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- EMDB-54627: Local refinement of a PSI monomer of A. marina NIES-2412 -

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Basic information

Entry
Database: EMDB / ID: EMD-54627
TitleLocal refinement of a PSI monomer of A. marina NIES-2412
Map data
Sample
  • Complex: Photosystem I
    • Protein or peptide: x 12 types
  • Ligand: x 9 types
KeywordsPhotosystem I / Chlorophyll d / long wavelength / ELECTRON TRANSPORT
Function / homology
Function and homology information


photosystem I reaction center / photosystem I / photosynthetic electron transport in photosystem I / photosystem I / plasma membrane-derived thylakoid membrane / chlorophyll binding / photosynthesis / endomembrane system / 4 iron, 4 sulfur cluster binding / electron transfer activity ...photosystem I reaction center / photosystem I / photosynthetic electron transport in photosystem I / photosystem I / plasma membrane-derived thylakoid membrane / chlorophyll binding / photosynthesis / endomembrane system / 4 iron, 4 sulfur cluster binding / electron transfer activity / oxidoreductase activity / magnesium ion binding / metal ion binding
Similarity search - Function
Photosystem I reaction center subunit PsaK / Photosystem I reaction centre subunit PsaK / Photosystem I reaction centre subunit PsaK superfamily / Photosystem I reaction center subunit V/PsaK / Photosystem I psaG / psaK / Photosystem I reaction centre subunit VIII superfamily / Photosystem I PsaF, reaction centre subunit III / Photosystem I PsaF, reaction centre subunit III superfamily / Photosystem I reaction centre subunit III / Photosystem I PsaD ...Photosystem I reaction center subunit PsaK / Photosystem I reaction centre subunit PsaK / Photosystem I reaction centre subunit PsaK superfamily / Photosystem I reaction center subunit V/PsaK / Photosystem I psaG / psaK / Photosystem I reaction centre subunit VIII superfamily / Photosystem I PsaF, reaction centre subunit III / Photosystem I PsaF, reaction centre subunit III superfamily / Photosystem I reaction centre subunit III / Photosystem I PsaD / Photosystem I, reaction centre subunit PsaD superfamily / PsaD / Photosystem I PsaE, reaction centre subunit IV / Photosystem I reaction centre subunit IV / PsaE / Photosystem I PsaJ, reaction centre subunit IX superfamily / Photosystem I PsaJ, reaction centre subunit IX / Photosystem I reaction centre subunit IX / PsaJ / Photosystem I PsaA / Photosystem I protein PsaC / Photosystem I PsaB / Photosystem I PsaA/PsaB, conserved site / Photosystem I psaA and psaB proteins signature. / : / Photosystem I PsaA/PsaB / Photosystem I PsaA/PsaB superfamily / Photosystem I psaA/psaB protein / Electron transport accessory-like domain superfamily / 4Fe-4S dicluster domain / 4Fe-4S ferredoxin, iron-sulphur binding, conserved site / 4Fe-4S ferredoxin-type iron-sulfur binding region signature. / 4Fe-4S ferredoxin-type iron-sulfur binding domain profile. / 4Fe-4S ferredoxin-type, iron-sulphur binding domain
Similarity search - Domain/homology
Photosystem I P700 chlorophyll a apoprotein A2 / Photosystem I reaction center subunit PsaK / Photosystem I P700 chlorophyll a apoprotein A1 / Photosystem I reaction center subunit IV / Photosystem I reaction center subunit II / Psa27 protein / Photosystem I reaction center subunit III / Photosystem I reaction center subunit IX / Photosystem I iron-sulfur center
