13 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: cryoSPARC / Number images used: 284046
Initial angle assignmentType: MAXIMUM LIKELIHOOD
Final angle assignmentType: MAXIMUM LIKELIHOOD
FSC plot (resolution estimation)

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