Similarity search - Component
Biological speciesAcaryochloris marina (bacteria) / Acaryochloris marina NIES-2412 (bacteria)
Methodsingle particle reconstruction / cryo EM / Resolution: 2.44 Å
AuthorsConsoli G / Leong HF
Funding support Netherlands, European Union, United Kingdom, 6 items
OrganizationGrant numberCountry
Netherlands Organisation for Scientific Research (NWO)714.018.001 Netherlands
European Research Council (ERC)European Union
Biotechnology and Biological Sciences Research Council (BBSRC)BB/R001383/1 United Kingdom
Biotechnology and Biological Sciences Research Council (BBSRC)BB/V002015/1 United Kingdom
Biotechnology and Biological Sciences Research Council (BBSRC)BB/R00921X United Kingdom
Biotechnology and Biological Sciences Research Council (BBSRC)BB/Z516740/1 United Kingdom
CitationJournal: Sci Adv / Year: 2026
Title: Far-red chlorophyll d clusters extend photosystem I absorption toward the red limit.
Authors: Thomas J Oliver / Eduard Elias / Giovanni Consoli / Ho Fong Leong / Violeta Cordón-Preciado / Andrea Fantuzzi / Tanai Cardona / A William Rutherford / Roberta Croce /
Abstract: Oxygenic photosynthesis is usually limited to visible light, but the marine cyanobacterium pushes this boundary by harvesting far-red photons with chlorophyll d. The best-studied strain, MBIC11017, ...Oxygenic photosynthesis is usually limited to visible light, but the marine cyanobacterium pushes this boundary by harvesting far-red photons with chlorophyll d. The best-studied strain, MBIC11017, unexpectedly lacks low-energy chlorophylls ("red forms") in photosystem I, limiting absorption beyond 740 nanometers. Here, we show that another strain, NIES-2412, has evolved a strategy to absorb far-red photons up to 760 nanometers. Combining time-resolved fluorescence spectroscopy with cryo-electron microscopy at 2.64-angstrom resolution, we identify two distinct classes of chlorophyll d red forms in its photosystem I. One class originates from classical charge-transfer-exciton mixing, while the other arises purely from excitonic interactions. Mapping all 96 chlorophylls d reveals the precise pigments responsible for these far-red states. We also uncover a previously unreported subunit, PsaX2, which stabilizes the photosystem I complex and shapes pigment geometry and energetics to enable the formation of red forms. Last, we show that the protein modifications responsible for binding and tuning these red forms are widespread across the genus but not within the model MBIC11017 strain. Far-red photons lie close to the energetic limit of oxygenic photosynthesis; their efficient use therefore requires fine-tuning of the photosynthetic machinery. To our knowledge, our findings provide the structural and mechanistic basis of one of the most red-shifted photosystem I complexes identified to date, highlighting a distinct adaptive strategy in far-red light environments and offering design principles for extending photosynthesis in crops into the infrared.
History
DepositionAug 1, 2025-
Header (metadata) releaseJul 8, 2026-
Map releaseJul 8, 2026-
UpdateJul 8, 2026-
Current statusJul 8, 2026Processing site: PDBe / Status: Released

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Structure visualization

Supplemental images

emd_54627.png

Downloads & links

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Map

FileReleased
Projections & slices

Image control

Size
Brightness
Contrast
Others
AxesZ (Sec.)Y (Row.)X (Col.)
1.06 Å/pix.
x 600 pix.
= 634.8 Å		1.06 Å/pix.
x 600 pix.
= 634.8 Å		1.06 Å/pix.
x 600 pix.
= 634.8 Å

Surface

Projections

Slices (1/3)

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Slices (2/3)

Images are generated by Spider.

Voxel sizeX=Y=Z: 1.058 Å
Density

Histogram

Histogram (log scale)
Contour LevelBy AUTHOR: 0.2
Minimum - Maximum-0.5045137 - 1.6340985
Average (Standard dev.)-0.00040643223 (±0.018221866)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions600600600
Spacing600600600
CellA=B=C: 634.8 Å
α=β=γ: 90.0 °

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Supplemental data

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Half map: #2

Fileemd_54627_half_map_1.map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Half map: #1

Fileemd_54627_half_map_2.map
Projections & Slices
AxesZYX

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Slices (1/2)
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Histogram

Histogram (log scale)

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Sample components

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Entire : Photosystem I

EntireName: Photosystem I
Components
  • Complex: Photosystem I
    • Protein or peptide: Photosystem I P700 chlorophyll a apoprotein A1
    • Protein or peptide: Photosystem I P700 chlorophyll a apoprotein A2
    • Protein or peptide: Photosystem I iron-sulfur center
    • Protein or peptide: Photosystem I reaction center subunit II
    • Protein or peptide: Photosystem I reaction center subunit IV
    • Protein or peptide: Photosystem I reaction center subunit III
    • Protein or peptide: Psa27 protein
    • Protein or peptide: Photosystem I reaction center subunit IX
    • Protein or peptide: Photosystem I reaction center subunit PsaK
    • Protein or peptide: PsaL
    • Protein or peptide: PsaM
    • Protein or peptide: PsaX2
  • Ligand: CHLOROPHYLL D
  • Ligand: PHEOPHYTIN A
  • Ligand: PHYLLOQUINONE
  • Ligand: (6'S)-beta,epsilon-carotene
  • Ligand: 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE
  • Ligand: IRON/SULFUR CLUSTER
  • Ligand: 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE
  • Ligand: CHLOROPHYLL D ISOMER
  • Ligand: water

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Supramolecule #1: Photosystem I

SupramoleculeName: Photosystem I / type: complex / ID: 1 / Parent: 0 / Macromolecule list: #10, #1, #11, #2-#3, #12, #4-#9
Details: Chlorophyll d containing photosystem I from A. marina NIES-2412
Source (natural)Organism: Acaryochloris marina (bacteria)
Molecular weightTheoretical: 311 KDa

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Macromolecule #1: Photosystem I P700 chlorophyll a apoprotein A2

MacromoleculeName: Photosystem I P700 chlorophyll a apoprotein A2 / type: protein_or_peptide / ID: 1 / Number of copies: 1 / Enantiomer: LEVO / EC number: photosystem I
Source (natural)Organism: Acaryochloris marina NIES-2412 (bacteria)
Molecular weightTheoretical: 82.083172 KDa
SequenceString: MATKFPSFSQ DLAQDPTTRR IWYGIATVHD FETHDGMTEE NLYQKIFATH FGHLSIIFLW TAGLLFHVAW QGNFEQWIQD PLTVRPIAH AIWDPHLGDA ATQAFTQAGA SGPVDLCYSG VYHWWYTIGM RTNGDLYSGS IFLMVVAAVM LFAGWLHLQP K FRPSLAWF ...String:
MATKFPSFSQ DLAQDPTTRR IWYGIATVHD FETHDGMTEE NLYQKIFATH FGHLSIIFLW TAGLLFHVAW QGNFEQWIQD PLTVRPIAH AIWDPHLGDA ATQAFTQAGA SGPVDLCYSG VYHWWYTIGM RTNGDLYSGS IFLMVVAAVM LFAGWLHLQP K FRPSLAWF RDAESQMNHH LAVLFGASSL GWTGHLIHVA IPEARGQHVG WDNFLSTMPH PAGLAPFFTG RWAVYAQNPD TT GHIFGTS EGAGTAILTF VGGFHPQTEA LWLTDIAHHH LAIAVLYIVA GHMYRTQFGI GHSMKEIMDA HRDPWYGATL EGL YDTYNE SLHFQLAFHL AALGVITEVV AQHMYSLPSY AFISQDYVTQ AALYTHHQYI GGFLALGAYA HGGIFFVRDY DPER NKGNA LSRLLEHKEA IISHLSWVSM FLGFHTLDLY VHNDVVVAFG TPEKQILPEP IFAEWVQAAH GKLLLGLDSL LSNPQ SIAA TAWPNYGDVW LPGWLDAVNG SSTPFLNIGP GDFLVHHGIA FSIHVTVLIC VKGCLDARGS KLMPDKKDFG YSFPCD GPG RGGTCDISAW DSFYLATFWM LNTIGWVTFY FNWKQLSVWS GNLAQFNNDS TYLMGWLRDY LWGYSAQLIN GYTPFGV NS LSVWAWIFLL GHLCWATGFL FLISWRGYWQ ELIETLVWAH QRTPLANLVM WKDKPVALSI VQGRLVGLVH FAVGYFVT Y GAFVIGATAP LG

UniProtKB: Photosystem I P700 chlorophyll a apoprotein A2

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Macromolecule #2: Photosystem I reaction center subunit II

MacromoleculeName: Photosystem I reaction center subunit II / type: protein_or_peptide / ID: 2 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Acaryochloris marina NIES-2412 (bacteria)
Molecular weightTheoretical: 15.12515 KDa
SequenceString:
MAETLTGQTP LFGGSTGGLL SSAETEEKYA ITWTSPKQQV FEMPTGGAAV MNEGENLLYL ARKEQCLALG LRQLRIKKIM DYKIYRVLP DGSNTLLHPK DGVFPEKSNE GRAAVNSVAR SIGENPNPGA IKYTGKQAYD

UniProtKB: Photosystem I reaction center subunit II

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Macromolecule #3: Photosystem I reaction center subunit IV

MacromoleculeName: Photosystem I reaction center subunit IV / type: protein_or_peptide / ID: 3 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Acaryochloris marina NIES-2412 (bacteria)
Molecular weightTheoretical: 9.350654 KDa
SequenceString:
MVQRGSKVRI LRPESYWFRE IGTVASVDKS GIKYPVIVRF DTCNYNGISG TAAGINTNNF GVHELEEVEA PKAKAKKPAA APKAEG

UniProtKB: Photosystem I reaction center subunit IV

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Macromolecule #4: Psa27 protein

MacromoleculeName: Psa27 protein / type: protein_or_peptide / ID: 4 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Acaryochloris marina NIES-2412 (bacteria)
Molecular weightTheoretical: 3.648337 KDa
SequenceString:
MISDILPAIM TPLVVLIGGG AAMTAFFYYV EREG

UniProtKB: Psa27 protein

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Macromolecule #5: Photosystem I reaction center subunit IX

MacromoleculeName: Photosystem I reaction center subunit IX / type: protein_or_peptide / ID: 5 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Acaryochloris marina NIES-2412 (bacteria)
Molecular weightTheoretical: 5.457358 KDa
SequenceString:
PPQIDSEKGF MKFFSTAPVI AFVFFTLTAG FLVELNRFFP DILFFPY

UniProtKB: Photosystem I reaction center subunit IX

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Macromolecule #6: Photosystem I reaction center subunit PsaK

MacromoleculeName: Photosystem I reaction center subunit PsaK / type: protein_or_peptide / ID: 6 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Acaryochloris marina NIES-2412 (bacteria)
Molecular weightTheoretical: 8.894473 KDa
SequenceString:
MFTPTFLTVA VRAVNWTPTV GVVMIAANIM AIAIAKYSIQ YPNVGPSMPA SSLFGGFSFN AVLGTQVFGH VLGAGAILGL TYLGVL

UniProtKB: Photosystem I reaction center subunit PsaK

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Macromolecule #7: PsaL

MacromoleculeName: PsaL / type: protein_or_peptide / ID: 7 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Acaryochloris marina (bacteria)
Molecular weightTheoretical: 15.588004 KDa
SequenceString:
MTMEMVKHGG DPFVGDLATP VNSSGIVKAW INNLPAYRKG MSANARGLEI GMAHGYYLYG PFATSGPVRG TTMALVSGVL SASCVIIVL TVAMQLYSSL SVPKPRPSVT TADPGSDFGT KEGWSAIGSG FLIGGCGGAV IAGVLSYAIA IFAG

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Macromolecule #8: PsaM

MacromoleculeName: PsaM / type: protein_or_peptide / ID: 8 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Acaryochloris marina (bacteria)
Molecular weightTheoretical: 3.204925 KDa
SequenceString:
MELSDLQIVI ALVVALLPAL LALNLGTALS K

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Macromolecule #9: PsaX2

MacromoleculeName: PsaX2 / type: protein_or_peptide / ID: 9 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Acaryochloris marina (bacteria)
Molecular weightTheoretical: 2.973575 KDa
SequenceString:
KNPWPTLPLI WSGIGILAAI WITLQIG

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Macromolecule #10: Photosystem I P700 chlorophyll a apoprotein A1

MacromoleculeName: Photosystem I P700 chlorophyll a apoprotein A1 / type: protein_or_peptide / ID: 10 / Number of copies: 1 / Enantiomer: LEVO / EC number: photosystem I
Source (natural)Organism: Acaryochloris marina NIES-2412 (bacteria)
Molecular weightTheoretical: 83.573766 KDa
SequenceString: MTNSPGGPET KGRTAEVDIN PVKASLEVAG KPGHFNKSLS KGPQTTTWIW NLHALAHDFD TQTNDLEEIS RKIFSAHFGH LAIVFVWIS GMIFHAARFS NYYAWLADPL GNKPSAHVVW PIVGQDILNA DVGNGFHGIQ ITSGLFHILR GAGMTAPIEL Y STSIGALV ...String:
MTNSPGGPET KGRTAEVDIN PVKASLEVAG KPGHFNKSLS KGPQTTTWIW NLHALAHDFD TQTNDLEEIS RKIFSAHFGH LAIVFVWIS GMIFHAARFS NYYAWLADPL GNKPSAHVVW PIVGQDILNA DVGNGFHGIQ ITSGLFHILR GAGMTAPIEL Y STSIGALV AAAVTMYAGY FHYHKKAPKL EWFQNAEATI GGHLINLLGL GNLAWTGHLI HVSLPINKML DSGMAPEDIP IP HEYLLDK AFMADLYPSF AQGLAPYFTL NWGVYSDFLT FKGGLDPQTG GLWMTDIAHH HLALAVMYII AGHMYRTNWG IGH HMREIM DAHKGPFTGE GHKGLYDVLT TSWHAQLAIN LACWGSFSIV CAHHMYAMPP YPYLATDYAT QLNLFVHHMW LGGF YIVGG AAHAAIFMVR DYDPAMNQNN VLDRMLRHRD TIIAHLNWVC LFLGFHSFDL YIHNDEMRSL GRPQDMFSDT AIQLQ PIFA QWVQNLQANV AGTIRAPLAE GATSLAWGGD PMFVGGKVAM QHVSLGTADF MIHHIHAFQI HVTVLILLKG VLYARS SRL IPDKASLGFR FPCDGPGRGG TCQSSGWDHV FLGLFWMYNC ISIVNFHFFW KMQSDVWGTA NASGGINYLT AGNWAQS SI TINGWLRDFL WAQSVQVINS YGSALSAYGI LFLGAHFIWA FSLMFLFSGR GYWQELIESI VWAHSKLKIA PAIQPRAM S ITQGRAVGLG HYLLGGIVTS WSFYLARILA LG

UniProtKB: Photosystem I P700 chlorophyll a apoprotein A1

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Macromolecule #11: Photosystem I iron-sulfur center

MacromoleculeName: Photosystem I iron-sulfur center / type: protein_or_peptide / ID: 11 / Number of copies: 1 / Enantiomer: LEVO / EC number: photosystem I
Source (natural)Organism: Acaryochloris marina NIES-2412 (bacteria)
Molecular weightTheoretical: 8.825207 KDa
SequenceString:
MSHTVKIYDT CIGCTQCVRA CPTDVLEMVP WDGCKAGQIA SSPRTEDCVG CKRCETACPT DFLSIRVYLG AETTRSMGLA Y

UniProtKB: Photosystem I iron-sulfur center

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Macromolecule #12: Photosystem I reaction center subunit III

MacromoleculeName: Photosystem I reaction center subunit III / type: protein_or_peptide / ID: 12 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Acaryochloris marina NIES-2412 (bacteria)
Molecular weightTheoretical: 17.691422 KDa
SequenceString:
MRRLFAVLVV LTLWLGVVPP ASADIGGLVP CSESPQFQER AAKARNTTGD PESGRKRFEM YSSALCGADD GLPRIIAGGP WSRAGDFLI PGLLFIYIAG GIGNASRNYQ IANRKKNPKN PAMGEIIIDV PLALSSTIAA LAWPLKALGE VTSGKLTVPD S DVTVSPR

UniProtKB: Photosystem I reaction center subunit III

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Macromolecule #13: CHLOROPHYLL D

MacromoleculeName: CHLOROPHYLL D / type: ligand / ID: 13 / Number of copies: 93 / Formula: CL7
Molecular weightTheoretical: 895.462 Da
Chemical component information

ChemComp-CL7:
CHLOROPHYLL D

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Macromolecule #14: PHEOPHYTIN A

MacromoleculeName: PHEOPHYTIN A / type: ligand / ID: 14 / Number of copies: 2 / Formula: PHO
Molecular weightTheoretical: 871.2 Da
Chemical component information

ChemComp-PHO:
PHEOPHYTIN A

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Macromolecule #15: PHYLLOQUINONE

MacromoleculeName: PHYLLOQUINONE / type: ligand / ID: 15 / Number of copies: 2 / Formula: PQN
Molecular weightTheoretical: 450.696 Da
Chemical component information

ChemComp-PQN:
PHYLLOQUINONE

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Macromolecule #16: (6'S)-beta,epsilon-carotene

MacromoleculeName: (6'S)-beta,epsilon-carotene / type: ligand / ID: 16 / Number of copies: 21 / Formula: A1JPJ
Molecular weightTheoretical: 536.873 Da

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Macromolecule #17: 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE

MacromoleculeName: 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE / type: ligand / ID: 17 / Number of copies: 1 / Formula: LMG
Molecular weightTheoretical: 787.158 Da
Chemical component information

ChemComp-LMG:
1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE

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Macromolecule #18: IRON/SULFUR CLUSTER

MacromoleculeName: IRON/SULFUR CLUSTER / type: ligand / ID: 18 / Number of copies: 3 / Formula: SF4
Molecular weightTheoretical: 351.64 Da
Chemical component information

ChemComp-FS1:
IRON/SULFUR CLUSTER

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Macromolecule #19: 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE

MacromoleculeName: 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE / type: ligand / ID: 19 / Number of copies: 2 / Formula: LHG
Molecular weightTheoretical: 722.97 Da
Chemical component information

ChemComp-LHG:
1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE / phospholipid*YM

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Macromolecule #20: CHLOROPHYLL D ISOMER

MacromoleculeName: CHLOROPHYLL D ISOMER / type: ligand / ID: 20 / Number of copies: 1 / Formula: G9R
Molecular weightTheoretical: 895.462 Da
Chemical component information

ChemComp-G9R:
CHLOROPHYLL D ISOMER

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Macromolecule #21: water

MacromoleculeName: water / type: ligand / ID: 21 / Number of copies: 39 / Formula: HOH
Molecular weightTheoretical: 18.015 Da
Chemical component information

ChemComp-HOH:
WATER

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

BufferpH: 6.5
Details: 50 mM MES, 1 M betaine monohydrate, 20 mM CaCl2, 5 mM MgCl2 and 10% (v/v) glycerol, pH 6.5 adjusted with NaOH
VitrificationCryogen name: ETHANE / Chamber humidity: 95 % / Chamber temperature: 277 K / Instrument: FEI VITROBOT MARK IV

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Electron microscopy

MicroscopeTFS KRIOS
Image recordingFilm or detector model: GATAN K3 BIOQUANTUM (6k x 4k) / Average electron dose: 50.0 e/Å2
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: SPOT SCAN / Imaging mode: BRIGHT FIELD / Cs: 2.7 mm / Nominal defocus max: 2.0 µm / Nominal defocus min: 0.8 µm
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

CTF correctionType: PHASE FLIPPING AND AMPLITUDE CORRECTION
Startup modelType of model: NONE
Final reconstructionResolution.type: BY AUTHOR / Resolution: 2.44 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: cryoSPARC / Number images used: 151833
Initial angle assignmentType: MAXIMUM LIKELIHOOD
Final angle assignmentType: MAXIMUM LIKELIHOOD
FSC plot (resolution estimation)

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